HPLC Fenitoina

PHARMACEUTICALS
HPLC Applications
Analgesics
Anti-Inflammatories Anti-Inflammatories
�
8156 � 10158
�
1. Naproxen 1. Fenoprofen
� �
2. Acemetacin 2. Flurbiprofen
3. Indomethacin 3. Ketoprofen
� 4. p-Xylene
� � � �
� � � � � ��
Column: Platinum™ C18, 5µm, 150 x 4.6mm Column: Inertsil® Diol, 5µm, 250 x 4.6mm
(Part No. 32043) (Part No. GL-05646)
Mobile Phase: 0.02M KH2PO4:Methanol:Acetonitrile: Mobile Phase: Hexane:Ethanol (90:10)
pH4.5 (40:50:10) Flow Rate: 1.0mL/min
Flow Rate: 1.0mL/min Detector: UV at 254
Detector: UV at 254nm
Analgesics �
Analgesics
10124 9385
��
��
1. Aspirin 1. Aspirin
�� 2. Acetaminophen � 2. Acetaminophen
3. Naproxen 3. Naproxen
4. Fenprofen 4. Fenoprofen
5. Ibuprofen � �
��
6. Diclofenac
��
� � � � � ��
� � � � � ��
Column: Alltima C8, 5µm, 150 x 4.6mm (Part No. 88072)
™
Column: Prevail™ Amide, 5µm, 150 x 4.6mm
Mobile Phase: A: 0.05M KH2PO4 pH3.3 B: Methanol C: Acetonitrile (Part No. 88660)
Gradient: Time: 0 2 5 Mobile Phase: 0.02M K2HPO4 pH7.0:Acetonitrile (75:25)
%B: 10 10 30 Flow Rate: 1.0mL/min
%C: 10 10 25 Detector: UV at 210nm
Flow Rate: 2.0mL/min
Basic Drugs Naproxen

2 10374 8769
1. Procainimide
2. Acetaminophen CH3
3. Theophylline
1 4. Caffeine
CHCOH
3
4 O
H3CO
0 3 6 9 12 15 Min. � � ��
Column: Alltima™ HP C8, 5µm, 150 x 4.6mm Column: Chirobiotic V™, 250 x 4.6mm (Part No. 11024)
(Part No. 87751) Mobile Phase: 0.1% Triethylammonium Acetate (TEAA),
Mobile Phase: 0.05M Phosphate Buffer pH3.0:Methanol (80:20) pH7:THF (90:10)
Flow Rate: 1.0mL/min Flow Rate: 1.0mL/min
Detector: UV at 254nm Detector: UV at 254nm
© Copyright 2004 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices.
This is a partial listing of our chromatograms. Visit chromatography.com for our

482 complete searchable chromatogram database.
PHARMACEUTICALS
HPLC Applications
Anti-Depressants
Tricyclic Amines Alprazolam and Triazolam by the

3 9105 USP XXII Method 7368
1. Fluphenazine HCL 1. Triazolam

2 2. Alprazolam
2. Chlorpromazine HCL
3. Phenothiazine HCL
1
�
�
0 1 3 2 4 5 6 7 8 Min. � � � � � ��
Column: Alltima™ CN, 3µm, 150 x 4.6mm Column: Alltima™ Silica, 5µm, 300 x 4.6mm (Custom)
(Part No. 81189) Mobile Phase: CH3CN:CHCl3:i-BuOH:Water:Glacial HOAc (85:8:5:2:0.05)
Mobile Phase: 0.01M KH2PO4 pH2.6:Acetonitrile (50:50) Flow Rate: 2.0mL/min
Flow Rate: 1.0mL/min Detector: UV at 254nm
Basic Drugs Tricyclic Antidepressants
10353 10373
1
1. Diphenhydramine 1. Desipramine
2 4
� 2. Nortriptyline 2. Nortryptyline
3. Amitriptyline 3. Diphenhydramine
� 4. Amitriptyline
�
3
� � � � � � � � � �� 0 2 4 6 8 10 12 14 16 18 20 22 Min.
Column: Alltima HP C18 Amide, 5µm, 150 x 4.6mm
™
Column: Alltima™ HP C8, 5µm, 150 x 4.6mm
(Part No. 87733) (Part No. 87751)
Mobile Phase: 0.025M Phosphate Buffer pH7:Methanol (15:85) Mobile Phase: A: 0.025M Phosphate Buffer pH7 B: Methanol
Flow Rate: 1mL/min Gradient: Time 0 8 20
Detector: UV at 254nm %B 60 75 75
Benzodiazepines and Metabolites Universal Combinatorial High Speed

8691 Gradient Application 8957
1. Chlordiazepoxide degradant 5. Chlordiazepoxide 1. Theophylline

2. Nitrazepam 6. Chlordiazepoxide degradant 2. Acetylsalicylic Acid
3. Norchlordiazepoxide 7. Diazepam 3. Propranolol
4. Nordiazepam 4. Nortriptyline
3 4
� 5. Trimipramine
5
�
�
� 2
1
�
�
�
� � � �� 0 1 2 3 4 5 6 Min.
Column: Platinum™ EPS C18, 3µm, 53 x 7mm Rocket™ Column: Alltima™ C18, 3µm, 53 x 7mm Rocket™ (Part No. 50605)
(Part No. 50573) Mobile Phase: A: 0.1% TFA in Water B: 0.1% TFA in Acetonitrile
Mobile Phase: 0.05M Ammonium Acetate pH5.5:Acetonitrile Gradient: Time: 0 6
(65:35) %B: 10 90
Detector: UV at 254nm Detector: ELSD

complete searchable chromatogram database. 483
PHARMACEUTICALS
HPLC Applications
Anti-Asthmatics
Methylxanthines Theophylline & Metabolites
3 8384 8679
1 2 1. Theobromine 1. Xanthine
2. Theophylline 2. Theobromine
3. 8-Chlorotheophylline 3. Theophylline
4 1
5 4. Caffeine 4. Caffeine
5. 7-(2-Chloroethyl) 5. 8-Chlorotheophylline
theophylline 6. 7-(2-chloroethyl)theophylline
2
3 4 5
6
0 2 4 6 8 10 Min. 0 1 2 3 Min.
Column: Platinum™ EPS C18, 100Å, 5µm, 150 x 4.6mm Column: Platinum™ EPS C18, 3µm, 33 x 7mm Rocket™
(Part No. 32214) (Part No. 50575)
Mobile Phase: Water:Acetonitrile:Methanol: Glacial Acetic Acid Mobile Phase: 0.010M Sodium Acetate pH4.0:Methanol (70:30)
(60:10:30:1) Flow Rate: 5.0mL/min
Barbiturates
2 Barbiturate Mix Barbiturate Mix
10375 8684
1. Barbital 1. Barbital
5 2. Phenobarbital 2. Butalbarbital
3. Talbutal 3. Aprobarbital
4. Amobarbital 4. Talbutal
1
5. Mephobarbital 5. Butabarbital
3 8 6. Phenobarbital
1 7. Amobarbital
3 2 8. Mephobarbital
4 5 6
4 7
0 2 4 6 8 10 12 14 16 18 20 Min. 0 1 2 3 4 Min.
Column: Alltima™ HP C8, 5µm, 250 x 4.6mm Column: Platinum™ EPS C18, 3µm, 53 x 7mm Rocket™
(Part No. 87751) (Part No. 50575)
Mobile Phase: 0.05M Ammonium Acetate:Acetonitrile (78:22) Mobile Phase: 0.01M Sodium Acetate pH4.0:Acetonitrile (75:25)
Sedatives Anesthetic
� Sedatives/Hypnotics Anesthetic Mix
8160 � 10118
� 1. Barbital 1. p-Aminobenzoic Acid

2. Phenobarbital 2. Benzocaine
3. Butalbital 3. Tetracaine
�
� 4. Oxazepam 4. Dibucaine
� 5. Triazolam � 5. Butacaine
� �
�
� � � � � ��
Column: Alltima™ C8, 5µm, 150 x 4.6mm (Part No. 88072)

� � � �� Mobile Phase: A: Water B: Methanol C: Acetonitrile
Column: Platinum™ C18, 5µm, 150 x 4.6mm Gradient: Time: 0 7
(Part No. 32043) %B: 15 45
Mobile Phase: 0.05M NH4H2PO4 pH 4.5 Methanol:Acetonitrile (40:50:10) %C: 15 35

PHARMACEUTICALS
HPLC Applications
Antihistamines
Antihistamines � Antihistamines
4 7232 10163
��
3 5 1. Tripelennamine 1. Tripelennamine
2. Triprolidine 2. Triprolidine
�
2 3. Cyclizine � 3. Cyclizine
4. Chlorcyclizine 4. Pyrrobutamine Phosphate
1 5. Meclizine � 5. Meclizine
0 5 10 15 20 25 Min. � � � � � ��
Column: Alltima™ C8, 5µm, 250 x 4.6mm (Part No. 88076) Column: ZirChrom® PS, 3µm, 50 x 4.6mm (Part No. ZR09-0546)
Mobile Phase: A: 0.05M Potassium Phosphate, pH3.2 Mobile Phase: A: Acetonitrile
B: Acetonitrile B: 25mM Hydrochloric Acid
Gradient: Time: 0 5 20 25 Gradient: Time: 0 1.0 1.1 4.0
%B: 25 25 70 70 %B: 95 85 80 40
Detector: UV at 220nm Detector: UV at 254
� Very Polar Bases Basic Antihistamines at High pH

10169 7 10174
4
1. Maleate 1. Maleate
� 2. Phenylephrine 2. Scopolamine
3. Phenylpropanolamine 3 3. Pseudoephedrine
4. Pyrilamine 5 4. Doxylamine
� 5. Chlorpheniramine 1 5. Chlorpheniramine
6. Triprolidine
� � 2 7. Diphenhydramine
6
� � � � � �� 0 5 10 Min.
Column: ZORBAX® SB-Aq, 5µm, 150 x 4.6mm Column: ZORBAX® Extend-C18, 5µm, 150 x 4.6mm
(Part No. 883975-914) (Part No. 773450-902)
Mobile Phase: 0.2% TFA:Acetonitrile (90:10) Mobile Phase: 0.02M Triethylamine:Methanol (30:70)
Flow Rate: 1.5 mL/min Flow Rate: 1.0mL/min
Column Temp: 25˚C Detector: UV at 254nm
Basic Compounds in Mid pH Range OTC Antihistamine and Excipients

10179 � 10275
�
1. Doxylamine � 1. Diphenhydramine
2. Chlorpheniramine 2. PEG, Gelatin
3. Triprolidine 3. Sorbitan
� �
4. Glycerol
5. Sorbitol
�
� �
��
��
Column: ZORBAX® Bonus-RP, 5µm, 150 x 4.6mm Column: Prevail Carbohydrate ES, 5µm, 250 x 4.6mm
™
(Part No. 883668-901) (Part No. 35101)

Mobile Phase: 0.025M Ammonium Acetate pH5.5:Acetonitrile (80:20) Mobile Phase: Acetonitrile:0.1% Ammonium Carbonate
Flow Rate: 1.5mL/min pH6.5 (80:20)
Column Temp: 40°C Flow Rate: 1.0mL/min
Detector: UV at 254nm Detector: ELSD

PHARMACEUTICALS
HPLC Applications
Anticonvulsants
Anticonvulsants Flavonoids
9394 10164
�
3
1. Ethotoin 1. Primidone
�
2. Mephenytoin 2. Metharbital
3. Carbamazepine 3. Mephenytoin
4 4. Diazepam 4. Phenobarbital
� �
5. Phenytoin
2
1 �
0 2 4 6 8 Min. � � � � � ��
Column: Prevail™ Phenyl, 5µm, 150 x 4.6mm Column: ZirChrom® DiamondBond™-C18, 3µm, 100 x 4.6mm
(Part No. 99239) (Part No. DB01-1046 )
Mobile Phase: A: Water B: Acetonitrile Mobile Phase: 0.05M Ammonium Phosphate pH7.0:THF (75:25)
Gradient: Time: 0 15 Flow Rate: 1.0mL/min
%B: 25 60 Detector: UV at 220
Detector: UV at 254
Anticonvulsants Anticonvulsants
10348 10349
1. Ethotoin 1. Ethotoin
2. Phenytoin 2. Phenytoin
� 3. Mephenytoin � � 3. Mephenytoin
�
� 4. Carbamazepine 4. Carbamazepine
�
� �
� � � � � � � � � � ��
Column: Alltima™ HP EPS C18, 5µm, 150 x 4.6mm Column: Alltima HP C18 HiLoad, 5µm, 150 x 4.6mm
(Part No. 87715) (Part No. 87697)
Mobile Phase: 0.025M Ammonium Acetate:Acetonitrile (70:30) Mobile Phase: 0.025M Ammonium Acetate:Acetonitrile (70:30)
Detector: ELSD Detector: ELSD
Amphetamines
MDA & MDMA Analogs MDA and MDMA Analogs
10014 1 2 4 10427
�
�
1. 2,3-MDA 3 1. 2,3-MDA
2. 3,4-MDA 2. 3,4-MDA
3. 2,3-MDMA 3. 2,3-MDMA
4. 3,4-MDMA 4. 3,4-MDMA
� �
� � � � �� 0 1 2 3 4 5 6 7 8 9 Min.
Column: Alltima™ Silica, 3µm, 100 x 4.6mm (Part No. 81404) Column: Alltima HP Silica, 3µm, 150 x 4.6mm (Part No. 87786)
Mobile Phase: 1%NH4OH in Methanol:Acetonitrile (25:75) Mobile Phase: 1%NH4OH in Methanol:Acetonitrile(75:25)
Detector: UV at 280nm Column Temp: 25°C

PHARMACEUTICALS
HPLC Applications
Anti-Hypertensives
Antihypertensives Basic Drugs
9395 10161
3
�
1. Metoprolol 1. Labetalol
�
2. Oxprenolol 2. Acebutolol
1 2 3. Propranolol 3. Oxprenolol
4. Lidocaine
� � �
5. Alprenolol
6. Propranolol
0 1 2 Min. � � � � � ��
Column: Prevail C18, 3µm, 53 x 7mm Part No. (99279)
™
Column: ZirChrom PBD, 3µm, 150 x 4.6mm
®
Mobile Phase: 0.025M KH2PO4, pH3.0:Acetonitrile (70:30) (Part No. ZR03-1546)

Flow Rate: 4.0mL/min Mobile Phase: 0.01M Triethylamine, 0.05M Tetramethylammonium
Detector: UV at 220 Hydroxide pH 12.2:Acetonitrile (65:35)
Detection: UV at 254
Calcium Channel Blockers Drug Mix

1 10356 10354
1. Diltiazem 1. Codeine
2. Verapamil � 2. Strychnine
4 3. Nicardipine � 3. Quinine
3 4. Nifedipine 4. Quinidine
2
�
�
0 1 2 3 4 5 6 7 8 9 10 Min. � � � � � ��
Column: Alltima™ HP CN, 5µm, 150 x 4.6mm Column: Alltima™ HP C18 Amide, 5µm, 150 x 4.6mm
(Part No. 87769) (Part No. 87733)
Mobile Phase 0.02M KH2PO4 pH3.0:Acetonitrile (70:30) Mobile Phase: 0.025M Potassium Phosphate pH 2.8:Methanol (90:10)
� Opioids Morphines & Metabolites

10165 9150
1. Naloxone �� 1. Normorphine
2. Codeine 2. Morphine-3-Glucuronide
3. Ethylmorphine 3. Morphine-6-Glucuronide
4. Oxycodone 4. Morphine
� � 5. Codeine
�
� �
�
� � � � � ��
Column: ZirChrom DiamondBond-C18, 3µm, 100 x 4.6mm Column: Hypercarb®, 7µm, 100 x 4.6mm
(Part No. DB01-1046) (Part No. 59864750)
Mobile Phase: 0.02M Ammonium Phosphate pH11.0: Mobile Phase: Ammonium Acetate pH 9.0:Methanol (40:60)
Tetrahydrofuran (73.5:26.5) Flow Rate: 1.0mL/min
Detector: UV at 220

PHARMACEUTICALS
HPLC Applications
Steroids
1
Steroids Steroids
9392 10155
2
1. Hydrocortisone 1. Progesterone
3 2. Testosterone 2. Corticosterone
3. Progesterone � 3. Cortisone
4. Prednisone
5. Prednisolone
� �
�
�
0 1 Min. � ��
Column: Prevail™ C18, 3µm, 53 x 7mm Rocket™ Column: Inertsil® Diol, 5µm, 150 x 4.6mm
(Part No. 99279) (Part No. GL-05645)
Mobile Phase: Water:Methanol (10:90) Mobile Phase: Hexane:Ethanol (85:15)
Detector: UV at 254 Column Temp: 40°C
Detector: UV at 254
Hormones Corticosteroids
10145 10391
� 1. β-Trenbolone 1 1. Deoxycorticosterone
2. α-Trenbolone 2 Acetate
3. β-Zearalenol 5 7 2. Hydrocortisone Acetate
4. Testosterone 3. Cortisone Acetate
5. Zeranol 4. Cortisone
� 6. α-Zearalenol 3 4 6 5. Prednisone
��
�� 7. α-Estradiol 6. Hydrocortisone
�
8. Zearalenone 7. Prednisolone
�
9. Diethylstilbestrol
0 1 2 3 4 5 6 7 8 9 10 11 12 13 Min.
� ��
Column: Inertsil® ODS-3, 5µm, 150 x 4.6mm Column: Alltima™ HP CN, 5µm, 150 x 4.6mm
(Part No. GL-01731) (Part No. 87769)
Mobile Phase: Water:Acetonitrile:THF(57:35:8) Mobile Phase: Hexane:Isopropanol (93.7:6.3)
Flow Rate: 1.0mL/min Flow Rate: 2.0 mL/min
Column Temp: 40°C Column Temp: 35°C
Detector: UV at 230 Detector: UV at 254nm
Hydrocortisone Acetate Cream Estropipate Sample

� 9463 10423
1. Methyl Paraben 1. Estrone Sulfate

2. Propyl Paraben 2. Piperazine
� 3. Hydrocortisone Acetate
�
�
��
� � � � � ��
Column: Prevail C18, 5µm, 150 x 4.6mm (Part No. 99208)
™
Column: Alltima™ CN, 5µm, 250 x 4.6mm
Mobile Phase: Water:Acetonitrile (10:90) (Part No. 88189)
Flow Rate: 1.0mL/min Mobile Phase: 0.15% HNO3:Acetonitrile (5:95)
Detector: UV at 254 Flow Rate: 0.5mL/min
Detector: ELSD

PHARMACEUTICALS
HPLC Applications
12
Diuretics Diuretics and Uricosuric Agents
2 15 1. Amiloride HCl Hydrate
4 10246
2. Acetazolamide
1
11 3. Hydrochlorothiazide
4. Triamterene
10
5. Chlorthalidone
13 6. Furosemide
7. Hydroflumethiazide
3 7
8. Clopamide
6 8 9 14 9. Ethacrynic Acid
5
10. Trichloromethiazide
11. Probenecid
12. Indapamide
13. Bumetanide
0 5 10 15 20 25 30 Min. 14. Bendroflumethiazide
15. Spironolactone
Mobile Phase: A: 0.1% TFA in 0.025M Ammonium Acetate B: 0.1% TFA in Acetonitrile
Gradient: Time 0 30
%B 25 65 11
Detector: UV at 254nm 10 Doping Agents
8 1. Amiloride HCl Hydrate
10262
2. Acetazolamide
3. Hydrochlorothiazide
4. Chlorthalidone
12
6. Clopamide
9 7. Trichloromethiazide
4 6 8. Indapamide
7 9. Bendroflumethiazide
5 10. Bumetanide
1 11. Probenecid
2 3 12. Spironolactone
0 1 2 3 4 5 6 7 8 9 10 11 12 Min.
Gradient: Time 0 10 15
%B 25 95 95 3 7
Flow Rate: 1.0 mL/min
Detector: ELSD
8 Diuretics - High Throughput

1. Acetazolamide
10265
1 9 2. Hydrochlorothiazide
3. Triamterene
5. Clopamide
5 6
6. Trichloromethiazide
7. Indapamide
8. Bumetanide
2 4 9. Spironolactone
0 1 2 3 4 Min.
Column: Alltima™ C18, 3µm, 53 x 7mm Rocket™ (Part No. 50605)
Gradient: Time 0 4
%B 20 95

PHARMACEUTICALS
HPLC Applications
Antibiotics
Antibacterials Antibacterials
8711 8764
3
1. Sulfanilimide 1. Oxytetracycline
2. Cefaclor 1 Dihydrate
� � � 3. Cefatrizine 2 2. Tetracycline HCL
4. Thiamphenicol 3. Meclocycline
� 5. Cefotaxime Sulfosalicylate Salt
�
� � � � � �� 0 2 4 6 8 10 Min.
Column: Platinum™ EPS C18, 5µm, 150 x 4.6mm Column: Alltima™ C18, 3µm, 100 x 4.6mm (Part No. 81382)
(Part No. 32043) Mobile Phase: A: 0.05M KH2PO4 pH 3.0 B: Acetonitrile
Mobile Phase: 0.025M KH2PO4 pH3.0:Acetonitrile (90:10) Gradient Time: 0 10
Flow Rate: 1.0mL/min %B: 15 40
Detector: UV at 230nm Flow Rate: 1.0mL/min
Aminoglycoside Antibiotics Antibacterials

9210 9396
3
�
1. Streptomycin 1. Neomycin
2. Amikacin 2. Amoxicillin
3. Tobramycin 3. Vancomycin
4. Neomycin 6 11 4. Cefatrizine
4 9
5. Metampacillin
� � 2
6. Cefotaxime
� 5 7 8 10 7. Unknown
8. Bacitracin
1 9. Pipericillin
10. Bacampacillin
11. Oxacillin
� � � � � � � � � ��
0 2 4 6 8 10 12 14 16 18 20 22 Min.

™
Column: Prevail™ C18, 5µm, 150 x 4.6mm (Part No. 99208)
Mobile Phase: 0.3% Pentafluoropropionic Acid in 43.7mM Ammonium Mobile Phase: A: 0.025M KH2PO4 pH3.0 B: Acetonitrile
Formate pH2.7:0.3% Pentafluoropropionic Acid in Gradient: Time: 0 15 22
Methanol (45:55) %B: 10 40 40
Detector: ELSD Detector: UV at 210
Tylosin and Monensin Sulfa Drugs

10319 10351
� 1. Tylosin (400ppm) 1. Sulfanilamide

2. Monensin (180ppm) � 2. Sulfamerazine
3. Sulfamethazine
4. Sulfamethizole
�
� � �
� � � � � ��
Column: Prevail™ Select C18, 5µm, 150 x 4.6mm Column: Alltima™ HP C18 HiLoad, 5µm, 150 x 4.6mm
(Part No. 99300) (Part No. 87697)
Mobile Phase: Water pH9 w/NH4OH:Acetonitrile (5:95) Mobile Phase: 0.1% Phosphoric Acid:Acetonitrile (85:15)
Detector: ELSD Detector: UV at 254nm

PHARMACEUTICALS
HPLC Applications
Cephalosporins � Tetracyclines
10180 10216
1. Cephalexin 1. Uracil
2. Cephaclor � 2. Minocycline
mAu 1 3. Cephuroxime 3. Tetracycline
�
15 4. Cephoxtin 4. Chlortetracycline
� 5. Doxycycline
10 3
5 2 4 �
0
0 2 4 6 8 10 Min. � � ��
Column: ZORBAX® Bonus-RP, 5µm, 150 x 4.6mm Column: ZORBAX® Rx-C8, 5µm, 150 x 4.6mm
(Part No. 883668-901) (Part No. 883967-901)
Mobile Phase: 0.025M Sodium Citrate pH6:Methanol (75:25) Mobile Phase: 0.1% TFA:Acetonitrile (75:25)
Detector: UV at 254nm Detector: UV 254nm
Antiseptics
� Antibiotics: High Speed Separation Highly Polar Cosmetic Ingredients
10218 10166
�
1. Ceftazidime � 1. Allantoin
2. Cefotaxime 2. Bronopol
� 3. Ciprofloxacin
4. Ceftazolin
�
�
� ��
Column: ZORBAX® SB-C8, 3.5µm, 75 x 4.6 mm Column: ZirChrom® CARB, 3µm, 100 x 4.6mm
(Part No. 866953-906) (Part No. ZR01-1046)
Mobile Phase: 0.1% TFA:Acetonitrile (92:8) Mobile Phase: Water:Acetonitrile (80:20)
Column Temp.: 60˚C Detector: UV at 200
Detector: UV 260nm
Taxols
Taxol® and Related Taxanes in Yew Taxol® and Related Taxanes in Yew
9221 9241
1. 10-Deacetylbaccatin III 1. 10-Deacetylbaccatin III
4. Taxinine M 4. Taxinine M
2. Baccatin III 2. Baccatin III
5. 7-Xylosyl-10-deacetyltaxol 5. 7-Xylosyl-10-deacetyltaxol
3. 7-Xylosyl-10-deacetyltaxol B 3. 7-Xylosyl-10-deacetyltaxol B
6. 7-Xylosyl-10-deacetyltaxol C 6. 7-Xylosyl-10-deacetyltaxol C
� 7. 10-Deacetyltaxol 7. 10-Deacetyltaxol
8. 7-Xylosyltaxol 11 8. 7-Xylosyltaxol
5 10
9. Cephalomannine (Taxol® B) 1 78 13 9. Cephalomannine (Taxol® B)
� 10. 7-Epi-10-deacetyltaxol 10. 7-Epi-10-deacetyltaxol
9
11. Taxol® 11. Taxol®
� 2
12. Taxol® C 4 6 12 12. Taxol® C
�� 13. 7-Epitaxol 13. 7-Epitaxol
� � �� 3
�
��
� � � � � � � � �� 0 1 2 3 4 5 6 7 Min.
Column: Alltima™ CN, 3µm, 53 x 7mm Rocket™ (Part No. 81170)
Column: Alltima™ Phenyl 3µm, 53 x 7mm Rocket™ Mobile Phase: A: 1-Methoxy-2-propanol B: Acetonitrile C: Water
(Part No. 81174) Gradient: Time: 0 8
Mobile Phase: 0.05% TFA in Water:Methanol:Acetonitrile %B: 20 65
(50:19:31) %C: 77 32

NUTRACEUTICALS
HPLC Applications
Ginseng Black Cohosh

Ginsenosides in Korean Ginseng Black Cohosh Extract
10142 9496
� 1. Rh2 6. Rf 1. Cimiracemoside A
� � 2. Rh1 7. Re 2. Actein
� 3. Rg2 8. Rd 2 3. 27-deoxyactein
�
4. Rg3 9. Rc
� 5. Rg1 10. Rb2 3
� ��
11. Rb1
� �� 1
�
� �� 0 10 20 30 40 50 60 Min.
Column: Prevail Carbohydrate ES, 5µm, 250 x 4.6mm
™ Column: Prevail C18, 5µm, 250 x 4.6mm
™
(Part No. 35101) (Part No. 99210)

Mobile Phase: A: Acetonitrile:Water:Isopropyl Alcohol (80:5:15) Mobile Phase: A: 1% Formic Acid B: Acetonitrile
B: Acetonitrile:Water:Isopropyl Alcohol (67:21:12) Gradient: Time: 0 60 65 67 80
Gradient: Time: 0 28 35 45 50 51 57 %B: 20 100 100 20 20
%B: 25 85 80 75 90 100 25 Flow Rate: 1.0mL/min
Flow Rate: 0.8mL/min Detector: ELSD
Detector: ELSD
Ginkgo Biloba
Ginkgo Biloba Flavonol Glycoside Standards Ginkgo Biloba Terpene Lactone Standards
1 4
10239 10287
5
1. Quercetin 1. Bilobalide
2. Kaempferol 2. Ginkgolide J
23
2 3. Isorhamnetin 1 3. Ginkgolide C
4. Ginkgolide A
3 5. Ginkgolide B
0 2 4 6 8 10 12 14 16 18 20 Min. 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Min.
Column: Platinum C18, 5µm, 250 x 4.6mm Column: Alltima™ C18, 3µm, 100 x 4.6mm
(Part No. 32064) (Part No. 813812)
Mobile Phase: A: 0.05% TFA in Water:Methanol (95:5) Mobile Phase: A: 0.1% TFA in Water B: 0.1% TFA in Methanol
B: 0.05% TFA in Methanol Gradient: Time 0 15
Gradient: Time 0 20 %B 10 55
%B 10 90 Flow Rate: 1.3mL/min
Detector: ELSD
Terpene Lactones High Throughput Liquid Ginkgo Biloba Extract Prep
10288 10315
1. Bilobalide 1. Bilobalide
2. Ginkgolide J 2. Ginkgolide J
3. Ginkgolide C 3. Ginkgolide C
4. Ginkgolide A 4. Ginkgolide A
5. Ginkgolide B � 5. Ginkgolide B
4 5
1 23 � �
� �
0 1 2 3 4 5 6 Min. � � ��
Column: Alltima™ C18, 3µm, 53 x 7mm Rocket™ Column: Alltima C18, 5µm, 150 x 10mm
™
(Part No. 50605) (Part No. 81102)

Mobile Phase: A: 0.1% TFA in Water B: 0.1% TFA in Methanol Mobile Phase: A: 0.1% TFA in Water B: 0.1% TFA in Methanol
Gradient: Time 0 5 Gradient: Time 0 45
%B 10 45 %B 22 32

NUTRACEUTICALS
HPLC Applications
Flavonoids
Polyphenolic Flavonoids 1 Flavonoid Mix
9397 2 9271
5
1. Myricetin 1. Myricetin
3
2. Quercetin 2. Quercetin
3. Naringenin 3. Apigenin
1
2 4 4. Hesperetin 4. Kaempferol
6 5. Apigenin 4
6. Kaempferol
3
0 1 2 3 4 5 Min.
0 2 4 6 8 10 12 14 16 18 20 22 24 26 Min.
Column: Prevail™ C18, 5µm, 150 x 4.6mm (Part No. 99208) Column: Alltima™ C18 3µm, 53 x 7mm Rocket™
Mobile Phase: A: 0.025M KH2PO4 pH2.5 B: Acetonitrile (Part No. 50605)
Gradient: Time: 0 15 25 Mobile Phase: 0.1% TFA in Water:0.1% TFA in Acetonitrile
%B: 20 40 40 (65:35)
Detector: UV at 280 Detector: ELSD
Flavonoid Mix Flavonoid Derivatives

8887 10146
�
� 1. Naringin 1. Troxerutin
2. Neohesperidin 2. Rutin
3. Myricetin 12 3. Fisetin
6
4. Quercetin 3 4. Morin
� 5. Naringenin 45 8 5. Luteolin
� � 6. Kaempferol 6. Quercetin
7
� 7. Kaempferol
8. Chrysin
� � �� 0 2 4 6 8 10 12 14 Min.
Column: Alltima™ C18, 3µm, 100 x 4.6mm Column: Inertsil® ODS-3, 5µm, 150 x 4.6mm
(Part No. 81382) (Part No. GL-01731)
Mobile Phase: A: 0.05M KH2PO4 pH 2.5 B: Acetonitrile Mobile Phase: 0.02M NaH2PO4 pH 3.0:Methanol:Tetrahydrofuran
Gradient: Time: 0 15 (50:30:20)
%B: 20 40 Flow Rate: 1.0mL/min
Flow Rate: 1.0mL/min Column Temp: 40°C
Detector: UV at 280nm Detector: UV at 254
St. John’s Wort Echinacea

Hypericins Phenolic Acids
1 9107 3 9110
1. Pseudohypericin 1. Protocatechuic Acid

1
2. Hypericin 2. Chlorogenic Acid
3. Caffeic Acid
2
0 1 2 3 Min. 0 1 2 Min.
Column: Alltima™ C18, 3µm, 53 x 7mm Rocket™ Column: Alltima™ C18, 3µm, 53 x 7mm Rocket™
(Part No. 50605) (Part No. 50605)
Mobile Phase: 0.2% Phosphoric Acid:Methanol (5:95) Mobile Phase: 0.01M K2HPO4 pH2.6:Acetonitrile (80:20)
Detector: Vis at 585nm Detector: UV at 330nm

NUTRACEUTICALS
HPLC Applications
Green Tea
Green Tea Extract - Analytical Green Tea Extract - Preparative
10139 10140
1. Caffeine 1. Caffeine
2. Epicatechin 2. Epicatechin
3. Epigallocatechin Gallate 3. Epigallocatechin Gallate
�
3
�
� 1 2
� � � � � �� 0 2 4 6 8 10 12 14 16 18
Column: Alltima™ C18, 5µm, 150 x 4.6mm (Part No. 88052) Column: Alltima™ C18, 5µm, 150 x 22mm (Part No. 81106)
Mobile Phase: 0.05% TFA:Acetonitrile (87:13) Mobile Phase: 0.05% TFA:Acetonitrile (87:13)
Flow Rate: 1.0mL/min Flow Rate: 23mL/min
Devil’s Claw
Catechins Iridoid Compounds
8825 9191
�
� 1. Gallic Acid 1. 8-para-Coumaroyl Harpagoside
2. Epigallocatechin 2. Harpagoside
3 Catechin
� � 4. Epicatechin
� 5. Epigallocatechin Gallate 2
� 6. Caffeine
7. Gallocatechin
� � 8. Epicatechin Gallate
1
� � � � � �� 0 1 2 3 4 5 6 Min.
Column: Platinum EPS C18, 1.5µm, 33 x 7mm Rocket
™ ™
Column: Alltima C18, 3µm, 53 x 7mm Rocket
™ ™
(Part No. 50577) (Part No. 50605)

Mobile Phase: 0.05% v/v TFA in 87:13 Water:Acetonitrile Mobile Phase: 0.05% TFA:Methanol:Dioxane (75:23:2)
Goldenseal Root Evening Primrose

Alkaloids Triglycerides
9124 10047
1 2
1. Hydrastine � 1. LGG
2. Berberine 2. LGL
3. LLL
4. OGL
5. PGL
6. OLL
� 7. OGO + PLL
��
8. OOO
� ��
0 1 2 Min. � ��
Column: Alltima™ C18, 3µm, 53 x 7mm Rocket™ Column: Alltima™ C18, 5µm, 250 x 4.6mm (Part No. 88056)
(Part No. 50605) Mobile Phase: A: Acetonitrile B: Dichloromethane
Mobile Phase: 0.02M KH2PO4 pH2.6:Methanol (52:48) Gradient: Time: 0 40
Flow Rate: 3.0mL/min %B: 30 50
Detector: ELSD

NUTRACEUTICALS
HPLC Applications
Vitamins
Water- and Fat-Soluble Vitamins Fat-Soluble Vitamins
9545 10279
1. Thiamine 1. Vitamin A (100ng on column)
2. Ascorbic acid 2. Vitamin E (160ng on column)
3. Nicotinic acid 3. Vitamin K1 (130ng on column)
10 4. Niacinamide
56
4 5. Pantothenic acid
�
7 6. Riboflavin
8 7. Cyanocobalamin
8. Biotin �
9 12 14
1 9. Vitamin A
23 11 13 15 �
10. Δ-Tocopherol
11. γ-Tocopherol
12. α-Tocopherol
13. Vitamin D2
0 5 10 15 20 25 30 Min. 14. Vitamin D3 � � � � � � � � � � ��
15. Vitamin K
Column: Prevail™ C18, 5µm, 150 x 4.6mm Column: Alltima™ C18, 3µm, 150 x 1mm
(Part No. 99208) (Part No. 88384)
Mobile Phase: A: Water pH3.2 w/Formic Acid Mobile Phase: Methanol:Chloroform (90:10)
B: Acetonitrile:Methanol (83:17) Flow Rate: 0.05mL/min
Gradient: Time 0 3 10 10.1 30 Detector: ELSD
%B 0 0 45 100 100
Detector: ELSD
Fat-Soluble Vitamins and Tocopherols Vitamin E Soft Gel Tablet

3
9390 9458
2
4 1. Vitamin A 1. Δ-Tocopherol
6
2. Δ-Tocopherol 2. γ-Tocopherol
3. γ-Tocopherol 3. α-Tocopherol
5 4. α-Tocopherol (Vitamin E)
1 5. Vitamin D2
23 7 6. Vitamin D3
7. Vitamin K
1
0 2 4 6 8 10 12 14 Min. 0 3 6 9 12 15 Min.
Column: Prevail® C18, 5µm, 150 x 4.6mm Column: Prevail™ C18, 5µm, 150 x 4.6mm
(Part No. 99208) (Part No. 99208)
Mobile Phase: Acetonitrile:Methanol (75:25) Mobile Phase: Acetonitrile:Methanol (75:25)
Detector: UV at 220 Detector: ELSD
Water-Soluble Vitamins Water-Soluble Vitamins

� �
from Multi-Vitamin Tablet � �
9456 � 8154
1. Thiamine 1. Ascorbic Acid

� � 2. Ascorbic Acid 2. Nicotinic Acid
3. Unknown 3. Pyridoxamine
4. Fumaric Acid � � 4. Niacinamide
� 5. Pyridoxine � 5. Folic Acid
� �
6. Niacinamide 6. Riboflavin
�
7. Pantothenic Acid 7. Vitamin B12
� � � � � ��
Column: Prevail™ C18, 5µm, 150 x 4.6mm Column: Platinum™ C18, 5µm, 150 x 4.6mm (Part No. 32043)
(Part No. 99208) Mobile Phase: A: 0.1M Ammonium Phosphate pH4.5
Mobile Phase: 0.025M KH2PO4 pH3.0:Acetonitrile (97:3) B: Acetonitrile
Flow Rate: 1.0mL/min Gradient: Time: 0 4 15 18
Detector: UV at 212 B%: 5 5 40 40

FOOD & BEVERAGE
HPLC Applications
Carbohydrates
1 Corn Syrup Solids Corn Syrup Oligomers
9354 1 9420
1. Dextrose 5. Maltopentaose 1. Dextrose

2 2. Maltose 6. Maltohexaose 2. Maltose
3 3. Maltotriose 7. Maltoheptaose 3. Maltotriose
4. Maltotetraose 8. Maltooctaose 4. Maltotetraose
2
9. Maltononaose 5. Maltopentaose
10. Maltodecaose 3 6. Maltohexaose
4 5
6 11. Maltoundecaose 7. Maltoheptaose
12. Maltododecaose 8. Maltooctaose
11 13 4 5 6
7 10 12 13. Maltotridecaose
8 9 78
0 5 10 15 20 25 Min. 0 1 2 3 4 5 6 7 8 Min.
Column: Prevail™ Carbohydrate ES, 5µm, 250 x 4.6mm Column: Prevail™ Carbohydrate ES, 5µm, 53 x 7mm Rocket™
(Part No. 35101) (Part No. 35104)
Mobile Phase: A: Acetonitrile B: Water Mobile Phase: A: Acetonitrile B: Water
Gradient: Time 0 15 Gradient: Time: 0 4
%B 35 50 %B:, 32 50
Mono-, Di-, Tri- Saccharides 10009

Sugars 10277
�� 1. iso-Erythritol 9. Maltose 1. Fructose

�� 2. Fructose 10. Raffinose 2. Glucose
3. Sorbitol 11. DP3 3. Sucrose
��
4. Mannitol 12. DP4
� 5. Glucose 13. DP5
6. Inositol 14. DP6
7. Sucrose 15. DP7
�� 8. Maltitol 16. DP8 �
��
� �� 17. DP9
� ��
� �� 18. DP10
� �� 19. DP11
20. DP12
21. DP13
� ��
22. DP14
Column: Prevail Carbohydrate ES, 5µm,
™
Column: Prevail™ Carbohydrate ES, 5µm, 53 x 7mm Rocket™
250 x 4.6mm (Part No. 35101) (Part No. 35104)
Mobile Phase: A: Acetonitrile B: 0.04% Ammonium Hydroxide in Water Mobile Phase: Acetonitrile:Water (75:25)
Gradient: Time: 0 25 40 60 Flow Rate: 2.0mL/min
%B: 17 27 45 55 Column Temp: 30°C
Detector: ELSD
Beer Standards Beer Standards
Unmodified Mobile Phase 9350 1 Modified Mobile Phase 9360
2
2
1 3 1. Fructose 1. Fructose
4 2. Glucose 2. Glucose
3
5 6 3. Sucrose 3. Sucrose
7 4. Maltose 4 4. Maltose
5. Maltotriose 5 6 5. Maltotriose
7
6. Maltotetraose 6. Maltotetraose
7. Maltopentaose 7. Maltopentaose
0 5 10 15 20 25 30 35 40 Min. 0 2 4 6 8 10 12 14 16 18 Min.
Column: Prevail™ Carbohydrate ES, 250 x 4.6mm Column: Prevail™ Carbohydrate ES, 250 x 4.6mm (Part No. 35101)
(Part No. 35101) Mobile Phase: A: Acetonitrile:Acetone (75:25)
Mobile Phase: A: Acetonitrile B: Water B: Water
Gradient: Time: 0 50 Gradient: Time: 0 18
%B: 20 35 %B: 24 50

FOOD & BEVERAGE
HPLC Applications
Sugar Alcohols Microbore Analysis of Glucopyranosides

9352 10310
1. Iso-erythritol 1. n-octyl Glucopyranoside (50ng on column)
1 2 2. Xylitol 2. n-decyl Glucopyranoside (50ng on column)
3. Mannitol 3. n-dodecyl Glucopyranoside (50ng on column)
4. Maltitol
� �
�
3 4
0 2 4 6 8 10 12 Min. � � � � � � � � ��
Column: Prevail™ Carbohydrate ES, 250 x 4.6mm Column: Alltima™ C18, 5µm, 150 x 1mm
(Part No. 35101) (Part No. 88384)
Mobile Phase: Acetonitrile:Water (70:30) Mobile Phase: Methanol:Water (90:10)
3 Imported Ale Wine Analysis - 2000 Sipaska Rosé

9351 � 9474
1. Fructose 1. Fructose
2. Glucose 2. Sorbitol
3. Maltose 3. Glucose
4. Maltotriose 4. Maltose
5. Maltopentaose
6. Maltohexaose
7. Maltoheptaose
4
5 67 �
12 � �
0 5 10 15 20 25 30 35 40 45 50 Min. � � ��
Column: Prevail™ Carbohydrate ES, 5µm, 250 x 4.6mm Column: Prevail™ Carbohydrate ES, 5µm, 250 x 4.6mm
(Part No. 35101) (Part No. 35101)
Mobile Phase: A: Acetonitrile B:Water Mobile Phase: A: Acetonitrile B: Water
%B: 20 35 %B: 17 35
Wine Analysis by Beverage Additives

Ion Exclusion �
5411 9446
1. Citric Acid � 1. Ascorbic Acid

�� 2. Tartaric Acid 2. Saccharin
� � �
3. Glucose 3. Benzoic Acid
4. Malic Acid 4. Aspartame
5. Fructose 5. Vanillin
� � 6. Acetic Acid �
� 7. Glycerol
� �
� 8. Lactic Acid
� �
9. Methanol
10. Ethanol
� ��
Column: IOA-1000, 9mm, 300 x 7.8mm Column: Prevail C18, 3µm, 50 x 2.1mm (Part No.43818)
™
(Part No. 9646) Mobile Phase: Acetonitrile:0.05M Ammonium Acetate pH 5.4, (20:80)
Mobile Phase: 0.005N Sulfuric Acid Flow Rate: 0.3 ml/min
Column Temp: 60°C
Detector: RI

FOOD & BEVERAGE
HPLC Applications
Organic Acids
Organic Acid Mix Rye Bread Extract
� 1. Oxalic Acid 9384 9484
�
2. Tartaric Acid
3. Formic Acid �
�� 4. Malic Acid 1. Tartaric Acid
5. Lactic Acid 2. Malic Acid
6. Acetic Acid 3. Lactic Acid
� � 4. Citric Acid
� 7. Diglycollic Acid
� � 5. Fumaric Acid
� 8. Maleic Acid �
�
9. Citric Acid �
�
�� 10. Succinic Acid
�
11. Fumaric Acid
� � � � � � � � ��
Column: Prevail Organic Acid, 5µm, 150 x 4.6mm

™
™
(Part No. 88640) (Part No. 88640)

Mobile Phase: 0.025M KH2PO4 pH 2.5 Mobile Phase: 0.025M KH2PO4 pH 2.5
5
Underivatized Fatty Acids Organic Acid Mix
9539 3 7 8244
4 1. Lauric Acid 1 1. Tartaric Acid

5 2. Myristic Acid 2. Malic Acid
3. Palmitic Acid 3. Lactic Acid
6 4. Stearic Acid 4. Diglycolic Acid
3
5. Arachidic Acid 5. Maleic Acid
1 6. Behenic Acid 6. Succinic Acid
2 2 7. Fumaric Acid
4 6
0 2 4 6 8 10 12 14 16 18 20 Min. 0 2 4 6 8 10 Min.
Column: Prevail™ Organic Acid, 5µm, 150 x 4.6mm Column: Platinum™ EPS C8, 5µm, 150 x 4.6mm
(Part No. 88640) (Part No. 32420)
Mobile Phase: Acetonitrile:Methanol (75:25) Mobile Phase: 0.025M KH2PO4 pH 2.5:Methanol (97:3)
Detector: ELSD Detector: UV at 220nm
�
��
Short-Chain Aromatic Fatty Acids Using UV and
�
�
Carboxylic Acids 7067 � ELSD in Tandem 10430
1. Oxalic Acid � 1. Caproic Acid 6. Myristic Acid

�� 2. Citric Acid � 2. Caprylic Acid 7. Linoleic Acid
�
� 3. Shikimic Acid � 3. Capric Acid 8. Palmitic Acid
� � ��
4. Fumaric Acid � 4. Lauric Acid 9. Oleic Acid
�
� 5. Butyric Acid � 5. Linolenic Acid 10. Stearic Acid
6. Homoprotocatechuic Acid � �
� � 7. Gallic Acid � ��
� � ��
8. Protocatechuic Acid
�
9. Gentisic Acid ��
10. p-Hydroxybenzoic Acid ��
11. Benzoic Acid � � � �
� ��
� �� 12. Salicylic Acid Column: Alltima™ HP C18 HiLoad, 3µm, 150 x 4.6mm
(Part No. 87686)
Column: OA-2000, 6.5µm, 100 x 6.5mm (Part No. 9048) Mobile Phase: A: 0.1% TFA in Water B: 0.1% TFA in Acetonitrile
Mobile Phase: 0.01N Sulfuric Acid Gradient: Time: 0 10 20
Column Temp: 45°C %B: 80 100 100
Detector: UV at 228nm Column Temp: 52°C
Detector: ELSD

FOOD & BEVERAGE
HPLC Applications
� Triglycerides
�
Long Chain Fatty Acids Olive Oil
9459 � 9515
�
1. Capric Acid � 1. LLO
2. Lauric Acid 2. LLP
3. Myristic Acid 3. OOL
4. Palmitic Acid 4. POL
5. Stearic Acid 5. PPL
� � 6. OOO
� 7. OOP
� 8. PPO
� � �� 9. OOS
�
� � � � ��
Column: Prevail® C18, 3µm, 53 x 7mm Rocket™ Column: Alltima™ C18, 3µm, 150 x 4.6mm (Part No. 81387)
(Part No. 99279) Mobile Phase: A: Dichloromethane B: Acetonitrile
Mobile Phase: Acetonitrile:Methanol (75:25) Gradient: Time: 0 10 18 20
Flow Rate: 3.5mL/min %B: 70 55 70 70
Detector: ELSD Flow Rate: 1.5mL/min
Detector: ELSD
� Sesame Seed Oil Walnut Oil

9517 9522
�
� 1. LLL 1. Trilaurin (LaLaLa)
2. LLO 2. Trilinolein (LLL)
3. LLP 3. 1,2-Dilinoleyl-3-Oleyl-rac
4. OOL glycerol (LLO)
� 5. POL 4. 1,2-Dilinoleyl-3-Palmitin-
6. PPL � rac-glycerol (LLP)
��
7. OOO � 5. 1-Palmitin-2-Olein-3-
� 8. OOP Linolein rac-glycerol (POL)
�
9. PPO 6. 1,2-Dipalmytyl-3-Linolein-
� � �� 10. OOS
� ��
rac glycerol (PPL)
� � � � � ��
Column: Alltima™ C18, 3µm, 150 x 4.6mm (Part No. 81387) Column: Alltima™ C18, 3µm, 150 x 4.6mm (Part No. 81387)
Mobile Phase: A:Dichloromethane B:Acetonitrile Mobile Phase: A: Dichloromethane B: Acetonitrile
Gradient: Time: 0 10 18 20 Gradient: Time: 0 10 18 20
%B: 70 55 70 70 %B: 70 55 70 70
Additives
Evening Primrose Oil Oxygen Heterocycles in Confectionary Products
1. LGG 1. DHHM
10047 7031
2. LGL 2. Furaneol
� 3. LLL � � 3. Cyclotene
4. OGL 4. HMF
�
5. PGL � 5. HMP
6. OLL � 6. Maltol
7. OGO + PLL
� Reference: Dr. A. Lea and
8. OOO
� Dr. G. Ford, Reading Scientific
�� Services Ltd., Reading, UK.
� ��
� ��
Column: Alltima™ C18, 5µm, 250 x 4.6mm (Part No. 88056) Column: Hypercarb®, 7µm, 100 x 4.6mm (Part No. 59864750)
Mobile Phase: A: Acetonitrile B: Dichloromethane Mobile Phase: A: Perchloric Acid pH1.0
Gradient: Time: 0 40 B: Acetonitrile
%B: 30 50 Gradient: Time: 0 10 15
Flow Rate: 1.0mL/min % B: 1 35 60
Detector: ELSD Flow Rate: 1.0mL/min

LIFE SCIENCE
HPLC Applications
Proteins & Peptides/Reversed Phase

�
Protein Mix Enzymes
�
8503 9068
� �
� 1. Ribonuclease A 1. α-Chymotrypsinogen
2. Cytochrome C 2. Carbonic Anhydrase
� 3. Lysozyme Impurity
4. Lysozyme
� � 5. Myoglobin Impurity
6. Myoglobin
� � ��
Column: ProSphere C4, 300Å, 5µm, 150 x 4.6mm
™
™
(Part No. 33989) (Part No. 33989)

Mobile Phase: A: 0.15% TFA Mobile Phase: A: 0.15% TFA in Water
B: 0.13% TFA in Acetonitrile:Water (95:5) B: 0.13% TFA in Acetonitrile
Gradient: Time 0 15 Gradient: Time 0 15
%B 30 60 %B 40 55
Albumins Protein Mix

10344 10364
1. BSA 1. α-Lactalbumin
�
2. Ovalbumin 2. α-Chymotrypsinogen
�
3. Carbonic Anhydrase
4. β-Lactoglobulin
�
�
��
� � � � � ��
Column: ProSphere™ P-HR, 4µm, 150 x 4.6mm Column: ProSphere™ P-HR, 4µm, 100 x 4.6mm (Custom)
(Part No. 35082) Mobile Phase: A: 0.1% TFA in Water B: 0.08% TFA in Acetonitrile
Mobile Phase: A: 0.08% TFA in Water Gradient: Time: 0 15
B: 0.065% TFA in Acetonitrile:Water (95:5) %B: 30 55
Gradient: Time 0 20 Flow Rate: 1.0mL/min
%B 35 85 Detector: UV at 220nm
Insulins Bradykinins
9082 � 9062
3
1 1. Bovine Insulin 1. des-Pro2-bradykinin
2. Bovine Insulin Impurity 2. Bradykinin
3. Porcine Insulin �
4. Porcine Insulin Impurity
2 4
0 2 4 6 8 10 12 Min. � � � � � ��
™
™
(Part No. 32969) (Part No. 32969 )

Mobile Phase: A: 0.13% TFA in Water Mobile Phase: A: 0.15% TFA in Water
B: 0.10% TFA in Acetonitrile B: 0.13% TFA in Acetonitrile
%B: 28 38 %B: 15 25

LIFE SCIENCE
HPLC Applications
2
Bioactive Peptides Peptides
8502 3 4 9066
5
� 1. Methionine Enkephalin 1. GLY-TYR
2. Bradykinin 1 2. VAL-TYR-VAL
� � 3. Leucine Enkephalin 3. Methionine Enkephalin
� �
4. Physalaemin 4. Leucine Enkephalin
5. Substance P 5. Angiotensin II
� � � � � �� 0 2 4 6 8 10 12 14 Min.
Column: ProSphere™ C18, 300Å, 5µm, 150 x 4.6mm Column: ProSphere™ C18, 300Å, 5µm, 150 x 4.6mm
(Part No. 32969) (Part No. 32969)
Mobile Phase: A: 0.1% TFA in Water Mobile Phase: A: 0.15% TFA in Water
B: 0.085% TFA in Acetonitrile:Water (95:5) B: 0.13% TFA in Acetonitrile
Gradient: Time 0 20 Gradient: Time: 0 15
%B 20 50 %B: 10 30
Peptide Mix Peptide (P2693) Mix

10199 10342
1. Arg8-vasopressin 3. Oxytocin
� �
2. Bradykinin 4. Met-Enkephalin
�
1. Oxytocin 5. Bradykinin
� 6. Luteinizing
2. Angiotensin II
3. Angiotensin I releasing hormone
4. Eledosin � � 7. Leu-enkephalin
� �� 8. Bombesin
� � �
9. Substance P
� � � � � ��
Column: ProSphere™ C18, 100Å, 5µm, 250 x 4.6mm Column: ProSphere™ P-HR, 4µm, 150 x 4.6mm
(Part No. 35087) (Part No. 35082)
Mobile Phase: A: 0.1% TFA in Water Mobile Phase: A: 0.08% TFA in Water B: 0.065% TFA in
B: 0.1% TFA in Acetonitrile:Water (90:10) Acetonitrile:Water (95:5)
Gradient: Time 0 20 Gradient: Time 0 1 30
%B 20 50 %B 14 14 28
β-Lactoglobulin A Tryptic Digest Monoclonal Antibody Tryptic Digest

9092 10213
0 5 10 15 20 25 30 35 40 45 Min. � ��

™
Column: ProSphere C18 100Å, 5µm, 250 x 4.6mm
™
(Part No. 32969) (Part No. 35087)

Mobile Phase: A: 0.15% TFA in Water, Mobile Phase: A: 0.1% TFA in Water B: 0.08% TFA in Acetonitrile
B: 0.13% TFA in Acetonitrile Gradient: Time 0 160
Gradient: Time: 0 45 %B 0 45
%B: 5 30 Flow Rate: 0.5mL/min

LIFE SCIENCE
HPLC Applications
Proteins & Peptides/Reversed Phase

Peptide Mix on 300Å Hydrophobic Amyloid Peptides
10363 TFA Modifier at 25°C 10175
1. Methionine Enkephalin A - 0.1% TFA in Water

2. Leucine Enkephalin B - 0.085% TFA in 80% Acetonitrile
� 3. Angiotensin II Gradient 33-45% B in 30min
� 4. Neurotensin
�� 5. Angiotensin I
6. Substance P
�
Aβ(1-38) Aβ(1-40)
Aβ(1-42/43)
� � � � � �� 0 5 10 15 20 25 Min.
Column: ProSphere™ C18, 300Å, 5µm, 150 x 4.6mm Column: ZORBAX® 300 Extend-C18, 3.5µm, 150 x 2.1mm
(Part No. 32969) (Part No. 763750-902)
Mobile Phase: A: 0.1% TFA in Water Flow Rate: 0.25mL/min
B: 0.08% TFA in Acetonitrile Detector: UV at 210nm
Gradient: Time: 0 20
%B: 20 40

10369 TFA Modifier at 80°C 10176
� �
� � 1. Methionine Enkephalin
�
2. Leucine Enkephalin
3. Angiotensin II Aβ(1-42)
4. Neurotensin Aβ(1-43)
A - 0.1% TFA in Water
� 5. Angiotensin I
Aβ(1-40) B - 0.085% TFA in 80% Acetonitrile
6. Substance P
Aβ(1-38) Gradient 29-41% B in 30min
� � � � � �� 0 5 10 15 20 25 Min.
Column: Alltima HP C18, 190Å, 5µm, 250 x 4.6mm (Part No. 87679) Column:
™
ZORBAX 300 Extend-C18, 3.5µm, 150 x 2.1mm
®
Mobile Phase: A: 0.1% TFA in Water B: 0.08% TFA in Acetonitrile (Part No. 763750-902)
Gradient: Time 0 20 Flow Rate: 0.25mL/min
%B 20 40 Detector: UV at 210nm

10370 NH4OH Modifier at 25°C 10177
1. Methionine Enkephalin A - 20mM NH4OH in Water

� 2. Leucine Enkephalin B - 20mM NH4OH in 80% Acetonitrile
� � � 3. Angiotensin II Gradient 26-38% B in 30min
�
4. Neurotensin Aβ(1-42)
5. Angiotensin I Aβ(1-43)
� 6. Substance P Aβ(1-40)
Aβ(1-38)
� � � � � �� 0 5 10 15 20 25 Min.
™
Column: ZORBAX® 300 Extend-C18, 3.5µm, 150 x 2.1mm
(Part No. 35091) (Part No. 763750-902)
Mobile Phase: A: 0.1% TFA in Water B: 0.08% TFA in Acetonitrile Flow Rate: 0.25mL/min
Gradient: Time 0 20 Detector: UV at 210nm
%B 20 40

LIFE SCIENCE
HPLC Applications
Proteins & Peptides/Ion Exchange

� � Protein on a Weak Proteins on a Weak
�
Anion Exchanger 10378 Cation Exchanger � 10377
� 1. Myoglobin � 1. Myoglobin
� �
2. Conalbumin 2. Ribonuclease A
3. Bovine Serum Albumin 3. α-Chymotrypsinogen
4. β-lactoglobin A � 4. Cytochrome C
5. Lysozyme
� � � � � ��
Column: ProSphere P-WAX, 10µm, 75 x 7.5mm
™
Column: ProSphere P-WCX, 10µm, 75 x 7.5mm (Part No. P20351)
™
(Part No. P20341) Mobile Phase: A: 0.02M Potassium Phosphate pH7

Mobile Phase: A: 0.02M Tris pH8 B: 0.02M Tris, 1M Sodium Acetate pH8 B: 0.02M Potassium Phosphate, 0.5M Sodium Chloride pH7
Gradient: Time 0 1 20 25 Gradient: Time 0 30 35
%B 0 28 70 0 %B 0 75 0
Flow Rate: 1mL/min Flow Rate: 1.0mL/min
Proteins on a Strong Proteins on a Strong

�
Anion Exchanger Cation Exchanger
10381 � 10376
�
� �
1. Myoglobin 1. Myoglobin
� �
� �
3. Bovine Serum Albumin 3. α-Chymotrypsinogen
4. β-lactoglobin A 4. Cytochrome C
5. Lysozyme
� � � � � ��
Column: ProSphere™ P-SAX, 10µm, 75 x 7.5mm Column: ProSphere™ P-SCX, 10µm, 75 x 7.5mm (Part No. P20347)
(Part No. P20336) Mobile Phase: A: 0.02M Potassium Phosphate pH 7
Mobile Phase: A: 0.02M Tris pH8 B: 0.02M Potassium Phosphate,
B: 0.02M Tris, 1.0M Sodium Acetate pH8 0.5M Sodium Chloride pH 7
Gradient: Time 0 1 20 25 Gradient: Time 0 30 35
%B 0 28 30 0 %B 0 75 0
Strong Anion Exchange of Egg White High Speed Human Hemoglobin Analysis
10401 10397
� � � � � ��
Column: ProSphere P-SAX-HR, 7µm, 50 x 4.6mm
™
(Part No. P20334) � � � � ��

Mobile Phase: A: 0.02M Tris-HCl pH 8.0 Column: ProSphere™ P-SCX-NP, 2.5µm, 35 x 4.6mm (Part No. P20346)
B: 0.02M Tris-HCl,1M NaCl pH 8.0 Mobile Phase: A: 0.05M NaCl, 0.02M bis-Tris-HCl pH 6.0
Gradient: Time 0 15 B: 0.2M NaCl, 0.02M bis-Tris-HCl pH 6.0
%B 0 100 Gradient: Time 0 3
Flow Rate: 1.0mL/min %B 0 100
Column Temp: 25°C Detector: Vis at 415nm

LIFE SCIENCE
HPLC Applications
Proteins & Peptides/Size Exclusion Proteins/Hydrophobic Interaction

Proteins (<60,000 DA) by SEC 1 Proteins by HIC
10371 10388
1. Ferritin 1. Myoglobin
5
� 3. Ovalbumin 3. Lysozyme
�
4. Carbonic anhydrase 4. α-Chymotrypsin
3
� � 5. Myoglobin 5. α-Chymotrypsinogen
� � � 6. Cytochrome C 2
7. GLY-TYR 4
� � � � � �� 0 15 30 45 Min.
Column: ProSphere™ SEC 125, 5µm, 300 x 7.8mm Column: ProSphere™ P-HIC Ether, 10µm, 75 x 7.5mm
(Part No. P20325) (Part No. P20316)
Mobile Phase: 0.3M Sodium Chloride, Mobile Phase: A: 1.8M Ammonium Sulfate, 0.1M Potassium Phosphate
0.05M Potassium Phosphate pH 7.0 pH 7.0 B: 0.1M Potassium Phosphate pH 7.0
Flow Rate: 1.0mL/min Gradient: Time 0 60
Detector: UV at 254nm %B 0 100
Proteins (<120,000 DA) by SEC �
Proteins by HIC
10372 � 10389
�
� 1. Thyroglobulin � 1. Myoglobin
2. IgG 2. Ribonuclease A
3. Phosphorylase b � 3. Lysozyme
4. Ovalbumin � 4. α-Chymotrypsin
�
� � �
5. Ribonuclease A 5. α-Chymotrypsinogen
6. GLY-TYR
� � � � � ��
Column: ProSphere SEC 250, 5µm, 300 x 7.8mm
™
Column: ProSphere P-HIC Phenyl, 10µm, 75 x 7.5mm
™
(Part No. P20326) (Part No. P20320)

Mobile Phase: 0.3M Sodium Chloride, Mobile Phase: A: 1.8M Ammonium Sulfate,
0.05M Potassium Phosphate pH 7.0 0.1M Potassium Phosphate pH 7.0
Flow Rate: 1.0mL/min B: 0.1M Potassium Phosphate pH 7.0
Detector: UV at 254nm Gradient: Time 0 60
%B 0 100
Purified Human Immunoglobulin M on Size Exclusion Proteins by HIC

3 5
10400 10390
1. Myoglobin
1
2. Ribonuclease A
2 4 3. Lysozyme
4. a-Chymotrypsin
5. a-Chymotrypsinogen
0 2 4 6 8 10 Min.
Column: ProSphere P-HIC NP-Butyl, 2.5µm, 33 x 4.6mm
™
(Part No. P20314)

� � �� Mobile Phase: A: 2.3M Ammonium Sulfate,
0.1M Potassium Phosphate pH 7.0
Column: ProSphere SEC 250, 5µm, 300 x 7.8mm
™
B: 0.1M Potassium Phosphate pH 7.0
(Part No. P20326) Gradient: Time 0 12
Mobile Phase: 0.2M NaCl, 0.1M Phosphate pH 7.0 %B 0 100

LIFE SCIENCE
HPLC Applications
Peptides/Ion Exchange Proteins /Affinity

Angiotensins by Cation Exchange Affinity purification of trypsin
�� 10396 10392
��
��
��
��
1. Unretained Impurities
2. Purified Trypsin
��
��
� � ��
Column: ProSphere™ P-SCX-HR, 7µm, 50 x 4.6mm � ��
(Part No. P20344) Column: ProSphere™ ABA, 10µm, 75 x 7.5mm
Mobile Phase: A: 0.02M Sodium Acetate pH 5.0 (Part No. P20353)
B: 1M NaCl, 0.02M Sodium Acetate pH 5.0 Mobile Phase: A: 0.5M NaCl, 2mM CaCl2, 0.05M Tris-HCl pH 8.0
Gradient: Time: 0 20 B: 0.5M NaCl, 2mM CaCl2, 0.05M Glycine-HCl pH 2.8
%B 0 100 Gradient: Time 0 10 10.1 30
Flow Rate: 1.0mL/min %B 0 0 100 100
Amino Acids
Amino Acids without Derivatization Thyroid Chemicals
1. Gly 5. Ala/Thr 9. Pro 1. DL-Tyrosine
9413 8282
5 2. Ser/Asn 6. Glu 10. Arg 2. 3-Iodo-Tyrosine
4 3. Asp 7. Cys/Lys 11. Val 3. 3,5- Diiodo-D-Tyrosine
3 4. Gln 8. His 12. Met 4. DL-Thyroxine
11 13. Tyr 2 5. 3,3,5-Triiodo-L-Thyronine
2 7 13 14. Ile 1 3
1 12 15. Leu
14 5
16 16. Phe 4
17 17. Trp
6 15
8 9
10
0 5 10 15 20 25 Min. 0 2 4 6 8 10 12 Min.
Column: Prevail™ C18, 5µm, 250 x 4.6mm (Part No. 99210) Column: Platinum™ EPS C18, 5µm, 150 x 4.6mm
Mobile Phase: A: 5mM Heptafluorobutyric Acid, 0.7% TFA (Part No. 32214)
B: Acetonitrile Mobile Phase: 0.05M KH2PO4, pH 3.2:Acetonitrile (65:35)
Gradient: Time: 0 6 8 25 Flow Rate: 1.0mL/min
%B: 0 0 15 35 Detector: UV at 254nm
Detector: ELSD
Catechols
Phenylalanine Catecholamines
8770 10148
1. VMA
2. 5-HIAA
O 3. HVA
�
OH �
NH2 �
4 6 8 10 12 Min. � ��
Column: Chirobiotic T , 250 x 4.6mm (Part No. 12053)
™
Column: Inertsil CN-3, 5µm, 150 x 4.6mm
®
Mobile Phase: Ethanol:Water (80:20) (Part No. GL-01940)

Flow Rate: 1.0mL/min Mobile Phase: 0.05M NaH2PO4 pH 3.0:Acetonitrile (99:1)
Column Temp: 30°C
Detector: ECD, 700 mV vs. Ag/AgCl

LIFE SCIENCE
HPLC Applications
Nucleic Acids
Nucleosides Nucleosides
� 10347 8245
� � 1. Cytidine 1. Cytidine
2. Uridine 2. Uridine �
3. Xanthosine 3. Xanthosine �
� �
4. Guanosine 4. Guanosine �
5. Adenosine 5. Adenosine �
� � � � � ��
Column: Alltima HP EPS C18, 5µm, 150 x 4.6mm
™
Column: Platinum EPS C18, 5µm, 250 x 4.6mm
™
(Part No. 87715) (Part No. 32246)

Mobile Phase: A: 0.03M Potassium Phosphate pH3.2 Mobile Phase: A: 0.03M KH2PO4 pH3.2 B: Acetonitrile
B: Acetonitrile Gradient: Time: 0 2 20
Gradient: Time 0 2 20 %B 5 5 30
%B 5 5 30 Flow Rate: 0.7mL/min
Nucleosides Nucleic Acid Bases

9410 � 9411
� 1. Cytidine 1. Cytosine
2. Uridine 2. Uracil
� 3. Xanthosine 3. Guanine
� � �
4. Guanosine � 4. Adenine
5. Adenosine � 5. Thymine
�
�
� � � � ��
®
™
Mobile Phase: 0.025M KH2PO4 pH 3.0:Acetonitrile:(96:4) Mobile Phase: 0.025M KH2PO4 pH3.0:Acetonitrile:(98:2)
Flow Rate: 1mL/min Flow Rate: 1.0mL/min
Detector: UV at 254 Detector: UV at 254
Nucleosides by Affinity Fast High Resolution Separation of DNA Fragment Digest

� 1. Cytidine 1. 51 base pairs 10. 213 base pairs
� 10393 10395
2. Uridine 2. 57 base pairs 11. 234 base pairs
3. Guanosine 3. 64 base pairs 12. 192 base pairs
4. Adenosine 4. 80 base pairs 13. 267 base pairs
5. 89 base pairs 14. 434 base pairs
� 6. 104 base pairs 15. 540 base pairs
��
��
��

� 8. 124 base pairs 17. 458 base pairs
��
��
��
��
��
��
��
��
��
��
��
��
��
� ��
Column: ProSphere™ Boronate, 10µm, 75 x 7.5mm Column: ProSphere™ P-WAX-NP, 2.5µm, 35 x 4.6mm (Part No. P20339)
(Part No. P20354) Mobile Phase: A: 0.02M Tris-HCl pH 9.0
Mobile Phase: 0.1M Phosphate, pH 8.0 B: 1M NaCl, 0.02M Tris-HCl pH 9.0
Flow Rate: 1.0mL/min Gradient: Time 0 15
Detector: UV at 280nm %B 48 65

LIFE SCIENCE
HPLC Applications
Miscellaneous
Total E.coli RNA by Anion Exchange Phospholipids
10402 1. 1,2-Phosphatidyl-ethanolamine 10314
� 2. Phosphatidyl-DL-glycerol
�� 3. Phosphatidylinositol
��
4. 1,2-Phosphatidyl-L-serine
��
5. Phosphatidylcholine
�� 6. Sphingomyelin
� �
��
��
� ��
Column: ProSphere™ P-WAX, 10µm, 75 x 7.5mm (2) Column: Prevail™ Silica, 3µm, 150 x 4.6mm (Part No. 99275)
(Part No. P20341) Mobile Phase: A: Isopropanol B: Hexane C: Water
Mobile Phase: A: 0.3M NaCl, 0.1M Tris-HCl pH 7.6 Gradient: Time 0 7 15
B: 1.0M NaCl, 0.1M Tris-HCl pH 7.6 %B 40 40 40
Gradient: Time 0 90 %C 2 8 8
%B 0 30 Flow Rate: 1.0mL/min
Benzyl Acids 3 Aromatic Acids

1
9538 7373
2 3
1. 3-Amino-4-hydroxybenzoic Acid 1. DL-4-Hydroxy-3-methoxy-mandelic Acid
2. 4-Hydroxybenzoic Acid 2. Homovanillic Acid
3. Benzoic Acid 3. Vanillic Acid
4. 4-Phenylbutyric Acid 4. Salicylic Acid
1 5. 5-Phenylbutyric Acid 5. Benzoic Acid
2
4 5
4 5
0 2 4 6 8 10 12 14 Min. 0 1 2 3 4 5 6 7 8 9 10 11 Min.
™
(Part No. 88640) Mobile Phase: 0.05M KH2PO4 pH 3.2:Methanol (60:40)
Mobile Phase: 0.025M KH2PO4 pH2.5:Acetonitrile (60:40) Flow Rate: 1.0mL/min
Monoaromatics Alcohols
2
7 8982 � 5440
9 10
1. Uracil � 1. Glycerol
2. Benzamide � 2. Methanol
3. Benzyl Alcohol � 3. Ethanol
3 � ��
4 4. Phenol � 4. iso-Propanol
5. Methylbenzoate � � � 5. tert-Butanol
6. Acetophenone �� 6. Propanol
6 7. N,N-diethyl-m-toluamide 7. sec-Butanol
5 ��
8. Toluene 8. iso-Butanol
1 9. Benzophenone 9. tert-Pentanol
8
10. Biphenyl 10. Butanol
11. iso-Pentanol
12. Pentanol
0 1 2 3 4 Min. � � � ��
Column: Platinum™ EPS C18, 3µm, 53 x 7mm Rocket™ Column: OA-1000, 9µm, 300 x 6.5mm (Part No. 9046)
(Part No. 50573) Mobile Phase: 0.01N Sulfuric Acid
Mobile Phase: 0.05M KH2PO4 pH 3:Methanol (50:50) Flow Rate: 0.8mL/min
Detector: UV at 254nm Detector: RI

E N V I R O N M E N TA L
HPLC Applications
Pesticides
Organophosphates Organochlorines
10346 8437
�
1. Methyl parathion 1. Methoxychlor
2. Dichlorvos 2. Dieldrin
1 3 3. Ethoprophos 3. Heptachlor
4. Azinphos 4. 4,4-DDT
�
2 4
�
�
0 4 8 12 16 20 24 Min. � � � � ��
Column: Alltima HP EPS C18, 5µm, 150 x 4.6mm
™
Column: Platinum C18, 5µm, 150 x 4.6mm (Part No. 32043)
™
(Part No. 87715) Mobile Phase: 0.025M K2HPO4 pH7:Methanol (25:75)

Mobile Phase: Water:Methanol (60:40) Flow Rate: 1.0mL/min
Triazine Herbicides Herbicides

8671 10355
2
1. Simazine 3 1. Fenuron
2. Simetryn 2. Monuron
� 3. Prometon 3. Diuron
4
4. Atrazine 4. Linuron
� 5. Ametryn
�
6. Prometryn
� � 7. Terbutryn
�
� 1
� � � � � �� 0 1 2 3 4 5 6 7 8 Min.
Column: Platinum™ EPS C18, 1.5µm, 33 x 7mm Rocket™ Column: Alltima HP CN, 5µm, 150 x 4.6mm
™
(Part No. 50575) (Part No. 87769)

Mobile Phase: 0.025M KH2PO4 pH3:Acetonitrile (65:35) Mobile Phase: Water:Acetonitrile (60:40)
� � Herbicides & Fungicides Bipyridylium Herbicides

8093 1 8439
2
� 1. Asulam 1. Diquat
2. MCPP 2. Paraquat
3. Oxine-copper
4. Thiuram
5. Iprodione
6. Bensulide
7. Pencycuron
� �
�
�
0 2 4 6 8 Min.
� � � � � ��
Column: Platinum™ Silica 100Å 3µm, 30mm x 4.6mm (Custom)
Column: Inertsil® ODS-3, 5µm, 150 x 4.6mm Mobile Phase: 0.01M Tetramethylammonium Hydroxide and
(Part No. GL-01731) 0.15M Ammonium Sulfate in Water
Mobile Phase: 0.05M KH2PO4 pH3:Acetonitrile (40:60) pH 3.0 with 5M Sulfuric Acid
Column Temp: 40°C Detector: UV at 310nm until 3 minutes
Detector: UV at 230nm UV at 260nm after 3 minutes

HPLC Applications
PAHs
Polyaromatic Hydrocarbons
9400
1. Benzene 10. Benz[a]anthracene

46 11 2. Naphthalene 11. Chrysene
7 3. Acenaphthylene 12. Benzo[b]fluoranthene
12
5 14 4. Fluorene 13. Benzo[k]fluoranthene
16 5. Acenaphthene 14. Benzo[a]pyrene
13 6. Phenanthrene 15. Dibenz[a,h]anthracene
7. Anthracene 16. Benzo[g,h,i]perylene
8
8. Fluoranthene 17. Indeno[1,2,3,c,d]pyrene
9 9. Pyrene
1 3 17 Column: Prevail™ C18, 5µm, 150 x 4.6mm (Part No. 99208)

15
2 Mobile Phase: A: Water B: Acetonitrile
10
Gradient: Time: 0 4 15
%B: 72 72 100
0 2 4 6 8 10 12 14 16 18 20 Min. Flow Rate: 1.5mL/min
Detector: UV at 254
Polynuclear Aromatic Hydrocarbons

�� 10147
1. Naphthalene 10. Chrysene

2. Acenaphthylene 11. Benzo[b]fluoranthene
3. Acenaphthene 12. Benzo[k]fluoranthene
4. Fluorene 13. Benzo[a]pyrene
5. Phenanthrene 14. Dibenz[a,h]anthracene
�� 6. Anthracene 15. Benzo[g,h,i]perylene
��
7. Fluoranthene 16. Indeno[1,2,3,c,d]pyrene
8. Pyrene
�� 9. Benz[a]anthracene
��
� �� Column: Inertsil® ODS-P, 5µm, 250 x 4.6mm
�� (Part No. GL-02002)
��
Mobile Phase: A: Water:Methanol (30:70) B: Methanol
��
� � � Gradient: Time: 0 5 20
%B: 0 0 100
Column Temp: 40°C
� �� Detector: UV at 254
PAH (EPA 610)

7707
6
1. Naphthalene 9. Benz[a]anthracene
2. Acenaphthylene 10. Chrysene
3. Acenaphthene 11. Benzo[b]fluoranthene
2 11
4. Fluorene 12. Benzo[k]fluoranthene
5 5. Phenanthrene 13. Benzo[a]pyrene
6. Anthracene 14. Dibenz[a,h]anthracene
10
7. Fluoranthene 15. Benzo[g,h,i]perylene
4 8. Pyrene 16. Indeno[1,2,3,c,d]pyrene
1 7 9 Column: Hypersil® Green PAH, 5µm, 100 x 4.6mm

13 (Part No. 59864511)
12 15 16 Mobile Phase: A: Water:Acetonitrile (99:1)
3 B: Water:Acetonitrile(1:99)
8 14
Gradient: Time: 0 5 25
%B: 50 50 100
0 5 10 15 20 25 Min.

HPLC Applications
Water Pollutants 1. 4-Amino-5-hydroxy-2,7-naphthalene-disulfonic Acid

2. Hydroquinone
Semipolar & Highly Hydrophilic 3. Benzylamine
Wastewater Contaminants 4. 3'-Aminoacetophenone
� 5. Phenol
8447
�� 6. 4-Hydroxyphenylacetic Acid
� � �� 7. 1-Naphthalene Sulfonic Acid
��
� � �� 8. 2,4-Dihydroxybenzoic Acid
��
� �� 9. 1-Naphthalene Sulfonic Acid Degradant
10. 4-Hydroxy-3-methoxybenzoic Acid
11. Phenylacetic Acid
� ��
12. m-Cresol
Column: Platinum EPS C18, 5µm, 250 x 4.6mm (Part No. 32246)
™ 13. 4-Hydroxycinnamic Acid
Mobile Phase: A: 0.03M NaH2PO4 pH2.5 14. trans-Cinnamaldehyde
B: Acetonitrile:Methanol:Water (40:50:10) 15. trans-Cinnamaldehyde Degradant
Gradient: Time: 0 3 13 20 25 30 40 45 55 16. trans-Cinnamaldehyde Degradant
%B: 5 5 10 10 20 40 75 80 80 17. 2-Naphthalenethiol Degradant
Flow Rate: 1.0mL/min 18. 2-Naphthalenethiol
Detector: UV at 210nm 19. 2-Naphthalenethiol Degradant
Phthalates Phenols
Phthalic Acid Dialkyl Esters Endocrine Disruptors
10149 10150
1. diethylester 1. Phenol
2. di-isopropylester 2. Diethylphthalate
3. di-n-butylester 3. Bisphenol-A
2 4
4. di-isobutylester 4. 2,4-Dichlorophenol
� 5. dicyclohexylester 5. 4-Octylphenol
6. di-n-heptylester 6. 4-Nonylphenol
5
�� 7. di-2-ethylhexylester
� 1
8. di-n-decylester 3
9. Phthalic Acid di-n-dodecylester
�� 6
� �
� �� 0 2 4 6 8 10 12 14
Column: Inertsil® C8-3, 5µm, 150 x 4.6mm Column: Inertsil® CN-3, 5µm, 250 x 4.6mm
(Part No. GL-01900) (Part No. GL-01941)
Mobile Phase: A: Water B: Acetonitrile Mobile Phase: 0.2M KH2PO4 pH 3.0:Acetonitrile (55:45)
Gradient: Time: 0 5 10 Flow Rate: 1.0mL/min
%B: 75 75 100 Column Temp: 40°C
Column Temp: 40°C
Detector: UV at 254
Nitroaromatics Explosives
Nitroaromatics (Normal phase) Nitroaromatic and Nitroamine Explosives
3 1. Nitrobenzene 10358 8279
1. HMX 7. TNT
2. 2,6-Dinitrotoluene 2. RDX 8. 4-A-DNT
� �
3. 1,3-Dinitrobenzene 3. TNB 9. 2,6DNT
4. 4-Nitrophenol 4. DNB 10. 2,4DNT
2 5. Tetryl 11. 2-NT
6. NB 12. 3-NT
� 13 4-NT
� � � ��
�
4 ��
� �
1
0 1 2 3 4 5 6 7 8 Min.
� � � � � ��
Column: Alltima HP CN, 5µm, 150 x 4.6mm ™
Column: Platinum™ C18, 5µm, 250 x 4.6mm (Part No. 32064)
(Part No. 87769) Mobile Phase: Water:Methanol (50:50)
Mobile Phase: Hexane:Isopropanol (85:15) Flow Rate: 0.7mL/min

E N V I R O N M E N TA L /
MISCELLANEOUS HPLC Applications
Aflatoxins
Aflatoxins Aflatoxins
10144 10437
� �
� 1. Aflatoxin G2 1. Aflatoxin B1
2. Aflatoxin G1 2. Aflatoxin G1
3. Aflatoxin B2 3. Aflatoxin B2
4. Aflatoxin B1 � 4. Aflatoxin G2
� � �
� � ��
� � � � � ��
Column: Inertsil® ODS-3V, 5µm, 150 x 4.6mm Column: Alltima™ HP Silica, 5µm, 150 x 4.6mm
(Part No. GL-01801) (Part No. 87795)
Mobile Phase: Water:Methanol (50:50) Mobile Phase: Methylene Chloride:Methanol:Water (99.35:0.5:0.15)
Column Temp: 40°C Detector: ELSD
Detector: UV at 365
Paraffins Fire Retardants

Paraffin Analysis Brominated Flame Retardants
10429 2 10261
1
Paraffin C20-C30 1. Hexabromo
cyclododecane
2. Tetrabromo
bisphenol A (allyl ether)
� � � � � ��
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Min.
Column: Alltima HP C18 HiLoad, 3µm, 100 x 4.6mm
™
Column: Alltima C18, 5µm, 250 x 4.6mm
™
(Part No. 87685) (Part No. 88056)

Mobile Phase: Acetonitrile:Dichloromethane (60:40) Mobile Phase: Water:Acetonitrile (5:95)
Cooking Oil Bucky Balls

� Used Cooking Oil “Bucky Balls”
7215 7210
�
1. Polymerized 1. C70
2. Polymerized 2. C60
�
3. Triglycerides
4. MW 1800-3000
5. Triglycerides MW 930
6. Diglycerides MW 620
7. Monoglycerides MW 400
�
� 8. Free Fatty Acids MW 300
�
�
�
� �
� ��

Column: Jordi DVB, 10 Å, 500Å, 500mm x 10mm (2)
3
Column: Jordi DVB, 500Å, 500 x 10mm (6)
(Part No.s 100571, 100567) (Part No. 100567)
Mobile Phase: THF Mobile Phase: Trichlorobenzene
Detector: UV at 254nm Detector: RI

MISCELLANEOUS
HPLC Applications
Polymers
Polyurethane Curatives 7472 Polystyrene 7213
� 1. 8.42M
2. 1.3M
� 3. 791K
1. Polytetramethylene Glycol 4. 354K
2. Ethecure 300 � 5. 98.9K
� 6. 43K
� � ��
� � � 7. 10.1K
�
� 8. 2.6K
9. 530K
10. 120K
��
Column: Jordi DVB, 500Å, 500mm x 10mm (6) Column: Jordi DVB, Mixed Bed Linear, 500mm x 10mm
(Part No. 100567) (Part No. 100583)
Mobile Phase: Chloroform Mobile Phase: THF
Detector: RI Detector: RI
Polyethylene and Hydrocarbon Standards

7220
��
��
1. PE-32000 MW 12. C40 23. C18
�� 2. PE-2155 MW 13. C38 24. C16
�� 3. C58 14. C36 25. C14
�� 4. C56 15. C34 26. C12
5. C54 16. C32 27. C10
��
6. C52 17. C30 28. C9
�� 18.
7. C50 C28 29. C8
�
�� 8. C48 19. C26 30. C7
��
9. C46 20. C24 31. C6
� �� 10. C44 21. C22 32. C5
��
�� 11. C42 22. C20
� ��
� ��
� Column: Jordi DVB, 500Å, 500mm x 10mm (6)
� �� (Part No. 100567)
�� Mobile Phase: Trichlorobenzene
� �� Flow Rate: 1.5mL/min
��
Column Temp: 145°C
Detector: RI
� ��
Tween 80 Dimethicone
7809 8636
� � ��
™
Column: Alltima™ C18, 5µm, 250 x 4.6mm Mobile Phase: A: Acetonitrile B: Chloroform
(Part No. 88056) Gradient: Time: 0 20
Mobile Phase: Water:Methanol (40:60) %B: 25 75

MISCELLANEOUS
HPLC Applications
Phenones/Fast Gradient Column Formats

Phenone Homologs Phenone Homologs
9462 9467
� � �
�
1. Uracil � � � 1. Uracil
2. Theophylline 2. Theophylline
� � 3. Acetophenone 3. Acetophenone
� 4. Propiophenone � 4. Propiophenone
� 5. Butyrophenone 5. Butyrophenone
� �
6. Benzophenone � 6. Benzophenone
7. Valerophenone � � 7. Valerophenone
� 8. Heptanophenone 8. Heptanophenone
9. Octanophenone 9. Octanophenone
� � � ��
Column: Alltima C18, 3µm, 20 x 2.1mm Expedite™ (Part No. 43803)
™
Columns: Alltima™ C18, 3µm, 20 x 4.6mm Expedite™ (Part No. 43451)
Mobile Phase: A: 0.1% TFA in Water B: 0.08% TFA in Acetonitrile Mobile Phase: A: 0.1% TFA in Water B: 0.08% TFA in Acetonitrile
%B: 5 100 %B: 5 100
Phenone Homologs Phenone Homologs

� � � 9471 � 9468
�
1. Uracil � � 1. Uracil
2. Theophylline 2. Theophylline
� 3. Acetophenone 3. Acetophenone
� 4. Propiophenone � 4. Propiophenone
�
5. Butyrophenone 5. Butyrophenone
6. Benzophenone � � 6. Benzophenone
�
� 7. Valerophenone 7. Valerophenone
� � �
8. Heptanophenone 8. Heptanophenone
9. Octanophenone 9. Octanophenone
� � � ��
Columns: Alltima C18, 3µm, 10 x 2.1mm Expedite™ (Part No. 43836) Columns: Alltima™ C18, 3µm, 10 x 4.6mm Expedite™ (Part No. 43837)
Mobile Phase: A: 0.1% TFA in Water B: 0.08% TFA in Acetonitrile Mobile Phase: A: 0.1% TFA in Water B: 0.08% TFA in Acetonitrile
%B: 5 100 %B: 5 100
Chelators
Phenone Homologs Metal Chelators
9460
� 8584
1 4 5
7
3 1. Uracil 1. 2,2’-Dipyridyl
2. Theophylline 2. 8-Hydroxyquinoline
3. Acetophenone �
6 8 4. Propiophenone
9 5. Butyrophenone
6. Benzophenone
2 7. Valerophenone
8. Heptanophenone
9. Octanophenone
0 1 2 3 Min.
Column: Prevail C18, 3µm, 20 x 2.1mm, Expedite™

™
� � � ��
(Part No. 43827)
Mobile Phase: A: 0.1% TFA in Water Column: Alltima™ C18, 5µm, 150 x 4.6mm (Part No. 88052)
B: 0.08% TFA in Acetonitrile Mobile Phase: 0.025M K2HPO4 pH7.0:THF:Methanol (50:25:25)
Gradient: Time: 0 2 3 Flow Rate: 1.0mL/min
%B: 0 100 100 Detector: UV at 254nm


HPLC Fenitoina

Hochgeladen von

Dokumentinformationen

Originalbeschreibung:

Originaltitel

Copyright

Verfügbare Formate

Dieses Dokument teilen

Dokument teilen oder einbetten

Freigabeoptionen

Stufen Sie dieses Dokument als nützlich ein?

Sind diese Inhalte unangemessen?

Copyright:

Verfügbare Formate

HPLC Fenitoina

Hochgeladen von

Copyright:

Verfügbare Formate

PHARMACEUTICALS

Basic Drugs Naproxen

This is a partial listing of our chromatograms. Visit chromatography.com for our

Tricyclic Amines Alprazolam and Triazolam by the

1. Fluphenazine HCL 1. Triazolam

Benzodiazepines and Metabolites Universal Combinatorial High Speed

1. Chlordiazepoxide degradant 5. Chlordiazepoxide 1. Theophylline

This is a partial listing of our chromatograms. Visit chromatography.com for our

� 1. Barbital 1. p-Aminobenzoic Acid

Column: Alltima™ C8, 5µm, 150 x 4.6mm (Part No. 88072)

This is a partial listing of our chromatograms. Visit chromatography.com for our

� Very Polar Bases Basic Antihistamines at High pH

Basic Compounds in Mid pH Range OTC Antihistamine and Excipients

(Part No. 883668-901) (Part No. 35101)

This is a partial listing of our chromatograms. Visit chromatography.com for our

This is a partial listing of our chromatograms. Visit chromatography.com for our

Mobile Phase: 0.025M KH2PO4, pH3.0:Acetonitrile (70:30) (Part No. ZR03-1546)

Calcium Channel Blockers Drug Mix

� Opioids Morphines & Metabolites

This is a partial listing of our chromatograms. Visit chromatography.com for our

Hydrocortisone Acetate Cream Estropipate Sample

1. Methyl Paraben 1. Estrone Sulfate

This is a partial listing of our chromatograms. Visit chromatography.com for our

8 Diuretics - High Throughput

This is a partial listing of our chromatograms. Visit chromatography.com for our

Aminoglycoside Antibiotics Antibacterials

Column: Alltima C18, 5µm, 250 x 4.6mm (Part No. 88056)

Tylosin and Monensin Sulfa Drugs

� 1. Tylosin (400ppm) 1. Sulfanilamide

This is a partial listing of our chromatograms. Visit chromatography.com for our

� ��� � ��� � ��� ������ � � � � � ������

This is a partial listing of our chromatograms. Visit chromatography.com for our

Ginseng Black Cohosh

(Part No. 35101) (Part No. 99210)

(Part No. 50605) (Part No. 81102)

This is a partial listing of our chromatograms. Visit chromatography.com for our

Flavonoid Mix Flavonoid Derivatives

St. John’s Wort Echinacea

1. Pseudohypericin 1. Protocatechuic Acid

This is a partial listing of our chromatograms. Visit chromatography.com for our

(Part No. 50577) (Part No. 50605)

Goldenseal Root Evening Primrose

This is a partial listing of our chromatograms. Visit chromatography.com for our

Fat-Soluble Vitamins and Tocopherols Vitamin E Soft Gel Tablet

Water-Soluble Vitamins Water-Soluble Vitamins

1. Thiamine 1. Ascorbic Acid

This is a partial listing of our chromatograms. Visit chromatography.com for our

1. Dextrose 5. Maltopentaose 1. Dextrose

Mono-, Di-, Tri- Saccharides 10009

�� 1. iso-Erythritol 9. Maltose 1. Fructose

This is a partial listing of our chromatograms. Visit chromatography.com for our

Sugar Alcohols Microbore Analysis of Glucopyranosides

3 Imported Ale Wine Analysis - 2000 Sipaska Rosé

Wine Analysis by Beverage Additives

1. Citric Acid � 1. Ascorbic Acid

This is a partial listing of our chromatograms. Visit chromatography.com for our

Column: Prevail Organic Acid, 5µm, 150 x 4.6mm

(Part No. 88640) (Part No. 88640)

4 1. Lauric Acid 1 1. Tartaric Acid

1. Oxalic Acid � 1. Caproic Acid 6. Myristic Acid

This is a partial listing of our chromatograms. Visit chromatography.com for our

� Sesame Seed Oil Walnut Oil

� ��

0 5 10 15 20 25 30 35 40 45 Min. � ��

(Part No. P20334) � � � � ��

� ��

��