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The goal of molecular cloning is large amounts of pure DNA that can be further manipulated and studied. Restriction Enzymes each recognize a specific DNA sequence (restriction site), and break a phosphodiester linkage between a 3' carbon and phosphate within that sequence. Restriction sites in the bacterial chromosome are methylated, and thus protected.
The goal of molecular cloning is large amounts of pure DNA that can be further manipulated and studied. Restriction Enzymes each recognize a specific DNA sequence (restriction site), and bre…