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1. The nucleotide genome consists of the sequence of nucleic acid that encodes genetic information on DNA.

(a) True (b) False 2. What enables scientists to take nucleotide fragments from other DNA and reassemble fragments into a new nucleotide sequence? (a) Enzyme DNA technology (b) Enzyme technology (c) Recombinant DNA technology (d) Recombinant enzyme technology 3. What is used to cut DNA double-helix strand DNA along the exterior of the strand? (a) Overhang (b) Restriction enzymes (c) Restriction fragment (d) Recognition sequence 4. What is the particular nucleotide sequence of a double-helical segment called? (a) Overhang (b) Restriction enzymes (c) Restriction fragment (d) Recognition sequence 5. What is the end (a) Overhang (b) Restriction enzymes (c) Restriction fragment (d) Recognition sequence of the cut of a double-helical segment called?

6. What results when two incisions are made in a double-helical segment? (a) Overhang (b) Restriction enzymes (c) Restriction fragment (d) Recognition sequence 7. The four nucleotides are adenine (A), cytosine (C), guanine (G) and thymine (T). (a) True (b) False

8. What is another (a) Vector (b) Plasmid (c) Restriction endonucleases (d) Agarose gel

name

for

restriction

enzyme?

9. The Southern blotting technique is used for detecting specific restriction fragments. (a) True (b) False 10. Scientists synthesize fragments of DNA and RNA using a process known as polymerase chain reaction (PCR). (a) True (b) False

11) Northen blots probe a) b) c) d) Carbohydrates DNA Proteins RNA

12) Which is the most specific recombinant DNA library? a) b) c) d) Genomic Protein cDNA chromosomal

13) Recombinant DNA research is dependent on ________ a) b) selectively replicates RNA probes and selectively replicates DNA

c) d)

selectively replicates DNA probes DNA

14) A YAC is a useful __________. a) b) c) d) Vector Screen Host probe

15) The polymerase chain reaction is a technique that __________. a) b) c) d) selectively replicates RNA probes and selectively replicates DNA selectively replicates DNA probes DNA

16) Chromosome walking is a technique used to __________. a) b) to another. c) d) move chromosomes around the nucleus move a fragment of chromosomal DNA from one area of a chromosome

recombination between chromosomal DNA of two different species a method used to locate a gene using a set of clones from a DNA library

17) Genetic engineering is the manipulation of ________ for practical purpose.


Genetic bacteria Genetic plant Genetic material

Genetic animal technology.

18) The ________ technology has launched an industrial revolution in bio-

DNA transfer DNA recombinant genetic bacteria genetic transferring

19) ________ was the first ever cloned animal.


Dolly Tomy Kitty Spike

20) Gene is made up of ________.


Proteins RNA DNA All of them mankind.

21) ________ is defined as the use of living organisms for the welfare of

Micro-biology Human biology Biotechnology Zoology

22) A gene is inserted into a DNA molecule called ________.


vector plasmid both none of them

23) ________ are small, extra circular DNA molecules found in some bacteria.

vector plasmid chromosome genetic engineered DNA

24) Plasmid replicated ________ of the host cell.


dependent independent mostly dependent mostly independent

25) Plasmids are generally found in ________.


bacteria vertebrates all living organisms bacteriophages ________.

26) The group of enzymes that are used to cut up the DNA molecule are called

restriction enzymes DNA ligase enzyme DNA polymerase enzyme none of them

27) The molecular scissors in the bacterial cell are ________.


DNA ligase enzyme vector plasmid restriction enzyme

28) ________ are taken as host in DNA recombinant technology.


bacterial cells vectors plasmids

bacteriophage

29) The gene carried by recombinant molecule is called ________.


cloned copied multiplied engineered

30) When a foreign gene is inserted to natural plants they are called ________.

Transgenic plants genetically engineered plants both A and B all of them

31) In plant cell the rDNA can be introduced into ________.


Embryo Protoplast Cell having cell wall Both A and B

32) The only plasmid for transgenic plants is ________.


Pr-plasmid Ti-plasmid Ds-plasmid All of them

33) ________ is the host for Ti-plasmid.


Bacteriophage Agrobacterium Phenylalamine All bacteria ________.

34) The difference in DNA electrophoresis patterns among individuals is called

DNA finger prints DNA fragment length DNA length measurement Restriction fragment length polymorphism

35) To produce a DNA finger print ________ would suffice.


A sample of blood A sample of saliva Sample of hair folicle All of them

36) Human Genome Project began in ________.


1990 1980 1994 1991 ________.

37) The cell which contains all the genetic potential of the organism is called

Potitotent Totipotent Sturgid call Complete cell

38) The copies of individuals formed by clonig are called ________.


Twins Identicals Clones Copies