Beruflich Dokumente
Kultur Dokumente
Total period 180 (Theory 113, Practice 45, Individual sdudy 22.)
Chapter Contents Total Theoy
period
Chapter 1 Introduction, Structures and Functions of Proteins 12 7 5
Intruduction to Biochemistry
1
Biochemistry is the study of life on the molecular level.Therefore, biochemistry, as the name implies, is the
chemistry of life. Life, at its most basic level, is a biochemical phenomenon including two basic characteristics: self-
refresh (metabolism), and self-replication and self-assembly (expression and transmission of genetic information).
The 21th century is an era of life science. Lots of wonders are being created, and explosive information is
being provided at an unprecedented speed. Biochemistry is a window opening to the world of life science. Thus, the
knowledge of biochemistry which involves the study of chemical molecules and reactions in living organisms, and
the elucidations of the nature of live phenomenon on the molecular level, is essential to medical students, as well as
to the students of other related disciplines.
As is known to all, when biochemistry is taught in English, it will be double-beneficial to the chinese students
and the students abroad, for they get trained in their professional language while learning biochemical knowledge.
This is not only significant to students who want to expand their knowledge from other sources written in English,
but also helpful to the students who are going to pursue their scientific career in the future.
There are four parts in this program, and totally 16 chapters: the structures and
functions of biomolecules ( Part I); the metabolism of substrates and their regulations (Part II); expression and
transmission of genetic information, Gene Recombination & Gene Ingeneering (Part III); comprehensive topics
(Part IV). Totally 180 teaching hours, including 113 for theory and 45 for experiment and 22 individual sdudy.
In this syllabus, the contents of 16 chaptesr have been pointed out what kind
of demands should be met in different classes. That is----- emphasis means to memorize the knowledge basing on
the understanding of the principle and to solve problems and practice applying the principle; difficulty means: not
the emphasis but understand.
Chapter 4 Vitamins 4
1. Master the structure,coenzymes form and fungction of vitamins of B complex (water-soluble vitamins):
VitaminB1, B2, pentothenic acid(B5), PP, B6, biotin, folic acid(B11), and B12.
2. Know well the function of Vitamin A , D , E ,and K ; Know well the function of Vitamin C.
3. Understand the vitamin deficiency disease.
Emphasis: coenzymes form and function of vitamins of B complex.
Difficulty: the structure of vitamins.
Contents: Vitamin is defined as an essential, noncaloric, organic nutrient needed in tiny amounts in the diet. The
role of many vitamins is to help make possible the processes by which other nutrients are digested, absorbed, and
metabolized or built into body structures. Although small in size and quantity, the vitamins accomplish mighty tasks,
some of which are still being discovered.
.
The Teaching of Practice
Experiment 1 Protein determination by the biuret reaction 5 hours
1. Emphasis
(1).Master the rules of biochemical laboratory and the basic contents of the experimental report
(2).Master the principle of spectrophotometry
(3).Master the biuret method to determine protein
2. Contents of practice
(1). Plot the standard curve
(2). Unkown sample determination
3. Homework
Experiment 2 The effect of substrate concentration on the activity of alkalin phosphatase and
9
thedetermin –action of Km of alkaline phosphatase (Its abbreviation is AKP) 5 hours
1.Master the method of determination of Km of alkaline phosphatase
2. Master the significance of Km
3. Master the effect of substrate concentration on the activity of AKP
4. Known well how to design an experiment of enzyme
Homework: Make Linewearver-Burk plot with 1/[S] as abscissa and 1/V as the ordinate (of 1/v versus 1/[S]), Read
1/Km from the beeline,then calculate Km.
Experiment 3 The Effect of Adrenalin and Insulin on Concentration of Blood Sugar 5 hours
1. Emphasis:
(1) Master the method of determination concentration of blood sugar
(2) Know well the effect of adrenalin and insulin on concentration of blood sugar
2. Contents of practice
(1)Separately weigh 2 rabbits
(2)Separately draw the blood from 2 rabbits
(3)Inject insulin and adrenaline
(4)Again separately draw the blood
(5)Prepaire protein—free filtrates
(6)Determination of Glucose by the method of Folin and Wu.
(7)Result
Reference Books :
1. Zhou Airu. 2004. Biochemistry (Chinese). 6th Edition. Beijing: Renmin
Weisheng publisher
2. Berg J M,Tymoczko J L,Stryer L. 2002. Biochemistry. 5th Edition. New York:W.H. Freeman Publishers
3. Chytil F, Mccmick D B .1986. Vitamins and coenzymes. New York: New York Halsted Press
4. Devlin T M.. 2002. Textbook of Biochemisrty with Clinical Correlations, New York: Wiley-Liss
5. Robert K. Murray, Daryl K. Granner, Peter A. Mayes , et al. 2000. Harper’s Biochemistry. 25th Edition.
Stamford:McGraw-Hill
11