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GLYCOFILM: AN ANTI-AGING SHIELD WITH A SECOND SKIN EFFECT

BIOTECHNOLOGICAL POLYSACCHARIDE

BY SOLABIA

www.solabia.com

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Aging is not always inevitable... Although we cant stop intrinsic aging, which is genetically programmed, extrinsic aging can be delayed and we can, maybe even should, fight against it. Extrinsic aging is also known as photo-induced aging, meaning it is induced by the suns rays, in particular ultra-violet. However, this name often makes us forget that it can be induced by other environmental factors, in particular pollution. While the harmful effects of pollution on our health were discovered several years ago, the role they play in the premature appearance of wrinkles and pigment spots has only lately come to light.

ANTI-AGING: POLLUTION AND SUN, THE SAME FIGHT! POLLUTION

A trial recently carried out in Germany1, on 400 women aged between 70 and 80, highlighted a link between long-term exposure to air pollution and extrinsic skin aging. According to this trial, the greater the level of air pollution (quantity of soot and high number of carbon particles), the more likely it is that the skin will experience pigmentation problems and have a higher number of wrinkles (particularly in the naso-labial fold). In particular, an increase of 20% in pigment spots on the forehead and cheeks was noted on the most exposed skin. These results can be explained by several factors. Firstly, the ambient particles become embedded in the skin, causing oxidative stress, which as we already know is responsible for a cascade of inflammatory reactions, in particular damage to the components of the dermis (collagen, elastin etc.). Secondly, the ambient particles act as carriers of carbon and metal components such as polyaromatic hydrocarbons, which may be converted into quinone, which itself causes oxidative stress. Finally, the newest hypothesis is that the polyaromatic hydrocarbons associated with ambient particles have an affinity for certain keratinocyte and melanocyte receptors (AhR receptors = Aryl hydrocarbon Receptor). Over-stimulation of the receptors increases the anarchic production of melanin, which leads to hyperpigmentation and the appearance of pigment spots. Inhibiting or blocking these receptors could be a way of slowing down the extrinsic aging caused by pollution. However, beyond this biological activity, it is also possible to act physically against the embedding of carbon particles in the skin, using a protective cosmetic shield.

Airbone particle exposure and extrinsic skin aging. A.Vierkotter, T.Schikowski and al. Journal of Investigative Dermatology (2010) 130, 2719-2726

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SUN
UVA and UVB rays are responsible for many harmful effects on the skin: damage to cell membranes, irreversible changes in the genetic make-up of the cells and the structure of proteins (which become inactive), weakening of the skins immune surveillance mechanism In the epidermis, after exposure to the sun, the keratinocytes stop dividing and start to mature, leading to a thickening of the epidermis, a natural protective phenomenon. However prolonged and repeated exposure throughout a persons life can disturb maturation and scaling of the keratinocytes and disorder the melanocytes, leading to premature formation of pigment spots. When the skin is sunburnt, the inflammatory process takes place very quickly, and subsequently tissue lesions appear in the epidermis, leading to the appearance of Sun Burn Cells (keratinocytes with an abnormal morphology likely to lead to cell death and elimination by accelerated scaling). Although one incidence of sun burn may not have much consequence, an accumulation of incidents will in time damage the natural defence processes and accelerate extrinsic aging: premature cell death, accumulation of Sun Burn Cells, and chronic inflammation, which will in turn damage the components of the dermis... Here again, beyond biological activity (inhibition of inflammatory phenomena, protection of collagen etc.), it is also possible to physically protect the skin against UV by using filters, anti-free radical agents and a protective cosmetic shield.

GLYCOFILM: A SECOND SKIN PROTECTIVE MATRIX

To meet this need, the SOLABIA Groups research scientists have used a biotechnological process to produce a natural desacetylated polysaccharide with a high molecular weight, and a protective film-forming, non-occlusive effect. In fact, certain desacetylated polysaccharides can form highly organised D-glucose and resistant matrix networks regardless of Glucuronic acid intrinsic gelling properties. Presenting an affinity for bivalent salts, ethanol and glycols, Glycofilm can form, combined with these ingredients, a more stable and better organized matrix network. This specific shield has the properties of melting on the skin, to let the feeling of a second skin. Capable of reducing the embedding of carbon / heavy metal particles in the skin and protecting the cells against UV rays, Glycofilm is the perfect anti-aging shield.
L-fucose

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PROTECTION AGAINST ENVIRONMENTAL AGGRESSIONS

EVALUATION OF ANTI-POLLUTION PROPERTIES

On carbon particles
In vivo study on 10 volunteers / Application of a cream containing Glycofilm (1%, 3% or 5%) vs Placebo and then a carbon particles solution creating the urban pollution conditions / Visualization by videomicroscopy and quantification by image analysis of the carbon particles on the skin before and after rinsing / Deduction of the protection percentage due to Glycofilm against pollution vs Placebo.

Placebo, before rinsing

50
Anti-pollution protection (%) vs Placebo

Placebo, after rinsing

45 40 35 30 25 20 15 10 5 0 Glycofilm 1% Glycofilm 3% Glycofilm 5%

1% GLYCOFILM , before rinsing

46 38 30

1% GLYCOFILM, after rinsing

Protective effect of GLYCOFILM against pollution (vs Placebo)

IN

THE STUDY CONDITIONS, THE CRE AM CONT AINING WELL PROTECTIVE EFFECT AG AINS T POLLUTION. VS PLACE BO, THE SKIN IS MORE PROTECTED BY CONT AINING

GLYCOFILM

PRESENTS A

GLYCOFILM AND MORE PROTECTED G L Y C O F I L M . AS A PROTECTIVE SHIELD GLYCOFILM OFFERS A DOSE-DEPENDENT EFFICACY A G A I N S T P O L L U T I O N , A V O I D E D T H E E M B E D D E D O F C A R B O N P AR T I C L E S I N T H E S K I N AND SO ON ITS CONSEQUENCE ON EXTRINSIC AGING.

1 % G L Y C O F I L M ,

BY

30 % W I T H T H E C R E A M 38 % W I T H T H E C R E A M C O N T A I N I N G 3 % B Y 46 % W I T H T H E C R E A M C O N T A I N I N G 5 %

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On heavy metals
In vitro study on reconstructed epidermis / Topical application of Glycofilm at 5% vs Control (NaCl at 0,9%) / Incubation / Washing and treatment by a solution without or with CdCl2 (1mM, 2 mM, 5mM) / Evaluation of the epidermal cells viability by the MTT test. Concentration of cadmium (mM) Mean cellular viability (%) 0 100 CONTROL 1 2 99 77 5 48 5% GLYCOFILM 0 100 1 98 2 89 5 75

Effect of Control on cell viability following cadmium aggression

Concentration of Cadmium (mM) Mean cellular viability (%) UNDER

Protective effect of Glycofilm on cell viability following cadmium aggression THESE TEST CONDITIONS, GLYCOFILM EXHIBITS A PROTECTIVE EFFECT AG AINST TOXIC C ADMIUM CONCENTR ATIONS (IMPROVEMENT OF ME AN CELLUL AR V I A B I L I T Y V S C O N T R O L ) F O L L O W I N G T O P I C A L AP P L I C A T I O N . T H I S P R O T E C T I V E EFFECT CAN BE ATTRIBUTED TO THE REDUCED PENETRATION OF C ADMIUM IN THE EPIDERMIS SINCE THE C ADMIUM IS TRAPPED BY THE FILM FORMED BY THE POLYS ACCHARIDE ON THE SURFACE OF THE EPIDERMIS.

In brief
GLYCOFILM ACTS AS AN ANTI-AGING SHIELD BY PROTECTING THE SKIN FROM THE CONSEQUENCES POLLUTION, SUCH AS: - EMBEDDING OF CARBON PARTICLES IN THE SKIN, RESPONSIBLE FOR INCREASED FORMATION
WRINKLES AND PIGMENT SPOTS - INTOXICATION OF CELLS WITH HEAVY METALS, RESPONSIBLE FOR KILLING OFF CELLS OF OF

EVALUATION OF PROTECTIVE PROPERTIES AGAINST UV RAYS

In vitro study on reconstructed epidermis / Topical application of Glycofilm at 5% vs Control / UV irradiation / Incubation / UV protector effect defined by the measure and observation of 3 factors : - dosage of deshydrogenase lactate (LDH, enzyme testifying of membrane alteration) - dosage of IL1 released (inflammation molecule) in culture medium (Elisa kit) / - coloring, visualization of histological sectioning and enumeration of Sun Burn Cells.

On damage to cell membranes

GLYCOFILM
PRESENTS A SIGNIFICANT PROTECTIVE EFFECT AG AINST CELLULAR MEMBR ANES A L T E R A T I O N I N D U C E D B Y U V , V S C O N T R O L : + 6 8 %.

Concentrations Control 5% GLYCOFILM Control + UV 5% GLYCOFILM + UV

LDH (O.D.) 0.15 0.18 1.33 0.43

Protective effect of Glycofilm on LDH synthesis

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On inflammatory reactions
GLYCOFILM ALLOWS A IL1- R E L E A S E I N D U C E D - 53 %, A L L O W I N G T O CH AIN RE ACTION.
SIGNIFICANT INHIBITION OF BY UV, VS MODUL ATE

Concentrations Control 5% GLYCOFILM Control + UV 5% GLYCOFILM + UV

IL1- (pg/mL) 15.3 5.5 15.6 0.35 188 35 88 5

CONTROL:
I N F L AM M A T I O N

On the appearance of Sun Burn Cells

Concentrations Control GLYCOFILM 5% Control + UV GLYCOFILM 5% + UV Sun-burn cells counting 1 5 30 19

Protective effect of Glycofilm on IL1 release

Example of Sun Burn Cells

Protective effect of Glycofilm on Sun Burn Cells number

GLYCOFILM ALLOWS TO REDUCE B Y U V , V S C O N T R O L : -37 %.

THE FORM ATION OF

SUN BURN CELLS

INDUCED

Glycofilm can boost the SPF

In vivo study on 10 volunteers / Application on back of creams containing Glycofilm (3 or 5%) associated or not to matrix co-activators (NaCl or PEG8) vs Placebo / UV irradiation / Evaluation of SPF 18
16

Effect of Glycofilm on the SPF increase

Sun Protective Factor

14 12 10 8 6 4 2 0 No association Association with NaCl

15,3 15,8 12,4 10,8 12,9 13,4 11,7 11,2 13,3 Control 3% Glycofilm 5% Glycofilm

Association with PEG-8

G L Y C O F I L M A L O N E A L L O W S T O E N H A N C E T H E SPF B Y A R O U N D 1 P O I N T THE COMBIN ATION OF GLYCOFILM WITH INGREDIENTS REINFORCING ITS E F F E C T A L L O W S T O E N H A N C E T H E SPF B E T W E E N 2 A N D 3 P O I N T S

M ATRIX

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In brief
GLYCOFILM ACTS AS AN ANTI-AGING SHIELD BY PROTECTING THE SKIN FROM THE CONSEQUENCES OF THE SUN, SUCH AS: - DAMAGE TO CELL MEMBRANES, RESPONSIBLE FOR ACCELERATING PREMATURE CELL DEATH - CHRONIC INFLAMMATORY REACTIONS, RESPONSIBLE FOR EXHAUSTING THE CELL DEFENCE
MECHANISMS AND DAMAGING THE COMPONENTS OF THE DERMIS - THE FORMATION OF SUN BURN CELLS WHICH CAUSE PREMATURE CELL DEATH AND MAY ACCUMULATE IF THEIR ELIMINATION PROCESS IS BLOCKED

CONCLUSION
Preventive and/or protective action is essential to combat aging, as in many cases it can minimise the consequences of external stresses on the skin (UV and pollution) and therefore slow down the extrinsic aging process, reducing the need for action by the skin's defence mechanisms, which if used too often will weaken over the years. Due to its capacity to form a shield on the skins surface, Glycofilm meets this need for protection. Due to its second skin effect, it gives invisible, yet effective protection!

GLYCOFILM AN ANTI-AGING SHIELD WITH A SECOND SKIN EFFECT!

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