Create 3D mosaics by stitching together image stacks from confocal, brightfield, and fluorescent microscopes.

Automatically or manually combine multiple image stacks to create a single composite image for analysis. Full integration with the Zeiss ApoTome 2 for confocal structured illumination microscopy. The leading cost-effective system for acquiring and analyzing multichannel images of neurons. Neurolucida can now create Virtual Tissue (focusable 3D virtual slides). Improved acquisition of 2D and 3D virtual slides. A new focus map and live preview makes creating focusable 3D virtual slides even better. Use Neurolucida to analyze 2D and 3D virtual slides and image stacks over the web using the new Biolucida Cloud. Collaborators from your department, your institution and around the world can view and analyze all your image data using Neurolucida. . . right from their labs. Automatic Live Focus for tracing dendrites and axons. New analyses: Node count, spine count, and spine class count are in the Layer Length Analysis. New analyses: Segment Point Analysis in the Branched Structure Analysis. Image files can be associated with sections. Helps to create better anatomical maps using virtual slides. Serial Section Manager aligns field of view placement when moving from section to section in virtual slides. Fully integrated image deconvolution within Neurolucida using the renowned Huygens technology. Increases image clarity for more effective analysis and increases accuracy of AutoNeuron. Batch image deconvolution. Performs unattended image deconvolution on multiple stacks. Improved automatic 2D object detection (counting) and tracing (mapping) on virtual slides. Allows you to automatically count and trace cells in entire virtual slides.

New interactive guided-tracing method for 2D and 3D. Increases throughput for hard-to-trace neurons and gives unprecedented accuracy in automated neuron tracing. Improved accuracy for tracing branch thickness (using super ellipsoids). Improved seed visibility. Seeds easier to see in 2D and in 3D. Improved accuracy for automatically tracing golgi stained neurons. More real-time interaction for setting detection parameters.

Integrates fully with your Zeiss Laser Scanning Microscope equipped with Zen software. Integrates with most confocal microscopes. Acquire and analyze 3D images with Zeiss, Olympus, Nikon & Leica laser scanning confocal microscopes. Allows easy creation of 3D montages of images and image stacks from virtually any confocal microscope.

Weve incorporated dozens of suggestions from users around the world to make Neurolucida the fastest, most accurate, and easiest-to-use neuron morphometry solution in the world. Improved image management, including selection and adjustment of multiple images. Easier and quicker operation, processing hundreds of images simultaneously. Selectively brighten images in a particular area or optimize images from a previously dark image acquire.

New and improved interface for image processing features. Crop image stacks in all three dimensions (X, Y, and Z). Resize/resample images and image stacks. Display and save them in a desired publishable DPI. Make and save screenshots that combine images and tracings. Save the max and min projections from a 3D virtual slide. Extract and write out individual planes of a 3D virtual slide. Collect extended luminance data with contours, including calculations for the individual channels as well as un-weighted and weighted calculations for true color images. Export individual channels of a stack to separate image files using the JPEG 2000 file format. Improved support for multimodal imaging that allows a mix of fluorescent, confocal, and brightfield channels. Individually optimize the white and black point of each image in a stack to compensate for photobleaching and light attenuation. New tools for acquiring images with differential brightness across sections for virtual slides and image stacks. Use the macro view to select your image in the image organizer. Improved 3D visualizations, including better tree transparency controls when viewing tracings and image stacks simultaneously. Easily extract multiple serial sections from a single virtual slide image. Added glycerin as an immersion medium to the scaling and lens-related dialogs. Manage the settings for multiple users of the microscope system. Core facility and lab administrators can save and push settings to simplify system configuration.

Improved support for dual camera systems. Consistent orientation for easier use of multi-camera systems, including QImaging, Hamamatsu, and Zeiss AxioCam cameras. Control two shutters using the open shutter/close shutter button on multichannel control dialog. Support for Photometrics cameras. Support for cameras that use the DCAM interface protocol. Support for directly controlling Optronics cameras using PictureFrame3 on 32-bit Windows 7. 16-bit pixel support for Hamamatsu Orca camera. Support for Zeiss ApoTome 2 automatically change grids. Support for Zeiss MTB-controlled stages. Support for Olympus BX-63 motorized microscope stand. Support for LEP absolute focus encoder. Support for Lumencor Sola illumination device. Support for the Sutter Lambda, EXFO X-Cite 120PC, and exacte Fluorescence Illumination Systems. Support for Olympus OIB/OIF files. Enhanced support for the Nikon IDS/ICS format. Support for newest version of Aperio SVS file format. Improved support for reading and writing XML files.