PRACTICAL APPROACHES FOR SEED QUALITY ASSURANCE

I. Seed sampling (2 ! " da#s a$%e& 'a&(es%)* +,lle+% samples pla+ed in an# ,$ %'e $,ll,-ing. +an/ plas%i+ 0ag ,& an# s1i%a0le +,n%aine&.
Hand method Draw a handful of seed from each bag (assuming 50g/handful). Mix thoroughly the seed samples taken from each bag.

Fa0&i+a%ed T&ie& (2am0,, T&ie&/ app&,3ima%el# 45 in+'es l,ng/ 4 in+' diame%e&)

II. Seed Tes%ing

A. 6,is%1&e C,n%en% De%e&mina%i,n ( -,&7ing sample ,$ 8 g) 49: 6C (6a3im1m) Seed Ce&%i$i+a%i,n S%anda&d

4. Seed 0i%ing me%',d (a% leas% 8 de'1lled g&ains)

;%i+7< s,1nd is p&,d1+ed 1p,n 0&ea7ing ,$ g&ain ( app&,3ima%el# 49: 6C)

2. Seed s+&a%+'ing me%',d 1sing $la% s%,ne ,n &,1g' s1&$a+e* (app&,3ima%el# 2 'eap$1l %sp ,$ seeds/ 4 %sp pe& &epli+a%i,n)
S+&a%+'ed seed in -',le g&ain app&,3ima%el# 49: 6C* p1l(e&i=ed g&ain a$%e& s+&a%+'ing app&,3ima%el# m,&e %'an 49: 6C

". Feel 6e%',d

Insert hand approximately up to the middle of the sack If the seeds are warm, humid and adhere on the skin, it indicates above !" #$.

9. O+1la& seed inspe+%i,n $,& m,lds/ 'ea(# seed dis+,l,&a%i,n/ s%i+7iness indi+a%e 'ig' m,is%1&e +,n%en%.

Re+,mmenda%i,n. Re>d&#ing is ad(ised i$ 6C is a0,(e 49:.

2. P'#si+al P1&i%# Tes% (-,&7ing sample ,$ 9 g/ app&,3ima%el# 4 'eap$1l %sp) ?5: P1&i%# (6inim1m) Seed Ce&%i$i+a%i,n S%anda&d

4. 6an1al sepa&a%i,n ,$ imp1&i%ies 'and s,&%ing s%,nes/ s,il/ sand/ -eed seeds/ ,%'e& +&,p seeds/ and ,%'e& plan% pa&%s p&esen%)
P1&e Seed @ ?5: Ine&% 6a%%e& @ 2: ( app&,3ima%el# 4A8 %sp)

Re+,mmenda%i,n. Re+leaning is ad(ised i$ ine&% ma%%e& is m,&e %'an 4A8 %sp ,& .5 gm

C. Be&mina%i,n Tes%
seedsA&epli+a%e -i%' %-, (2) &epli+a%i,ns 58: ge&mina%i,n (Seed +e&%i$i+a%i,n s%anda&d) Seedling e(al1a%i,n a$%e& 8> 49 da#s

>4

4. Rag d,ll me%',d

a. A0s,&0en% Cl,%' 0. Plain pape& +. Hand T,-el Ce% %'e media and sp&ead ,n a $la% s1&$a+e. S,- 4 seeds pe& &epli+a%e and +,(e& -i%' ,ne la#e& ,$ %'e media. R,ll %'e media %, ma7e a &ag d,ll and pla+e in plas%i+ 0ag %, main%ain m,is%1&e. Pla+e %'e &ag d,ll in slan%ing p,si%i,n a%

a. A0s,&0en% Cl,%'

0. Plain Pape&

+. Hand T,-el

2. Sand 6e%',dAS,il 6e%',d

of sand/soil for ! hrs for sterili%ation. &et cool and wet the sand/soil enough to make a ball. If the ball breaks when tapped, it indicates that the sand/soil is suitable for germination. 'ow 00 seeds per replicate and cover with thin layer of sand/soil. $over with plastic or other materials to maintain moisture. Re+,mmenda%i,n. Re%es% i$ ge&mina%i,n is l,- d1e %, $&es'/ and 1nge&mina%ed seeds . I$ ge&mina%i,n is 0el,- 58: / in+&ease seeding &a%e a++,&dingl#.

$ollect and sundry

oil can

D.Da&ie%al Tes%
C,&7ing sample ,$ 28 g&ams@ 4 / g&ains ('al$ sample ,$ %'e &eE1i&ed seed +e&%i$i+a%i,n s%anda&d) 4 g&ains ,$ ,%'e& (a&ie%ies pe& -,&7ing sample ( in0&ed) is all,-ed.

1. Visual inspection based on seed morphology. 2. Separate mixtures from true-to-type variety (inherent characteristics of the variety). 3. etermine the level of mixture.

C,n(e&si,n Ta0le $,& Seed Tes%ing ReE1i&emen%s

Seed Tes%
C,mple%e Tes%

6e%&i+ (App&,3ima%e)
" g&ams

P&a+%i+al
F 'and$1ls ,& " 'eap$1l small sa&dine +ans 4 'eap$1l %sp 4 'and$1l 2 seeds

Rema&7s

6,is%1&e C,n%en%

8 g&ams

P'#si+al P1&i%# 8 g&ams Be&mina%i,n Da&ie%al P1&i%# 8 g&ams 28 g&ams

6an1al C,1n%ing

8 'and$1ls

C,n(e&si,n Ta0le (O%'e& 6eas1&emen%)

Indi+a%,&
2agging

6e%&i+
9 7g

P&a+%i+al
9 ,il +ans

Rema&7s
App&,3ima%el# " gall,ns Heap$1l

Bene&al 4 6eas1&emen%

g&ams

4 small sa&dine +an (488g) 4 g&ains

Bene&al 28 g&ams 6eas1&emen% Bene&al 9 g&ams 6eas1&emen%

4 %sp

Thank you

CORN
Ass1mp%i,ns.
(istribution of kg registered seeds to produce ) bags * +kg/bag) ,eneficiaries- .armers who don/t have access on certified seeds. $rop $ulture- 0pland 1ainfed 2nowledge level- #inimal on seed technology*basic seed testing techni3ues) 4uality 5ssurance on the crop'eed &evel 'eeds properly dried at approximately !" #oisture content before bagging and storage

I. Seed sampling
5fter processing, collect samples and place in any of the following- can, plastic bag or any suitable container.

6and method (raw two * 7) handfuls sample from each bag *assuming 55g/handful). #ix thoroughly the seed samples taken from each bag.

Fa0&i+a%ed T&ie& (2am0,, T&ie&/ app&,3ima%el# 45 in+'es l,ng/ 4 in+' diame%e&)

II. Seed Tes%ing

A. 6,is%1&e C,n%en% De%e&mina%i,n ( -,&7ing sample ,$ 4G g) 49: 6C (6a3im1m) Seed Ce&%i$i+a%i,n S%anda&d

4. Seed 0i%ing me%',d (a% leas% 8 7e&nels)

8tick9 sound is produced upon breaking of grain * approximately !" #$)

2. 2,%%le 6e%',d

:ut ; handfuls of corn kernel in a small vinegar glass bottle and cover with plastic cap or plastic bag sealed with rubber band. <xpose under the sun for 7 hrs. =bserve the presence or absence of moisture inside the bottle. >he presence of moisture indicates high moisture content.

9. O+1la& seed inspe+%i,n $,& m,lds 'ea(# seed

Re+,mmenda%i,n. Re>d&#ing is ad(ised i$ 6C is a0,(e 49:.

". Feel 6e%',d

Insert hand approximately up to the middle of the sack. If the seeds are warm and humid, it indicates above !" #$.

2. P'#si+al P1&i%# Tes% (-,&7ing sample ,$ 9 g/ app&,3ima%el# G 'and$1ls) 5: P1&i%# (6inim1m) Seed Ce&%i$i+a%i,n S%anda&d

4. 6an1al sepa&a%i,n ,$ imp1&i%ies > 'and s,&%ing s%,nes/ s,il/ sand/ -eed seeds/ ,%'e& +&,p seeds/ and ,%'e& plan% pa&%s p&esen%)
P1&e Seed @ ?5: Ine&% 6a%%e& @ 2: ( app&,3ima%el# 2 %0sp)

Re+,mmenda%i,n. Re>+leaning is ad(ised i$ ine&% ma%%e& is m,&e %'an 2 %0sp.

C. Be&mina%i,n Tes%
>( 8 seedsA &epli+a%e -i%' 2 &epli+a%i,ns) 58: ge&mina%i,n (Seed +e&%i$i+a%i,n s%anda&d) Seedling e(al1a%i,n a$%e& 8>G da#s 4. Rag d,ll me%',d a. 5bsorbent $loth b. :lain paper c. 6and >owel ?et the media and spread on a flat surface. 'ow 50 seeds per replicate and cover with one layer of the media. 1oll the media to make a rag doll and place in plastic bag to maintain moisture. :lace the rag doll in slanting position at room temperature.

a. A0s,&0en% Cl,%'

Ce% %'e media and sp&ead ,n a $la% s1&$a+e.

S,- 8 seeds pe& &epli+a%e and +,(e& -i%' ,ne la#e& ,$ %'e media.

R,ll %'e media %, ma7e a &ag d,ll and pla+e in plas%i+ 0ag %, main%ain m,is%1&e.

Pla+e %'e &ag d,ll in slan%ing p,si%i,n

a% &,,m %empe&a%1&e

7. 'and #ethod/'oil #ethod

C,lle+% and s1nd&# 4 ,il +an ,$

sandAs,il $,& 9 '&s $,& s%e&ili=a%i,n.

Le% +,,l and -e% %'e sandAs,il en,1g' %, ma7e a 0all.

S,- 8 seeds pe& &epli+a%e and +,(e& C,(e& -i%' plas%i+ ,& ,%'e& ma%e&ials %, -i%' %'in la#e& ,$ sandAs,il. main%ain m,is%1&e.

Re+,mmenda%i,n. Re%es% i$ ge&mina%i,n is l,- d1e %, $&es'/ and 1nge&mina%ed seeds . I$ ge&mina%i,n is 0el,- 58: / in+&ease seeding &a%e a++,&dingl#.

D. Da&ie%al Tes%
C,&7ing sample ,$ 9 g&ams@ G 'and$1ls ('al$ sample ,$ %'e &eE1i&ed seed +e&%i$i+a%i,n s%anda&d) 8 7e&nels ,$ ,%'e& (a&ie%ies pe& 4.Dis1al inspe+%i,n 0ased ,n seed -,&7ing sample is all,-ed.

Re+,mmenda%i,n. I$ m,&e %'an 8 7e&nels ,$ ,%'e& (a&ie%ies pe& -,&7ing sample is $,1nd/ +,nd1+% %a0le s,&%ing %, dis+a&d mi3%1&es.

m,&p',l,g#. 2.Sepa&a%e mi3%1&es $&,m %&1e>%,>%#pe (a&ie%# (in'e&en% +'a&a+%e&is%i+s ,$ %'e (a&ie%#). ".De%e&mine %'e le(el ,$ mi3%1&e.

C,n(e&si,n Ta0le $,& Seed Tes%ing ReE1i&emen%s

'eed >est
$omplete >est #oisture $ontent :hysical :urityA Bermination Carietal :urityA

#etric *5pproximate)
)00 grams @0 grams !00 grams ;0 grams !00 grams

:ractical
0 handfuls ; handfuls @ handfuls 00 seeds @ handfuls

1emarks

#anual $ounting

*Same samples for physical and varietal

C,n(e&si,n Ta0le (O%'e& 6eas1&emen%)

Indicator
,agging Beneral #easurement Beneral #easurement Beneral #easurement

#etric
+ kg ;00 grams

:ractical
.5 oil can 000 grains

1emarks

! grams

tbsp

55 grams

handful

File name:Final training materials/mechanics and requirements of seed certification