Beruflich Dokumente
Kultur Dokumente
Colony morphology
1. Colony shape and size: round, irregular, punctiform (tiny) 2. Margin (edge): entire (smooth), undulate (wavy), lobate (lobed) 3. Elevation: convex, umbonate, flat, raised 4. Color: color or pigment, plus opaque, translucent, shiny or dull 5. Texture: moist, mucoid, dry (or rough).
Bacillus
Proteus
Staphylococcus
Streptococcus
MacConkey Agar
Purpose MacConkey agar is a widely-used culture medium which is both selective AND differential. The medium is primarily used to differentiate between Gram negative bacteria while inhibiting the growth of most Gram positive bacteria. The medium also differentiates between lactose-fermenting coliforms and lactose nonfermenters, which include potential pathogens. Principle Addition to the nutrient agar base of bile salts and crystal violet will inhibit the growth of most Gram positive bacteria, making MacConkey agar selective. Lactose, a fermentable carbohydrate, and neutral red, a pH indicator, are added to differentiate the lactose positive coliforms from the potentially pathogenic lactose nonfermenters. Additional Information When lactose is fermented, acid products lower the pH below 6.8, with the resulting colonial growth turning pinkish-red. If an organism is unable to ferment lactose, the colonies will be colorless.
Mac continued
Staphylococcus aureus