ALLA A. BONDAREVA, BS, MS, MA 101 McIntosh Ct., Apt.

A Bozeman, MT 59715 (406)587-9031 Objective: Research Associate in Molecular Biology

HIGHLIGHTS OF QUALIFICATION Two Masters degrees: M.S. degree in Biological Sciences with specialization in molecular biology and cytogenetics, and M.A. degree in Economics with specialization in business management Broadly trained and highly experienced molecular biologist with more than 10 years of professional research, teaching, managing, and technical experience Good working knowledge and experience in different methods and technologies in molecular genetics, including transgenic technologies, embryological procedures, immunohistology, in situ hybridization assays, cloning, protein expression and purification, single gene- and genome-based expression assays, microscopy, bioinformatics and phylogenetics Demonstrated expertise in studying complex gene regulation using molecular-, genetic-, genomic-, developmental-, and phylogenetic-based approaches Able to develop novel molecular, biochemical, histological, and other analytical/quantitative assays and technologies, experience in training others in these techniques Extensive experience in managing a molecular genetics research lab and supervising students, technicians, and assistants; management of personnel, materials, equipment, and funds in laboratories as well as business/industry setting Experience in directing and training of research personnel Demonstrated ability to conduct independent research and publish results in refereed journals Excellent analytical skills Highly creative and productive, competent and confident Strong responsible, and reliable Largely self-directed, confident, able to work both independently and as a team member Intellectual ability to identify and analyze the problem, and technical and scientific knowledge to solve all manners of questions, puzzles, and problems as they arise Contributed to the research field with every project scientific and industrial have been involved in EXPERIENCE AND RELEVANT SKILLS Experience in transgenic, and embryology techniques: Targeting vector design and production for knock-out and gene targeting in mice Gene targeting and screening in ES cells

Transgenic mouse production Embryo harvest and manipulation

Experience and lab skills in gene expression assays: Designing and synthesis of RNA probes for RNase protection assay and for ISH RNase Protection In situ hybridization (ISH) to RNA on frozen tissue sections and whole mount ISH Primer labeling for primer extension assay, and Primer Extension Northern blot SAGE

Experience and lab skills in molecular biology: RACE cloning Construction and screening of phage and plasmid DNA libraries Nuclei isolation and nuclear extract preparation Enzyme purification RNA isolation and purification Isolation of poly-A+ mRNA and synthesis of cDNA RT-PCR cloning Isolation and purification of phage, plasmid, and genomic DNA Mouse genotyping analysis, PCR-genotyping Gene amplification, PCR and RT-PCR Agarose and acrylamide gel electrophoresis, elution of DNA fragment from gel Automated DNA sequencing on ABI 310 and ABI 377 Designing and random primer labeling of DNA probes Southern blotting Kinase end-labeling of DNA Klenow end-blunting and end-labeling of DNA fragments Competent cell preparation Restriction mapping of DNA Recombinant DNA cloning

Experience and lab skills in immunochemistry: SDS-polyacrylamide gel electrophoresis Western blotting Radiolabeling of antibodies Labeling of antibodies by colloidal gold Immunoblot assay Radioimmunoassay Protein purification including affinity chromatography and gel filtration Biotinylation of proteins and oligopeptides

Experience and skills in microscopy: Dissection and tissue harvest Cryosectioning Purification of ultrastructural cell components and fixation of samples for electron microscopy (EM) Embedding in epoxy resins for EM Preparation of ultrathin cuts for EM Localization of antigens by means of EM and colloidal gold labeled antibodies or PrA

Experience in genetic and phylogenetic techniques: Genotyping Marker identification Sequence alignments Nonsynonymous / synonymous substitution rates estimation Phylogenetic tree construction Relatedness and common ancestor prediction Cladistics EDUCATION MS in Biology: molecular biology and cytogenetics (diploma with excellence) Tomsk State University. Tomsk, Russia 1992 (Degree equivalent to U.S. Masters Degree in Biology and Teaching of Biology and Chemistry at secondary level) MA in Economics: economics and business management Novosibirsk State University. Novosibirsk. Russia 1997 (Degree equivalent to U.S. Masters Degree in Economics and Business Management) EMPLOYMENT HISTORY 1999-present Research Associate/Lab Manager, Lab of Molecular Genetics and Development of Dr. Edward Schmidt, Department of Veterinary Molecular Biology, Montana State University, Bozeman, USA. Applied research: Using targeted mutagenesis in mice to study the changes in evolution of the basal transcription machinery and mechanisms of gene regulation, which control physiological processes that are unique to higher metazoan animals, such as regulation of immune system. Accomplishment: Gene expression, splicing fidelity and efficiency were demonstrated for numerous genes expressed in transgenic mice in comparing to wild type (mice) by different methods RNase protection, RT-PCR, Primer extension, In situ hybridization. The results of this research have led to several publications - in Cell, JBC, and MB&E. Acquired extensive experience in managing and supervising the research lab with

graduate students, technicians, and assistants; developed novel techniques for the lab and trained others 1996-1999 Economist, Mining company Pluton, Kemerovo, Russia Applied research: Data of hundreds of Russian and foreign mining companies were analyzed to estimate main factors that have influence on the profitability of gold mining enterprises. In collaboration with others worked out the Plan of Development of Gold Mining in Kemerovo Region for the years 1998-2010. Accomplishment: Theses of defense for Masters Degree Economic problems of gold mining in Russia were published and presented to the Government of Russia for consideration of changes in legislature on gold mining in Russia. Acquired extensive experience in managing of funds, equipment maintenance, supplies, human and material resources in the enterprise 1993-1996 Environmental Manager, Mining company Vulkan, Gorny Altay, Russia Applied research: Analyzed the best ways to protect environment at and after the development of gold deposits Accomplishment: The results of this research have led to the publication of 2volume edition bench book for environmental managers in mining companies. Acquired experience in managing the group of research technicians and assistants, including managing of funds, equipment, supplies, resources. Provided quality-control of mining activity of the enterprise and prepared reports to the controlling organizations 1992-1993 Teacher of Biology and Chemistry, Middle and High Scool Zhemchuzhnaya, Shira, Krasnoyarsky Kray, Russia Accomplishment: Acquired extensive experience in teaching students of biology and chemistry 1986-1992 Head Lab Technician/Research Associate, Lab of Molecular Cytogenetics of Dr. Igor Zhymulov, Institute of Cytology and Genetics, Novosibirsk, Russia Applied research: Using immunocytochemical methods and electron microscopy to study localization and function of surface antigens of brain cortex synaptosomes. Used combined behavioral cellular, and molecular biological approaches to delineate the changes that underline simple forms of learning and memory. Accomplishment: Developed a new method of antigen identification on the reaction of immunoblot-strips by binding to colloidal gold-labeled antibodies. Worked out new procedures for preparing of colloidal gold and binding colloidal gold to antibodies that result in significant increasing of stability of Au-antibody complex. Stability of this complex becomes higher than ones for similar complexes prepared in other laboratories and companies. Prepared by this procedure colloidal gold-antibody complexes were delivered and successfully used in many research laboratories of Russia (Moscow, St.-Peterburg, Novosibirsk, Irkutsk) and Lytva (Vilnus). Worked out in collaboration with others new procedure for the significant decreasing of nonspecific interactions at the affinity chromatography and at the determination of protein localization by electron microscopy. Co-author on one publication resulting from this research. 1984-1986 Lab Technician, Lab of Fetoplacental Pathology of Dr. Nikolai Cyrelnikov, Institute of Clinical and Experimental Medicine, Novosibirsk, Russia

1982-1984 Lab Technician, Lab of Genetic Engineering of Dr. Alexander Grachov, Institute of Cytology and Genetics, Novosibirsk, Russia Accomplishment: Developed a novel assay which enables to determine antigen quantity in isolated cellular structures by means of affinity chromatography and spot-analysis 1981-1982 Lab Technician, Lab of Population Biology of Dr. Sergey Ivanov, Institute of Biology, Novosibirsk, Russia PUBLICATIONS Bondareva, A.A. and E.E. Schmidt (2003). Early vertebrate evolution of the TATA-binding protein, TBP. Mol.Biol. Evol, 20 (11), 1932-1939 Schmidt, E.E., A.A. Bondareva, J.R. Radke, and M.R. Capecchi (2003). Fundamental cellular processes do not require vertebrate-specific sequences within the TATA-binding protein. J.Biol.Chem., 278 (8), 6168-6174 Hobbs, N.K., A.A. Bondareva, S. Barnett, M.R. Capecchi, and E.E. Schmidt (2002). Removing the vertebrate-specific TBP N terminus disrupts placental 2m-dependent interactions with the maternal immune system. Cell, 101 (1), 43-54 Protection of Environment under the development of gold deposits, edited by Nikolaenkova A.A. (Bondareva A.A.) volumes 1, 2. Novosibirsk, Nauka, Russia, 1995, 257p. (in Russian) Starostina, M.V., A.A. Nikolaenkova (A.A. Bondareva), T.K. Malup, L.I. Korochkin, S.M. Sviridov (1993). Quantitative assay of S-100 protein in mouse brain cortex synaptosomes. Cellular and Molecular Neurobiology, 13 (6), 677-691 OTHER PUBLISHED ACKNOWLEDGEMENTS Technical contribution acknowledged in Schmidt, E.E., D.S. Taylor, J.R. Prigge, S. Barnett, and M.R. Capecchi (2000). Illegitimate Cre-dependent chromosome rearrangements in transgenic mouse spermatids. Proc. Natl. Acad. Sci. USA 97 (5), 13702-13707

References: Dr. Edward Schmidt, Assistant Professor, Veterinary Molecular Biology, Montana State University, Bozeman (406) 994-6375 Dr. Michele Hardy, Assistant Professor, Veterinary Molecular Biology, Montana State University, Bozeman (406) 994-6378 Nikolai Kholodilov, Associate Research Scientist, Department of Neurology, Columbia University, New York, USA (212) 305-1540 Eduard Dubrovsky, Research Assistant Professor, Department of Biology, Dartmouth College, Hanover, USA (603) 646-2142 Andrey Rzhetsky, Assistant Professor, Faculty of Biomedical Informatics, Columbia University, New York (212) 304-7552