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GENE-TRAK®
Generic E. coli Assay
Results
Product Specifications
Sensitivity: 1-5 CFU/25 g
Testing time: 2 hours (after 24-48 hour
enrichment)
Tests per kit: Up to 98
Intended Use
GENE-TRAK E. coli Assay provides for the detection of E. coli in food. Materials Required But Not Provided
Available from Neogen*
The Test 1. GENE-TRAK photometer set
The DNA hybridization test employs E. coli-specific DNA probes and a 2. Pipettes (1 mL and 10 mL)
colorimetric detection system for the detection of E. coli in food samples 3. Incubator at 35°C
following broth culture enrichment. The method is a semi-quantitative 4. 12 x 75 mm glass test tubes
test to detect the presence of E. coli at contamination levels greater than 5. Minute timer
3 cells per gram in the original food sample. A sample is considered 6. Micropipette and tips, 100-1000 mL range (optional)
non-reactive for the presence of E. coli if the absorbance value obtained 7. Test tube racks
is less than or equal to the established cutoff value for the assay. A sample 8. Dipstick holders
is considered reactive for the presence of E. coli if the absorbance value 9. Wash basins w/lids
obtained is greater than the established cutoff value of the assay. 10. Dilution chart (for enzyme conjugate)