Hematology II: Lecture and Student Laboratory Schedule CLS 322, Spring, 2009 Course Coordinator: Thomas B.

Wiggers, M.S., SH(ASCP) Associate Professor Clinical Laboratory Sciences

cm

Guest Faculty:

Renee Wilkins, MS, MLS(ASCP)cm Instructor, Program In Clinical Laboratory Sciences UMMC

REQUIRED TEXTBOOK: Lecture & Laboratory Clinical Laboratory Hematology, Shirlyn B. Mckenzie PhD, Prentiss Hall, 2004, ISBN: 0-13-019996-6 Chapters covered in this course: 10, 11, 12, 13, 14, 16, 17, 18, 19, 20, 21, 22, 26, 27, 29, 30, 34, 35, 36, 37, 38, 39, 41

DATE January 15

HOUR

TOPIC

Week 1: Introduction to the Anemias Hematology Lect RBC Disorders-Anemia: Classification, Etiology Hematology Lect RBC Disorders-Anemia: Classification, Morphology Hematology Lab Normal Differential Review Reading Assignment: Chapter 10

Course Syllabus and Specific Objectives [syl322.doc] Course Objectives CLS 312 and CLS 322 [objectives.doc] Technical Curriculum Report 1 [tcrpt1.pdf] Course objectives derived from the recommendations of the ASCP Board of Registry Technical Curricula document. All letter references are to the Knowledge/Ability criteria listed in those documents which are found at: http://www.ascp.org/bor/directors/tech_mt/ Following the completion of CLS 311 (Immunology), CLS 312 (Hematology), and CLS 322 (Clinical Hematology), as applicable, the student will be able to accomplish each of the objectives outlined in this report. Technical Curriculum Report 2 [tcrpt2.pdf] Course objectives derived from the recommendations of the ASCP Board of Registry Technical Curricula document. All letter references are to the Knowledge/Ability criteria listed in those documents which are found at: http://www.ascp.org/bor/directors/tech_mt/ . This report contains the same information found in Report 1, presented in an alternate format. Following the completion of CLS 311 (Immunology), CLS 312 (Hematology), and CLS 322 (Clinical Hematology), as applicable, the student will be able to accomplish each of the objectives outlined in this report. Course Cross Reference [ccr.pdf] This report shows where each of the items listed in the Technical Curricula reports is presented in the two year CLS curriculum. It lists the specific handouts, clinical rotations, courses, etc., where each objective is covered. Course Schedule [322scd08.doc] Glossary of Hematology [hemaglos.doc] Red Cell Morphology Glossary [rbcgloss.doc] Red Cell Morphology Overview [rbcmorph.doc] CLS 322 Pre-Test (Obtain password from instructor to take this test.) ----------------------

January 22

Week 2: RBC Indices / Anemias, General Hematology Lect Red Blood Cell Indices & Correlation w/Smear Morphology Hematology Lect Normochromic, Normocytic Anemia Hematology Lab Reticulocyte Counts Review/ESR Reading Assignment: Chapter 10
Performance of a Normal Slide Differential [normdiff.doc] CAP Guidelines Band vs. Seg [cap_band_seg.doc]

Criteria for Manual Review of Differentials UMMC [revcrit.doc] Red Cell Indices [indices.doc The Reticulocyte Count and Sedimentation Rate [reticesr.doc] Embryogenesis of Blood & Bone Marrow [embryogn.doc] General Overview of Anemia [anemias.doc] Laboratory Analysis of Anemias [anemlab.doc] Lab Analysis of Anemias Flowchart [anemia flowchart.pdf]

January 29

Week 3: RBC Disorders-Hypochromic, Microcytic Anemia Hematology Lect Hypochromic, Microcytic Anemia Hematology Lect Hypochromic, Microcytic Anemia Hematology Lab Abnormal Differentials: Anemias Reading Assignment: Chapter 11
Slide Set #1 Abnormal RBC and Anemia [abnrbcsl.doc]

February 5

Week 4: RBC Disorders- Megaloblastic Anemia Hematology Lect Macrocytic/Megaloblastic Anemias Hematology Lect Megaloblastic Anemia, Pernicious Anemia Hematology Lab Abnormal Differentials: Anemias Reading Assignment: Chapter 11
Slide Set #1 Abnormal RBC and Anemia [abnrbcsl.doc]

February 12

Week 5: RBC Disorders-Hemolytic Anemias/Intrinsic/Extrinsic Hematology Lect Hemolytic Anemia: Intrinsic Hemolytic Disease Hematology Lect Hemolytic Anemia: Extrinsic Hemolytic Disease Hematology Lab Abnormal Differentials: Anemias Reading Assignment: Chapter 16, 17, 18, 19, 20
The Hemolytic Disorders [hemodiso.doc] Paroxysmal Cold Hemoglobinuria Check Sample Case [PCH check sample case.pdf] Hematology Case 3-1 [case1.pdf]
Graves, et.al., Case Studies in Clinical Laboratory Science, Prentiss Hall, 2002, ISBN 0-13-088711-0

Hematology Case 3-2 [case2.pdf]

Graves, et.al., Case Studies in Clinical Laboratory Science, Prentiss Hall, 2002, ISBN 0-13-088711-0

Answers to Cases 3-1 and 3-2 [case 1-2 answers.pdf]

Graves, et.al., Case Studies in Clinical Laboratory Science, Prentiss Hall, 2002, ISBN 0-13-088711-0

February 19 February 26

NO CLASS CLEC CONFERENCE Week 6: RBC Disorders- Hemolytic Anemia/Thalassemia/Hemoglobinopathy Hematology Lect Hemoglobinopathy: Defective Hemoglobin Synthesis Hematology Lect Thalassemia: Diminished Hemoglobin Chain Production Hematology Lab RBC Indices/ Sickle-Dex Test for Hgb S/Slide Review Reading Assignment: Chapter 12, 13
On Line Examination #1 (Obtain password from instructor to take this test.) --------------------Hemoglobinopathy and Thalassemia (Specific Objectives) [hgbtobj.doc] Hemoglobinopathy and Thalassemia [hgbthal.doc] Hemoglobinopathy and Thalassemia (Olesinski, 2001) [thalhgbopathy.ppt] Qualitative Solubility Test for Identification of Sickling Hemoglobin [sickledx.doc] -

March 5

Week 7: WBC Disorders- Leukemoid Reactions Hematology Lect Leukocyte Disorders, Qualitative/Quantitative Hematology Lect Leukemoid Reactions/Myeloproliferative Disease Hematology Lab Automated Cell Counting in the Hematology Laboratory Reading Assignment: Chapter 21, 22
The Red Cell and Anemia 1995-96, Edward O. Uthman [redcell&anemia.pdf] Morphological Alterations of Leukocytes [moralneu.doc]

Hematology Case 1 (Coulter Instruments) [coulter case1.pdf] Hematology Case 3 (Coulter Instruments) [coulter case3.pdf] Hematology Case 10 (Coulter Instruments) [coulter case10.pdf]

March 12

Week 8: WBC Disorders-Polycythemia/Chronic Leukemia Hematology Lect Polycythemia/Chronic Leukemia Hematology Lect Polycythemia/Chronic Leukemia Hematology Lab Abnormal RBC Morphology The ABX multiparameter analyzer Reading Assignment: Chapter 26, 27, 41
Automated Cell Counting in the Hematology Laboratory [celcount.doc] Flow Cytometry [flow cytometry.doc] Scatterplot Interpretation [scatterplot interpretation.pdf] Flow Cytometry Panels CD Antigens Useful for Hematopathology Reference Ranges for Peripheral Blood Lymphocyte Populations Chronic Myeloproliferative Disorders (other than leukemia) [myloprol.doc] Primary and Secondary Polycythemia Bone Marrow Aspiration and Biopsy Bone Marrow Aspiration (Wiggers) [bmaspira.doc] Classification of the Neoplastic Disorders [neoplast.doc] Chronic Myelocytic Leukemia [hemodiso.doc] Hematology Case 16 (Coulter Instruments) [coulter case16.pdf] Chronic Myelocytic Leukemia Additional Information Chronic Lymphocytic Leukemia [chrolyml.doc] Hematology Case 2 (Coulter Instruments) [coulter case2.pdf] Chronic Lymphocytic Leukemia Additional Information

March 19 March 26

NO CLASS - Spring Break Week 9: WBC Disorders-Acute Leukemia / Lecture Exam #2 Hematology Lect Acute Leukemia Hematology Lect Acute leukemia Hematology Lab Lecture/Laboratory Examination #2 Abnormal Differentials: WBC Reading Assignment: Chapter 29, 30
Leukemia Overview [leukemia.doc] Lymphoma Overview [lymphoma.doc] Classification of Leukemias [leukbldp.doc] On Line Examination #2 (Obtain password from instructor to take this test.) ----------------------

April 2

Week 10: Acute Leukemia/Cytochemistry Hematology Lect Acute Leukemia Hematology Lect Special Stains/Cytochemistry/Markers Hematology Lab Leukemia Differentials Reading Assignment: Chapter 26
Cytochemical Staining Techniques [splstain.doc] Cytochemical Staining Techniques (Univ. of Washington) Acute Myelocytic Leukemia [acutemyl.doc] Diagnosis of Acute Myeloid Leukemias Hematology Case 18 (Coulter Instruments) [coulter case18.pdf] Acute Myelocytic Leukemia Additional Information Acute Lymphocytic Leukemia [acutelyl.doc] Hematology Case 7 (Coulter Instruments) [coulter case7.pdf] Acute Lymphocytic Leukemia Additional Information Hematology Case 19 (Coulter Instruments) [coulter case19.pdf] Hematology Case 20 (Coulter Instruments) [coulter case20.pdf]

April 9 April 16

NO CLASS - Easter Holiday Week 11: Special Stains Hematology Lect Special Stains/Cytochemistry/Markers Hematology Lect Special Stains/Cytochemistry/Markers Hematology Lab Leukemia Differentials Reading Assignment: Chapter 26
Hairy Cell Leukemia Myeloma and Plasma Cell Leukemia Mycosis Fungoides Myelodisplastic Syndromes Miscellaneous Blood Disorders [haircell.doc] Hematology Case 5 (Coulter Instruments) [coulter case5.pdf] Hematology Case 6 (Coulter Instruments) [coulter case6.pdf] Blood Protein Disorders, SLE [protdis.doc] Waldenstrom's Hypergammaglobulinemia Additional Information Systemic Lupus Erythematosus Additional Information Abnormal Hemoglobin Pigments [abnorhgb.doc] Methemoglobinemia Additional Information Porphyrias [porphyr.doc

April 23

Week 12: Basic Coagulation Hematology Lect The Coagulation Cascade Hematology Lect The Coagulation Cascade Hematology Lab Coagulation Cascade Problemsolving Reading Assignment: Chapter 34, 35, 36-39
Blood Coagulation [coagulat.doc] The Prothrombin Time [pt&ptt.doc] Disorders of Coagulation [coagdiso.doc] PT and PTT Procedures [coagptptt.pdf] Platelet Disorders Additional Information Hemostatic Disorders, Nonplatelet, Additional Information Disseminated Intravascular Coagulation Additional Information

April 30

Week 13: Basic Coagulation Hematology Lab 1:30-5:00 pm

Coagulation Laboratory/ Mrs. Wilkins

On Line Examination #3 (Obtain password from instructor to take this test.) ----------------------

May 4-8

Week 14:

Final Comprehensive Examination (Obtain password from instructor to take this test.) ----------------------

81095051.doc Thursday, January 12, 2012