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WILLIE GIRALD-ROSA, MS, PhD Urb Costa Brava Calle Finche M295, Isabela PR 00662 e-mail: [1]wg117ca38@westpost.

net Phone: 787-872-7769 Cell phone 787-546-0714 Objectives: To improve my skills and acquire more knowledge throughout experience. Education: 2010 PhD in Molecular Biology and Gene Therapy Universidad Autnoma de Madrid (UAM) (Autonomous University of Madrid) Department of Molecular Biology Center of Molecular Biology Severo Ochoa (CBMSO) Doctoral Thesis The linamarase/linamarin/glucose oxidase system as a Potential Treatment for Glioblastoma in the Nude Rat Brain. Other Research Projects Characterization of cancer stem cells of brain tumors isolated from human patients. The effect of the nucleostemin gene in the development of gliomas in the brain of nude rats. 2002-2005 Graduate Research Assistant Vanderbilt University, School of Medicine-Division of Dermatology Research Project Mitochondrial Point Mutations in Non-melanoma Skin Cancer: Possible Selection in Tumorigenesis. June 1998 University of Puerto Rico, Mayaguez Campus Master Science; Biology; Specialized in Molecular Genetics and Molecular Virology. Graduated Magna Cum Laude. Master Science Thesis Characterization of Parvovirus LuIII Proteins and The Interaction of Viral and Cellular Proteins with LuIII DNA Sequences.

Relevant Graduate Courses: Cellular Biochemistry and Physiology Phytovirology Genetic Transformation Molecular Virology Molecular Genetics May 1994 University of Puerto Rico, Arecibo Campus Bachelor Degree: Biology specialized in Microbiology Graduated Cum Laude Relevant Undergraduate Courses: General Microbiology General Biochemistry Medical Mycology Industrial Mycology Immunology Genetics of Bacteria Molecular Biology Bacteriology Water Microbiology General Biochemistry Experience: December 2007- July 2010 PhD Student in Molecular Biology and Gene Therapy Universidad Autnoma de Madrid (UAM) Department of Molecular Biology Center of Molecular Biology Severo Ochoa (CBMSO) January 2006-December 2007 Instructor of Molecular Cell Biology, Immunology and Virology Department of Science and Technology Interamerican University of Puerto Rico, Aguadilla Campus April 2002-December 2005 Graduate Research Assistant of the Bridges Program Vanderbilt University School of Medicine Division of Dermatology August 2001-December 2001 Instructor of Genetics and Virology

Interamerican University of Puerto Rico San Germn Campus Department of Science and Technology August 1998-May 1999 Instructor of Biochemistry and Genetics Interamerican University of Puerto Rico, Arecibo Campus Department of Science and Technology August 1999-May 2001 University of Puerto Rico, Bayamn Campus Department of Biology Instructor of Genetics, Modern Biology, Molecular Biology and Research Courses in Molecular Genetics. Participation on University Committees: President of Biosafety Committee, UPR-Bayamon (1999-2001) Professional Improvement Committee, UPR-Bayamon (1999-2001) Committee for the Development of the Proposal for the Bechellor in Biotechnology, UPR-Bayamn, 2000-2001 1994-1998 University of Puerto Rico, Mayaguez campus Teacher assistant: laboratories of General Genetics, Molecular Genetics and Virology. 1996-1998 University of Puerto Rico, Mayaguez campus Department of Biology Research assistant: Molecular Virology and Genetics Labs Skills: Knowledge in Molecular Genetics, Biochemistry and Molecular Biology technical equipments like: PCR machines, Electrophoresis, Electroporation, Spectroscopy and Mass Spectrophotometer, Flow Cytometry, TGCE Machine, Stereotaxic instruments, Xenotransplantation of brain tumors. Experiments: Studies of transcription factors, cellular and nuclear extracts, DNA-Binding Shift Assays, tissue cultures of embryonic stem cells, viral infections, Cloning, Western Blot, Viability Cell Assay, Temperature Gradient Capillary Electrophoresis, eukaryote and prokaryote cell transformations, development of cybrids, Virotherapy and Gene Therapy, Stereotaxic Brain Surgery in Rats, Characterization

of Glioma Cancer Stem Cells. Domain in the following languages: Spanish and English Activities: Active member in the following organizations: American Society of Human genetics (ASHG) American Society for Investigative Dermatology American Association for Cancer Research American Society of Clinical Oncology Honors: First Price in Organic Chemistry Olympics, UPR Graduated Cum Laude from the Univeristy of Puerto Rico, Arecibo Campus (BS) Graduated Magna Cum Laude from the University of Puerto Rico, Mayaguez Campus (MS) Graduated Magna Cum Laude from the

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