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Knowing is not enough; we must apply. Willing is not enough; we must do. (Goethe)
Educational Objectives
Outline the epidemiology of viral hepatitis List causative agents for viral hepatitis Recognize the clinical features of acute and chronic viral hepatitis and their complications Interpret serologic tests to accurately diagnose the specific cause of viral hepatitis Identify appropriate candidates for vaccination against HAV and HBV
Type of Hepatitis
A
Source of virus feces
E
feces
blood/ blood/ blood/ blood-derived blood-derived blood-derived body fluids body fluids body fluids percutaneous percutaneous percutaneous permucosal permucosal permucosal
Route of transmission
fecal-oral
fecal-oral
Chronic infection
Prevention
no
yes
yes
yes
no
pre/postexposure immunization
pre/postexposure immunization
blood donor pre/postscreening; exposure risk behavior immunization; modification risk behavior modification
Acute HAV mostly symptomatic, especially in adults Acute HEV is most symptomatic and severe in pregnant women Acute HCV is least symptomatic Acute HBV can present with a serum sickness-like picture (fever, arthritis, urticaria, angioedema) Generally, symptoms improve after jaundice appears Symptoms are non specific-the viral syndrome (fever, malaise, anorexia, RUQ pain, diarrhea, pruritis)
Acute Hepatitis-LAB
Markedly elevated levels of AST/ALT (more than 500 U/L) typically occurs in acute hepatocellular injury (viral, drug-induced, ischemic) Modest elevations (less than 300 U/L) may be seen in a variety of conditions
chronic hepatitis infiltrative diseases biliary obstruction
Bilirubin and AP may or may not be elevated WBC count may show leucopenia
Spider Agiomatas Palmar erythema Clubbing of fingers Dupuytren contractures Gynecomasita (male) or breast atrophy (female) Testicular atrophy
Hepatitis A
Clinical Features
Average 25 days Range 15-50 days <6 yrs, <10% 6-14 yrs, 40%-50% >14 yrs, 70%-80% Fulminant hepatitis (0.1 %) Prolonged Cholestasis ? Autoimmune hepatitis None
Hepatitis A Infection
Typical Serological Course
Symptoms
Total anti-HAV
Total anti-HAV
Titre
ALT
Fecal HAV
IgM anti-HAV
IgM anti-HAV
1 2
2 4
Many cases occur in community-wide outbreaks No risk factor identified for most cases Highest attack rates in 5-14 year olds Children serve as reservoir of infection Persons at increased risk of infection Travelers Homosexual men Injecting drug users
Hepatitis B
Clinical Features
Incubation period: Clinical illness (jaundice): Acute case-fatality rate: Chronic infection: Premature mortality from chronic liver disease: Average 60-90 days Range 45-180 days <5 yrs: <10% > 5 yrs: 30%-50% 0.5%-1% <5 yrs: 30%-90% 5 yrs: 2%-10% 15%-25%
Acute infection
Arthralgias Papular acrodrmatitis (Gianotti-Crosti
syndrome)
Chronic infection
Glomerulonephritis
Arthrlalgias
Polyarteritis nodosa (PAN)
Papular acrodermatitis
PAN
Total anti-HBc
Titre
HBsAg IgM anti-HBc anti-HBs
12 16 20 24 28 32 36
52
100
Titre
IgM anti-HBc
0 4 8 12 16 20 24 28 32 36
52
Years
100 80
80
60
Chronic Infection
Chronic Infection (%)
60
40
40
20
20
Symptomatic Infection
0 Birth 1-6 months 7-12 months 1-4 years 0 Older Children and Adults
Age at Infection
Moderate
semen vaginal fluid saliva
Parenteral - IVDA, Health Workers are at increased risk Perinatal - Mothers who are HBeAg positive are much more likely to transmit to their offspring than those who are not. Perinatal transmission is the main means of transmission in high prevalence populations
Prevention
Vaccination - Vaccine can be given to those who are at increased risk of HBV infection such as
health care workers neonates as universal vaccination in many countries. 3 doses are
Hepatitis B Immunoglobulin - efficacious within 48 hours of the incident. It may also be given to neonates who are at increased risk of contracting hepatitis B i.e. whose mothers are HBsAg and HBeAg positive Other measures - screening of blood donors, blood and body fluid precautions
Hepatitis C Virus
6 different genotypes
capsid envelope protein c22 protease/helicase 33c c-100 RNA- RNA polymerase dependent
5
core E1 E2 NS2 NS3 NS4 NS5
hypervariable region
Hepatitis C
Clinical Features
Incubation period: Clinical illness (jaundice): Chronic hepatitis: Persistent infection: Average 6-7 wks Range 2-26 wks 20-30% 75-85% 85-100%
Immunity:
cryoglobulinemia
Vasculitis
non-deforming arthritis
membranous glomerulonepgritis Porphyria
antiHCV
Titre
ALT
Laboratory Diagnosis
HCV antibody - generally used to diagnose hepatitis C infection. Not useful in the acute phase as it takes at least 4 weeks after infection before antibody appears. HCV-RNA - various techniques are available e.g. PCR and branched DNA. May be used to diagnose HCV infection in the acute phase. However, its main use is in monitoring the response to antiviral therapy.
HCV-antigen - an EIA for HCV antigen is available. It is used in the same capacity as HCV-RNA tests but is much easier to carry out.
Prevention of Hepatitis C
Screening of blood, organ, tissue donors
High-risk behavior modification Blood and body fluid precautions
RNA
Hepatitis E
Clinical Features
Incubation period:
Case-fatality rate:
Average 40 days Range 15-60 days Overall, 1%-3% Pregnant women, 15%-25% Increased with age
None identified
ALT
IgG anti-HEV
Titer
Virus in stool
IgM anti-HEV
1 0
1 1
1 2
1 3
Hepatitis E
Epidemiologic Features
Most outbreaks associated with faecally contaminated drinking water Several other large epidemics have occurred since in the Indian subcontinent and the USSR, China, Africa and Mexico Minimal person-to-person transmission
Chronic
NA
Recovered/latent Vaccinated
Anti-HAV IgG Anti-HAV
IgG
Anti-HBc HBeAG HBV DNA
IgM
IgG
Anti-HBc Anti-HBs
IgG
Anti-HBs
or
HBeAb HCV
All
Anti-HCV Ab
NA
HDV
Anti-HDV
NA
Thank You !
andget vaccinated!!