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The colloidal portion (sub-microscopic particle size, large surface area) of soils consists of highly decomposed particles of clay and organic matter, and account for a soils capacity to hold nutrient elements. These minute clay and organic colloids have a net negative charge, and therefore attract and hold iron, manganese, zinc, and copper. The positively charged metals are called cations, and the capacity of a particular soil to hold such cations is called the Cation Exchange Capacity (CEC). Hence, the capacity of a soil to hold metal cations varies directly with the CEC of individual soils. The CEC varies considerably from soil to soil depending on the type and percent of colloidal clay and organic fractions present in a given soil. For example, a soil in which sand is the predominant mineral component, would have a low CEC as opposed to a clay soil with a relatively high CEC. Soils high in colloidal organic matter have an even higher CEC than clay soils. Since these metal cations of a soil can be displaced through chemical extraction, the CEC of a particular soil can be determined with the proper soil test. As a result, the native fertility level of the soil can be assayed and evaluated in light of the extractable nutrient content as it related to the CEC of that soil. For example, soils classified as loams, sandy clays, silt loams, etc. have a much higher CEC than sands, sandy loams, or loamy sands, and are less subject to nutrient leaching. Consequently, the extractable nutrient fraction from these soil classes are generally much higher, which results in a higher fertility status and residual benefit. In cases where both fertility status and residual nutrient supplies are high, the need for supplemental fertilizer treatment could be reduced or in unique cases eliminated entirely.
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Permeability The permeability of a rock or sediment refers to its ability to transmit groundwater freely. The rate at which a material transmits water depends not only on its total porosity, but also on the size of the passageways between its openings. To be considered permeable, the open spaces in a rock must be connected. The size and sorting of the particles composing the rock or sediment will affect its permeability. Generally, materials of larger particle size that are consistently sorted will be more permeable. A rock can have high porosity, but low permeability if the open spaces are not well connected. For example, while clay may display a higher porosity than sand, the clay particles are much finer and the spacing between them is very small. As a result, while the clay may hold more water, the water is transmitted more readily through the sand than through the more porous clay. This is principally due to the fact that the molecular attraction on the water is much stronger in the tiny openings between clay particles. The passageways between particles in the sand are relatively large, and the molecular attraction on the water is relatively low, allowing the water to move more freely in this material Friction slows down the flow of water through rock. Well-sorted, large grained rocks and sediments have less surface area to cause friction during the waters passage than do finer grained materials. Rocks composed of coarse grained particles, like sandstone, are often the most permeable. However, even smaller grained rocks, like limestone, can be permeable due to the presence of interconnected cracks and enlarged fractures due to solution. Regardless of how large the spacing is between particles in a rock or sediment, they must be interconnected for water to pass through. Materials through which water cannot flow are called impermeable. Clays, shale, and most metamorphic and crystalline igneous rocks are often impermeable or are poor aquifers. The most effective aquifers are typically unconsolidated sand and gravel, sandstone, and some limestone. Permeability, then, plays a critical role in determining the rate at which groundwater flows through an aquifer. Where permeability is high, the groundwater passes quickly through the ground; when permeability declines, the flow is reduced. Nutrients The major essential nutrient elements supplied through the soil are Nitrogen (N), phosphorous (P), and potassium (K). Nutrients absorbed from the soil by plants are supplied by decomposition of rock, decomposition of organic matter, deposition by flood waters, application of commercial fertilizers, and the use of animal or plant manures. Nitrogen is the most abundant element in the atmosphere (about 80%), but the gaseous form cannot be absorbed by plants. However, a relatively large group of plants , the legumes, have root nodules that contain Rhizobium bacteria which convert gaseous nitrogen into a form usable by plants (nitrogen fixation). Nitrogen promotes succulence in forage crops and leafy vegetables. It stimulates above-ground growth, hastens crop maturity, and is very influential in fruit sizing. Phosphorous is necessary for the hardy growth of the plant and activity of the cells. It encourages root development, and by hastening the maturity of the plant, it increases the ratio of grain to straw, as well as the total yield. It plays an important part in increasing the palatability of plants and stimulates the formation of fats, convertible starches, and healthy seed. By stimulating rapid cell development in the plant, phosphorous naturally increases the resistance to disease. Potassium is not a component of the structural makeup of plants, but it plays a vital role in the physiological and biochemical functions of plants. The exact function of potassium in plants is not clearly understood, but many beneficial factors implicating the involvement and necessity of potassium in plant nutrition have been demonstrated. Some of these factors are: it enhances disease resistance by strengthening stalks and stems, activates various enzyme systems within plants, contrinbutes to a thicker cuticle which guards against disease and water loss, controls the turgor pressure within plants to prevent wilting, enhances fruit size, flavor, texture, and development, and is
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It is important to note that plants need more than these three nutrients to grow and reproduce successfully. Carbon, hydrogen, and oxygen from water and atmospheric carbon dioxide are used in abundance and other macronutrients like calcium, magnesium, and sulfur are crucial. Calcium is a component of cell walls and is known to stimulate root and leaf development as well activate several enzyme reactions. It also maintains optimum pH levels in the plant by neutralizing many of the organic acids generated by plant respiration. Sulfur and magnesium are both involved in the formation of chlorophyll. Photosynthesis cannot occur without these nutrients. Yellow spots on the leaves of houseplants are commonly the result of the lack of magnesium. Furthermore, there are a number of nutrients that plants require in much smaller quantities. These include manganese, iron, boron, copper, zinc, molybdenum, and chlorine. A healthy, balanced soil should contain appropriate levels of all of these nutrients. Soil pH A pH measurement of soil is a measure of acidity that is present in the soil solution, and does not address the total acidity present in the soil. Total acidity includes the acidity of the soil solution as well as the hydrogen which is held on the soil colloid. These two forms of acidity have been broken down into two categories, active acidity (measured with a pH meter) and reserve of exchangeable acidity (acidity held on the soil colloids). The latter form of acidity must be displaced by chemical means (generally a neutral buffered salt) before it can be effectively measured. Since these two forms of acidity are in equilibrium with each other in the soil, a pH depression will be observed as the reserve acidity in the soil increases. The reserve acidity is used in determining the amount of limestone required for optimum plant growth. Since the makeup of soils is alumino-silicates, aluminum is a major contributor to soil acidity. Therefore, the rate of lime required to achieve optimum plant growth can be determined by measuring exchangeable aluminum in the soil. Exchangeable aluminum, like exchangeable acidity, decreases with the increases in soil pH. The pH at which aluminum is rendered ineffective is generating acidity is around pH 5.5. The pH measurement is a simple means by which the production potential of a soil can be evaluated. For example, soils in which the pH is extremely low, have correspondingly low calcium and magnesium levels with high levels of exchangeable acidity. All of the above factors have adverse effects on plant growth. In addition, at low pH levels, metal cations such as aluminum and manganese are much more soluble and can reach levels which are toxic to plants. These toxic components can be eliminated entirely by application of limestone to raise the pH. In addition to the reduction in the solubility of toxic metals, increased pH levels also enhance microbial activity within the soil. Hence, the key to good crop production is to maintain the pH within the range where plants and microbiological activity within the soil can function at their optimum level. For most soils this requires a pH between pH 6.0 and 6.5. Soil pH levels higher than 6.5 can create problems with certain micronutrients (manganese in particular).
MATERIALS
100 ml graduated cylinders 50 ml graduated cylinders electronic balance filter paper funnels test tubes test tube racks water bottles parafilm methyl orange
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PROCEDURE
Group One Soil Texture by Fractionation Soil texture can be determined by fractionation. Sand is larger so it will settle out faster in a suspension, silt is the next in size so it settles out next. Clay is made up of the smallest particles so they will settle on the top. Try this experiment: 1. 2. 3. 4. 5. Fill a 100 ml graduated cylinder with 25 ml of your soil sample. Add water until there is about 75 ml in the cylinder. Cover the cylinder with film and invert several times until the soil is thoroughly suspended in the water. Place the cylinder on the lab station and leave it to settle overnight When the soil has settled out, there should be 3 distinct layers. Measure the volume of each layer and the total volume of the sample. Calculate the percentage of each component. Amount of each component x 100 = % component Total volume of soil Identify the type of soil in your sample by using the soil texture triangle. Record your result in Table 1.
6. 7.
Group Two Cation Exchange Capacity (CEC) Soil can absorb and release cations for plant nutrients because of the electronegativity found on clay and humus particles. Certain chemical dyes possess a positive or negative charge and can simulate the behavior or the typical anions (NO3- and Cl-) and cations (K+, H+, Ca++, Mg++, and Al++) found in soil. In this experiment, we will be using methyl orange which has a negative charge and methyl red which has a positive charge. 1. 2. 3. 4. Put filter paper in two funnels and add 20 ml of your soil sample. Place the funnels in the large test tubes. Pour 20 ml of methyl orange in one of the funnels. Pour 20 ml of methyl red in the other funnel. Record your results in Table 1 as degree of color change (lighter, darker, or no change)
Group Three Density and Porosity Determining soil density is one way of quantifying porosity. Dense soils have low porosity. Soils can become dense due to the compaction of heavy equipment or traffic. 1. 2. 3. 4. 5. 6. Weigh out 15 grams of your sample. Pour it into a dry 50 ml graduated cylinder. Tap gently on the table to settle the particles. Determine the volume of soil in the cylinder. Add water until the soil is completely saturated. Make sure to keep track of how much water is added. Determine the final volume by subtracting the volume of water added from the volume of soil in the graduated cylinder Use the table below to calculate your results and record the final density values in Table 1.
Sample 1
Weight (g) 15
Volume (ml)
Density (g/ml)
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15 15 15 15
Group Four Water Holding Capacity of Soil - Permeability 1. 2. 3. Fold a piece of filter paper and place it in the funnel. Put 20 ml of your sample into the funnel. Hold funnel of soil over a beaker and pour 50 ml of water into the funnel. Time how long it takes for the water to begin coming out of the bottom, and the times it takes the water to stop coming out of the bottom. The time interval indicates the permeability of the soil. Record your results in Table 1.
4.
Group Five Nitrogen and Phosphorous Test for Nitrogen: 1. 2. 3. 4. 5. 6. 7. Fill a test tube (0755) to line 7 with Universal Extraction Solution (5173). Use soil measure (0819) to add one level measure of soil. Cap and shake for one minute. Fold a piece of filter paper (0465) in half. Fold in half again. Gently push the corners together to form a cone. Place cone in funnel (0459). Place funnel in clean filtrate tube (0749) and filter suspension through the filter paper. The clear solution is the extract. Use the 1 ml pipet (0354) to transfer 1 ml of soil extract to one of the depressions on the spot plate (0159). Use the 0.5 g spoon (0698) to add one measure of Nitrate Reagent #2 (5147). Stir thoroughly with plastic rod. Wait five minutes. Match sample color to a color standard on Nitrate-Nitrogen color chart (1315). Record as lbs/acre nitrate-nitrogen.
Test for Phosphorous 1. Fill a test tube (0755) to line 7 with Universal Extraction Solution (5173). 2. Use soil measure (0819) to add one level measure of soil. Cap and shake for one minute. 3. Fold a piece of filter paper (0465) in half. Fold in half again. Gently push the corners together to form a cone. Place cone in funnel (0459). 4. Place funnel in clean filtrate tube (0749) and filter suspension through the filter paper. The clear solution is the extract used in the test. 5. Fill the Phosphorous B Tube (0244) to the mark with the soil extract. 6. Add 6 drops of Phosphorous Reagent #2 (5156) and swirl to mix. 7. Add one Phosphorous Reagent #3 Tablet (5157). Cap and swirl until the tablet disintegrates. 8. Remove cap. Hold tube one half inch above the white area of the Phosphorous Color Chart (1312). Looking down through the solution, match sample color to a color standard. Record as lbs/acre phosphorous. The color comparison should be down in natural light. Group Six pH and Potassium Test for pH 1. Fill test tube (0845) to line with Tricon Flocculating Solution (5941).
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5
Acton-Boxborough Regional High School
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ANALYSIS
1. 2. 8. 9. 5. 6. 7. 8. (2 points) Complete the data table using class data. (1 point) Where was sample 1 from? Explain your choice using specific references to your data. (1 point) Where was sample 2 from? Explain your choice using specific references to your data. (1 point) Where was sample 3 from? Explain your choice using specific references to your data. (1 point) Where was sample 4 from? Explain your choice using specific references to your data. (1 point) Where was sample 5 from? Explain your choice using specific references to your data. (2 points) Identify and explain the relationship between porosity and soil texture? (2 points) Which of the soils would be most suitable for growing a field of corn which grows best at a pH of 6.0 7.0 and is known to be a heavy nitrogen consumer with moderate potassium and phosphorous needs? Explain your answer. (2 points) Which of the soils would be most suitable for lining a landfill? Explain your answer.
9.
10. (2 points) Which of the soils would be most suitable for siting a septic tank and leaching field? Explain your answer.
SOURCES
This lab was modified from a version provided at the 2000 AP Environmental Science workshop series sponsored by the College Board and excerpted from the Wards lab titled Exploring Porosity and Permeability, and the LaMotte Soil Handbook.
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PRE-LAB QUESTIONS
Questions are one point each. Questions may be answered in the space provided or on a separate sheet. 1. Complete the table below.
____%Sand, ____%Silt, ____%Clay ____%Sand, ____%Silt, ____%Clay ____%Sand, ____%Silt, ____%Clay ____%Sand, ____%Silt, ____%Clay
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9.
What are the three most important plant nutrients? Briefly describe the importance of each in plant growth.
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10. What is the ideal pH for most soils? Why is this the ideal pH?