Food Safety Online Ordering

Water Test System www.neogen.com/foodsafetyorder.htm

Colitag®
Presence/Absence (P/A) Water Test Kit
Results

Negative for Positive for Positive for

coliforms coliforms E. coli

Product Specifications
Sensitivity: 1 CFU in a 100 mL sample
Testing time: 24 ± 2 hours
Intended Use
Storage: 2–30°C
The Colitag P/A Water Test Kit uses an EPA-approved selective and
Tests per kit: 20, 100 or 250
differential medium to detect total coliforms and E. coli in water samples
in 24 ± 2 hours. Approvals: EPA (http://www.epa.gov/fedrgstr/
EPA-WATER/2004/February/Day-
The Test 13/w3226.htm)
A 100 mL water sample is mixed with medium, followed by incubation
at 35º C for 24 ± 2 hours. If coliform bacteria are present, the medium Materials Provided
changes color from near colorless to a vibrant yellow. In addition, if any 1. Colitag P/A test kits, 100 mL format
E. coli are present, a bright blue fluorescence is seen when the sample
Materials Recommended But Not Provided
is subjected to UV light. Colitag detects enzymes characteristic of
E. coli and the coliform groups. Identification of these enzymes is ac- 1. Incubator or water bath capable of maintaining 35°C + 0.5°
complished by resuscitation of the target organisms using a combined 2. Long wavelength (366 nanometer) UV lamp
low pH buffered medium and nutrients that resuscitate weak or injured 3. Sample containers large enough to contain 100 mL water
E. coli cells. samples
Optional
Test Procedure
1. Incubator or water bath capable of maintaining 44.5°C + 0.2°
1. If necessary, neutralize chlorine in the sample by adding 10 mg
2. Kovac's reagent
of sodium thiosulfate to 100 mL of chlorinated water samples.
3. Sodium thiosulfate
2. Aseptically add Colitag to a 100 mL test water sample. Agitate
to dissolve.
3. Incubate the sample with medium at 35°C ± 0.5° for 24 ± 2
hours.
Interpretation of Results
1. Visually check each sample for vibrant yellow color. If the test
sample is yellow, coliform bacteria are present. Ordering Information
2. If the solution is yellow, examine it for fluorescence using a long Prod#. Product description
wavelength UV lamp. If a bright blue fluorescence is observed, 9850 Colitag P/A water test kits, 20 pack
this indicates that MUG-positive E. coli bacteria are present. 9851 Colitag P/A water test kits, 100 pack
3. If no yellow color in the test sample is observed after the incuba- 9852 Colitag P/A water test kits, 250 pack
tion, the sample is negative. 9854 Kovac's reagent, 25 mL
9855 Sample containers with sodium thiosulfate, 100 pack
See package insert for complete instructions and an optional fecal coliforms
Sterile fliptop cap and lock polypropylene containers with
procedure
labels
9856 Sample containers without sodium thiosulfate, 100 pack
Sterile fliptop cap and lock polypropylene containers with
labels

Sales & Service: 800/234-5333 (USA/Canada) or 517/372-9200 • Fax: 517/372-2006 • www.neogen.com 57