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Exam1 Name:_____________________ April11,10:00am Closedbook,closednotes.Onlyonepageofnotesandacalculatorareallowed.Youcangive youranswerinChinese. I. Giveashortanswertoeachofthefollowingproblems. 1. (7%)ShownbelowisthestructureofanIgGmolecule.Forpharmaceutical applications,aretheyproducedinanimalcellsorinbacteria?Whatstructural characteristicsofIgGdictatetheselectionofproducingcells?

FcRegion

2. (6%)ListthreebiotechnologyproductsproducedinTaiwan

3. (10%)MostbacteriaconvertallofthenitrogenatomstheytakeupfromNH3 completelytocellularnitrogen(inproteins,nucleicacids,etc).Theyieldbasedon nitrogenisthereforetheinverseofnitrogencontentinthecells(i.e.,ifthecellhas 0.08 g nitrogen/g DCW thentheyieldonNH3is(1/0.008gDCW/gN)(14gN/17g NH3)=10.3gDCW/gNH3).However,evenifyouknowcellscarboncontent,youcant estimatetheyieldcoefficientbasedonthecarbon/energysource,why?(DCW:dry cellweight)

CalculationProblems 1. (12%)Shownbelowisagraphofgrowthkineticsofamicroorganism.Giveaquantitative estimateofthespecificgrowthrateandspecificsubstrateconsumptionrateduring exponentialgrowthphase. II.

2. (20%)Ayeastcellgrowingonglucosehasamaximumspecificgrowthrateof0.45h1.The yieldcoefficientis0.4gcelldryweight/gglucose.Usingafeedstreamwith80g/lofglucose, acontinuousculturewillbesetuptoproduce40,000kgperyearofdryyeastcells.Theplant willbeoperated340daysayear.Whatsthesizeofthereactorneeded?Makenecessary assumptionsandclearlystateyourassumptions.Iftheproblemdoesnotspecifysufficient conditionstogiveadefinitiveanswer,assignavaluetoconditionandstatewhyyouchoose thatcondition.Useagoodpracticeofprocesstechnologist,neglecttermswhosevalueis verysmallcomparedtoothers. 3. (20%)Acontinuouscultureisoperatedatsteadystateusingglucoseasalimitingsubstrate. The conditions are (So (feed glucose)=20 g/l; S=0.02 g/l ; x=10 g/l; D=0.25 h1). Now D is changedto0.3h1andSoto15g/l.ThenewDisstillatleast10%belowmaximumspecific growth rate. After the new steady state is reached, will S and x increase or decrease respectively?Why?Estimatexatthenewsteadystate.Assumethegrowthisdescribedby Monodkinetics.

4. (25%)Abioreactorsetupisshownbelow.Tomeasureoxygenconsumption,asmallstream of culture medium (with cells) is taken out and passed through a small (volume=0.1l) chamber.Attheinletandoutletofthechamberoxygenconcentrationismeasured.Theflow rateis6l/hr,thecellconcentrationis10g/l.Theinletandoutletoxygenconcentrationis0.1 mmol/land0.05mmol/lrespectively. a.) (25%)Whatisthespecificoxygenconsumptionrate?

Dothispageafterexam.Itwillbepostedontheweb.YoucanturninApril 18classifyouwishtogetbonuspoints. (10%bonus)Insteadofusingthechamberandflowthecellsuspension throughnowyouputthecellsuspensioninthechamber.Thechamberis filledwithcellsuspension(noair).Thenyoumeasurethedecreaseof oxygeninthesuspensionovertime(inotherwords,inabatchmanner,no flowthrough).Plotthedissolvedoxygenconcentrationovertime quantitatively. Howdoyoucalculatespecificoxygenconsumptionrate? Willyouobtainthesamevalueofspecificoxygenconsumptionrateasin a.)?

b.)