18th APSI Scientists Meet 2009

TLC & HPTLC ANALYSES OF Litsea polyantha JUSS BARK EXTRACTS 11th & 12th October, 2009

Manik Ghosh & B. N. Sinha

Department of Pharmaceutical Sciences, Birla Institute of Technology, Mesra, Ranchi - 835215

OBJECTIVES FOR UNDERTAKING THE WORK Absorption Maxima ( λ max

) of different Spots observed on HPTLC
The bark of Litsea polyantha Juss (Lauraceae) has a long history of Extract Petroleum Chloroform Ethyl acetate Methanol
medicinal use among the traditional healers of Oraon and Munda ether
community of Jharkhand. In Folklore medicines – The bark of L.
polyantha Juss is reported to be used for diarrhea. Powdered bark and Spot Hot Hot Hot Hot
roots are used in external applications for pains, bruises and 1 306 271 219
contusions; they are also used for fractures in animals. The present
study is carried out to analyze the Phytoconstituents present in Litsea 2 285 317 282
polyantha Juss. Bark extracts by TLC & HPTLC.
3 311 306

EXTRACTION 4 257 220

The dried and powdered plant materials were subjected to successive 5 372
hot extraction with Petroleum Ether, Chloroform, Ethyl Acetate and
Methanol to afford respective extracts. 6 255
7 282 282
PHYTOCHEMISTRY 8 206
A preliminary phytochemical investigation of the extracts revealed the
presence of Sterols, Phenols, Flavanoids, Terpenes and Alkaloids. 9 233 301 200
10 287 204
MATERIALS AND METHODS 11 209
Instrument: . Camag HPTLC
Band Applicator: Linomat IV automated spray-on band applicator
equipped with a 100-GL syringe
HPTLC Plates: Precoated Silica Gel 60 F254 Plates (20 x 10 cm)
Scanner: Camag HPTLC Scanner 3
Software: CATS 4.0

PROCEDURE
Petroleum Ether (PELP), Chloroform (CELP) , Ethyl acetate (EELP)
and Methanol (MELP) Extracts were analyzed by TLC & HPTLC

The spots / bands were monitored on UV light (254 nm & 365 nm),
iodine chamber and by using various spraying reagents for detection of
the phytoconstituents..

TLC ANALYSIS OF DIFFERENT EXTRACTS

Extract Solvent System Ratio Rf Values

Petroleum ether Chloroform : Benzene 10 : 1 0.69, 0.81

(60-80)
Chloroform Chloroform : Benzene 10 : 1 0.39, 0.56, 0.72,
0.81, 0.9
Ethyl acetate Chloroform : Ethyl acetate 1:1 0.58, 0.72

Methanol Methanol : Benzene : 9 : 1 : 0.2 0.20, 0.39, 0.56,

Ammonia 0.65, 0.72, 0.82

OH
O
HPTLC ANALYSIS OF DIFFERENT EXTRACTS OH
NH
O
Extract Solvent System Ratio Rf Values HO O

Petroleum ether Chloroform : Benzene 10 : 1 0.71, 0.78

(60-80) OH
O
Chloroform Chloroform : Benzene 10 : 1 0.37, 0.40, 0.45, OH O OH
0.52, 0.58, 0.64,
Quercetin Actinodaphine
0.69, 0.75, 0.82
Ethyl acetate Chloroform : Ethyl acetate 1:1 0.62, 0.74, 0.76 ACKNOWLEDGEMENT
Methanol Methanol : Benzene : 9 : 1 : 0.2 0.23, 0.28, 0.44, The authors would like to thanks Vice Chancellor, Birla Institute of
Ammonia 0.57, 0.65, 0.74, Technology and Head, Department of Pharmaceutical Sciences for
0.81, 0.85 providing the required facilities to carry out this work.