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Determine % viability = 113 =113 = 0.95 x100=95% 113+6 119 Determine cells/ml = 113 = 28 4 28 x 2 x 10,000 = 560,000 = 5.6x105 cells/ml
Dilution made in trypan blue
Determine total cells = 5.6 x 105 cells/ml x 5 mls =2.8 x106 cells/ml Note: cells in total of 5 mls when counted 11. Clean the hemocytometer by rinsing with dH2O then 70% IPA and drying thoroughly with a soft wipe.
The Johns Hopkins Medical Institutions The Genetic Resources Core Facility: The Cell Center Blalock 1016/600 N. Wolfe Street Baltimore, Maryland 21287 Director: Margaret B. Penno, Ph.D. Tel: 410-614-0060 E-mail: penno@jhmi.edu
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