Poster-27

ISOLATION AND İDENTIFICATION OF INFECTIOUS PANCREATIC NECROSIS

VIRUS (IPNV) FROM RAINBOW TROUT (ONCORHYNCHUS MYKISS,
WALBAUM) FINGERLINGS IN TURKEY

Ugur Degirmenci1*, Hasmet Cagirgan1, Feyza Sanver2, Buket Ozkan Ozyer1 and
Fikri Balta3
1
Ege University Facultyof Fisheries Department of Fish Diseases, 35440,Iskele-Urla
Izmir,Turkey; 2Çanakkale OnsekizMartUniversity Facultyof Fisheries Terzioglu
Kampusü 17100Canakkale, Turkey; 3 Karadeniz Technical University, Facultyof
Fisherices, 53100Rize, Turkey

13th EAFP International Conference“Diseases of Fish and Shellfish”

17th – 21th September, 2007 Grado, Italy

An outbreak was observed in a rainbow trout farm at District Aydin in March 2005 with high
mortality. The diseased rainbow tout fingerlings were showed darkening of the skin
exophtalmus and erratic swimming. Diseased live samples were sent to Faculty of Fisheries of
Ege University. Diseased fish were investigated in pathogenic parasit, bacteria and viruses.
CPE was developed 2 days after inoculation to EPC and BF cellines and lysed compleately
after 7 days. A few number of İchthyophthirus multifilis trophozoit were found on the gills
and skin. Flavobacterium psychrophilum were also isolated from kidney and spleen of
symptom showing fry on AOA . İdentification of virus was carried out by using IPNV-Ag
ELISA (Testline), VHSV-ag (Testline) and IHNV-Ag (Biox) ELISA test kits. The result
showed that isolated virus was IPNV. Isolate was serotyped using VR-299, Ab and Sp
antisera. The virus was neutralised equally with serotype Sp and Ab. In this research the first
time isolation in celline and serotyping of IPNV from Turkey were reported.

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