Beruflich Dokumente
Kultur Dokumente
Jos A. Teixeira
IBB - Institute for Biotechnology and Bioengineering, Centre of Biological Engineering University of Minho, PORTUGAL e-mail: jateixeira@deb.uminho.pt
Sugarcane
Brazil, India
Corn
Sugar beet
Europe (France)
Wheat
Sorghum
India
Cassava
Cheese Whey
New Zealand
40%
89%
60%
Sugar crops
Starch crops
Vehicles use ethanol in the pure form or in mixture with the gasoline, where ethanol corresponds up to 25% of the mixture Ethanol is used in two forms: mixed with gasoline in the maximum proportion of 10%, or in mixtures containing 85% ethanol and 15% gasoline, as an alternative fuel Current blend levels are 10%, but efforts are being directed to expand ethanol blends to 25% Gasoline must be blended with 10% ethanol Requires 10% ethanol blends in cities with populations over 500,000 Some regions of China use mixtures containing up to 10% ethanol in gasoline Addition of 5% ethanol to gasoline is mandatory. Efforts will be directed to increase the ethanol percentage in the mixture to 10% 5% of all motor vehicle must be ethanol or biodiesel Mixtures containing 5% ethanol in gasoline are used The replacement of 3% of gasoline by ethanol is authorized, but efforts will be done to increase this value to 10%
Rice straw
LIGNIN
HEMICELLULOSE
- Arabinofuranose
H H OH H HO O
H
Acetyl group
O CH3 O
H H H H O H OH
Xylobiose
H O H OH H H
H H OH H H O
H H OH H H
OH
H H O H H H OH
H H H OH H H O
H
O
H H O H OH H H OH
H
O
H H OH H H O
O H OH H H OH
O H OH H H OH
OH
OH
OH
O
O CH3 H3 C
O
O
H
OH
O
H3C
O
H OH H H OH
H OH H H OH
Acetyl group
Glucuronic acid
Glucuronic acid
OH OH HO O
HO
OH O OH OH HO
O O
O OH
OH HO
O
HO
O OH
HO
O OH
OH
Cellobiose
Fermentation of sugars
Pre-treatment
Milling
Iogen (straw Canada); Abengoa (straw Spain, US); Etek (softwood Sweden); Elsam (straw Denmark); TMO (straw etc. UK); Tavda (wood Russia); NEDO (rice straw Japan)
(1)
(3)
Cellulose fibers
(2)
Specific enzymes
Glucose
CELLULOSE
fermentation
3) Other important considerations for the process implementation To develop microorganisms able to metabolize pentose and hexose sugars simultaneously withstanding the stress imposed by the process inhibitors To evaluate the process scalability
Sweet potato
Sweet corn
Jerusalem artichoke
Sweet sorghum
Carbohydrate and expected ethanol yields for sweet corn, Jerusalem artichoke, sweet potato ad sweet sorghum Current ethanol yield from grain corn in the US and sugarcane in Brazil is approximately 3,500 and 6,000 L/ha, respectively.1
It
It
more that of C3 crops (forest) is able to grow anywhere in dry climates with high yields of fermentable sugars,
In some
It
regions it is possible to obtain two plantations per year reaching full maturity
and a large production. has low water requirements 1/3 of sugar cane, 1/2 of corn, 1/4 of Short Rotation
Forestry.
Sweet
Miscanthus x giganteus properties relatively high yields 8-15 t/ha (3-6 t/acre) dry weight, low moisture content (as little as 15-20% if harvested in late winter or spring), annual harvests, providing a regular yearly income for the grower, good energy balance and output/input ratio compared with some other
biomass options,
low mineral content, especially with late winter or spring harvest, which improves fuel quality. can be grown in a cool climate like that of northern Europe
typical acre of corn yields around 7.6 tons of input per acre and
switchgrass,
giant
Bioconversion of lactose to ethanol represents a process which can provide a value-added product from cheese manufacturing, allied with efficient bioremediation of plant effluent.
Represents 8595% of the milk volume and its world production is estimated to be over 108 tons/ year Lactose (5-6% w/v) is assumed to be responsible for 90% of the wheys BOD and COD.
Liquid remaining after the precipitation and removal of milk casein during cheese-making
CHEESE WHEY
Fermentation of sugars
Ethanol
Direct fermentation of whey or whey permeate to ethanol is generally not economically feasible because the low lactose content results in low ethanol titre (23% v/v), making the distillation process too expensive.
Cheese whey concentration: by ultrafiltration and/or reverse osmosis processes. Yeasts that ferment lactose: Kluyveromyces lactis, K. marxianus, Candida pseudotropicalis, genetically modified Saccharomyces cerevisiae.
Whey to bioethanol
8 million tons of lactose (worldwide annual whey production)
sub-products
since 1978, potable ethanol & ethanol for fuel (since 2005) 11 000 tons ethanol /year
New Zealand
Fonterra
United States
Germany
Mllermilch
A Saccharomyces cerevisiae strain that efficiently metabolizes lactose was developed, with a lactose metabolization capacity comparable to the one presented by other natural lactose users
A high ethanol productivity (10 gl-1h-1) system using cheese whey as a substrate was developed
system
Fermentation of Cheese Whey powder solutions by T1-E 110150 gL-1 Lactose + Corn Steep Liquor (10 gL-1)
Repeated-batch operation with biomass recycling by flocculation
-1
CO2
The microalgae Chlorella vulgaris, particularly, has been considered as a promising feedstock for bioethanol production
Water
Water
nutrients
nutrients
Enzymatic
Fermentation
Starch
Microalgae cultivation Cell rupture
hydrolysis
sugars
ETHANOL
microalgae can be harvested batch-wise nearly all-year-round they grow in aqueous media, but need less water than terrestrial crops, therefore reducing the load on freshwater sources the ability of microalgae to fix CO2
Source Corn stover Wheat Cassava Sweet sorghum Corn Sugar beet Sugarcane Switch grass Microalgae
Ethanol yield (gallons/acre) 112-150 277 354 326-435 370-430 536-714 662-802 1,150 5,000-15,000
Ethanol yield (L/ha) 1,050-1,400 2,590 3,310 3,050-4,070 3,460-4,020 5,010-6,680 6,190-7,500 10,760 46,760-140,290
Conclusions
Corn is the main raw material used today, but in the future.....
Dont affect the food provision Microalgae Cheese whey Lignocellulose Agave