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NORWEGIAN UNIVERSITY OF LIFE SCIENCES

Department of Chemistry, Biotechnology and Food Science

Team 4 Lignocellulose to biofuels

Topic: enzyme technology for conversion of lignocellulosic biomass

www.umb.no

NORWEGIAN UNIVERSITY OF LIFE SCIENCES

Department of Chemistry, Biotechnology and Food Science

The biorefinery, 2nd generation biofuels and enzymes Notes


Enzymatic deconstruction is the method of choice. Enzymes are a major cost.

Biofuel is only one of many possible products.

www.umb.no

NORWEGIAN UNIVERSITY OF LIFE SCIENCES

Department of Chemistry, Biotechnology and Food Science

UMN-UMB, Team 4 specific project: CBP21, a helper protein from the CBM33 family

Gustav Vaaje-Kolstad et al., Journal of Biological Chemistry 280: 11313 11319 & 280:28492-28497 (2005) + Patent application www.umb.no

NORWEGIAN UNIVERSITY OF LIFE SCIENCES

UMN-UMB, Team 4 specific project starting point


Goals: Use of directed evolution (mutagenesis) to produce accessory proteins that act on cellulose. Generation of fundamental knowledge about how helper proteins such as CBP21 work.

People:
Claudia Schmidt-Dannert group with post-doc Jake Vick. Eijsink group with post-doc Gustav Vaaje-Kolstad and (since 2010) Ph.D. student Zarah Forsberg.

www.umb.no

NORWEGIAN UNIVERSITY OF LIFE SCIENCES

UMN-UMB, Team 4 specific project Developments since 2007

GH61 proteins act synergistically with cellulose and show structural similarity with CBM33 (CBP21) CBM33 proteins are enzymes that break down chitin chains GH61 proteins are enzymes that break down cellulose chains Identification of natural CBM33 proteins that break down cellulose

Interesting ideas and findings at UMN concerning mechanism

CBM33
www.umb.no

GH61

A new paradigm for degradation of crystalline polysaccharides

NORWEGIAN UNIVERSITY OF LIFE SCIENCES

Department of Chemistry, Biotechnology and Food Science

Endo

Exo-processive

+
www.umb.no

Oxidohydrolase
(CBM33 or GH61), catalyzing chain cleavage in a fully crystalline context
Vaaje-Kolstad et al., 2010, Science 330:219-222

NORWEGIAN UNIVERSITY OF LIFE SCIENCES

UMN-UMB, Team 4 Status June 2011


Very hot project. IP issues. Lots of mutagenesis work on CBP21 has been done and mutant characterization is in progress. Several new, active CBM33s available. NMR structure of CBP21 (a CBM33) has been solved. Shift of focus from enzyme development to enzyme understanding. Several joint papers, including potential breakthrough papers on mechanism, are on their way. Funding is running out (?)

www.umb.no

NORWEGIAN UNIVERSITY OF LIFE SCIENCES

Department of Chemistry, Biotechnology and Food Science

Notes about the future


Funding ? (currently only one PhD student at UMB)

Many applications sent in Norway (but)


MSc student exchange: Sophanit Mengesha PhD student exchange: Zarah Forsberg (?)

Nb. Current potential is huge and progress is good, but very high complexity (protein production, analytical tools, theoretical biochemistry, many partners).

www.umb.no

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