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Cell Culture Dishes

Cell Culture Treated Dishes
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Corning CellBIND Surface is a novel cell culture treatment that increases surface wettability for more even and consistent cell attachment Ultra-Low Attachment Surface dishes feature a covalently bound hydrogel layer that minimizes cell attachment, protein absorption and cellular activation 6-pack carriers with only 10 dishes/bag are available for 100 mm dishes (Cat No. 430293) 245 mm square dishes offer 500cm2 growth surface Manufactured from optically clear virgin polystyrene Sterilized by gamma radiation Certified nonpyrogenic Stacking beads aid in handling Vents provide consistent gas exchange

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3296 100 mm Dishes coated with Corning CellBIND Surface

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Corning Cell Culture Dish Ordering Information

Cat. No. Surface Dish Style* (mm) Approx. Height (mm) Growth Area (cm2) Qty/Pk Qty/Cs

3294
430196 Gridded 60 mm Dish

430165 430166 3295 3261 3262 430196

Corning CellBIND Surface TC TC Corning CellBIND Surface Ultra-Low Attachment Ultra-Low Attachment TC Corning CellBIND Surface TC TC TC TC

35 35 60 60 60 100 60 with 2 mm grid 100 100 100 150 245

10 10 15 15 15 20 15 20 20 20 25 25

8 8 21 21 21 55 21 55 55 55 148 500

10 20 20 7 5 5 20 5 20 10 5 4

210 500 500 126 20 20 500 40 500 480 60 16

431110 500 cm2 Cell Culture Dish

3296 430167 430293 430599 431110

Cell Culture Tip

Check the Corning web site (www.corning. com/lifesciences) for technical cell culture application bulletins.

*Dish style (mm) = actual growth surface diameters: 35 mm dish = 34.4 mm; 60 mm dish = 52.1 mm; 100 mm dish = 83.8 mm; 150 mm dish = 139.1 mm. The square dishes have interior bottom dimensions of 224 mm x 224 mm.

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Quantum Scientific - a VWR Company Crown Scientific - a VWR Company Visit www.corning.com/lifesciences for additional product and technical information. VWR International, Pty Ltd. Freecall: 1800 777 168 Freefax: 1800 625 547 sales@quantum-scientific.com.au www.quantum-scientific.com.au

VWR International, Pty Ltd. Freecall: 1300 727 696 Freefax: 1300 135 123 sales@crownsci.com.au www.crownscientific.com.au

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Corning Not Treated Cell Culture Dishes

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Manufactured from optically clear virgin polystyrene Not cell culture treated for applications where cell attachment is not desired Stacking beads aid in handling Vents provide consistent gas exchange Sterilized by gamma radiation Certified nonpyrogenic

Cell Culture Dish Application Tips

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The 150 and 245 mm culture dishes make excellent carriers and incubator trays for 35 and 60 mm dishes. This helps prevent spills and reduces opportunities for contamination. Corning recommends 0.2 to 0.3 mL of medium per cm2 of growth area.

Corning Nontreated Cell Culture Dish Ordering Information

Cat. No. Dish Style* (mm) Height (mm) Approx. Growth Area (cm2) Qty/Pk Qty/Cs

430588 430589 430591 430597 431111

35 60 100 150 245

10 15 20 25 25

9 21 55 152 500

20 20 20 5 4

500 500 500 60 16

*Note: Dish style (mm) = actual growth surface diameters: 35 mm dish = 34.4 mm; 60 mm dish = 52.1 mm; 100 mm dish = 83.8 mm; 150 mm dish = 139.1 mm. Cat. No. 431111 is a square dish with interior bottom plate dimensions of 224 mm x 224 mm.

Expected Cell Yields and Recommended Medium Volumes

Corning Dishes Approximate Growth Area (cm2) Average Cell Yield* Recommended Medium Volume (mL)

35 mm 60 mm 100 mm 150 mm 245 mm (square)

9 21 55 152 500

9.0 x 105 2.1 x 106 5.5 x 106 1.52 x 107 5.0 x 107

1.8 - 2.7 4.2 - 6.3 11 - 16.5 30.4 - 45.6 100 - 150

*Assumes an average yield of 1 x 105 cells/cm2 from a 100% confluent culture. Yields from many cell types can be lower than this.

Costar Center Well Culture Dish

Product is strictly not for human use. The center well dish is a Class I medical device and should never be used in applications which require the product to be certified as a Class II or Class III medical device. This product is not CE marked.
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20 mm center well Inner well holds 3 mL of medium, while the outer well holds 10 mL Treated for optimal cell attachment Sterilized by gamma radiation Certified nonpyrogenic For research use only
Outer moat for humidification and washing 45 slope funnels cells to center well See it all Entire focal plane is visible Stand-off rim on base protects optical surface from scratching Edge beads permit uniform gas flow 1 mL level is convenient for dispensing media. Fill to bottom of bevel for 1 mL Center well isolates cells for easier access Beveled lead-in makes it easier to manipulate pipette

3260 Center Well Culture Dish

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Costar Center Well Culture Dish Ordering Information

Cat. No. Size (mm) Description (mm) Center Well (mm) Qty/Pk Qty/Cs

3260

60

60 x 15

20

20

500

2 Quantum Scientific - a VWR Company VWR International, Pty Ltd. Freecall: 1800 777 168 Freefax: 1800 625 547 sales@quantum-scientific.com.au www.quantum-scientific.com.au Crown Scientific - a VWR Company 17 VWR International, Pty Ltd. Freecall: 1300 727 696 Freefax: 1300 135 123 sales@crownsci.com.au www.crownscientific.com.au