PARASITOLOGY LABORATORY 9 – Protozoans

USTMED ’07 Sec C – AsM; Photos provided by JV.N.

ENTAMOEBA HISTOLYTICA

E. Histolytica Cyst

ENTAMOEBA COLI

Mature cysts of E. histolytica have four nuclei while immature

E. coli – Iodine Stained
cysts contain one or two nuclei. The cysts range in size from 10 to
18 um and frequently have a diffuse glycogen vacuole, as in this
case. Red chromatoid bodies with rounded ends are frequently
found. The karyosomes may be slightly eccentrically located, and
the peripheral chromatin is fine and evenly distributed.

E. Histolytica Trophozoites

E.coli trophozoite with nucleus

in which the karyosome
appears large and irregular

E. coli trophozoite with nuclei

in which the karyosome is
eccentric, cytoplasm is
vacuolated
Entamoeba histolytica.

Trophozoites. Feces. Trichrome Entamoeba histolytica cyst

stain. The trophozoites of E.
histolytica are round and measure
from 15 to 30 um in diameter. With
the trichrome stain the cytoplasm
stains green-magenta, and the
nucleus has a central karyosome.
The chromatin has a delicate
appearance and is distributed
uniformly in the periphery along the nuclear membrane.

Entamoeba histolytica.

ENTAMOEBA HARMANII

Entamoeba harmanii

Trophozoites. Feces. Trichrome stain. This E. histolytica

trophozoite shows an ingested red blood cell in the cytoplasm.
The nuclear structure is still fairly typical although the karyosome
is eccentrically located. Ingestion of red blood cells by E. Typical E. harmanii trophozoites are small, and characteristically
histolytica occurs in patients with invasive disease and is take a light stain. The nucleus is small, has a discrete central
considered to be a characteristic that distinguishes this organism karyosome, and fine peripheral chromatin. The cytoplasm, which
from the other species of Entamoeba. In addition to red cells, usually is finely granular, never contains red blood cells.
bacteria and cell debris can be found in the phagosomes of this
parasite. Macrophages that have ingested cellular material can
sometimes be confused with E. histolytica. ENDOLIMAX NANA

Endolimax nana cyst

Amoebiasis Intestine
IODAMOEBA BUTSCHLII

Iodamoeba Butschlii Trophozoites

Trichrome-stained trophozoites
demonstrates characteristic
vacuolations of the cytoplasm and
the typical nucleus containing
large, dark staining karyosome.
Nuclear membrane lacks
peripheralchromatin.

Iodamoeba butschlii cyst

The most characteristic feature

of Iodamoeba cysts is its large
glycogen vacuole clearly seen in
this trichrome-stained organism.
The cyst are invariably
uninucleate and has a typical
large karyosome.

Iodamoeba butschlii

Iodameoba butschlii Trophozoites Cysts

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