1.Which of the following tests is used extensively to detect microbial antigens rapidly (5 min or less)?

Inert particles are sensitized with either antigen or antibody. A. Latex agglutination (LA)* B. Enzyme-linked immunosorbent assay (ELISA) C. Enzyme multiplied immunoassay test (EMIT) D. Counterimmunoelectrophoresis (CIE) E. Coagglutination (COA) 2. Which of the following involves separation of antigens by size on a gel, followed by diffusion and precipitation? A. Flow cytometry B. Double diffusion immunoassay C. Immunoelectrophoresis* D. Direct immunosorbent assay E. Indirect immunosorbent assay 3. The material from patient for statement agglutination reaction with the purpose serodiagnostic is: A. Serum* B. diagnosticum C. culture D. agglutinated serum E. physiological solution 4. Precipitated anthrax serum contains: A. the causative agent of anthrax B. anthrax toxins C. spores of bacillus anthracis D. antibodies to a specific antigen of bacillus anthracis* E. normal antibodies 5. Complement fixation refers to A. the ingestion of C3b-coated bacteria by macrophages B. the destruction of complement in serum by heating at 56 C for 30 minutes C. the binding of complement components by antigen-antibody complexes* D. the interaction of C3b with mast cells E. the binding of complement components by bacteria 6. The Classical way of activation complement is carried out: A. without participation of antibodies B. at participation properdin C. without formation membrane attack complex D. at formation of an antigen antibody complex* E. at formation L-transformations

7. Indicator system of CFT contains: A. complement+hemolysin B. red cells and hemolysin* C. patients serum D. red blood cells of I (0) groups and hemolysin E.specific antigen 8. Immune hemolysis test is used with the purpose: A. definitions of group of blood B. titrations of complement* C. definitions of osmotic stability of RBCs D. serological identification of microorganisms E. estimations of phagocytic activity of neutrophils 9. Patients immune status examination reveals altered classic pathway of complement activation. Which Ig are responsible for the activation of the comlement? A. Ig M, Ig G* B. Ig A, Ig M C. Ig D, Ig G D. Ig E, Ig A E. Ig M, Ig D 10. At research of pair serums criterion of estimation CFT at serological diagnosis of bacterial infections: A. norm of controls B. full absence of hemolysis C. partial hemolysis D. diagnostic titer E. Increase of a titer of antibodies in 4 and more times* 11. Choose a correct answer in relation to the Grubbers reaction (unfolded agglutination test) A. It uses for determination of title of antibodies in the patients serum B. the components of this test are patients serum and diagnosticum C. it uses for the exposure of serovar of bacterium during serological authentication* D. it uses dilution of bacterial culture and specific serum E. for making this test lisozyme is needed 12. Choose assertion, what characteristic is correct for precitation test : antigens are cells antigens are molecular * for a title accept the most parallel of serum before perform the test it follows to warm up a serum a heterospecific antigen is always parallel used

13. With the purpose to find in the tissues microsection of the fixed autoantibodies it is possible to use a test: A. direct fluorescencent-antibody test B. indirect fluorescencent-antibody test* C. latex-agglutinations D. precipitation in agar E. agglutinations 14. Presumption slide agglutination test is used at beginning of serological authentication . At a positive result for complete authentication Grubbers test is used. What its components? A. Patients serum, diagnosticum B. Unknown antibodies and a known antigen C. Successive dilutions of specific serum and probed culture of bacteria* D. Successive dilutions of patients serum and probed culture of bacteria E. Successive dilutions of the probed bacteria culture and specific serum 15. Explain that it is possible to discover with the help of direct Coombs test: A. antibodies in patients serum B. haptens on the red blood cells C. incomplete antibodies on the red blood cells* D. incomplete antibodies in patients serum E. antibodies on the tissue

16. The Manchini test is there in the figure. What do we dilute in the well? A. Patients tissue B. Bacterial pure culture C. Bacterial suspension D. Patients serum* E. No correct answer

19. What immunological reaction is demonstrated on a picture? A. Agglutination Precipitation C. The double immunodiffusion* D. The radial immunodiffusion Latex-agglutination

17. Results of raising, what reaction presented on a picture? A. Latex-agglutination B. Agglutination C. Coagglutination D. Complement fixation* E. Precipitation

20. Raising, what reaction showed on a picture? A. Reverse indirect hemagglutination B. Inhibition hemagglutination* C. Indirect hemagglutination D. Indirect Coombs test E. Hemagglutination

18. Chart, what immunological reaction showed on a picture? A. Grubers agglutination test B. Coagglutination test C. Agglutination test* D. Latex-agglutination test E. Indirect Coombs test

21. Components, mechanism and account of results of the Groobers agglutination test for identification of microorganisms. (3) 22. Components, mechanism and account of results of precipitation test in a gael for the determinating of antigens. (3) 23. Components, mechanism and account of results of reaction of indirect hemagglutination test for serological diagnostics of bacterial infections. (4)

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