INTRODUCTORY AND BASIC MICROBIOLOGY

By Dr. Taufik Rahman

Introduce
Microbiology is science that studying very small organism ( diameter < 0,1 mm), cannot see with eyes, must uses specific tools. Medical microbiology studying microorganism that have relation with disease, how to preventive disease, and how to handling. Microbiology include : Bacteriology Imunology Virology Mikology Parasitology

Infection disease
Ancient ,,,, damnation of god Hipocrates, intrinsic factor and ekstrinsik

factor, Intrinsic factor, somethink in the body of human. Ekstrinsic factor, something becoming worse in the air.

Theory of Life
Generatio Spontaneous
Francesco Redi : contradicting of this theory John Needham : supporting of this theory Lazzaro Spallanzani

Theory of Life
New concept
Louis Pasteur : trapping microorganism John Tyndall : air carrying microorganism

Theory of Life
Microorganism as agent of disease : - Agustino Bassi - M.J Berkeley - Louis Pateur - Joseph Lister - Robert Koch

Robert Kochs Concept

Microorganism must found in cases of disease,

but not in healty individual Suspected microorganism must be can isolated and growth in cultur The same disease must occur when the organism produce by cultur inoculated The same microorganism must found again from individual who have the disease

Cell
Prokariota Eukariota

Species of microbe
Carl Woese and friends study, domain of

organism: 1. Eukaria-include the all of eukariotic organism 2. Bakteria-prokariotic organism that have rRNA bakterial and lipid of the membran (diasil gliserol eter). 3. Archaea- prokariotic organism that have rRNA archaeal and lipid of the membran (isoprenoid gliseral dieter) or derivat of diglesrol tetraeter.

Function the part of cell

Cell wall : protect from citoplasma asmotic
pressure Cell membran or citoplasma membran : transport Ribosom : sintesa of protein Citoplasma : place that found enzym, protein molecul

Cell Wall
Cover the cell content, make a endomembran

system Protect that have permeubel selectif Transport Respon to outter signal Intercellular interaction Biochemistry activity Energy transduction

 Citoplasma : content amino acid, lipid, ion

for metabolism Mitochondria : source of energy (ATP), place for saving calcium ion RE : syntesis of protein, syntesis of steroid hormon, detoxification,place for saving calcium ion.

Apparatus golgi
Transport materi to outter of the cell Glikosilasi Syntesa of proteoglikan

 Lisosom : intracell digest Ribosom: translation

THANKS FOR ATTENTIONS. ANY QUESTIONS PLEASE