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DNA: PART II
LETS REVIEW MITOSIS AND also COVER PROTEIN SYNTHESIS
Threadlike chromatin = chromosomes = 46 DNA molecules and associated proteins Nondividing state = DNA molecules compacted
coiled around core particle (histone protein) zig-zagged, looped and coiled onto itself DNA copies itself to form 2 parallel sister chromatids
Preparing to divide
By 1953: x ray diffraction determined geometry of DNA molecule Nobel Prize awarded in 1962 to 3 men: Watson, Crick and Wilkins but not to Rosalind Franklin who died of cancer at 37 getting the x ray data that provided the answers.
Figure 4.4
Nucleotide Structure
nitrogenous base
Nitrogenous Bases
Segment of DNA
DNA Function
Code for protein synthesis Gene - sequence of DNA nucleotides that codes for one protein Genome - all the genes of one person humans have estimated 3035,000 genes other 98% of DNA noncoding
normal female has 2 X chromosomes normal male has one X and one Y chromosome
DNA Replication 1
DNA Replication 2
Law of complimentary base pairing allows building of one DNA strand based on the bases in 2nd strand Steps of replication process
DNA
replication
DNA
2
polymerase assembles new strand of DNA next to one of the old strands
DNA polymerase enzymes at work simultaneously
some cause no effect, some kill cell, turn it cancerous or cause genetic defects in future generations
Mitosis
one cell divides into 2 daughter cells with identical copies of DNA Functions of mitosis
embryonic
G-1 This phase is longest ( ave-18-24 hours) remember this phase is the phase the most normal phase. ( Normal growth and metabolism activities- Synthesizing proteins needed for DNA synthesis) S phase ( DNA replication (next longest) 8-10 hours G-2 : relatively shorter : 4-6 hours ( Replicating centrioles and synthesizing enzymes that control cell division. M phase is only 1-2 hours long.
DNA directs the synthesis of all cell proteins including enzymes that direct the synthesis of nonproteins Different cells synthesize different proteins dependent upon differing gene activation See Next Example
Figure 4.5
The process of The majority of genes are expressed as the proteins they encode. The process occurs in two steps: Transcription = DNA RNA Translation = RNA protein Taken together, they make up the "central dogma" of biology: DNA RNA protein.
Protein modified in cisterna, passed to next cisterna Last golgi cisterna releases finished product as membrane bound vesicles
secretory
migrate
vesicles
lysosomes
vesicles
Polyribosome
cluster of 10-20 ribosomes reading mRNA at one time horizontal filament - mRNA large granules - ribosomes beadlike chains projecting out - newly formed proteins
takes 20 seconds to assemble protein of 400 amino acids cell may produce > 150,000 proteins/second
The process of protein synthesis involves: 1) Synthesis of mRNA from DNA template
2) Migration of mRNA from nucleus to cytoplasm ( the ribosome) where it will wait for another type of RNA ( tRNA) 3) tRNA will bring amino acids which are complimentary to the codons on mRNA
codon
Each three consecutive mRNA bases forms a genetic code word (codon) that codes for a particular amino acid.
Transfer or T- RNA
The unpaired regions form 3 loops. Each kind of tRNA carries (at its 3 end) one of the 20 amino acids At one loop, 3 unpaired bases form an anticodon. Base pairing between the anticodon and the complementary codon on a mRNA molecule brings the correct amino acid into the growing polypeptide.
tRNA (transfer RNA) - transports specific amino acids to ribosome during protein synthesis (translation).
Anticodon - specific
sequence of 3 nucleotides; complementary to an mRNA codon.
Anticodon sequence determines the specific amino acid that binds to tRNA.
Ribosomal RNA (rRNA) There are 4 kinds. In eukaryotes, these are 18S rRNA. One of these molecules, along with some 30 different protein molecules, is used to make the small subunit of the ribosome. 28S, 5.8S, and 5S rRNA. One each of these molecules, along with some 45 different
large subunit
small subunit
Subunits are separate in the cytoplasm, but join during protein synthesis (translation).
Transcription
messenger
Translation
mRNA
code is read by ribosomal RNA as amino acids are assembled into a protein molecule transfer RNA delivers the amino acids to the ribosome
Essential function
interpret DNA code direct protein synthesis in the cytoplasm
Elongation
A UA U A U G C C C G C
How many codons are in this sequence of mRNA?
Using this chart, you can determine which amino acid the codon codes for!
Tyr or tyrosine
Notice there is one start codon AUG. Transcription begins at that codon!
Notice there are three stop codons. Transcription stops when these codons are encountered.
Although we do have proofreading mechanisms in place, sometimes mutations occur and a protein is not translated properly.
Are there possible consequences to such errors in transcription? Well, errors in transcription will lead to the wrong codon and incorrect translation of amino acid and erroneous protein SO. One disease we see as and example on this is.