BLOOD CELL OBSERVATION Purpose

Watching the red blood cell and white blood cell

Based theory
A blood cell, also Called a hematocyte, is a cell normally found in blood . In Mammals , these fall

into three general categories:

Red blood cells - erythrocytes White blood cells - leukocytes Platelets - Thrombocytes

Together, these three Kinds of blood cells add up to a total of 45% of the blood tissue by volume, with the remaining 55% of the volume composed of plasma , the liquid component of blood. This volume percentage (eg, 45 %) of cells to the total volume is Called hematocrit , determined by centrifuge or flow cytometry . Hemoglobin (the main component of red blood cells) is an iron -containing proteins the which facilitates transportation of oxygen and otherrespiratory gases to tissues. Red Blood Cells (Erythrocytes) Red blood cells are primarily for carrying oxygen and some carbon dioxide through the use of hemoglobin and have a lifetime of about 120 days. In the process of being formed They go through being a stem cell, a stem cell monopotent, a Proerythroblast, Erythroblast, reticulocyte, and then Becomes a carrying red blood cell glucose. These cells are incomplete. They do not contain a nucleus. White blood cells ( leukocytes) White blood cells, or leukocytes (also spelled "leucocytes", leuco-Ancient Greek "white"), are cells of the immune system in defending the body INVOLVED Both against infectious disease and foreign materials. Five different and diverse types of leukocytes exist, but They are all produced and derived from a multipotent cell in the bone marrow known as a hematopoietic stem cell. They live for about 3 to 4 days in the average human body. Leukocytes are found throughout the body, including the blood and lymphatic system. Platelets (Thrombocytes) Platelets, or thrombocytes, are small, irregularly shaped cell fragments clear, 2-3 m in diameter, the which are derived from fragmentation of precursor megakaryocytes. The average lifespan of a platelet is normally just 5 to 9 days. Platelets are a natural source of growth factors. They Circulate in the blood of Mammals and are INVOLVED in hemostasis, leading to the formation of blood clots. Platelets release thread-like fibers to form these clots.

Tools and material

1. Objects Glass

2. Cover Glass

3. Microscope

4. Pipette

5. Methylene blue

6. Alcohol 70%

7. Cotton

8. Blotting paper/ tissue

9. Toothpick

10.Blood Lancet

Procedure
1. Damping cotton with 70% alcohol, then wipe it to the tip of a finger 2. Sticking the fingertip using blood lancet 3. Putting the blood on the glass objects and then uses as drops with a litle methylene blue. When methylene blue excess, blotting with a tissue/blotting paper and flatten with a toothpick 4. Closing blood with cover glass, and then observe under microscope 5. Creating reports result of an experiment you have done

Observation result

White Blood cells

Red Blood Cells

Note : the one which absorb metylene blue is white blood cells, and the spots around white blood cells are red blood cells.

Conclusion
In the blood cells there are contain white blood cells, red blood cells, and platelets. And the white blood cells there contain granulosit so it can absorb methylene blue, but in the red blood there are no granulosit so it cannot absorb methylene blue. From the observation result it can show that eritrosit have larger population than leukosit.