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Administrative Details
Instructor: Assistant Professor Yong Woo Cho, PhD Office: Room # 38-411, The 4th floor, Engineering Building V E-mail: ywcho7@hanyang.ac.kr Tel: (OP) 031-400-5279, (CP) 010-4052-1012 TA: Hwa In Yoon, E-mail: fineyoon@gmail.com, (OP) 4712, (CP) 010-4744-6321 Homepage: http://www.biomedpolym.re.kr Lecture: Monday 4:30 6:00 PM & Tuesday 4:00 5:30 PM Text: Bioprocess Engineering Basic Concepts 2nd Edition (by M. L. Shuler and F. Kargi) 1 Midterm (40%), 1 Final (40%), and Assignments (20%).
Course Outline
Part I. Introduction Chapter 01. What is a Bioprocess Engineer? Part II. The Basics of Biology: An Engineers Perspective Chapter 02. An Overview of Biological Basics Chapter 03. Enzymes Chapter 04. How Cells Work Chapter 05. Major Metabolic Pathways Chapter 06. How Cells Grow Chapter 07. Stoichiometry of Microbial Growth and Product Formation Chapter 08. How Cellular Information Is Altered Midterm Exam Part III. Engineering Principles for Bioprocesses Chapter 09. Bioreactors Chapter 10. Scale-Up and Control of Bioreactors Chapter 11. Recovery and Purification of Products Part IV. Applications to Nonconventional Biological Systems Chapter 15. Tissue Engineering and Gene Therapy Final Exam
We will focus primarily on the application of chemical engineering principles to systems containing biological catalysts, but with an emphasis on those systems making use of biotechnology
1.4. The Story of Penicillin: How Biologists and Engineers Work Together
Antibiotics are one of the great marvels of modern medicine
Figure. The mold produces penicillin. The first antibiotic discovered was penicillin, which is made by the common mold Penicillium notatum, shown here growing on bread and in a close-up view. Alexander Fleming discovered penicillin in 1928. He was trying to grow bacteria (Staphylococus aureus), but the pesky mold Penicillium notatum had contaminated his bacterial cultures. Fleming noticed that bacteria did not grow near the mold. Fortunately, he recognized the value of an agent that could inhibit bacterial growth, and the age of antibiotic was born.
Staphylococus Bacteria
Figure. Fungal production of an antibotic. The mold Penicillium produces an antibiotic that inhibits the growth of Staphylococcus bacteria, resulting in the clear area between the mold and the bacteria
Staphylococus Bacteria
They cause boils
Staphylococcus bacteria cling to hairlike appendages called cilia on human skin cells
Multidisciplinary Work
This accomplishment required a high level of multidisciplinary work Merck realized that men who understood both engineering and biology were not available. Merck assigned a chemical engineer and microbiologist together to each aspect of the problem. They planned, executed, and analyzed the experimental program jointly, almost as if they were one man Progress has involved better understanding of mold physiology, metabolic pathways, penicillin structure, methods of mutation and selection of mold genetics, process control, and reactor design.
GMP Concerns
The actual manufacturing facility design and layout The equipment and procedures Training of production personnel Control of process inputs (e.g., raw materials and cultures) Handling of products Prevent contamination Dictate flow of material, personnel, and air Procedure validation: include not only operation of equipments but also cleaning and sterilization Key concepts: Written documentation, consistency of procedures, consistency of product, and demonstrable measures of product quality (particularly purity and safety)