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THE BOMBAY TEXTILE RESEARCH ASSOCIATION

LAl BAHADUR SHASTRIMARG, GHATKOPAR, (WEST),
MUMBAI 086 400 PHONES: 25001811, 25003651/2652/2117/1119/1135 Fax: 91-22-25000459, E-mail: btra@ysnl.com

MICROBIOLOGY I BIOTECHNOLOGYDIVISION
Client's Name: LabNo. MIs.MicrotrolSterilisation Reference: ServicesPvt. Ltd. Date: Your LetterNo.M:06:082 Dated: 20.07.2005 Sample Received : on
Test Material

MUS31 25.07.2005 1 of 1
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21.07.2005
:

TotalPages:

wrC/34 WHITE ONION POWDER"A" 1 - 725 cartons ODF/WTC/34/09/05 Treated with Ethylene oxide Batch # 5464 dated 16.07.2005

Dateof Test Typeof Test

21.07.05 1. Total PlateCount 2. TotalYeastand MoldCount 3. Detection Enumeration Coliforms & of 4. Detection Escherichia of coli 5. Detection Salmonella of

Test Method Used:

1. ANSI/AAMI/ISO 11137-1995 2. ASTA Microbiological Methods:M-2.0;M-4.0;M-6.0; M- 8.0

3. ASTA Microbiological Methods M - 3.1 M - 3.2 &
MPN Tube Method

IncubationConditions

30 - 35C for 2- 3 Days

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ForBacteria
Incubation Conditions For Yeastand Molds 28Cfor 3 - 5 Days

Results : 1. Total Plate Count 2. Total Yeast and Mold Count: 3. Detection of Coliforms Coliform MPNper Pfam 4. Detection of Escherichia coli: 5. Detection of Salmonella

: 2.70x 104c.f.u./gram Sample of 1.5 x 103c.f.u./gram Sample of : Present per gramof Sample : 240 Absent per gramof Sample : Abs,nt per 25 gramsof Sample

Conclusion: This is to certifythat wrC/34; WHITEO~}ON POWDER "A"; 1 - 725 cartons;ODF/wrC/34/

09/05; Treated with Ethylene o~ide; B~tc # 5464 dated 16.07.2005 has Total Plate Count of 2.70 x 104 c:~.u./g~am of sampl~ and To~1 Yeast and Mold Count of 1.5 x 103 c.f.u./gram of sample. Coliforms are detect~d; Colif rms MPN per gram of 240. Sample is free from Escherichia coli per gram of sample and Salmonella per 25 grams of sample when ana lysed by ANSI/AAMI and ASTA ;~~~~!~;~,e . \\\C t..

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THE BOMBAY TEXTILE RESEARCH ASSOCIATION

LALBAHADUR SHASTRI MARG, GHATKOPAR, (WE5T), MUMBAI 086 400 PHONES: 25001811, 25003651/2652/2117/1119/1135 Fax: 91-22-2500 0459,E-mail: btra@vsnl.com

MICROBIOLOGY
Client'sName:
Reference:

BIOTECHNOLOGY DIVISION
: : MUS 32 25.07.2005

MIs. Microtrol Sterilisation Lab No. Services Pvt. Ltd. Your Letter No. M:06:082Dated : 20.07.2005 Date

Sample Received on: Test Material

21.07.2005

Total Pages:

1 of 1

WTC/34
WHITE ONION POWDER "A" 1 - 725 cartons QDF/WTC/34/09/06 Treated with Ethylene oxide Batch # 5465 dated 16.07.2005

Date of Test
Type of Test

:
:

21.07.05
1. Total Plate Count 2. Total Yeast and Mold Count" 4. Detection of Escherichia coli 5. Detection of Salmonella

~,
'

3. Detection& Enumerationof Coliforms

Test Method Used:

1. ANSI/MMI/ISO 11137-1995 2. AST A Microbiological Methods :M-2.0; M-4.0;M-6.0; M- 8.0 3. AST A Microbiological Methods M - 3.1 & M - 3.2 MPN Tube Method

Incubation Conditions:
For Bacteria Incubation Conditions' For Yeast and Molds

30 - 35C for 2- 3 Days

28C for 3 - 5 Days

Results :
1. Total plate Count
: 3.70 x 104c.f.u./gram of Sample

2. Total Yeast and Mold Count: 3. Detection of Coliforrns : Coliform MPN per gram : 4. Detection of Escherichia coli: 5. Detection of Salmonella :

6.2 x 103 c.f.u./gram of Sample Present per gram of Sample 1100 Absent per gram of Sample Absent per 25 grams of Sample

Conclusion: This is to certify that WTC/34; WHITE ONION POWDER "A"; 1 - 725 cartons; QDF/ WTC/34/ 09/06; Treated with Ethylene oxide; Batch # 5465 dated 16.07.2005 has Total Plate Count of 3.70 x 104 c.f.u./gram of sample and Total Yeast and Mold Count of 6.2 x 103 c.f.u./gram of sample. Coliforms are detected; Coliforms MPN per gram of 1100. Sample is free from Escherichia coli per gram of sample and Salmonella per 25 grams of sample when analysed by ANSI/MMI and ASTA Test Methods. ...
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Dr (Mrs) Shilpa U. Nair rlnclpa nves Igator Microbiology/Biotechnology Division

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THE BOMBAY TEXTILE RESEARCH ASSOCIATION

LAl BAHADUR SHASTRIMARG, GHATKOPAR, (WEST), MUMBAI 086 400 PHONES: 25001811, 25003651/2652/2117/1119/1135 Fax:91-22-2500 0459,E-mail btra@vsnl.com :

MICROBIOLOGY I BIOTECHNOLOGYDIVISION
Client'sName: MIs. Microtrol Sterilisation Lab No. Reference: Services Pvt. Ltd. Your Letter No. M:06:082Dated 20.07.2005 Date :
Sample Receivedon: Test Material 21.07.2005 . Total Pages:

. .

MUS 33
25.07.2005

1 of 1

WTC/34 WHITE ONION POWDER "A" 501 - 725 cartons ODF/WTC/34/09/05 UNTREATED 21.07.05 1. Total Plate Count 2. Total Yeast and Mold Count 3. Detection & Enumerationof Coliforms

Date of Test Type of Test

: :

'

4. Detection Escherichia of coli 5. Detection Salmonella of Test MethodUsed: 1. ANSI/AAMI/ISO 11137-1995 2. AST Microbiological A Methods:M-2.0;M-4.0;M-6.0; M- 8.0 3. ASTA Microbiological MethodsM - 3.1 M - 3.2 & MPN Tube Method 30 - 35Cfor 2- 3 Days 28Cfor 3 - 5 Days

Incubation Conditions. For Bacteria Incubation Conditions. For Yeastand Molds

Results : 1. Total Plate Count : 2.10x 10sc.f.u./gram Sample of 2. Total Yeast and Mold Count: 9.2 x 103 c.f.u./gram Sample of 3. Detection of Coliforms : Present per gramof Sample Coliform MPN per gram: > 1100 4. Detection of Escherichia coli: Absent per gramof Sample 5. Detection of Salmonella : Absent per 25 gramsof Sample
This is to certify that WTC/34; WHITE ONION POWDER "A"; 501 - 725 cartons; ODF/ WTC/

Conclusion:

34/09/05;UNTREATED Total Plate Count of 2.10 x 105c.f.u./gramof sampleand Total has Yeast and Mold Count of 9.2 x 103c.f.u./gramof sample.Coliformsare detected;Coliforms MPN per gram of > 1100. Sample is free from Escherichiacoli per gram of sample and Salmonellaper 25 grams of samplewhen analysedby ANSI/AAMIand ASTA Test Methods. . ,.
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)1alA~--Dr (Mrs)ShilpaU. Nair :

Microbiology/Biotechnology Division

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Principal Investigator

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THE BOMBAY TEXTILE RESEARCH ASSOCIATION
LALBAHADUR SHASTRI MARG, GHATKOPAR, (WE5r), MUMBAI 086 400 PHONES: 25001811, 25003651/2652/2117/1119/1135 Fax: 91-22-2500 0459,E-mail: btra@vsnl.com

MICROBIOLOGY I BIOTECHNOLOGYDIVISION
(

Client'sName:
Reference:

en Isa Ion Services Pvt. Ltd. Date: Your Letter No. M:06:082Dated : 20.07.2005 21.07.2005 :

MI

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M.

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Lab No.

MUS 34 25.07.2005 1 of 1

Sample Receivedon : Test Material

Total Pages:

WTC/35 WHITE ONION POWDER "A" 1 - 645 cartons QDFiWTC/35/10/06 Treated with Ethylene oxide Batch # 5466 dated 16.07.2005 21.07.05
1. Total Plate Count
'

Date of Test
Type of Test

:
:

2. Total Yeast and Mold Count 3. Detection & Enumerationof Coliforms 4. Detection of Escherichia coli 5. Detection of Salmonella Test Method Used: 1. ANSI/AAMI/ISO 11137-1995 2. ASTA MicrobiologicalMethods :M-2.0; M-4.0;M-6.0; M- 8.0 3. ASTA Microbiological Methods M - 3.1& M 3.2 MPN Tube Method

Incubation Conditions: For Bacteria Incubation Conditions: For Yeast and Molds

30 - 35C for 2- 3 Days 28C for 3 - 5 Days

Results :
1. Total Plate Count 2. Total Yeast and Mold Count: 3. Detection of Coliforms Coliform MPN per gram 4. Detection of Escherichia coli: 5. Detection of Salmonella : 3.28 x 104c.f.u./gram of Sample 2.8 x 103c.f.u./gram of Sample : Present per gram of Sample : 23 Absent per gram of Sample : Absent per 25 grams of Sample

Conclusion: This is to certifythat WTC/35; WHITE ONION POWDER "A"; 1 - 645 cartons; QDF/ WTC/35/ 10/06; Treated with Ethylene oxide; Batch # 5466 dated 16.07.2005 has Total Plate Count of 3.28 x 104 c.f.u./gram of sample and Total Yeast and Mold Count of 2.8 x 103c.f.u./gram of sample. Coliforms are detected; Coliforms MPN per gram of 23. Sample is free from Escherichia coli per gram of sample and Salmonella per 25 grams of sample when analysed by ANSI/AAMI and ASTA Test Methods. "

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Dr(Mrs)Shilpa U. Nair
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