VCE Chemistry Unit 3 2013 Chapter 7 UV-Visible Spectroscopy

Week commencing 18th Feb Ms Lennard

Overview
Chapter 7 spectroscopy The electromagnetic spectrum UV-visible spectroscopy Theory for SAC Prac #1 Determination of the P content of lawn food

Chapter 7 - spectroscopy
We will study AA after we have completed theory for the 1st SAC (EEI) We will complete and IR, NMR after we have completed Chapter 9 (organic chemistry)

keywords
Electromagnetic spectrum UV (ultra violet) visible spectroscopy Colorimetry (visible spectroscopy) Calibration curve Monochromator Absorbance Transmittance Spectrum photon

wavelength

Electromagnetic spectrum

Electromagnetic spectrum
http://www.youtube.com/watch?v=kfS5Qn0w n2o

spectroscopy

UV-Visible spectroscopy
Measures the light absorbed by substances as their electrons jump to higher energy levels The colour of a solution is the result of the light transmitted by that solution after certain components of visible light have been absorbed through electron transitions
A coloured substance absorbs its complementary colour eg. A solution appears yellow because the blue and violet light has been absorbed and the transmitted light is yellow

UV-Visible spectroscopy
The position of a peak in a UV-vis spectrum shows the energy of the light most strongly absorbed by that substance The amount of light absorbed is directly proportional to the concentration of the compound A calibration curve (generated from the absorbance of samples of known concentration) is used to determine the concentration of the compound UV-vis spectroscopy is used to determine concentration of solutions of coloured compounds

Beer - Lambert Law

Visible light and complementary colours

Visible light

UV - vis

UV- vis spectrophotometer

faculty.sdmiramar.edu

www.snypa.co.uk/OSR/UV/Resources/UV_spec.jpg

http://www.youtube.com/watch?v =0n-dbLzj_HM
You tube UV/Vis Spectroscopy

Visible spectrum of the green pigment chlorophyll

Using UV-Vis spectrum to choose the wavelength for analysis choose a wavelength where the substance absorbs strongly eg. 420nm or 660nm

Calibration curves
Enable unknown concentrations to be determined by comparing with known standards (of concentration) A series of standard solutions are tested using the equipment under the same conditions The response to these standards is measured and plotted to form a calibration curve (which is usually a straight line) The measurement of the unknown sample is plotted on the same curve and its position is used to find its concentration Calibration curves are used in AA and UV-Vis spectroscopy

Calibration curves

Worked example 7.4 p. 87

Worked example 7.4 p. 87

Questions p. 88
Q6, 7, 9, 10, 11, 12

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