INFLUENCE OF HYPERACTIVITY TREATMENT AT THE COLLAGENOUS TISSUE DENSITY OF THE MUSCLE ATTACHMENT OF LONG BONES DURING GROWTH

NAME : ARIE PRADIPTA NIM : 030.07.031

TOPIC Collagen is the major structural protein of the body

Approximately 33 percent of protein in the body is collagen In fact, bone is also composed of collagen combined with certain minerals such as calcium and phosphorus Bone is a tissue composed of cells and is dominated by collagen extracellular matrix (type I collagen) are known as osteoid.

METHODS A method for this study building on experimental animal with cross-sectional approach During the migration process, there are changes in the density collagen tissue As experimentals animal used 120 male rats - Age 6 weeks - Weighing 70 grams Doing the experiment in hyperactivity treatment during 2 until 6 months, may be used to explain the results of primary research

METHODS Preparations for this experiment taken from musculus pectineus and musculus rectus femoris from experimental animal, that is the muscle attachment of the long bone during growth being checked under microscope Preparations workable by the method of paraffin and made cuts nearly parallel to the axis bone length and preparations 6 microns thick slices and polished with Azan Mallory staining On microscopic preparations of m. pectineus and m. rectus femoris attachment assessed the level density of collagen tissue is qualitatively by comparing the color of the collagen tissue with the guidelines previously determined color density values with degree of color intensity values, ie less dense (+) with a score of 1 quite dense (++) with a score of 2 the densely (+++) with a score of 3, drawn from the entire preparation.

METHODS Three example of the level density can be seen in the picture below:

Figure 1: Microscopic images of longitudinal cross section of the muscle tendon attachment on the bone density that show fibrae collagenous less dense. Mallory azan staining. Information: 1.Fibrae collagenouse 2. Cartilage cells

METHODS Three example of the level density can be seen in the picture below:

Figure 2: Microscopic images of longitudinal cross section of the muscle tendon attachment on the bone density that show fibrae collagenous pretty solid. Mallory azan staining. Information: 1.Fibrae collagenouse 2. Cartilage cells

METHODS Three example of the level density can be seen in the picture below:

Figure 3: Microscopic images of longitudinal cross section of the muscle tendon attachment on the bone density showed a hiper density fibrae collagenous. Mallory azan staining. Information: 1.Fibrae collagenouse 2. Cartilage cells

METHODS The Implementation Of The Research All of the experimental animals are placed to live inside the cage with 90cm long and 30cm height of size, for 10 rats in about 2 until 6 months after treatment Rats are fed with food 521 and added with 100gr mashed corns each day for every 10 rats and also drink ad libitum Experimental animals are divided in to two groups: 1. Controls (60 rats) 2. Hiperactivity (60 rats) The value of the collagen tissue density is taken at the M.pectineus attachment on femur proximal part and M.rectus femoris on tibia proximal part, on the control group and hyperactivity group, each after 2 months and 6 months of treatment on the both group mentioned before

METHODS Measurement Result Of The Collagen Tissue Density On The Muscle Attachment To The Bone Table XIIa: The average of the collagen tissue density on control group 2 months and 6 months Time of Treatment The Average of Density 2 months Collagen Tissue 6 months

Muscles Attachment
M.pectineus M.rectus femoris

S.B

S.B

1,567 2,600

0,496 0,554

1,933 2,833

0,249 0,373

METHODS Table XIIb: The average of the collagen tissue density on hyperactivity group 2 months and 6 months Time of Treatment The Average of Density Collagen Tissue

2 months
Muscles X S.B X

6 months
S.B

Attachment
M.pectineus M.rectus femoris 1,967 2,700 0,180 0,458 2,000 2,767 0,000 0,423

METHODS Table XIIc: Result of Fisher Exact test from the differences of the collagen tissue density between 2 months and 6 months

Group

Control

Hyperactivity

Muscles Attachment M.pectineus p>0,05*

*the differences not to shown

METHODS Table XIIc: Result of Chi-kuadrat test from the differences of the collagen tissue density between 2 months and 6 months Muscles Attachment Group M.pectineus X2=10,755 p<0,01** M.rectus femoris X2=3,484 p>0,05*** X2=17,818 p>0,05*** Control Hyperactivity

*the differences shown **the differences very shown ***the differences not to shown

RESULT Hyperactivity treatment causes collagenous tissue density show fibrae collagenous less dense, pretty solid, and hiper density The addition of muscle activity during the development will increase the growth of tendon. In hyperactivity treatment (physical exercise) there is a correlation between collagenous tissue density and the muscle attachment of long bones was significantly with the degree of migration of muscle attachment on the bone during growth.

RESULT The treatment makes collagenous tissue density show fibrae collagenous: Less dense Pretty solid Hiper density Assessed the level density of collagen tissue is qualitatively by comparing the color of the collagen tissue with the guidelines previously determined color density values with degree of color intensity values, ie 1. less dense (+) with a score of 1 2. quite dense (++) with a score of 2 3. the densely (+++) with a score of 3, drawn from the entire preparation. In the hyperactivity treatment was found that the absolute color density values with degree of color intensity values of the collagen tissue density at the muscle attachment

CONCLUSION The influence of hyperactivity treatment for the experimental animal to get the changes of collagenous tissue density of the muscle attachment of long bones during growth. It was found that in the hyperactivity rats, a change of collagenous tissue density was detected, whereas none in the control group. The change of the collagenous tissue density show fibrae collagenous less dense, pretty solid, and hiper density. In conclusion, there was a convincing the influence of hyperactivity treatment at the collagenous tissue density of the muscle attachment of long bones during growth. That influence to the muscules gain mass and will add the sacroplasma. Hyperactivety treatment causes muscle hyperplasia or hypertrophy that increases muscle volume and bone length change.

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