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ISSN 10227954, Russian Journal of Genetics, 2010, Vol. 46, No. 9, pp. 1062–1066. © Pleiades Publishing, Inc., 2010.

Original Russian Text © B.F. Chadov, N.B. Fedorova, E.V. Chadova, E.A. Khotskina, M.P. Moshkin, D.V. Petrovski, 2010, published in Genetika, 2010, Vol. 46, No. 9, pp. 1196–1201.

Genetic Mutation Affects the Energy Status of Drosophila


B. F. Chadova, N. B. Fedorovaa, E. V. Chadovaa, E. A. Khotskinaa,
M. P. Moshkina,b, and D. V. Petrovskib
a
Research Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk, 630090Russia
email: chadov@bionet.nsc.ru
b
Research Institute of Systematics and Animal Ecology, Russian Academy of Sciences, Novosibirsk, 630005 Russia
Received January 28, 2010

Abstract—Conditional dominant lethals (CDL) represent a special class of genetic mutations observed in
Drosophila. Mutation manifests as a dominant lethal in one genotype, but lethality is not expressed in another
genotype. CDL mutants exhibit a set of traits discriminating them from classic mutations. We observed
unusually high mobility of flies and high sexual activity of males carrying these mutations. We used special
tests for evaluation of energy metabolism of CDL mutants. Indirect calorimetry (CO2 excretion measure
ment) has been used for estimation of energy exchange in four mutant and two control fly lines. A special
device has been used for evaluation of locomotor activity of these fly lines. Energy exchange and locomotor
activity in CDL mutants were significantly higher than in control lines. We conclude that some genetic muta
tions are capable of increasing energy dissipation in their carriers.
DOI: 10.1134/S1022795410090127

INTRODUCTION protocols permitted to produce large numbers of


CDLs including those whose manifestation were not
The genetic material is organized in chromosomes.
associated with sex [3]. Gradually, the general view of
Chromosomes’ functions are conservation, transmis
these mutations was developed and CDL were as
sion, and manifestation of the genetic information.
attributed to a special mutation class [6].
The definition of the chromosome function is
acknowledged but incomplete. A special process of Originally, CDL were distinguished by two types of
energy circulation underlying the basis of life is hidden manifestation. These were both recessive and domi
by manifestation of genetic information. “Energy cap nant (in case of some genotypes) mutations. CDL
ture and retention in the infinite sequence of cyclic could lose dominant lethal manifestation in case of
and quasicyclic chemical reactions” were designated chromosomal rearrangements [7]. Mutation manifes
as the essence of life [1]. Energy aspect in genetic sys tation was accompanied by a parental effect [3, 7]. The
tem function is revealed in examination of a special mutant offspring often showed nonsymmetrical distur
mutation class, conditional dominant lethals. bances in development (morphoses) [3, 8] (Fig. 1).
Depending on the line and type of cross, the propor
tion of the offspring with morphoses varied from sev
CONDITIONAL DOMINANT LETHALS: eral percent to tens of percents [9]. The capacity of the
RECOGNITION AND UNUSUAL PROPERTIES mutants to form morphoses and conditional manifes
The existence of a special mutation class (condi tation of mutations suggested that the genes revealed
tional dominant lethals, CDL) was suggested in 2000, are regulatory and control ontogeny [4, 8]. In addition
when the first CDL in the X chromosome were identi the studies of CDL cultures have shown that CDL
fied in Drosophila melanogaster [2]. It was thought that produced genomic instability [9, 10].
selection of this class of mutations would enable to
reveal genes responsible for traits of intraspecies simi MUTATION LOCALIZATION
larity [2]. Mutations were induced by γirradiation. A
new approach to mutant selection has been employed: In the three out of four proposed protocols for
the mutation should be manifested differently in the CDL identification, the mutation could be exactly
individuals of the same species, it should be lethal in located on the chromosome already in the course of its
the carriers of one genotype and nonlethal in the car selection. Later the mutation is maintained as a reces
riers of another genotype [2]. Drosophila was used in sive lethal in the heterozygote with the corresponding
the development of several protocols for mutation inverted chromosome. The fourth protocol involves
generation in X chromosomes and the chromosomes selection of the CDLcarrying proband by the pres
of the second and the third pairs [3, 4]. In contrast to ence of a morphose. After proband formation the
accidental CDL findings [5], the involvement of new mutation is located in one of the chromosome pairs

1062
GENETIC MUTATION AFFECTS THE ENERGY STATUS 1063

using the lines In(l)Muller5/+, In(2LR)Cy/+, and flies were placed into the cells of the monitor so that an
In(3LR)D/+. infra red ray generated by the device passed the tube in
Mutation localization at the exact region of a poly the center. The time the fly crossed the ray was auto
tene chromosome is problematic as it is conditionally matically registered as an elementary activity act. The
lethal. A lack of lethality manifestation (is the event data on the activity of each the examined fly was sum
taken into accounted during localization) could result marized by the software installed in the device during
either from the absence of the lethal in the region or the designated time period. In our experiment, the
from genotype substitution in the examined descen time interval was 1 min.
dant. The localization is possible if the lethal mutation We assessed the activity of 16 males of line 61, 12 of
has in addition to lethal effect, a visible mutant pheno line 62, 30 of line 7, 17 of line 46, 25 of line 101, and
type. For example, mapped to region 89 on chromo 29 of line 103. All the males were 3 days old. The activ
some 3 the dimorphic “short legged” mutation, which ity was registered during 10 days. For statistical analy
exhibited a visible mutant manifestation several gener sis the data obtained after threeday adaptation of
ations after its appearance. males in the tubes were used.
Localization of four mutations with visible mani The activity data averaged for each line were pre
festation to autosome 2 is currently in progress. Dele sented as hour plot obtained after processing the pri
tion mapping of one of the mutations ( “Small barrel” mary measurements using the method of sliding mean
phenotype) produced an unexpected result. In a series with a step length of 60 (Fig. 2). The flies of the control
of crosse with 105 deletions on chromosome 2, we and the mutant lines have shown typical day activity
found three regions where the mutation was lethal. rhythms for Drosophila with activity maximum at day
One region is located on the left arm of autosome 2 hours. The peak activity of mutant lines (Fig. 2) signif
while the other two are sited on the right arm of the icantly exceeded that in control lines (F5,123 = 6.9986,
same chromosome. The length of the left arm interact р < 0.001). The average day activity in mutant line was
ing region was from 35B46 to 35B10C1. At the right also higher compared to controls (Fig. 3, left panel).
arm (54–55) seven deletions interact with the muta
tion inducing death of the descendents. These dele Energy turnover. The method of indirect calorime
tions form two distinct interacting loci, 54B1017 and try has been employed in the study, which is based on
55A155B57. This demonstrates an additional prop detection of СО2 release intensity during respiration.
erty of CDL: being recessive in the heterozygote CDL For this purpose the Drosophila males (10 individuals
are capable of cisinteracting (lethal effect) with other from each line) were placed into a medical syringe
recessives in the same heterozygote (deletions). with a ground–in piston with a stopper, where they
were maintained for 1 h. The syringes were prelimi
nary tested for the capacity to keep the carbon dioxide
ENERGY CHANGE and later only the syringe models were used with СО2
Extremely high mutant activity has been noted as loss lower than the measurement error. The volume of
an unusual property of CDL. It was expressed as a high the closed space was 6 ml. After 1 h 5 ml of the air mix
speed of fly movement in the vial and high male mat ture was sent the to an infrared gas analyzer PGA12
ing activity [2, 11]. It appeared that CDL changes the (KOSTIP, Russia), which measured СО2 concentra
energy of fly. tion with precision up to 0.01%. After that the males
Our aim was studying changes in energy metabo were etherized (aether pronarcosi stabilisatum
lism and locomotor activity of Drosophila in response P74/231/1) and weighted with precision of 1 mg. The
to CDL formation. The tested lines (7, 46, 101, and initial level of СО2 in the syringe was measured prior
103) were produced by γ irradiation of laboratory wild the fly exposure. The amount of the released СО2
lines Berlin wild (line 61) and CantonS (line 62). The (VCO2), ml/g h was calculated as the difference in СО2
latter served as control. Males from the mutant and concentration before and after the experiment.
control lines possessed similar phenotype and The experiment was conducted using the lines
appeared normal. Both control and mutant Drosophila developed under the same conditions. The total num
lines were maintained on standard medium at 24°С. bers of measurements were 27 in line 61, 21 in line 62,
The same temperature was used during measurements 28 in line 7, 26 in line 46, 23 in line 101, and 29 in line
of movement activity and energy metabolism. 103. We used oneway analysis of variance and multi
Locomotor activity. Locomotor activity of the adult ple comparisons of means based on LSDtest.
Drosophila was measured using Drosophila movement The lowest СО2 release was observed in flies of con
activity device (Drosophila Monitor Activity, Model trol line 61 and the maximum one, in mutant lines 46
DAM 2, TriKinetics, United States), which permits to and 101 (Fig. 3, right panel). CO2 release in four
register simultaneously the activity of 32 individuals. mutant lines significantly exceeded that of control line
Each fly was placed into a glass tube 65 mm long and 61 while the release in three mutant lines also
4 mm in diameter with nutrient medium at one end. exceeded that in both control lines 61 and 62. Thus,
The other end of the tube was sealed with cotton stop mutant males overperformed control males in loco
per, which did not prevent air exchange. Tubes with motor activity and energy turnover. Our initial obser

RUSSIAN JOURNAL OF GENETICS Vol. 46 No. 9 2010


1064 CHADOV et al.

(a) (b)

(c) (d)

(e) (f)

(g) (h)

RUSSIAN JOURNAL OF GENETICS Vol. 46 No. 9 2010


GENETIC MUTATION AFFECTS THE ENERGY STATUS 1065

Fig. 1. Morphoses in lines carrying dominant lethals. (a,b) two variants of a smallersize head replacing an eye; (c) two heads on
one neck; (d) doubled exterior male genitals; (e) abdomen rotation at 180°; (f) eye bifurcation; (g) tergite fragment on the abdo
men; (h) the lack of two tergites on the right.

100

80

60
Activity, acts/h

40

20

0
11 17 23 5 11 17 23 5 11 17 23 5
Time day, hours

Fig. 2. Imago locomotor activity in six lines of Drosophila during three days. Mutant lines: 7 (solid diamond), 46 (solid square),
101 (solid circle), and 103 (solid triangle); control lines: 61 (open circle) and 62 (open triangle). Axe Y, the number of fly crossings
of the tube center.

50 A 7
A A
A
A
CO2 excretion, ml/g h

40 B 6
B, C B
Activity, acts/h

30 B, C 5 C
C
20 4 D

10 3

0 2
61 62 101 103 7 46 61 62 7 103 46 101
Lines

Fig. 3. Day average locomotor activity (left panel) and CO2 excretion (right panel) in control (61, 62) and mutant (7, 46, 101, and
103) fly lines. The letters over bars indicate significantly different average values of activity (F5, 123 = 7. 34, p < 0.001) and CO2
release (F5, 148 = 31.89, р < 0.001). Multiple averages comparison were done using LSDtest.

vation on unusually high activity of mutants was con the most important property. Indeed, the genome is an
firmed. instrument responsible for energy dissipation from the
external source in the systems operating with cyclic
The obtained results indicate an increase in energy and quasicyclic chemical reactions [1].
turnover in response to the appearance of definite class
of mutation. These results suggest that in a large pool
of genetic mutations, mutations that increase energy ACKNOWLEDGMENTS
dissipation could exist. These mutations might permit
other mutations to work in the opposite direction, to We thank Prof. John T. Berns from Bethany Col
make the individual more evolutionally complex by lege, United States for the data on chronobiology and
increasing its negenthropy [12]. The role of genetic for assistance in organizing experiments on movement
mutations could be more important than changes in activity of flies.
the mutant phenotype, which geneticists use for muta The study was supported by the Russian Founda
tion recognition and considered them their major and tion for Basic Research (project no. 080400094).

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1066 CHADOV et al.

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