Beruflich Dokumente
Kultur Dokumente
FORENSIC AND MEDICOLEGAL DEPARTMENT FACULTY OF MEDICINE DIPONEGORO UNIVERSITY RSUP DR. KARIADI SEMARANG Period 23 September 19 Oktober 2013
Dika Gita Pratama Gita Soraya Untari K. Nurul Charla H. Aaron Y Tehupuring Aldo I. R. Sitanggang
DNA
Personal Identification
General Aim
Knowing the function of DNA examination for personal identification
Spesific Aim
Definition and classification of personal identification Definition, structure, and DNA testing techniques The benefits, procedure as well as the application of DNA testing in the identification of personal
FORENSIC IDENTIFICATION
is an attempt to determine the identity of a person for the benefit of forensic, which the interests of justice
PERSONAL IDENTIFICATION
One of efforts of the individual differences with other individuals or attempt to determine ownership of body parts, body fluids or other body parts are still good either been damaged or that have been destroyed even though the owner
Whole medicolegal casses suspect of muderer Suspect of rapist Child identification Missing kids
Person
Corpse
Conventional
Modern
- Molecular Forensic (DNA Testing)
Fingerprint examination Visual Method Document examination Clothes and jewelry watching Medical examination Dental examination Serology Exclusion Mutilating identification Skeletal examination
DNA is the genetic material that carries information that can be derived. DNA that usually use are c-DNA and mtDNA. The most accurate DNA sample is c-DNA
Gel Electrophoresis
Polymerase Chain Reaction (PCR) Capillary Electrophoresis (CE)
Pentanucleotide repeats
HLA-DQA1
Polymarker (PM)
Y Chromosome Markers
DNA MITOKONDRIA
mt-DNA is another choice to examine the DNA sample that cant determine by RFLP or STR Every mothers have their own mt-DNA This examination confirm a relation between mother and descent.
Y chromosome passed on from father to all his sons so that the DNA on the Y chromosome can be used to find the descendants of the male and is usually used for cases of sexual crimes This examination can detect man profile when women profile had clear with STRs
Time-consuming expensive
(+)
(-)
To identificate suspect To determine the criminality network its used as secondary evidence needs other evidence to support
There are so many technique for DNA identification. Every technique has their own advantages and disadvantages. The technique are depends on many factors, example cost, sample, etc. Then finally the result of the identification can determine the identity of a person, body parts and body fluids which the interests of court.
Definition
Techniques
RFLP is a DNA polymorphism that occurs due to variation in the length of DNA fragments after cut with a specific restriction enzyme.
This method uses a short marker, repeat pattern alleles at mikrovarian segment between 3-7 base pairs. Advantages : damaged samples, limited sample, etc. Limitation : relatively expensive and limited Possibility for DNA contamination