Slide lab review

Tahany M. Abu Al - Teen

Mohamed Tawalbe

Collection of the most important slides in the first 7 labs of oral histology lab

1 2 3 4 5

Primary epithelial band Vestibular lamina Dental lamina Early tooth formation (Bud stage) Condensed ectomesenchymal tissue

*** How to differentiate btwn dental lamina and vestibular lamina in any section??

Dental lamina
Goes lingually Has swelling (condensation) at it's end where the cells are packed together Surrounded by condensed ectomesenchymal tissue Invaginate into condensed ectomesenchymal tissue

Vestibular lamina
Goes buccally Cells have spaces btwn them till the whole structure becomes a space Ectomesenchymal tissue around vestibular lamina doesn't condense Doesn't invaginate

(Primary epithelial band = PEB, Dental lamina = DL, Vestibular lamina = VL) ** Primary epithelial band = invagination of oral epithelium into underlying condensed ectomesenchymal tissue and it divides there into dental and vestibular laminea ** PEB and DL and VL all these exist in condensed ectomesenchymal tissue ** Invagination of DL into condensed ectomesenchymal tissue is responsible for teeth formation and it passes through 3 stages (based on the shape of the invagination) and these stages are : 1. Bud stage 2. Cap stage 3. Bell stage ** In this slide the invagination looks like a bud that's why we call this stage of teeth formation the " Bud stage " SO >> the bud, cap and bell all these come from DL and they describe the way in which crowns (nt roots) are formed

1 DL 2 The cells at the end of DL start to pack together and prepare themselves to invaginate into ectomesenchymal tissue to start teeth formation 3 The ectomesenchymal tissue starts to condense around the end of DL No invagination has appeared yet

Bud Stag e

This slide represents the "Bud Stage" coz the invagination looks like a bud

Cap Stage

3 4

** This slide represents the "Cap stage" coz here the invagination looks like a cap 1 EEE 2 SR 3 IEE 4 Dental papilla 5 Enamel Knot (transient structure in the center of IEE that bulges into dental papilla)

1 SR 2 SI 3 mature IEE (preameloblast) 4 undifferentiated ectomesenchymal cells (are going to become odontoblasts) ** At which stage this slide is?? Notice 1. The presence of SI (2-3 layers of cells over IEE) 2. IEE cells are mature and about to differentiate into ameloblasts 3. The ectomesenchymal cells aren't differentiated yet 4. NO hard tissue formation has started yet All these tell that this tooth is in the "Early Bell Stage" and it "Early tage" isn't in the cap stage coz in cap stage we don't have SI and the IEE cells are slightly columnar, and it isn't in the late bell stage also coz we don't have any hard tissue formation and (dentine + enamel) producing cells aren't mature yet

4 5 6

1 2 3 9 1 2 3 4 5 6 7 8 9 EEE Enamel formation Dentine formation SR IEE Cervical loop Area where there's NO hard tissue formation Dental follicle Dental papilla 7

** At which stage this slide is?? It is at "Late Bell Stage" coz we have hard tissue formation

4 3

1 2 3 4 5

EEE SR Enamel Dentine Dental pulp

This tooth has 2 cusps

Notice these lines that are running obliquely across the prisms of enamel these are the long incremental lines or enamel striae (nt all of them reach the surface) When enamel striae appear like lines running obliquely across enamel prisms then this is a longitudinal section across enamel

The previous slide represents Enamel lamella than runs through the entire thickness of enamel

Enamel tufts

Enamel spindles

Scalloped pattern of EDJ This slide represents two structures exist at EDJ and these are Enamel tufts and Enamel spindles ** How to differentiate btwn them in a histological section?? ** Enamel spindles = are of unequal length and aren't backed together and thicker than enamel tufts and they are believed to be odontoblastic processes ** Enamel tufts = resemble tuft of grass, are approximately of equal length, very close to each other and backed together and thinner than enamel spindles

The encircled structure represents enamel prisms

The previous slide represents enamel striae but this is a cross section of enamel that's why they appear circumpulpally running

The encircled structure here shows dead tracts of dentine (dead odontoblastic processes)

1 2 3 4

SR SI Ameloblasts Enamel

The encircled structures are Tome's processes ** At which stage of amelogenesis this stage is?? Secretory stage

1 2 3 4 5 6 7 8

undifferentiated ectomesenchymal cells (dental papilla) Odontoblast Dentine Enamel Ameloblast Tome's process SI SR

PDL

A = Granular layer of Tome's B = Cementum C = Hyaline layer (appears as space) Arrow head = circumpulpal dentine Is it coronal or root dentine?? Root dentine (based on the type of the layers)

A = Peritubular Dentine (around dentinal tubules) B = Intertubular Dentine (btwn neighboring dentinal tubules) Arrow head = Dentinal tubules

Mineralized collagenous matrix

The previous slides represent dentinal tubules that are impeded in mineralized collagenous matrix Vital dentinal tubules contain odontoblastic processes and dentinal fluid Unvital dentinal tubules are called dead tracts (coz odontoblastic processes inside them become dead)

Here dentinal tubules appear as real tubules coz this is a longitudinal section across dentine

In the previous slide dentinal tubules appear as circles coz this is a cross section across dentine

Notice here odontoblastic processes inside dentinal tubules (dark pink color)

In the previous slide notice the amount of branching of dentinal tubules and odontoblastic processes inside them Which layer of dentine is this?? Mantle dentine (1st layer of dentine in the crown) How did I know?? the presence of a jxn which is scalloped in pattern and it is the one that separate btwn enamel and dentine that's why this layer of dentine is in the crown and because it has too many branching it is mantle dentine Notice also that in mantle dentine collagen fibers are parallel to EDJ

A = Circumpulpal dentine in the crown B = Circumpulpal dentine in the root In both (crown and root) circumpulpal dentine is running circumpulpally (around the pulp)

Arrow head = Predentine

A = Shreger line B = contour lines of Owen

C = long period incremental lines of dentine (don't think that these are dead tracts coz as you notice they are of unequal length and don't extend through the entire thickness of dentine)

This slide shows the dead tracts, notice how they are of equal length and of approximate distances from each other and how they extend through the entire thickness of dentine and they are dead coz odontoblastic processes were killed by a stimulus

1 2 3/ 3/

This is a slide showing different layers of dental pulp 1 Supraodontoblastic layer 2 Odontoblastic layer 3 Subodontoblastic layer a. Cell free zone of Weil b. Cell rich zone 4 Core of dental pulp

1 2 3 4

This is a clearer slide showing dentine and dental pulp 1 2 3 4 Odontoblastic layer Cell free zone of Weil Cell rich zone Predentine 4 3 2 1

This slide represents unerupting tooth 1 Reduced enamel epithelium (exists on the top surface of enamel as long as the tooth isn't erupting yet but once it erupts into the mouth it disappears and appears to cover the cervical part of enamel only but not the surface) 2 Enamel (mature, appears as space) 3 Dentine 4 Dental pulp 1 2 3

This slide represents the apical part of the root 1 Root diaphragm (the only region at which the epithelial root sheath cells don't disintegrate) 2 Proliferation zone of Dental papilla 3 Proliferation zone of Dental follicle

3 1 2 3 Epithelial rests of malassayz (remnants of ERS) Root dentine Odontoblasts

Arrow heads = Epithelial rests of malassayz (aggregation of cells in the PDL)

1st root

2nd root

** How many roots is this tooth going to have?? 2 roots

2 5

1 Dentine 2 Predentine 3 Cementum 4 Cementoblasts 5 Epithelial rests of malassayz 6 PDL 7 Blood vessels 8 is it a blood vessel?? Of course not it is an osteon (building unit of bone) 9 Alveolar bone

Arrow head = Cellular cementum 2 Arrow heads = cementocytes

Arrow heads = Incremental lines of cementum

The encircled structure shows odontoclasts than are eating the root of the deciduous tooth

Thank you all and good luck in your final exams Done by: Tahany M. Abu Al - Teen