Beruflich Dokumente
Kultur Dokumente
MICROORGANISMS
Part I
Acellular and Prokaryotic
Microbes
Acellular Infectious
Agents:
Viruses
Virions are complete viral particles
which are very small and simple in
structure.
Size: 10 to 300nm, or can be up to
1um in length(Ebola virus)
1940: 1st photographs of viruses after
the invention of electron microscopes
in 1930s
Acellular Infectious
Agents:Viruses
Acellular Infectious
Agents:Viruses
Viruses contain DNA or RNA
A protein coat
Some are enclosed by an envelope
Some viruses have spikes
Most viruses infect only specific types
of cells in one host
Host range is determined by specific
host attachment sites and cellular
factors
Acellular Infectious
Agents:Viruses
Five specific properties that
distinguish viruses from living cells:
2. They possess either DNA or RNA
3. They are unable to replicate on their own
4. They do not divide by binary fission,
mitosis, or meiosis
5. They lack the genes and enzymes
necessary for energy production
6. They depend on the ribosomes, enzymes,
and metabolites of the host cell for protein
and nucleic acid production.
Acellular Infectious
Agents:Viruses
Viruses are classified by the
following characteristics:
2. Type of genetic material
3. Shape of the capsid
4. Number of capsomeres
5. Size of the capsid
6. Presence or absence of an envelop
7. Type of host that it infects
8. Type of disease it produces
9. Immunologic or antigenic properties
Acellular Infectious
Agents:Viruses
Four categories of viruses based
on the type of nucleic acid they
possess:
2. Double-stranded RNA viruses
3. Single-stranded RNA viruses
4. Single-stranded DNA viruses
5. Double-stranded DNA viruses
Bacteriophages
Viruses that infect bacteria
Obligate intracellular pathogens
They may be:
4. Icosahedron: almost spherical shape
with 20 facets
5. Filamentous: long tubes formed into
helical structure
6. Complex: icosahedral heads attached
to helical tails.
Acellular Infectious
Agents:Viruses
Acellular Infectious
Agents:Viruses
Virulent bacteriophages
Cause the lytic cycle, which ends in the
destruction of the bacterial cell.
The lytic cycle has 5 steps:
3. Attachment (adsorption)
4. Penetration
5. Biosynthesis
6. Assembly
7. Release
Multiplication of Bacteriophages
(Lytic Cycle)
Attachment Phage attaches by tail fibers to
host cell
Penetration Phage lysozyme opens cell wall,
Decolorizing agent:
Alcohol-acetone Purple
Colorless
Counterstain:
Safranin Purple Red
Differential Stain: Gram
Staining
Differential Stains: Acid-fast
Stain
Cells that retain a basic
stain in the presence of
acid-alcohol are called
acid-fast.
Non–acid-fast cells
lose the basic stain
when rinsed with acid-
alcohol, and are
usually counterstained
(with a different color
basic stain) to see
them.
Negative staining is useful
for capsules.
Heat is required to drive a