Cell culture

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Cell culture in a Petri dish

Epithelial cells in culture, stained for keratin (red) and DNA (green) Cell culture is the complex process by which cells are grown under controlled conditions. In practice, the term "cell culture" has come to refer to the culturing of cells derived from singlecellular eukaryotes, especially animal cells. However, there are also cultures of plants, fungi and microbes, including viruses, bacteria and protists. The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Animal cell culture began when scientists removed a small amount of monkey kidney from a host organism and maintained it in a warm saline solution for several days, establishing the principle of tissue culture.[1]

Contents

1 Concepts in mammalian cell culture o 1.1 Isolation of cells o 1.2 Maintaining cells in culture o 1.3 Cell line cross-contamination o 1.4 Manipulation of cultured cells 1.4.1 Media changes 1.4.2 Passaging cells 1.4.3 Transfection and transduction

1.5 Established human cell lines 1.6 Generation of hybridomas 2 Applications of cell culture o 2.1 Cell culture in two dimensions o 2.2 Tissue culture and engineering o 2.3 Vaccines 3 Culture of non-mammalian cells o 3.1 Plant cell culture methods o 3.2 Bacterial and yeast culture methods o 3.3 Viral culture methods 4 Common cell lines 5 List of cell lines 6 See also 7 References and notes 8 External links

o o

[edit] Concepts in mammalian cell culture

[edit] Isolation of cells
Cells can be isolated from tissues for ex vivo culture in several ways. Cells can be easily purified from blood; however, only the white cells are capable of growth in culture. Mononuclear cells can be released from soft tissues by enzymatic digestion with enzymes such as collagenase, trypsin, or pronase, which break down the extracellular matrix. Alternatively, pieces of tissue can be placed in growth media, and the cells that grow out are available for culture. This method is known as explant culture. Cells that are cultured directly from a subject are known as primary cells. With the exception of some derived from tumors, most primary cell cultures have limited lifespan. After a certain number of population doublings (called the Hayflick limit), cells undergo the process of senescence and stop dividing, while generally retaining viability. An established or immortalized cell line has acquired the ability to proliferate indefinitely either through random mutation or deliberate modification, such as artificial expression of the telomerase gene. Numerous cell lines are well established as representative of particular cell types.

[edit] Maintaining cells in culture

Cells are grown and maintained at an appropriate temperature and gas mixture (typically, 37C, 5% CO2 for mammalian cells) in a cell incubator. Culture conditions vary widely for each cell type, and variation of conditions for a particular cell type can result in different phenotypes being expressed. Aside from temperature and gas mixture, the most commonly varied factor in culture systems is the growth medium. Recipes for growth media can vary in pH, glucose concentration, growth factors, and the presence of other nutrients. The growth factors used to supplement media are often derived from animal blood, such as calf serum. One complication of these

blood-derived ingredients is the potential for contamination of the culture with viruses or prions, particularly in medical biotechnology applications. Current practice is to minimize or eliminate the use of these ingredients wherever possible and use chemically defined media, but this cannot always be accomplished. Alternative strategies involve sourcing the animal blood from countries with minimum BSE/TSE risk, such as Australia and New Zealand, and using purified nutrient concentrates derived from serum in place of whole animal serum for cell culture.[2] Plating density (number of cells per volume of culture medium) plays a critical role for some cell types. For example, a lower plating density makes granulosa cells exhibit estrogen production, while a higher plating density makes them appear as progesterone-producing theca lutein cells.[3] Cells can be grown either in suspension or adherent cultures. Some cells naturally live in suspension, without being attached to a surface, such as cells that exist in the bloodstream. There are also cell lines that have been modified to be able to survive in suspension cultures so they can be grown to a higher density than adherent conditions would allow. Adherent cells require a surface, such as tissue culture plastic or microcarrier, which may be coated with extracellular matrix components to increase adhesion properties and provide other signals needed for growth and differentiation. Most cells derived from solid tissues are adherent. Another type of adherent culture is organotypic culture, which involves growing cells in a three-dimensional (3-D) environment as opposed to two-dimensional culture dishes. This 3D culture system is biochemically and physiologically more similar to in vivo tissue, but is technically challenging to maintain because of many factors (e.g. diffusion).ball

[edit] Cell line cross-contamination

Cell line cross-contamination can be a problem for scientists working with cultured cells. Studies suggest anywhere from 1520% of the time, cells used in experiments have been misidentified or contaminated with another cell line.[4][5][6] Problems with cell line crosscontamination have even been detected in lines from the NCI-60 panel, which are used routinely for drug-screening studies.[7][8] Major cell line repositories, including the American Type Culture Collection (ATCC) and the German Collection of Microorganisms and Cell Cultures (DSMZ), have received cell line submissions from researchers that were misidentified by them.[7][9] Such contamination poses a problem for the quality of research produced using cell culture lines, and the major repositories are now authenticating all cell line submissions.[10] ATCC uses short tandem repeat (STR) DNA fingerprinting to authenticate its cell lines.[11] To address this problem of cell line cross-contamination, researchers are encouraged to authenticate their cell lines at an early passage to establish the identity of the cell line. Authentication should be repeated before freezing cell line stocks, every two months during active culturing and before any publication of research data generated using the cell lines. Many methods are used to identify cell lines, including isoenzyme analysis, human lymphocyte antigen (HLA) typing, chromosomal analysis, karyotyping, morphology and STR analysis.[11] One significant cell-line cross contaminant is the immortal HeLa cell line.

[edit] Manipulation of cultured cells

As cells generally continue to divide in culture, they generally grow to fill the available area or volume. This can generate several issues:

Nutrient depletion in the growth media Accumulation of apoptotic/necrotic (dead) cells Cell-to-cell contact can stimulate cell cycle arrest, causing cells to stop dividing, known as contact inhibition. Cell-to-cell contact can stimulate cellular differentiation.

Among the common manipulations carried out on culture cells are media changes, passaging cells, and transfecting cells. These are generally performed using tissue culture methods that rely on sterile technique. Sterile technique aims to avoid contamination with bacteria, yeast, or other cell lines. Manipulations are typically carried out in a biosafety hood or laminar flow cabinet to exclude contaminating micro-organisms. Antibiotics (e.g. penicillin and streptomycin) and antifungals (e.g.amphotericin B) can also be added to the growth media. As cells undergo metabolic processes, acid is produced and the pH decreases. Often, a pH indicator is added to the medium to measure nutrient depletion. [edit] Media changes In the case of adherent cultures, the media can be removed directly by aspiration, and then is replaced. [edit] Passaging cells Main article: Passaging Passaging (also known as subculture or splitting cells) involves transferring a small number of cells into a new vessel. Cells can be cultured for a longer time if they are split regularly, as it avoids the senescence associated with prolonged high cell density. Suspension cultures are easily passaged with a small amount of culture containing a few cells diluted in a larger volume of fresh media. For adherent cultures, cells first need to be detached; this is commonly done with a mixture of trypsin-EDTA; however, other enzyme mixes are now available for this purpose. A small number of detached cells can then be used to seed a new culture. [edit] Transfection and transduction Main article: Transfection Main article: Transformation (genetics) Another common method for manipulating cells involves the introduction of foreign DNA by transfection. This is often performed to cause cells to express a protein of interest. More recently, the transfection of RNAi constructs have been realized as a convenient mechanism for suppressing the expression of a particular gene/protein. DNA can also be inserted into cells using viruses, in methods referred to as transduction, infection or transformation. Viruses, as parasitic agents, are well suited to introducing DNA into cells, as this is a part of their normal course of reproduction.

[edit] Established human cell lines

Cultured HeLa cells have been stained with Hoechst turning their nuclei blue, and are one of the earliest human cell lines descended from Henrietta Lacks, who died of cervical cancer from which these cells originated. Cell lines that originate with humans have been somewhat controversial in bioethics, as they may outlive their parent organism and later be used in the discovery of lucrative medical treatments. In the pioneering decision in this area, the Supreme Court of California held in Moore v. Regents of the University of California that human patients have no property rights in cell lines derived from organs removed with their consent.[12]

[edit] Generation of hybridomas

For more details on this topic, see Hybridoma. It is possible to fuse normal cells with an immortalised cell line. This method is used to produce monoclonal antibodies. In brief, lymphocytes isolated from the spleen (or possibly blood) of an immunised animal are combined with an immortal myeloma cell line (B cell lineage) to produce a hybridoma which has the antibody specificity of the primary lymphoctye and the immortality of the myeloma. Selective growth medium (HA or HAT) is used to select against unfused myeloma cells; primary lymphoctyes die quickly in culture and only the fused cells survive. These are screened for production of the required antibody, generally in pools to start with and then after single cloning.

[edit] Applications of cell culture

Mass culture of animal cell lines is fundamental to the manufacture of viral vaccines and other products of biotechnology Biological products produced by recombinant DNA (rDNA) technology in animal cell cultures include enzymes, synthetic hormones, immunobiologicals (monoclonal antibodies, interleukins, lymphokines), and anticancer agents. Although many simpler proteins can be produced using rDNA in bacterial cultures, more complex proteins that are glycosylated (carbohydrate-modified) currently must be made in animal cells. An important example of such a complex protein is the hormone erythropoietin. The cost of growing mammalian cell cultures is high, so research is underway to produce such complex proteins in insect cells or in higher plants, use of single embryonic cell and somatic embryos as a source for direct gene transfer via particle bombardment, transit gene expression and confocal microscopy

observation is one of its applications. It also offers to confirm single cell origin of somatic embryos and the asymmetry of the first cell division, which starts the process.

[edit] Cell culture in two dimensions

Research in tissue engineering, stem cells and molecular biology primarily involves cultures of cells on flat plastic dishes. This technique is known as two-dimensional (2D) cell culture, and was first developed by Wilhelm Roux who, in 1885, removed a portion of the medullary plate of an embryonic chicken and maintained it in warm saline for several days on a flat glass plate. With the advance of polymer technology, today's standard plastic dish for 2D cell culture, commonly known as the Petri dish, was invented by Julius Richard Petri, a German bacteriologist, who is generally credited with inventing the Petri dish while working as assistant to Robert Koch.

[edit] Tissue culture and engineering

Cell culture is a fundamental component of tissue culture and tissue engineering, as it establishes the basics of growing and maintaining cells ex vivo. The major application of human cell culture is in stem cell industry, where mesenchymal stem cells can be cultured and cryopreserved for future use.

[edit] Vaccines
Vaccines for polio, measles, mumps, rubella, and chickenpox are currently made in cell cultures. Due to the H5N1 pandemic threat, research into using cell culture for influenza vaccines is being funded by the United States government. Novel ideas in the field include recombinant DNA-based vaccines, such as one made using human adenovirus (a common cold virus) as a vector,[13][14] and novel adjuvants.[15]

[edit] Culture of non-mammalian cells

[edit] Plant cell culture methods
Main article: Plant tissue culture See also: Tobacco BY-2 cells Plant cell cultures are typically grown as cell suspension cultures in a liquid medium or as callus cultures on a solid medium. The culturing of undifferentiated plant cells and calli requires the proper balance of the plant growth hormones auxin and cytokinin.

[edit] Bacterial and yeast culture methods

Main article: Microbiological culture For bacteria and yeasts, small quantities of cells are usually grown on a solid support that contains nutrients embedded in it, usually a gel such as agar, while large-scale cultures are grown with the cells suspended in a nutrient broth.

[edit] Viral culture methods

Main article: Viral culture The culture of viruses requires the culture of cells of mammalian, plant, fungal or bacterial origin as hosts for the growth and replication of the virus. Whole wild type viruses, recombinant viruses or viral products may be generated in cell types other than their natural hosts under the right conditions. Depending on the species of the virus, infection and viral replication may result in host cell lysis and formation of a viral plaque.

[edit] Common cell lines

Human cell lines

HeLa National Cancer Institute's 60 cancer cell lines ESTDAB database DU145 (prostate cancer) Lncap (prostate cancer) MCF-7 (breast cancer) MDA-MB-438 (breast cancer) PC3 (prostate cancer) T47D (breast cancer) THP-1 (acute myeloid leukemia) U87 (glioblastoma) SHSY5Y Human neuroblastoma cells, cloned from a myeloma Saos-2 cells (bone cancer)

Primate cell lines

Vero (African green monkey Chlorocebus kidney epithelial cell line initiated in 1962)

Rat tumor cell lines

GH3 (pituitary tumor) PC12 (pheochromocytoma)

Mouse cell lines

MC3T3 (embryonic calvarium)

Plant cell lines

Tobacco BY-2 cells (kept as cell suspension culture, they are model system of plant cell)

Other species cell lines

Zebrafish ZF4 and AB9 cells Madin-Darby canine kidney (MDCK) epithelial cell line Xenopus A6 kidney epithelial cells

[edit] List of cell lines

This list is incomplete; you can help by expanding it. Cell line Meaning Organism Origin tissue Morphology Kidney (embryonic) Link Derivative of HEK 293 ECACC Cell Lines Data Base (CLDB) Also known as NIH 3T3 ECACC. Search for the many 3T3 cells in the CLDB.

293-T

Human

3T3 cells

"3-day transfer, inoculum 3 Mouse x 10^5 cells" Human Rat Human Human Human Human Murine Human Human Human Murine Murine Rat Human Bronchial epithelium + Adenovirus Human 12-SV40 virus hybrid

Embryonic fibroblast

721 9L A2780 A2780ADR A2780cis A172 A20 A253 A431 A-549 ALC B16 B35 BCP-1 cells

Melanoma Glioblastoma Ovary Ovary Ovarian cancer ECACC, CLDB

AdriamycinECACC resistant derivative Cisplatin-resistant Ovary ECACC derivative Glioblastoma Malignant glioma ECACC B lymphoma B lymphocyte Head and neck Submandibular duct carcinoma Skin Squamous cell ECACCCLDB epithelium carcinoma Lungcarcinom Epithelium DSMZECACC a Bone marrow Stroma PubMed Melanoma ECCAC Neuroblastom ATCC a PBMC HIV+ lymphoma ATCC

BEAS-2B

Lung

Epithelial

ATCC

bEnd.3

BHK-21

BR 293

BxPC3

(Ad12SV40) Brain Mouse endothelial Baby hamster kidney Hamster fibroblast cells Human Biopsy xenograph of Human pancreatic carcinoma line 3 Mouse Mouse Asian tiger mosquito Human Chinese hamster ovary Hamster Human Human Human Human Cercopithec us aethiops, origindefective SV-40

Brain/cerebral Endothelium cortex

ATCC

Kidney

Fibroblast

ECACCOlymp us

Breast

Breast cancer

Pancreatic adenocarcino Epithelial ma Myoblast cell line Embryonic mesenchymal cell line Larval tissue Tongue Ovary Lung Lung Lung Lung Epithelial Squamous cell carcinoma Epithelium

ATCC

C2C12 C3H-10T1/2 C6/36 Cal-27 CHO COR-L23 CORL23/CPR CORL23/5010 CORL23/R23

ECACC ECACC ECACC

ECACCICLC ECACC ECACC ECACC ECACC

COS-7

Ape Cercopithec us aethiops Kidney (Chlorocebu s) Human Ovary

Fibroblast

ECACCATCC

COV-434

Metastatic granulosa cell carcinoma T cell leukaemia

[1]ECACC

CML T1

Chronic myeloid Human leukaemia T lymphocyte

CML acute phase

Blood

CMT CT26 D17 DH82

1 Canine mammary tumor

Dog Murine Canine Canine

Mammary gland

Epithelium

Colorectal Colon carcinoma Osteosarcoma Histiocytosis Androgen insensitive carcinoma Metastatic prostate cancer Monocyte/macroph age Prostate

ECACC ECACC J Vir Meth

DU145

Human

PubMed DuCaP Dura mater cancer of the Human prostate Mouse Human Human Mouse Mouse Human Human Human CML blast crisis CML blast crisis Breast Breast Metastatic lymph node Lung Lung Epithelial {Ehrlich ascites carcinoma} mice ECACC CLDB CLDB ECACC ECACC

EL4 EM2 EM3 EMT6/AR1 EMT6/AR10 .0 FM3 H1299 H69 HB54 HB55

T cell leukaemia Ph+ CML line Ph+ CML line Epithelial-like Epithelial-like Melanoma Lung cancer

Hybridoma Hybridoma Hybridoma Hybridoma

HCA2 Human embryonic kidney "Henrietta Lacks" Clone 7 of

Human

Fibroblast Kidney (embryonic) Cervical cancer Hepatoma

ECACC Secretes L243 mAb Human (against HLA-DR) Immunology secretes MA2.1 Journal of mAb (against HLAImmunology A2 and HLA-B17) Journal of General Virology Epithelium Epithelium Epithelial ATCC DSMZECACC ECACC

HEK-293 HeLa Hepa1c1c7

Human Human Mouse

HL-60

HMEC

clone 1 hepatoma line 1 Human leukemia Human mammary epithelial cell

ATCC Human Myeloblast Blood cells ECACCDSMZ

Human

Epithelium

ECACC ECACC

HT-29

Human

Colon epithelium T cell leukemia

Adenocarcinoma CLDB ECACC white blood cells DSMZ

Jurkat JY cells K562 cells Ku812 KCL22 KG1 KYO1 LNCap Ma-Mel 1, 2, 3....48 MC-38 MCF-7 Michigan Cancer Foundation7 Michigan Cancer Foundation M.D. Anderson metastatic breast M.D. Kyoto 1

Human Human Human Human Human Human Human

Lymph node cancer of the Human prostate Human Mouse Human

Lymphoblasto EBV immortalised id B cell Lymphoblasto CML blast crisis ECACC id ECACC Lymphoblasto Erythroleukemia id LGCstandards Lymphoblasto CML id Lymphoblasto AML id Lymphoblasto CML DSMZ id Prostatic adenocarcino Epithelial ECACCATCC ma A range of melanoma cell lines Adenocarcinoma Mammary gland Mammary gland Invasive breast ductal carcinoma ER+, PR+

MCF-10A

Human

Epithelium

ATCC

MDA-MB231 MDA-MB-

Human Human

Breast Breast

Cancer Cancer

ECACC ECACC

468

MDA-MB435

MDCK II

MDCK II MG63 MOR/0.2R MONOMAC 6 MRC5 MTD-1A MyEnd NCIH69/CPR NCIH69/LX10 NCIH69/LX20 NCIH69/LX4 NIH-3T3

Anderson metastatic breast M.D. Anderson Human Metastatic Breast Madin Darby Dog canine kidney Madin Darby Dog canine kidney Human Human Human Human (foetal) Mouse Myocardial Mouse endothelial Human Human Human Human NIH, 3-day transfer, Mouse inoculum 3 5 x 10 cells

Breast

Melanoma or carcinoma (disputed)

Cambridge Pathology ECACC

Kidney

Epithelium

ECACC ATCC

Kidney Bone Lung WBC Lung

Epithelium Osteosarcoma

[2] ATCC

ECACC Myeloid metaplasic CLDB AML Fibroblast] Epithelium Endothelium

Lung Lung Lung Lung

ECACC ECACC ECACC ECACC

Embryo

Fibroblast

ECACCATCC Cancer Genetics and Cytogenetics ESTDAB Asterand DSMZ

NALM-1 NW-145 OPCN / OPCT cell lines Peer PNT-1A /

Peripheral blood Onyvax [3] prostate cancer.... Human T cell leukemia

Blast-crisis CML Melanoma Range of prostate tumour lines

Prostate tumour

ECACC

PNT 2 RenCa RIN-5F RMA/RMA S Saos-2 cells Sf-9 Renal carcinoma Mouse Mouse Mouse Human Insect Spodoptera Spodoptera Ovary frugiperda frugiperda (moth) Cervical Human cancer Human Human Human Mammary gland Colorectal carcinoma / Lung metastasis Monocyte Pancreas

lines Renal carcinoma

T cell tumour Osteosarcoma ECACC DSMZECACC

SiHa SkBr3 T2 T-47D

Epithelium

ECACC

Breast carcinoma T cell leukemia/B DSMZ cell line hybridoma Ductal carcinoma

T84 THP1 cell line U373 U87 U937 Vertebra prostate cancer

Human

Epithelium

ECACCATCC

Human Human Human Human

AML

ECACC

VCaP

Human

GlioblastomaEpithelium astrocytoma GlioblastomaEpithelial-like astrocytoma Leukaemic monocytic lymphoma Metastatic prostate Epithelial cancer Kidney epithelium

Abcam ECACC

ECACC ATCC

Vero cells WM39 WT-49 X63 YAC-1 YAR

African Vero (truth) green monkey Human Human Mouse Mouse Human

ECACC

Skin Primary melanoma Lymphoblasto id Melanoma Lymphoma CLDB ECACC [4] Human B cell EBV transofrmed Immunology

[edit] See also

Biological immortality Cell culture assays Electric cell-substrate impedance sensing List of contaminated cell lines Organ culture Plant tissue culture Tissue culture

[edit] References and notes

1. ^ {{cite web|url=http://caat.jhsph.edu/pubsthe virus in monkey kidney cell cultures. 2. ^ "LipiMAX purified lipoprotein solution from bovine serum". Selborne Biological Services. 2006. http://www.selbornebiological.com/products/lipimax.htm. Retrieved 2010-02-02. 3. ^ Portela VM, Zamberlam G, Price CA (April 2010). "Cell plating density alters the ratio of estrogenic to progestagenic enzyme gene expression in cultured granulosa cells". Fertil. Steril. 93 (6): 20505. doi:10.1016/j.fertnstert.2009.01.151. PMID 19324349. 4. ^ Drexler, HG; Dirks, WG; Macleod, RA (Oct 1999). "False human hematopoietic cell lines: cross-contaminations and misinterpretations". Leukemia 13 (10): 16017. doi:10.1038/sj/leu/2401510. ISSN 0887-6924. PMID 10516762. 5. ^ Drexler, HG; Macleod, RA; Dirks, WG (Dec 2001). "Cross-contamination: HSSultan is not a myeloma but a Burkitt lymphoma cell line" (Free full text). Blood 98 (12): 34956. doi:10.1182/blood.V98.12.3495. ISSN 0006-4971. PMID 11732505. http://www.bloodjournal.org/cgi/pmidlookup?view=long&pmid=11732505. 6. ^ Cabrera, CM; Cobo, F; Nieto, A; Corts, JL; Montes, RM; Catalina, P; Concha, A (Jun 2006). "Identity tests: determination of cell line cross-contamination". Cytotechnology 51 (2): 4550. doi:10.1007/s10616-006-9013-8. ISSN 0920-9069. PMID 19002894. 7. ^ a b Chatterjee, R (Feb 2007). "Cell biology. Cases of mistaken identity.". Science 315 (5814): 92831. doi:10.1126/science.315.5814.928. ISSN 0036-8075. PMID 17303729. 8. ^ Liscovitch, M; Ravid, D (Jan 2007). "A case study in misidentification of cancer cell lines: MCF-7/AdrR cells (re-designated NCI/ADR-RES) are derived from OVCAR-8 human ovarian carcinoma cells.". Cancer letters 245 (1-2): 3502. doi:10.1016/j.canlet.2006.01.013. ISSN 0304-3835. PMID 16504380. 9. ^ Macleod, RA; Dirks, WG; Matsuo, Y; Kaufmann, M; Milch, H; Drexler, HG (Nov 1999). "Widespread intraspecies cross-contamination of human tumor cell lines arising at source.". International journal of cancer. Journal international du cancer 83 (4): 55563. doi:10.1002/(SICI)1097-0215(19991112)83:4<555::AIDIJC19>3.0.CO;2-2. ISSN 0020-7136. PMID 10508494. 10. ^ Masters, JR (Apr 2002). "HeLa cells 50 years on: the good, the bad and the ugly.". Nature reviews. Cancer 2 (4): 3159. doi:10.1038/nrc775. ISSN 1474-175X. PMID 12001993. 11. ^ a b Dunham, J.H.; Guthmiller, P. (2008). "Doing good science: Authenticating cell line identity" (PDF). Cell Notes 22: 1517. http://www.promega.com/cnotes/cn022/cn022_15.pdf.

12. ^ Ceb.com 13. ^ Reuters (2006-01-26). Wired.com "Quickie Bird Flu Vaccine Created". Wired. http://wired.com/news/wireservice/0,70102-0.html?tw=wn_index_7 Wired.com. Retrieved 2010-01-31. 14. ^ Gao W, Soloff AC, Lu X, Montecalvo A, Nguyen DC, Matsuoka Y, Robbins PD, Swayne DE, Donis RO, Katz JM, Barratt-Boyes SM, Gambotto A. (February 2006). "Protection of mice and poultry from lethal H5N1 avian influenza virus through adenovirus-based immunization". Journal of Virology (United States: American Society for Microbiology) 80 (4): 19591964. doi:10.1128/JVI.80.4.1959-1964.2006. ISSN 0022-538X. PMC 1367171. PMID 16439551. http://jvi.asm.org/cgi/content/abstract/80/4/1959. Retrieved 2010-01-31. 15. ^ "NIAID Taps Chiron to Develop Vaccine Against H9N2 Avian Influenza". National Institute of Allergy and Infectious Diseases (NIAID). 2004-08-17. http://www3.niaid.nih.gov/news/newsreleases/2004/h9n2.htm. Retrieved 2010-0131.[dead link]

Hpacultures.org.uk, Health Protection Agency Culture Collections (ECACC) MacLeod, R. A. F.; Dirks, Wilhelm G.; Matsuo, Yoshinobu; Kaufmann, Maren; Milch, Herbert; Drexler, Hans G. (1999). "Widespread intraspecies crosscontamination of human tumour cell lines". International Journal of Cancer 83 (4): 555563. doi:10.1002/(SICI)1097-0215(19991112)83:4<555::AID-IJC19>3.0.CO;22. PMID 10508494. Masters, John R. (2002). "HeLa cells 50 years on: the good, the bad and the ugly". Nature Reviews Cancer 2 (4): 315319. doi:10.1038/nrc775. PMID 12001993. Recently invented was the 3D Petri dish, the first 3D cell culture offering.

[edit] External links

Helpful Hints for Better Aseptic Technique Information About Cell Culture Testing Table of common cell lines from Alberts 4th ed. Cancer Cells in Culture Hypertext version of the Cell Line Data Base Cell Culture Basics - Introduction to cell culture, covering topics such as laboratory set-up, safety and aseptic technique including basic cell culture protocols and video training Database of Who's Who in Cell Culture and Related Research Witkowski JA. Experimental pathology and the origins of tissue culture: Leo Loeb's contribution. Med Hist. 1983 July; 27(3): 269288. Coriell Cell Repositories The National Centre for Cell Science (NCCS), Pune, India; national repository for cell lines/hybridomas etc. Neural Stem Cell Culture: Neurosphere generation, microscopical analysis and cryopreservation (a protocol) Rat Chromaffin cells primary cultures: Standardization and quality assessment for single-cell assays (a protocol)

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