PROJECT For Structural Biology and Secondary Metabolites 2011-2012

Submitted By:
Name: PRIYANSHI GIRI
Mishra

Submitted To:
Dr. Pankaj.K.

Programme: Msc. Biotechnology Email: priyanshi.giri@gmail.com Mob.: 8826267823

Date of Submission:11-11-2011

Department of Biotechnology School of Engineering and Technology

Sharda University, Greater Noida Research Article 01

SECTION A

(1.5)

Title: Cardiac differentiation of embryonic stem

cells by substrate immobilization of insulin-like growth factor binding protein 4 with elastin-like polypeptides

Name of Authors: Ayaka Minato a, Hirohiko Ise b,*,

Mitsuaki Goto c, Toshihiro Akaike b

Affiliation: a) Graduate School of Bioscience and

Biotechnology, Tokyo Institute of Technology, B-57, 4259 Nagatsuta-cho, Midori-ku, Yokohama 2268501, Japan b )Frontier Research Center, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8503, Japan c )Celagix Res Ltd., 5800-3 Nagatsuta-cho, Midoriku, Yokohama 226-0026, Japan

Name of Journal: BIOMATERIALS Impact Factor: 7.882 Year in which article published: 2011 Volume: 33

Issue: 2012 Page No.:515-523

SECTION B

(1.5)

Objectives: i. a useful strategy for cardiovascular regenerative

medicine. ii. we report a strategy for cardiomyocyte differentiation of ESCs using substrate immobilization of insulin-like growth factor binding protein 4 (IGFBP4) with elastin-like polypeptides.

SECTION C
Statistical model used for data analysis: Cell

(1.5)

experiments were performed in triplicate, and the results were expressed as mean _ standard deviation (SD). Students t test was used to assess the statistically significant differences in the results. A probability (p) value of <0.01 was considered statistically significant

Principle: It was indicated that IGFBP4 was stably

immobilized to polystyrene dishes by the fusion of (GVGVP)67 because the number of GVGVP repeats needed to be more than 67 in order to keep the hydrophobic interaction with the dishes at 37 _C.

SECTION D

(1.5)

Summary: It was indicated that IGFBP4 was stably

immobilized to polystyrene dishes by the fusion of (GVGVP)67 because the number of GVGVP repeats needed to be more than 67 in order to keep the hydrophobic interaction with the dishes at 37 _C. Moreover, it was suggested that (GVGVP)67IGFBP4 that was immobilized to the dishes could inhibit the Wnt/b-catenin signaling more strongly and continuously than IGFBP4 as a soluble factor.These results demonstrated that cardiomyocyte differentiation of ESCs was effectively promoted by strong and continuous inhibition of Wnt/b-catenin signaling with (GVGVP)67-IGFBP4. Substrate immobilization of soluble factors with elastin-like polypeptides might lead to the development of useful strategies for regenerative medicine of various tissues such as heart, liver, and neuron.