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PUNJABI UNIVERSITY APPROVED SYLLABUS 2011, 2012 & 2013

Syllabus for Certificate Course in Plant Biotechnology (Add on Course) Ist Year: Theory Paper Max. Marks 60 Time Allowed 3 hrs. Pass Marks 35% of the Subject, Theory and Practical Separately INSTRUCTIONS TO THE PAPER SETTER Paper should be divided into A,B,C,D and E. Section A,B,C and D shall have two questions, each student must atttempt at least one question from each part. Fifth section E shall have 10 short answer type questions of 2 marks each covering the entire syllabus students will attempt all the 10 questions of Section E. Questions of section A,B,C and D will carry 10 marks each. The answers of short questions should be given in 2-3 lines. INSTRUCTIONS TO THE CANDIDATES: Candidates are required to attempt one question each from the section A,B,C and D of the question paper and the entire section E. SECTION A 1. 2. 3. 4. Introduction to Plant Biotechnology and its importance. Brief introduction to plant cell structure and functions of organelles. SECTION B Introduction to plant tissue culture, Lab equipments and their Various sterilization and preparation techniques, used in plant tissue culture. SECTION C 5. 6. Media composition and preparation. Cellular totipotency, Initiation, propagation and maintenance of : a) b) Callus Culture Suspension Culture working. Lectures to be delivered : 50 (each of 45 min. duration periods/week: 3)

2 SECTION D 7. 8. Introduction to transgenic plants. Introduction to secondary metabolites and industrial products. PRACTICAL PAPER Max. Marks : 40 Pass Marks : 35% of subject Theory and Practical separately 1. 2. 3. 4. 5. 6. 7. 8. 9. 1. Lectures to be delivered 100 (each of 45 min. duration: Periods /week : 6)

Permanent slide preparation of plant cell. Introduction to lab equipment Autoclave, LAF, Microscope. Sterilization techniques Dry and Hot. Preparation of Plant Tissue Culture Media. Explants Preparation. Callus Induction. Artificial pollination and fertilization. Practical Record Viva Voce RECOMMENDED READINGS Principles of Plant Biotechnology- An Introduction of Genetic Engineering in Plants by S.H. Mantell, J.W. Mathews and R.A. Mckee, Blackwell Scientific Publications.

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Plant Cell, Tissue, Organ Culture: Fundamental Methods by O.L. Gamborg and G.C. Phillips, Narosa Publication, House, New Delhi. Plant Biotechnology and Tissue Culture: Principles and Perspective, Edited by. A. Kumar and V.A. Kumar, Introduction Book Distributing Company, Lucknow.

4. 5. 6. 7. 8.

Cell Biology (India Ed.)- C.P. Powaar (2003). Plant Cell Culture A Practical Approach Eds. R.A. Dixon and Gonzales, IRL Press. Biotechnology: Principles and Applications, I.J. Higgins, D.J. Best and J. Jones, Blackwell Scientific publications. An Introduction to Plant Tissue Culture M.K. Razdan, Oxford and IBII Publishing Co. Pvt. Ltd., New Delhi. Fundamentals of Plant Biotechnology by Amla Batra Capital Publishing Company.

3 9. Introduction to Plant Tissue Culture, Bhojwani and Rozdan.

10. A textbook of Biotechnology by R.C. Dubey. 11. Plant Biotechnology by K.G. Ramawat. ORDINANCES The Certificate Course/Diploma/Advanced Diploma in Plant Biotechnology; career Oriented Programme of UGC 2003-2004 introduced at First Degree Level in B.A./B.Sc. 1. The admission/examination shall be opened to any candidate who has passed 10+2 examination with at least 45% marks in aggregate in science. 2. The candidate after passing examination will be awarded a separate "Certificate Course/Diploma/Advanced Diploma in Plant Biotechnology" in the addition to his/her regular de+gre+e /D.M.C. of B.A./B.Sc. 3. The supplementary examination shall be held in September or as fixed by the Academic Council. This examination shall be opened to candidates who have been declared reappear in Certificate Course/Diploma/Advanced Diploma. 4. The candidate who doesn't pass in the supplementary examination will be given another chance to appear in above said course along with forthcoming annual examination. 5. A candidate who passed the course in the supplementary examination or in the third chance in annual examination can appear alongside in next subsequent examination of above said course. 6. 7. 8. 9. The candidate who is unable to pass the course in three given chances, will not be allowed to continue the above course. Every candidate will be required to attend minimum of 75% lectures/periods delivered to that class. The candidate must obtain 35% of the total marks in theory and practical separately to pass the course. The candidate must have obtained in House Examination at least 25% marks in subject.

4 10. Candidates will be offered English as the medium of

Instruction/Examinations. *****************