1

A group of student carried out a experiment to show the effects of varying the temperature on the rate of
an enzyme-catalysed reaction. They used amylase as the enzyme and starch as the substrate. Mixtures of
amylase and starch suspensions were immersed in water baths set at 5 oC as shown in diagram 1.1
At every two minutes interval, a drop of the mixture was transferred onto a tile containing iodine solution.
The step was repeated for mixtures at 28 oC, 37 oC, 45 oC, and 60 oC. Table 1 shows the colour of the
iodine solution in the tiles at different temperatures.

Diagram 1

Temperature

Observation
Colours of iodine solution

5 oC

28 oC

37 oC

45 oC

60 oC

Table 1

Time taken (min)

(a) (i) Based on the Diagram 1, state two different observations made from this experiment.
Observation 1
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Observation 2
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(3 marks)
(ii) State inference which corresponding to the observation in 1 (a)(i).
Inference from observation 1
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Inference from observation 2
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(3 marks)
(b) Record the time taken in the boxes provided in Table 1 for each temperature when the mixture fails to turn
blue-black when tested with iodine solution.
(3 marks)
(c) Complete Table 2 based on this experiment.
Variable

Method to handle the variable

Manipulated variable :
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Responding variable :
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Constant variable :
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Table 2
(3 marks)
(d) State one suitable hypothesis for this experiment.
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(3 marks)
(e) (i) Construct a table and record all the data collected in this experiment.
Your table should have the following titles:
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Temperature
Time
Rate of reaction
(3 marks)

(ii) Use the graph provided on next page to answer this part of question. Using the data in 1(e)(i), draw the
graph of the rate enzyme reaction against temperature.
(3 marks)

Rate of enzyme reactions against temperature.

(f) Based on the graph in 1(e)(ii), explain the relationship between the rate of reaction, which is catalysed by
amylase and the temperature.
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(3 marks)
(g) Based on the result of this experiment, explain the meaning of enzyme.
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(3 marks)
(h) The experiment is repeated by adding lime juice to the mixture of amylase and starch. Predict what will
happened to the colour of iodine and why?.
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(3 marks)
(i) In another experiment, pepsin, egg albumen, 0.1M hydrochloric acid, distilled water and 0.1M sodium
hydroxide solution are used. Classify these materials according to their function in the experiment. Fill in
the table below :
Materials

Function

(3 marks)

Microorganisms are very small organisms that can only be seen clearly under a microscope. The activity of
microorganisms is affected by abiotic components such as concentration of nutrients, light intensity, pH and
temperature of the surroundings.

Design a laboratory experiment to study the effect of temperature on the activity of yeast. The planning of
the experiment must include the following aspect :
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Statement of identified problem

Objective
Statement of hypothesis
Variables
List of apparatus and materials
Technique used
Experimental procedure
Presentation of data
Conclusion
(17 marks)

END OF QUESTIONS PAPER