THE MECHANISMS OF ENZYME

FUNCTION AND INHIBITION

GROUP 2
GROUP MEMBERS
Phan Duc Anh
Nguyen Hien Huy Hoang
Vu Thi My Linh
Nguyen Pham Thang Long
Nguyen Duc Thanh
Nguyen Thi Bao Tran
Nguyen Quynh An Trang
Nguyen Vu Hoang Uyen
CONTENTS
INTRODUCTION
MECHANISM OF ENZYME FUNCTION
MECHANISM OF ENZYME INHIBITION
CONCLUSION
REFERENCES
INTRODUCTION
Enzymes are proteins, they work as biological
catalysts.
INTRODUCTION
The function of enzymes is to accelerate the rate of
reactions.
Enzymes are not consumed by the reaction.

INTRODUCTION
Enzyme inhibitors are used to prevent enzymes from
working. When Enzyme cofactors required for some
enzymes to work.
Applying these
knowledge
to make poisons
or therapeutic
substances.

MECHANISM OF ENZYME
FUNCTION

There are several types of catalytic mechanisms of
enzyme.
Acid base catalysis
Covalent catalysis
Metal ion catalysis
Catalysis by approximation

MECHANISM OF ENZYME
FUNCTION


Lock and key
model
Induced-fit
model
MECHANISM OF ENZYME
INHIBITION

Non-specific
Specific
Irreversible
Group-specific
Affinity label
Suicide
Reversible
Competitive
Non-competitive
Uncompetitive

MECHANISM OF ENZYME
INHIBITION

Non-specific: any physical (temperature) or
chemical (acid and base) changes.
Denature the enzymes
Non-specific inhibitors affect all kind of enzymes in the
same way.
MECHANISM OF ENZYME
INHIBITION

Irreversible: Inhibitors bind (covalently or non-covalently)
to the enzyme so tightly permanently block the
enzymes activity.
The binding site could be at, near, or remote from the
active site.

IRREVERSIBLE
INHIBITORS
Suicide
Affinity
label
Group-
specific
reagents react with specific side
chains of amino acids
are molecules that are
structurally similar to the
substrate for an enzyme and
that covalently bind to active-
site residues
get activated upon binding
with the active site of specific
enzyme by a few steps of the
biochemical reaction with no
product
MECHANISM OF ENZYME
INHIBITION

Competitive inhibitors: The inhibitor competes with the
substrate for the same active site.
MECHANISM OF ENZYME
INHIBITION

Non-competitive inhibitors: A noncompetitive inhibitor is
a substance that interacts with the enzyme OR enzyme-
substrate complex.
MECHANISM OF ENZYME
INHIBITION

Un-competitive inhibitors:
un-competitive inhibitors
just ONLY bind to
the enzyme-substrate
complex.
CONCLUSION
Enzymes are biological catalysts which are proteins

Functions of enzymes are to increase the reaction rate

Inhibitors could be just to impair the function of enzymes


REFERENCES
Jeremy M. Berg, John L. Tymoczko, Lubert Stryer and Gregory
J. Gatto, Jr. Biochemistry. 7
th
edition. New York: W. H. Freeman
and Company, 2012.
Donald Voet, Judith G. Voet and Charlotte W. Pratt.
Fundamentals of Biochemistry: Life at the Molecular Level, 3th
edition. The United State of America: KendallVille, 2008
Geoffrey M. Cooper. The Cell: A Molecular Approach. 2
nd

edition. Sinauer Associates, 2000
Enzyme, Wikipedia. 2013
http://en.wikipedia.org/wiki/Enzyme (Accessed 06-Oct-2013)
http://www.ncbi.nlm.nih.gov/books/NBK9921/