DOAST- NPK- Chennai- INDIA

DOAST Integrated Therapy

Successful in-vivo technique in synergy with Global research. Treatment Guided research which
Follows the LEADS and focuses on safe and effective interventions
DOAST- NPK- Chennai- INDIA
2

Dr.Bernaud Rimland Authored the Neurobiological approach. The First

POSITIVE TURN IN AUTISM RESEARCH

Four decades of Global Research. Are we able to deliver peace at home?
DOAST- NPK- Chennai- INDIA
3

 Autism research exhibits analytical

threads, but patterns remain fragmented lacking a unified concept. As on date we lack a coherent view on brain function within the framework of other organ systems.

DOAST- NPK- Chennai- INDIA

POSITIVE APPROACH of DOAST Instead of studying it through a symptom by symptom approach, autistic behavior should be understood better by studying the underlying intermediary biological processes
DOAST- NPK- Chennai- INDIA
5

Emerging qualitative changes in clinical status including loss of diagnosis suggest that a more appropriate description for ASD could be a chronic dynamic reversible encephalopathy rather than static encephalopathy as considered so far.
DOAST- NPK- Chennai- INDIA
6

Cognitive, emotional and synaptic functions of the brain basically rely on the macromolecular computing system of RNA protein- and protein to protein interactions which appear to be the result of fine grained information, encoded and accumulated over evolutionary time.

DOAST- NPK- Chennai- INDIA

DOAST- NPK- Chennai- INDIA

Neuro-Endo-ImmuneGI Tract Network, Autonomic Balance, &Neurotransmitters.

DOAST- NPK- Chennai- INDIA
9

Genes, Molecular order, Protein expression, Nerurotransmitters Neuronal connectivity.

CNS-ENDO-IMMUNE-GI Tract- Network Homeostasis

Bio feed back & Autonomic Balance

Upstream molecular unrest

Altered network Homeostasis

Normal Performance Social&Cognitive, Activities

Downstream Systemic Inflammation

Behavior&Cognitive dysfunction.

AUTISM

Susceptible Genetic landscape

DOAST- NPK- Chennai- INDIA

10

Decreased connectivity is at the heart of autism. Fairly close regions of the brains involved in motor tasks have difficulty coordinating activity. It makes sense that social and communication skills are greatly impaired, as they involve more complex synchronous coordination between more distant areas of the brain.
DOAST- NPK- Chennai- INDIA
11

Consequences of inflammatory factors Breaking boundaries between the body and brain, Destabilization and loss of synaptic connections.
DOAST- NPK- Chennai- INDIA
12

Our Concept..
Down stream inflammation
Dietary or environmental triggers, Gut micro biome systemic inflammatory cytokines on a chronic basis

Upstream molecular unrest

Synaptic connectivity affected in in susceptible individuals.

13

DOAST- NPK- Chennai- INDIA

Genes, Molecular order, Protein expression, Nerurotransmitters Neuronal connectivity.

CNS-ENDO-IMMUNE-GI Tract- Network Homeostasis

Bio feed back & Autonomic Balance

Restore Network Homeostasis

Normal Performance Social&Cognitive, Activities

Upstream molecular order

Downstream Molecular order

INTEGRATED THERAPY

Improved Behavior& Cognition

Integrated Therapy Hypothesis
DOAST- NPK- Chennai- INDIA

Cellular Stressors
14

Deviate from the Hard wired concept and untreatable stigma. Effectively treating the downstream inflammatory pathology could initiate reestablishment of upstream molecular order . Molecular order restored could activate the dormant neuronal pools, improve connectivity and restore functional integration.

DOAST- NPK- Chennai- INDIA

15

DOAST Integrated therapy

Pioneering concept of therapeutic integration or fusion, where scientifically chosen traditional Indian treatment methods are applied with modern medical understanding to treat specific chronic ailments, and autism in particular.

DOAST- NPK- Chennai- INDIA

16

The Components of Integrated Therapy.

The Science of Yoga, Ayurveda & Siddha & Modern medical expertise.

DOAST- NPK- Chennai- INDIA

17

Reducing Cellular stress Toxin elimination Rejuvenation and Regeneration.

DOAST- NPK- Chennai- INDIA
18

Prognostic Evaluation
No reliable bio-marker. Observed improvements in behavior and cognition domains are the only reliable non-invasive indicators of restoration of molecular order and integration of the upstream neuronal pools.
DOAST- NPK- Chennai- INDIA
19

Patient selection. DSM-IV guidelines, (CARS) and M-CHAT are the standard assessment tools used.

Patient pool represents a spectrum of severity observed in autistic patients in general. Their racial origin is Asian. Age 3- 16 years.
Cohort of 30 individuals on different follow up period on this therapy since 2004.

DOAST- NPK- Chennai- INDIA

20

Therapeutic protocol
The prescribed diet Sathvik Diet practiced by

the science Yoga. Herbal medications, certified by the Ayurvedic [including Siddha] pharmacopia of India are administered. Yoga technique is a combinatorial approach. Oleation therapies with medicated oils.

DOAST- NPK- Chennai- INDIA

21

Ethical & safety guidelines followed

A well informed consent, The therapy techniques and medicines are chosen

by evidence based risk benefit ratio, proven over centuries in their clinical application, One to one & Group basis application along with the parent, Documentation on paper and video, Pretreatment EEG and periodic monitoring of biochemical parameters to assess renal, hepatic and hematological functions.
DOAST- NPK- Chennai- INDIA
22

Assessment of Clinical benefits

GI Tract, Respiratory, Sleep, Morbidity, Emotions, Behavior, Cognition.
Eye contact, Play, Communication, Empathy & Generalization are the behavior domains chosen to assess and quantify behavior and cognitive improvement.

DOAST- NPK- Chennai- INDIA

23

Controls
We follow each patient, from the time of admission and

through their recovery (or lack of it). Thus each participant in the study will serve as his/her own control.
Improvement is influenced by the age of induction into

therapy, duration of follow-up and compliance to the details.

Parental compliance to be proportional to the trust on therapy. Willful or accidental default in medicine or dietary regime 'influenced by the above listed variables', resulted in temporary return of symptoms. Such events helped us understand the therapeutic role played by components of therapy in a better way.
DOAST- NPK- Chennai- INDIA
24

VALIDITY Consistent replication lends validity, Supports the hypothesis that chronic inflammation could indeed be the common denominator.
Future research We contemplate generating carefully characterized full genome expression profiling data to validate our biomedical approach and to study the involvement of the intestinal flora in the inflammation related etiology of autism.
DOAST- NPK- Chennai- INDIA
25

The core of our study will be a repeated measures examination of the interplay between the biology of inflammatory and oxidative stress markers on the one hand and processing, connectivity and reactivity abnormalities on the other which may conceivably converge on to the autism profile.
DOAST- NPK- Chennai- INDIA
26

Proposed Study
Examine the gastrointestinal

metagenomic analysis of intestinal flora, Non-coding RNA (ncRNA) expression profiles on lymphoblasts in a series of before and after comparison based molecular studies.
DOAST- NPK- Chennai- INDIA
27

Outcome of the proposal

Generate direct evidence in favor of a causal relationship between systemic inflammation and autism, Generate diagnostic determinants for definition of this variant of autism, Define a link between diagnostics and treatment by this and any future treatment technique.

DOAST- NPK- Chennai- INDIA

28

In the least invasive manner, using this DOAST treatment modality as a physiological probe, we study the linear, causal relationship between cellular-level changes and behavioural problems within the high performance environment of the human nervous system.

DOAST- NPK- Chennai- INDIA

29

The identification of relationships between the multiple levels, all of which can be expected to shift based on both huge amounts of clinical experience and a growing wealth of published scientific data, will be a contribution to identifying treatment targets that simultaneously address multiple features of autism at their root.
DOAST- NPK- Chennai- INDIA
30

While there are risks associated with any dogma challenging proposal, the most compelling gains of this project is that success in any of the multiple facets of this investigation will lead to important new knowledge about epigenetic and molecular denominator based integrated therapeutic approaches for a heterogeneous neural disorder that has so far not responded significantly to mono therapy.

DOAST- NPK- Chennai- INDIA

31

DOAST- NPK- Chennai- INDIA

32