Phloston

Protein synthesis inhibitors

Protein synthesis inhibitors

30S and 50S inhibitors

Protein synthesis inhibitors

30S and 50S inhibitors
30S = aminoglycosides + tetracyclines

Protein synthesis inhibitors

30S and 50S inhibitors
30S = aminoglycosides + tetracyclines 50S = chloramphenicol + clindamycin + macrolides

(and linezolid = lower-yield, but in FA)

Protein synthesis inhibitors

30S and 50S inhibitors
30S = aminoglycosides + tetracyclines 50S = chloramphenicol + clindamycin + macrolides

(and linezolid = lower-yield, but in FA) Knowing the above classifications is mandatory.

Protein synthesis inhibitors

30S and 50S inhibitors
30S = aminoglycosides + tetracyclines 50S = chloramphenicol + clindamycin + macrolides

(and linezolid = lower-yield, but in FA) Knowing the above classifications is mandatory. However the USMLE is going to throw buzz words at you for these, so you need to know their individual MOAs.

Protein synthesis inhibitors

Before I get into the specifics of each drug, here are the

buzz words you need to associate with the 50S drugs:

Protein synthesis inhibitors

Before I get into the specifics of each drug, here are the

buzz words you need to associate with the 50S drugs: Macrolides = BLOCK TRANSLOCATION STEP via binding to the 23S rRNA of the 50S subunit.

Protein synthesis inhibitors

Before I get into the specifics of each drug, here are the

buzz words you need to associate with the 50S drugs: Macrolides = BLOCK TRANSLOCATION STEP via binding to the 23S rRNA of the 50S subunit. FA says chloramphenicol and clindamycin both block peptide bond formation, but in terms of the USMLE, theyre going to want you to know that chloramphenicol more specifically blocks peptidyl transferase. For clindamycin, it blocks peptide bond formation. Thats how its seen in questions.

Protein synthesis inhibitors

For the 30S buzzwords:

Protein synthesis inhibitors

For the 30S buzzwords:
Aminoglycosides = disrupt initiation complex and

cause misreading of mRNA.

Protein synthesis inhibitors

For the 30S buzzwords:
Aminoglycosides = disrupt initiation complex and

cause misreading of mRNA. Tetracyclines = prevent attachment of aminoacyltRNA to the A-site on the 30S.

Aminoglycosides vs macrolides (names)

Gentamycin, neomycin, amikacin, tobramycin,

steptomycin (highest-yield ones) = AMINOGLYCOSIDES

Aminoglycosides vs macrolides (names)

Gentamycin, neomycin, amikacin, tobramycin,

steptomycin (highest-yield ones) = AMINOGLYCOSIDES Erythromycin, azithromycin, clarithromycin = MACROLIDES

Aminoglycosides vs macrolides (names)

Gentamycin, neomycin, amikacin, tobramycin,

steptomycin (highest-yield ones) = AMINOGLYCOSIDES Erythromycin, azithromycin, clarithromycin = MACROLIDES When you see these drug names enough through doing lots of practice questions, you soon wont have to think twice about their classifications, but at the very minimum, dont screw these up. I had an easy question my real deal where I had to pick out the aminoglycoside, and they had also listed a macrolide.

Bactericidal vs bacteristatic
Youve gotta know which protein synthesis inhibitors

are bactericidal vs static.

Bactericidal vs bacteristatic
Youve gotta know which protein synthesis inhibitors

are bactericidal vs static. The 50S ones alone are BACTERISTATIC. That means the macrolides and chloramphenicol + clindamycin are BACTERISTATIC.

Bactericidal vs bacteristatic
Youve gotta know which protein synthesis inhibitors

are bactericidal vs static. The 50S ones alone are BACTERISTATIC. That means the macrolides and chloramphenicol + clindamycin are BACTERISTATIC. Of the 30S, tetracyclines are BACTERISTATIC, but aminoglycosides are BACTERICIDAL.

Bactericidal vs bacteristatic
Youve gotta know which protein synthesis inhibitors

are bactericidal vs static. The 50S ones alone are BACTERISTATIC. That means the macrolides and chloramphenicol + clindamycin are BACTERISTATIC. Of the 30S, tetracyclines are BACTERISTATIC, but aminoglycosides are BACTERICIDAL. Tangential: TMP and SMX, individually, are bacteristatic. But TOGETHER THEY ARE BACTERICIDAL.

Aminoglycosides
As said before, these inhibit formation of the

initiation complex and cause misreading of the mRNA.

Aminoglycosides
As said before, these inhibit formation of the

initiation complex and cause misreading of the mRNA. Target AEROBIC GRAM-NEGATIVE RODS.

Aminoglycosides
As said before, these inhibit formation of the

initiation complex and cause misreading of the mRNA. Target AEROBIC GRAM-NEGATIVE RODS. Therefore require O2 for uptake.

Aminoglycosides
As said before, these inhibit formation of the

initiation complex and cause misreading of the mRNA. Target AEROBIC GRAM-NEGATIVE RODS. Therefore require O2 for uptake. Synergistic with beta-lactams (beta-lactams permit the aminoglycoside to enter the cell)

Aminoglycosides
As said before, these inhibit formation of the

initiation complex and cause misreading of the mRNA. Target AEROBIC GRAM-NEGATIVE RODS. Therefore require O2 for uptake. Synergistic with beta-lactams (beta-lactams permit the aminoglycoside to enter the cell) Neomycin is used for bowel surgery

Aminoglycosides
Nephrotoxic with cephalosporins and ototoxic with

loop diuretics. I remembered this through ALOe. I thought of aloe vera Aminoglycosides, Loops, Ototoxicity; so that leaves nephrotoxicity for cephs + aminos.

Aminoglycosides
Nephrotoxic with cephalosporins and ototoxic with

loop diuretics. I remembered this through ALOe. I thought of aloe vera Aminoglycosides, Loops, Ototoxicity; so that leaves nephrotoxicity for cephs + aminos. FA also mentions that its a teratogen. USMLE wants you to know that aminoglycosides can cause congenital deafness. FA (in the embryo chapter) mentions CNVIII toxicity for aminoglycosides.

Aminoglycosides
Resistance is via acetylation, phosphorylation,

adenylation. Sounds low-yield, but its in FA, and Ive seen it in two practice questions (perfect example of a 260+ question bc most people wont make the effort to remember that).

Aminoglycosides (beyond FA)

All of the following has rocked up in questions:

Aminoglycosides (beyond FA)

All of the following has rocked up in questions:
Can only be given PARENTERALLY

Aminoglycosides (beyond FA)

All of the following has rocked up in questions:
Can only be given PARENTERALLY Can also be given with nafcillin for MRSA endocarditis

(remember beta-lactam synergy?)

Aminoglycosides (beyond FA)

All of the following has rocked up in questions:
Can only be given PARENTERALLY Can also be given with nafcillin for MRSA endocarditis

(remember beta-lactam synergy?) Neomycin for bowel surgery, right? Well it also kills urease(+) organisms decreased NH3 production decreased NH3 absorption = Tx for hyperammonaemia. FA mentions lactulose for NH3 and the gut, but they dont mention neomycin.

Aminoglycosides (beyond FA)

Contraindicated in myasthenia gravis because it can

cause excessive neuromuscular blockade when used with curariform drugs (curare is a neuromuscular blocking agent at nAChR).

Aminoglycosides (beyond FA)

Contraindicated in myasthenia gravis because it can

cause excessive neuromuscular blockade when used with curariform drugs (curare is a neuromuscular blocking agent at nAChR). Any kidney damage via aminoglycosides, in terms of USMLE questions, is ATN. They might try to trick you with tubulointerstitial nephritis, but its always ATN.

Aminoglycosides (beyond FA)

Contraindicated in myasthenia gravis because it can

cause excessive neuromuscular blockade when used with curariform drugs (curare is a neuromuscular blocking agent at nAChR). Any kidney damage via aminoglycosides, in terms of USMLE questions, is ATN. They might try to trick you with tubulointerstitial nephritis, but its always ATN. Ampicillin + gentamycin = 1st-line Tx for severe pyelonephritis.

Tetracyclines
Like aminoglycosides, 30S, and as said before, they

prevent aminoacyl-tRNA binding to the A-site.

Tetracyclines
Like aminoglycosides, 30S, and as said before, they

prevent aminoacyl-tRNA binding to the A-site. Doxycycline is eliminated through feces. Good in pts with renal insufficiency. Theyll ask you that. They also want you to know that its ideal Tx for Lyme disease.

Tetracyclines
Like aminoglycosides, 30S, and as said before, they

prevent aminoacyl-tRNA binding to the A-site. Doxycycline is eliminated through feces. Good in pts with renal insufficiency. Theyll ask you that. They also want you to know that its ideal Tx for Lyme disease. Cant take tetracyclines with antacids. The same thing goes for fluoroquinolones. Ive seen both in questions.

Tetracyclines
Causes teeth discoloration IN CHILDREN and

photosensitivity IN ADULTS. guy gets sunburn while on vacation, which drug.. or girl gets sunburn/blisters while being treated for STD (gotta know Chlamydia)

Tetracyclines
Causes teeth discoloration IN CHILDREN and

photosensitivity IN ADULTS. guy gets sunburn while on vacation, which drug.. or girl gets sunburn/blisters while being treated for STD (gotta know Chlamydia) VACUUM THe BedRoom vibrio, acne, chlamydia, ureaplasma urealyticum, mycoplasma, tularaemia, H. pylori, Borellia (Lyme disease), Rickettsia rickettsii.

Tetracyclines
Causes teeth discoloration IN CHILDREN and

photosensitivity IN ADULTS. guy gets sunburn while on vacation, which drug.. or girl gets sunburn/blisters while being treated for STD (gotta know Chlamydia) VACUUM THe BedRoom vibrio, acne, chlamydia, ureaplasma urealyticum, mycoplasma, tularaemia, H. pylori, Borellia (Lyme disease), Rickettsia rickettsii. Resistance mechanism is increased efflux or reduced influx. Contrast with aminoglycosides.

Tetracyclines (beyond FA)

MINOCYCLINE, like doxycycline, is also fecally

eliminated. Minocycline, like HIPP, can cause SLElike syndrome.

Tetracyclines (beyond FA)

MINOCYCLINE, like doxycycline, is also fecally

eliminated. Minocycline, like HIPP, can cause SLElike syndrome. Tetracyclines can cause pill-induced esophagitis.

Tetracyclines (beyond FA)

MINOCYCLINE, like doxycycline, is also fecally

eliminated. Minocycline, like HIPP, can cause SLElike syndrome. Tetracyclines can cause pill-induced esophagitis. Not good for meningitis because they dont cross the BBB (FA vaguely mentions limited CNS penetration)

Tetracyclines (beyond FA)

MINOCYCLINE, like doxycycline, is also fecally

eliminated. Minocycline, like HIPP, can cause SLElike syndrome. Tetracyclines can cause pill-induced esophagitis. Not good for meningitis because they dont cross the BBB (FA vaguely mentions limited CNS penetration) Demeclocycline causes diabetes insipidus (FA says it probably in the renal chapter but not in the micro chapter, although its ridiculously high-yield)

Macrolides
Once again, they block translocation via binding to

the 23S rRNA. (elaborate)

Macrolides
Once again, they block translocation via binding to

the 23S rRNA. (elaborate) Ultra-high-yield: Tx for ATYPICAL PNEUMONIAS.

Macrolides
Once again, they block translocation via binding to

the 23S rRNA. (elaborate) Ultra-high-yield: Tx for ATYPICAL PNEUMONIAS. Prolongs the QT-interval (FA says erythromycin)

Macrolides
Once again, they block translocation via binding to

the 23S rRNA. (elaborate) Ultra-high-yield: Tx for ATYPICAL PNEUMONIAS. Prolongs the QT-interval (FA says erythromycin) DOC for pneumonia in penicillin-allergenic pts keep in mind we have aztreonam, so why use a macrolide?

Macrolides
Once again, they block translocation via binding to

the 23S rRNA. (elaborate) Ultra-high-yield: Tx for ATYPICAL PNEUMONIAS. Prolongs the QT-interval (FA says erythromycin) DOC for pneumonia in penicillin-allergenic pts keep in mind we have aztreonam, so why use a macrolide? GI-discomfort, cholestatic hepatitis, eosinophilia/rash; inhibits P-450. bleeding diathesis in heart valve pt.

Macrolides
Resistance is alteration of the 23S rRNA binding site

Macrolides (beyond FA)

The reason erythromycin causes acute cholestatic

hepatitis is because its the only macrolide eliminated through bile (USMLE would simply ask you for the mechanism of elimination, but if you remember it causes cholestatic hepatitis, it would be easy).

Macrolides (beyond FA)

The reason erythromycin causes acute cholestatic

hepatitis is because its the only macrolide eliminated through bile (USMLE would simply ask you for the mechanism of elimination, but if you remember it causes cholestatic hepatitis, it would be easy). Because erythromycin is eliminated through bile, it also is the only macrolide that doesnt require dosage adjustment in renal failure pts.

Macrolides (beyond FA)

The reason erythromycin causes acute cholestatic

hepatitis is because its the only macrolide eliminated through bile (USMLE would simply ask you for the mechanism of elimination, but if you remember it causes cholestatic hepatitis, it would be easy). Because erythromycin is eliminated through bile, it also is the only macrolide that doesnt require dosage adjustment in renal failure pts. Macrolides are DOC if pt also has post-operative ileus because they are agonists at the motilin receptor (MMCs).

Macrolides (beyond FA)

Ive seen in a practice question where they wanted to

know which drug was most injurious to myocytes. Macrolides was the answer (although protein synthesis inhibitors in general would also be correct). The rationale is that although eukaryotes have 40S/60S (80S) ribosomes, because mitochondria are derived from prokaryotes, they have 30S/50S (70S) ribosomal systems, so muscle, which is heavily mitochondriadependent, can theoretically be injured by protein synthesis inhibitors.

Macrolides vs tetracyclines
I had seen in a practice question that a pt had an

atypical pneumonia and then developed severe sunburn on vacation. They had asked which drug was used. Youve gotta know that atypical pneumonias are classically mycoplasma, chlamydia or legionella. And youve gotta remember that despite macrolides serving as the 1st-line Tx for atypical pneumonias, tetracyclines treat chlamydia, so in this case, the guy had had an atypical chlamydial pneumonia treated with a tetracycline, and he developed photosensitivity.

Chloramphenicol
Blocks peptide bond formation, but if you see it in a

question, they want you to know that it knocks out peptidyl transferase.

Chloramphenicol
Blocks peptide bond formation, but if you see it in a

question, they want you to know that it knocks out peptidyl transferase. In turn, resistance is via a plasmid-encoded acetyltransferase that inactivates the drug (FA says this, but on the USMLE, theyll reword it as bacterial enzymatic acetylation to see if you actually know what acetyltransferase means).

Chloramphenicol
Blocks peptide bond formation, but if you see it in a

question, they want you to know that it knocks out peptidyl transferase. In turn, resistance is via a plasmid-encoded acetyltransferase that inactivates the drug (FA says this, but on the USMLE, theyll reword it as bacterial enzymatic acetylation to see if you actually know what acetyltransferase means). Causes aplastic anaemia and grey baby syndrome

Chloramphenicol
Used commonly to treat meningitis in the third-

world because of its cheap cost

Chloramphenicol (beyond FA)

Kaplan liked chloramphenicol as Tx for Lyme disease

in early pregnancy > doxycycline. However, if near term, doxy is > chloramphenicol. Both drugs carry fetal risks.

Chloramphenicol (beyond FA)

Kaplan liked chloramphenicol as Tx for Lyme disease

in early pregnancy > doxycycline. However, if near term, doxy is > chloramphenicol. Both drugs carry fetal risks. USMLE will ask you to pick out the most lipophilic drug of the bunch. Chloramphenicol, bc its great for meningitis due to its BBB penetration, is heavily lipophilic.

Clindamycin
Blocks peptide bond formation

Clindamycin
Blocks peptide bond formation
Anaerobic infections ABOVE the diaphragm

(metronidazole is below)

Clindamycin
Blocks peptide bond formation
Anaerobic infections ABOVE the diaphragm

(metronidazole is below) Good for lung abscesses or aspiration pneumonia

Clindamycin
Blocks peptide bond formation
Anaerobic infections ABOVE the diaphragm

(metronidazole is below) Good for lung abscesses or aspiration pneumonia Causes pseudomembranous colitis bc it allows for C. difficile overgrowth

Clindamycin
Only real thing Ive found outside FA for this drug is

that it can also treat MRSA. Or theyll make pseudomembranous colitis apparent then ask you for the AB-toxin test or MOA of C. difficile-mediated necrosis.

Linezolid
For this drug, you only need to remember two things:

Linezolid
For this drug, you only need to remember two things:
1) it acts on the 50S subunit (binds to 23S of 50S

subunit - same as macrolides/clindamycin)

Linezolid
For this drug, you only need to remember two things:
1) it acts on the 50S subunit (binds to 23S of 50S

subunit - same as macrolides/clindamycin) 2) Side-effects are HOT:

Linezolid
For this drug, you only need to remember two things:
1) it acts on the 50S subunit (binds to 23S of 50S

subunit - same as macrolides/clindamycin) 2) Side-effects are HOT: H = High risk of serotonin syndrome

Linezolid
For this drug, you only need to remember two things:
1) it acts on the 50S subunit (binds to 23S of 50S

subunit - same as macrolides/clindamycin) 2) Side-effects are HOT: H = High risk of serotonin syndrome O = optic neuritis

Linezolid
For this drug, you only need to remember two things:
1) it acts on the 50S subunit (binds to 23S of 50S

subunit - same as macrolides/clindamycin) 2) Side-effects are HOT: H = High risk of serotonin syndrome O = optic neuritis T = thrombocytopenia

Linezolid
For this drug, you only need to remember two things:
1) it acts on the 50S subunit (binds to 23S of 50S

subunit - same as macrolides/clindamycin) 2) Side-effects are HOT: H = High risk of serotonin syndrome O = optic neuritis T = thrombocytopenia

Linezolid
I had only ever encountered one question on this drug,

and it was a vignette of someone with serotonin syndrome (flushing, diarrhea, sweating, hyperthermia, agitation, etc.) and blurry vision, and then they wanted to know the drug that caused the Sx.

Sulfonamides vs trimethoprim

Sulfonamides vs trimethoprim
Sulfadiazine + pyrimethamine used to Tx

toxoplasmosis are analogous. So if they ask you for the MOA of pyrimethamine, you know its like trimethoprim.

Sulfonamides
Dihydropteroate synthase inhibitors (PABA analogues)

Sulfonamides
Dihydropteroate synthase inhibitors (PABA analogues)
Simple UTIs

Sulfonamides
Dihydropteroate synthase inhibitors (PABA analogues)
Simple UTIs Can treat Nocardia (SNAP)

Sulfonamides
Dihydropteroate synthase inhibitors (PABA analogues)
Simple UTIs Can treat Nocardia (SNAP)

Can treat Chlamydia, as per FA, but Ive never seen this

in a practice question

Sulfonamides
Dihydropteroate synthase inhibitors (PABA analogues)
Simple UTIs Can treat Nocardia (SNAP)

Can treat Chlamydia, as per FA, but Ive never seen this

in a practice question Can cause hypersensitivity rxns (tubulointersititial nephritis; in contrast to aminoglycosides ATN)

Sulfonamides
Dihydropteroate synthase inhibitors (PABA analogues)
Simple UTIs Can treat Nocardia (SNAP)

Can treat Chlamydia, as per FA, but Ive never seen this

in a practice question Can cause hypersensitivity rxns (tubulointersititial nephritis; in contrast to aminoglycosides ATN) Haemolysis in G6PD-deficiency (ISPAIN)

Sulfonamides
Dihydropteroate synthase inhibitors (PABA analogues)
Simple UTIs Can treat Nocardia (SNAP)

Can treat Chlamydia, as per FA, but Ive never seen this

in a practice question Can cause hypersensitivity rxns (tubulointersititial nephritis; in contrast to aminoglycosides ATN) Haemolysis in G6PD-deficiency (ISPAIN) Displaces drugs from albumin kernicterus

Sulfonamides (beyond FA)

So if a pregnant woman takes a drug and the baby has

jaundice kernicterus sulfonamide.

Sulfonamides (beyond FA)

So if a pregnant woman takes a drug and the baby has

jaundice kernicterus sulfonamide. Because the bilirubin is displaced from albumin, it can cross the BBB kernicterus

Trimethoprim
Inhibits dihydrofolate reductase

Trimethoprim
Inhibits dihydrofolate reductase
Once again, sulfonamides and trimethoprim are both

BACTERISTATIC, but if combined (TMP-SMX), they are BACTERICIDAL.

Trimethoprim
Inhibits dihydrofolate reductase
Once again, sulfonamides and trimethoprim are both

BACTERISTATIC, but if combined (TMP-SMX), they are BACTERICIDAL. Highest-yield for TMP-SMX is Pneumocystis jiroveci pneumonia. If high-yield were diarrhea, it would be explosive here.

Trimethoprim
Inhibits dihydrofolate reductase
Once again, sulfonamides and trimethoprim are both

BACTERISTATIC, but if combined (TMP-SMX), they are BACTERICIDAL. Highest-yield for TMP-SMX is Pneumocystis jiroveci pneumonia. If high-yield were diarrhea, it would be explosive here. Can Tx SALMONELLA and SHIGELLA

Trimethoprim
Inhibits dihydrofolate reductase
Once again, sulfonamides and trimethoprim are both

BACTERISTATIC, but if combined (TMP-SMX), they are BACTERICIDAL. Highest-yield for TMP-SMX is Pneumocystis jiroveci pneumonia. If high-yield were diarrhea, it would be explosive here. Can Tx SALMONELLA and SHIGELLA May cause megaloblastic anaemia or (or any penia).

Trimethoprim
May alleviate TMP-induced megaloblastic anaemia

with leucovorin rescue folinic acid

Trimethoprim
May alleviate TMP-induced megaloblastic anaemia

with leucovorin rescue folinic acid As stated before, remember the pyrimethamine for toxoplasmosis has same MOA

Fluoroquinolones
-floxacin drugs; inhibit bacterial topoisomerase II

(aka DNA gyrase) very very high-yield

Fluoroquinolones
-floxacin drugs; inhibit bacterial topoisomerase II

(aka DNA gyrase) very very high-yield Like tetracyclines, cannot be taken with antacids

Fluoroquinolones
-floxacin drugs; inhibit bacterial topoisomerase II

(aka DNA gyrase) very very high-yield Like tetracyclines, cannot be taken with antacids Can treat a range of organisms (mostly gram(-) rods)

Fluoroquinolones
-floxacin drugs; inhibit bacterial topoisomerase II

(aka DNA gyrase) very very high-yield Like tetracyclines, cannot be taken with antacids Can treat a range of organisms (mostly gram(-) rods) Can cause cartilage damage or tendon rupture

Fluoroquinolones
-floxacin drugs; inhibit bacterial topoisomerase II

(aka DNA gyrase) very very high-yield Like tetracyclines, cannot be taken with antacids Can treat a range of organisms (mostly gram(-) rods) Can cause cartilage damage or tendon rupture Leg cramps + myalgias in kids (as per FA, but Ive never seen this in a practice question)

Fluoroquinolones
Bacterial resistance is via chromosome-mediated

mutation in DNA gyrase. FA mentions this, but its not minutiae. Ive seen in a couple questions where they want you to know that bacterial resistance is ALMOST ALWAYS plasmid-mediated, but fluoros are the exception because theyre chromosomal.

Fluoroquinolones (beyond FA)

First-line Tx for diarrhea due to Shigella + travelers

diarrhea

Fluoroquinolones (beyond FA)

First-line Tx for diarrhea due to Shigella + travelers

diarrhea Ciprofloxacin is DOC for UTIs in pts with sulfa allergy. If pt has sulfa allergy and has GERD nitrofurantoin

Fluoroquinolones (beyond FA)

First-line Tx for diarrhea due to Shigella + travelers

diarrhea Ciprofloxacin is DOC for UTIs in pts with sulfa allergy. If pt has sulfa allergy and has GERD nitrofurantoin Kaplan liked that H. influenzae can also be treated by fluoros.

Fluoroquinolones (beyond FA)

First-line Tx for diarrhea due to Shigella + travelers

diarrhea Ciprofloxacin is DOC for UTIs in pts with sulfa allergy. If pt has sulfa allergy and has GERD nitrofurantoin Kaplan liked that H. influenzae can also be treated by fluoros. GOOD ORAL BIOAVAILABILITY. That rocked up on an NBME exam (Gd knows why), which means it was once (or still is) floating around on the real deal.

Fluoroquinolones (beyond FA)

Gatifloxacin causes dysglycaemia (rocked up in GT)

Fluoroquinolones (beyond FA)

Gatifloxacin causes dysglycaemia (rocked up in GT)
Moxifloxacin causes heart conduction abnormalities

Fluoroquinolones (beyond FA)

Gatifloxacin causes dysglycaemia (rocked up in GT)
Moxifloxacin causes heart conduction abnormalities USMLE might try to re-word the MOA as that which

affects the DNA nicking process

Metronidazole
Forms toxic metabolites that damage DNA

Metronidazole
Forms toxic metabolites that damage DNA
GET GAP Tx for Giardia, Entamoeba, Trichomonas,

Gardnerella vaginalis, Anaerobes, H. pylori

Metronidazole
Forms toxic metabolites that damage DNA
GET GAP Tx for Giardia, Entamoeba, Trichomonas,

Gardnerella vaginalis, Anaerobes, H. pylori USMLE will literally give you a classic vignette / presentation of one of the above organisms, then ask for the Tx. They might tell you clue cells, for instance, then ask for the Tx, or theyll tell you a guy went swimming in a lake and now has steatorrhoea, then ask for the Tx, etc.

Metronidazole
Disulfiram-like rxn with EtOH headache /

metallic taste in the mouth

Metronidazole
Disulfiram-like rxn with EtOH headache /

metallic taste in the mouth Once again, clindamycin = anaerobes ABOVE diaphragm; metronidazole = BELOW

Metronidazole (beyond FA)

FA doesnt say it for metro at the end of the micro

chapter, although it does under C. difficile, but metronidazole is a TREATMENT FOR pseudomembranous colitis. Vancomycin and metro are both Txs for it, but if a question has both as answer choices, metro is always correct > vancomycin because of the scare of vancomycin-resistant organisms. Another thing: vanco is always given IV (poor oral bioavailability), but for pseudomembranous colitis, ITS GIVEN ORALLY (makes sense bc it stays confined to the gut).

Metronidazole (beyond FA)

Ive also seen it in a practice question: USMLE likes

metronidazole CREAM as Tx for acne rosacea. But erythromycin CREAM is Tx for acne vulgaris.

Polymyxins
USMLE for some reason likes you to know the

structures/MOA of these drugs really well. Know that they have a long hydrophobic tail and that they are cationic and basic. They act as detergents on the cell membrane and DISRUPT ITS OSMOTIC PROPERTIES.

Polymyxins
USMLE for some reason likes you to know the

structures/MOA of these drugs really well. Know that they have a long hydrophobic tail and that they are cationic and basic. They act as detergents on the cell membrane and DISRUPT ITS OSMOTIC PROPERTIES. Theyre used only when a pt is resistant to practically all other Txs very high neuro- and nephrotoxicity

Polymyxins
Youll find them in the THAYER-MARTIN medium

used to culture N. gonorrhea. TM medium = VPN client = vancomycin (hits gram+), Polymyxin = colistin (hits other gram-), nystatin (hits fungi)

Daptomycin
Dont be fooled by the name. Its not an

aminoglycoside, nor is it a macrolide.

Daptomycin
Dont be fooled by the name. Its not an

aminoglycoside, nor is it a macrolide. Youve gotta know that it creates membrane channels that result in depolarization and **disruption of the membrane potential.**

Daptomycin
Dont be fooled by the name. Its not an

aminoglycoside, nor is it a macrolide. Youve gotta know that it creates membrane channels that result in depolarization and **disruption of the membrane potential.** Its notable side-effect is myopathy with increased CPK (so contraindicated with statins/fibrates)

Daptomycin
Dont be fooled by the name. Its not an

aminoglycoside, nor is it a macrolide. Youve gotta know that it creates membrane channels that result in depolarization and **disruption of the membrane potential.** Its notable side-effect is myopathy with increased CPK (so contraindicated with statins/fibrates) It is only effective against gram(+)s.

Daptomycin
Dont be fooled by the name. Its not an

aminoglycoside, nor is it a macrolide. Youve gotta know that it creates membrane channels that result in depolarization and **disruption of the membrane potential.** Its notable side-effect is myopathy with increased CPK (so contraindicated with statins/fibrates) It is only effective against gram(+)s. Cannot be used for pneumonia bc pulmonary surfactant inhibits it.

Anti-TB drugs

Anti-TB drugs
Prophylaxis = isoniazid. High-yield.

Anti-TB drugs
Prophylaxis = isoniazid. High-yield.
Tx = RIPE = rifampin, isoniazid, pyrazinamide,

ethambutol

Anti-TB drugs
Prophylaxis = isoniazid. High-yield.
Tx = RIPE = rifampin, isoniazid, pyrazinamide,

ethambutol All are HEPATOTOXIC, except for ethambutol, which causes VISUAL CHANGES.

Rifampin
Inhibits DNA-dependent RNA-polymerase. USMLE

really likes that. And theyll really nail it. Theyll literally list RNA-dependent DNA-polymerase and other types of similar polymerases to trick you, but you need to remember that rifampin inhibits DNAdependent RNA-polymerase. That means it prevents RNA synthesis. The way I remembered this was RDR = rifampin-DNA-RNA. Whatever works for you. Just find a way to remember it.

Rifampin
FA says that rifampin delays resistance to dapsone

when used for leprosy. Ive never actually seen a question on this though.

Rifampin
FA says that rifampin delays resistance to dapsone

when used for leprosy. Ive never actually seen a question on this though. As said prior, isoniazid, NOT rifampin, is the prophylaxis for TB. But you need to remember that rifampin is the prophylaxis for N. meningitidis and H. influenzae type-B.

Rifampin
FA says that rifampin delays resistance to dapsone

when used for leprosy. Ive never actually seen a question on this though. As said prior, isoniazid, NOT rifampin, is the prophylaxis for TB. But you need to remember that rifampin is the prophylaxis for N. meningitidis and H. influenzae type-B. Ill summarize that last point bc its so important:

 TB prophylaxis = isoniazid
TB Tx = rifampin, isoniazid, pyrazinamide, ethambutol N. meningitidis / H. influenzae prophylaxis =

rifampin N. meningitidis Tx = ceftriaxone (FA says ceftriaxone or penicillin G, but in terms of USMLE questions, Ive only ever seen ceftriaxone)

Rifampin (contd)
Upregulates P-450 (very high-yield)

Rifampin (contd)
Upregulates P-450 (very high-yield)
Can cause orange tears/sweat/urine (high-yield);

harmless side-effect, but pt will be concerned

Rifampin (beyond FA)

Since rifampin upregulates P-450, you dont want to

give it to HIV pts on protease inhibitors bc youll increase the rate of the latters degradation, so you instead give rifabutin. USMLE will list both as Txs for TB in the same question, but the answer is rifabutin if the pt is HIV(+) bc you need to assume he/she is on HAART.

Rifampin (beyond FA)

Since rifampin upregulates P-450, you dont want to

give it to HIV pts on protease inhibitors bc youll increase the rate of the latters degradation, so you instead give rifabutin. USMLE will list both as Txs for TB in the same question, but the answer is rifabutin if the pt is HIV(+) bc you need to assume he/she is on HAART. USMLE wants to know WHY rifampin is prophylaxis for N. meningitidis.

Rifampin (beyond FA)

Since rifampin upregulates P-450, you dont want to

give it to HIV pts on protease inhibitors bc youll increase the rate of the latters degradation, so you instead give rifabutin. USMLE will list both as Txs for TB in the same question, but the answer is rifabutin if the pt is HIV(+) bc you need to assume he/she is on HAART. USMLE wants to know WHY rifampin is prophylaxis for N. meningitidis. ..it penetrates the respiratory tract wall and prevents meningococcal pharyngeal colonization.

Rifampin (beyond FA)

Rifampin binds the beta-subunit of DNA-dependent

RNA-polymerase. Retarded, but it showed up in a question (lord knows why). This is an example of how everyone needs a little luck on the real deal.

Rifampin (beyond FA)

Rifampin binds the beta-subunit of DNA-dependent

RNA-polymerase. Retarded, but it showed up in a question (lord knows why). This is an example of how everyone needs a little luck on the real deal. USMLE might tell you the pts contact lenses were rusty colored as an indirect reference to the orange tears a little trickier

Rifampin (beyond FA)

Rifampin binds the beta-subunit of DNA-dependent

RNA-polymerase. Retarded, but it showed up in a question (lord knows why). This is an example of how everyone needs a little luck on the real deal. USMLE might tell you the pts contact lenses were rusty colored as an indirect reference to the orange tears a little trickier Why is isoniazid used over rifampin for TB prophylaxis?

Rifampin (beyond FA)

Rifampin binds the beta-subunit of DNA-dependent

RNA-polymerase. Retarded, but it showed up in a question (lord knows why). This is an example of how everyone needs a little luck on the real deal. USMLE might tell you the pts contact lenses were rusty colored as an indirect reference to the orange tears a little trickier Why is isoniazid used over rifampin for TB prophylaxis? ..bc rifampin is way more hepatotoxic.

Isoniazid (INH)
Decreases synthesis of mycolic acids (high-yield)

Isoniazid (INH)
Decreases synthesis of mycolic acids (high-yield)
KatG (catalase-peroxidase) needed to convert INH

to active metabolite

Isoniazid (INH)
Decreases synthesis of mycolic acids (high-yield)
KatG (catalase-peroxidase) needed to convert INH

to active metabolite Although hepatotoxic, USMLE loves that INH is a common cause of vitamin B6 deficiency. Quite HY.

Isoniazid (INH)
Decreases synthesis of mycolic acids (high-yield)
KatG (catalase-peroxidase) needed to convert INH

to active metabolite Although hepatotoxic, USMLE loves that INH is a common cause of vitamin B6 deficiency. Quite HY. Theyll tell you a person with TB was treated and now has paresthesias (or sometimes seizures) B6 def.

Isoniazid (beyond FA)

KatG needed to convert INH to active metabolite does

so via acyl-carrier and ketoacyl-carrier protein synthesis. So KatG is a catalase-peroxidase enzyme that carries out acyl-carrier and ketoacyl-carrier protein synthesis.

Isoniazid (beyond FA)

KatG needed to convert INH to active metabolite does

so via acyl-carrier and ketoacyl-carrier protein synthesis. So KatG is a catalase-peroxidase enzyme that carries out acyl-carrier and ketoacyl-carrier protein synthesis. USMLE loves to ask about metabolism of INH. If they show you a bimodal curve of INH metabolism, youve gotta know that people demonstrate phenotypic variation regarding fast vs slow acetylation.

Isoniazid (beyond FA)

Ive seen a practice question where the hepatotoxicity

of INH was presented as fever, anorexia and nausea, WITHOUT jaundice. Effects can be acute. If they throw neurotoxicity at you, it will be the paresthesias. If its hepatotoxicity, youll get the former Sx (just think, in paracetamol poisoning, does jaundice develop?)

Isoniazid (beyond FA)

USMLE wants you to know WHY isoniazid causes B6

deficiency.

Isoniazid (beyond FA)

USMLE wants you to know WHY isoniazid causes B6

deficiency. Its bc INH inhibits pyridoxal kinase, so B6 cannot get activated (normally pyridoxine pyridoxal phosphate).

Isoniazid (beyond FA)

USMLE wants you to know WHY isoniazid causes B6

deficiency. Its bc INH inhibits pyridoxal kinase, so B6 cannot get activated (normally pyridoxine pyridoxal phosphate). INH can cause D/NE/E deficiency (secondary to the B6 deficiency)

Isoniazid (beyond FA)

USMLE wants you to know WHY isoniazid causes B6

deficiency. Its bc INH inhibits pyridoxal kinase, so B6 cannot get activated (normally pyridoxine pyridoxal phosphate). INH can cause D/NE/E deficiency (secondary to the B6 deficiency) Seizures secondary to B6 deficiency and INH occur bc of decreased GABA (bc you need B6 to decarboxylate glutamate to GABA!).

Pyrazinamide
Knocks out mycobacterial fatty acid synthase I

Pyrazinamide
Knocks out mycobacterial fatty acid synthase I
Effective in the acidic pH of phagolysosomes

Pyrazinamide
Knocks out mycobacterial fatty acid synthase I
Effective in the acidic pH of phagolysosomes Although it causes hepatotoxicity, it also causes gout

(hyperuricaemia). On my real deal, I was asked the lower-yield side-effect of a drug (hint hint).

Pyrazinamide (beyond FA)

Like INH, pyrazinamide requires activation by TB

Pyrazinamide (beyond FA)

Like INH, pyrazinamide requires activation by TB
FA mentions that pyrazinamide is most effective in the

acidic pH of phagolysosomes, but what this means is that pyrazinamide is the most effective intracellular TB medication thats how theyll ask it. The other drugs still penetrate the cell, but pyrazinamide is simply the best at killing TB that have already been engulfed by macrophages.

Ethambutol
Decreases carbohydrate polymerization of the

mycobacterial cell wall by blocking arabinosyltransferase.

Ethambutol
Decreases carbohydrate polymerization of the

mycobacterial cell wall by blocking arabinosyltransferase. So they want you to know that ethambutol knocks out carbohydrate synthesis but that pyrazinamide knocks out fatty acid synthesis. Isoniazid hits mycolic acid synthesis directly, and rifampin inhibits nucleic acid synthesis.

Ethambutol
Causes optic neuropathy. FA says red-green color

blindness for ethambutol, but Ive seen it in questions as decreased visual acuity and central scotoma.

Ethambutol
Causes optic neuropathy. FA says red-green color

blindness for ethambutol, but Ive seen it in questions as decreased visual acuity and central scotoma. Although, of RIPE, RIP are hepatotoxic, ethambutol causes VISUAL PROBLEMS, so if they list hepatotoxicity and central scotoma as two answers, the latter is right, even though FA only says red-green color blindness.

Ethambutol
Causes optic neuropathy. FA says red-green color

blindness for ethambutol, but Ive seen it in questions as decreased visual acuity and central scotoma. Although, of RIPE, RIP are hepatotoxic, ethambutol causes VISUAL PROBLEMS, so if they list hepatotoxicity and central scotoma as two answers, the latter is right, even though FA only says red-green color blindness. Tangential: sildenafil instead causes blue-green color blindness.

TB drugs in general
RIPES can also be used instead of RIPE. If they ask

about an aminoglycoside, streptomycin has shown some use against TB.

TB drugs in general
RIPES can also be used instead of RIPE. If they ask

about an aminoglycoside, streptomycin has shown some use against TB. Ive seen in a practice question cycloserine as general TB Tx. It is a broad-spectrum TB drug and can cause CNS effects/seizures.