Beruflich Dokumente
Kultur Dokumente
A lf Lhe daughLer marrles a normal male Lhe probablllLy of a daughLer havlng
hemophllla ls
B. About of male children would have hemophilia (Boxes 2 and 4 above)
C. The probability that all 4 sons have inherited hemophilia would be: 1/2 x 1/2 x
1/2 x 1/2 or 1/16.
Molecular Cenetics: Problem
9seoJobypetttoplc moscolot Jysttopby ls o JlsotJet tbot cooses qtoJool Jetetlototloo of tbe moscles
lt ls seeo ooly lo boys boto to oppoteotly ootmol poteots ooJ osoolly tesolts lo Jeotb lo tbe eotly
teeos (o) ls pseoJobypetttopblc moscolot Jysttopby cooseJ by o Jomlooot ot tecesslve ollele? (b) ls
lts lobetltooce sexllokeJ ot ootosomol? (c) now Jo yoo koow? xplolo wby tbls JlsotJet ls olwoys
seeo lo boys ooJ oevet qltls
(a) seudohyperLroplc muscular dysLrophy ls a recesslve allele because lf lL were
domlnanL Lhen Lhe heLerozygous female would dle before she was sexually
maLure and Lhus could noL pass on Lhe LralL
(b) Pseudohypertropic muscular dystrophy is a sex-linked trait because it only
occurs in males.
(c) It is never seen in girls because Ior a girl to be homozygous recessive (and
express the trait) XmXm, her Iather would have to be hemizygous recessive XmY)
and her mother would have to be heterozygous or homozygous recessive (X
m
X
M
or
X
m
X
m
). It is impossible Ior a hemizygous recessive male or a homozygous
recessive Iemale to have children because they will die beIore being old enough to
have children.
1he unneLL square above shows LhaL ln a cross beLween Lwo apparenLly normal
parenLs buL Lhe moLher ls a carrler Lhree chlldren wlll be normal buL one always
a boy wlll lnherlL Lhe dlsease
ProbIem 8.1
How many fragments would be generated by a restriction endonuclease in a plasmid that has two recognition sequences?
Because plasmids are circular molecules, two fragments would be generated. However, if the DNA were linear and contained
two recognition sequences, three fragments would be generated.
ProbIem 8.2
Suppose the restriction endonuclease Hind cuts a 6.0 kb linear piece of DNAinto two fragments; an 800 bp fragment and a
5200 bp fragment. Nar cuts the DNA also into two fragments; fragments 1200 and 4800 bp long. Relative to the Hind cut site,
there are two possible ways in which Nar could have cut the DNA. How can you determine the correct cleavage site for Nar
with respect to Hind?
To determine this, one must subject the DNA to a double digest in which both the enzymes are allowed to cut the DNA at the
same time.When the double digest is allowed to take place, if the three fragments that appear upon electrophoresis of the
restricted DNAare 400, 800, and 4800 bp, then the only possible way in which the data can be interpreted is with the 1200 bp
Nar fragment containing the Hind recognition site 800 bp from the end of the linear piece of DNA. f the 4800 bp Nar
fragment contained the cut site, you would visualize fragments of sizes 800, 1200, and 4000 bp after electrophoresing the
doubly digested DNA.