Beruflich Dokumente
Kultur Dokumente
Riley Ennis is a sophomore at Dartmouth College and founder of Immudicon. The company began from an idea Riley had to model the horseshoe crab immune system in order to teach the body to recognize and destroy the cancer.
Treat
DIAGNOSE
Pipeline overview
Treat (MacroVax)
Macrovax
Mannose Binding Lectin
PLGA
OR
Key Advantages
Mannose Binding Lectin Serves as the Basis of the Human Immune System
Current Research:
Research has been conducted for three years at Georgetown (Dr. Anita Sidhu and Dr. Chris Albanese), Childrens National Medical Center (Dr. Anthony Sandler and Dr. Larry Mahan), and the Dartmouth Hitchcock Medical Center (Dr. Barjor Gimi). The next couple of slides outline some of the data collected.
Epifluorescent image of MacroVax- treated dendritic cells and macrophages. The vaccine complex is shown here to bind to and be phagocytosed by these antigen presenting cells. Actin (Red), Nuclei (Blue), Vaccine Complex (Green). 1200X magnification.
Confocal Image of Human Dendritic Cell Proves MacroVax Enters APCs Directly
MERGE: Confocal image of MacroVax-treated immature human dendritic cells. Actin (Red), Nuclei (Blue), Conjugated Nanoparticles (Green).
Mannose Binding Lectin Blocking Antibody Decreased Phagocytosis of MacroVax Indicated by Lower Levels of Intracellular GFP
10 9 8 7 6 5 4 3 2 1 0
0 10 20 30 40 50 Concentration of Blocking Antibody (ug) 60 70
Mouse macrophages were treated with a Mannose Binding Lectin Antibody (Genetex) and then treated with GFP-tagged MacroVax to study dose-response of MacroVax with respect to phagocytosis. Using a GFP Quantification Assay (BioVision) intracellular GFP
Epifluorescent image of vaccine treated dendritic cells. The MacroVax complex is shown here to bind to and be phagocytosed by these antigen presenting cells. Actin (Red), Nuclei (Blue), Vaccine Complex (Green). 1200X magnification, scale bar = 50 um.
Vaccine-Treated
Flow cytometry was performed on macrophages treated with either PLGA (vehicle) particles or the vaccine complex for three hours. The two markers of interest used were CD11b (X axis: macrophage marker) and CD86 (Y axis: activation marker).
MacroVax Upregulates Expression of Activation Cytokines and Downregulates Expression of Deactivation Cytokines in Human Dendritic Cells
In human dendritic cells the expression or transcription levels of certain chemical signals called cytokines was studied. Dendritic cells were either untreated or vaccine-treated for three hours and then subject to mRNA extraction and RT-PCR to study DNA activities posttreatment. *=P <0.001, Students T-test ** = P <0.01, Students T-test
MacroVax Induces Secretion of Activation Cytokine TNF- and Depresses Secretion of Deactivation Cytokine IL-10
IL-10 Levels in Vaccine Treated Macrophages
2000 1800 450 400
1600
1400 1200 1000 800 600 400 200 0 1 2 3 4
350
300
Pg/ml
Pg/ml
Legend: 1- PLGA + Lysate 2- PLGA + Immunostimulatory Agents + Lysate 3- Microbial PLGA + CpG + GM-CSF + Lysate 4- MacroVax
T Cell Proliferation Assay and IFN-y MacroVax Induces Generation of Tumor-Specific T Lymphocytes Staining
Prime Immune System With Vaccine Treatment (Day 1) Boost Immune System With Vaccine Treatment (Day 14) Collect Mouse Spleens and Isolate Splenocytes (Day 21)
MacroVax Induces Generation of Tumor-Specific T Cell Proliferation Assay and IFN-y T Lymphocytes Staining
Survivin
Mouse T lymphocytes collected from PLGA/lysate-treated mice (left) and vaccine-treated mice (right) spleens (3 weeks of treatment) were exposed to tumor specific antigens and analyzed for clonal expansion via a cell proliferation assay.
Untreated Lymphocytes
DIAGNOSEME
Proteins indicative of immune activity are found in your sweat and we can measure these proteins with our telemedicine platform.
8
6 4 2 0
Sample 1
Sample 2
Average
Sweat Sample
1 2
Optical Density
.0975 .0801
?
Reading 1 Reading 2
Reading 3
2.5
3.5
Reading 1
Reading 2
Reading 3
Studied the effects of chemical impurities such as deodorant on the biosensor readings
10
8 6 4 2 0 Control Sunscreen Lotion Impurities Perfume Deodorant
Control
Sunscreen
Lotion
Perfume
Deodorant
Studied the effect of sweat sample volume on the accuracy of the biosensor
0
300 250 200 150 Volume of Sample (l) 100 Average
300 l (Control)
250 l
200 l
150 l
1oo l