Glyphosates Suppression of

Cytochrome P450 Enzymes and

Amino Acid Biosynthesis by the
Gut Microbiome: Pathways to
Modern Diseases

Anthony Samsel and Stephanie Seneff
INTRODUCTION
INTRODUCTION
Roundup
- A herbicide
-Contains adjuvant to make glyphosate
more potent
-In Philippines setting:
Agriculture (promoted by the Agriculture and
Agrarian reform officials)
Forestry
Lawn
Garden and Aquatic Environment
INTRODUCTION
Roundup
-Glyphosate (N-phosphonomethylglycine)
active ingredient
metabolized into aminomethylphosphonic
acid (AMPA) in the human body
administered shortly before harvest and crop
desiccation
Claimed to be less toxic than aspirin
(measurement of its presence in food is practically
nonexistent)
INTRODUCTION
Roundup
-Glyphosate (N-phosphonomethylglycine)
claimed MOA in plants is the disruption of
Shikimate pathway
currently accepted dogma:
Not harmful to humans or any mammals
because Shikimate pathway is not present.
INTRODUCTION
Roundup
-Glyphosate (N-phosphonomethylglycine)
Shikimate pathway is present in gut bacteria
Gut bacteria
aid in digestion
synthesis of vitamins
detoxify xenobiotics
participate in immune system homeostasis
gastrointestinal tract permeability
DISCUSSION
DISCUSSION
GLYPHOSATE
-Pathological biological effects are:
disruption of the gut bacteria

impairment of the sulfate transport

interference with the CYP enzyme
activity
DISCUSSION
Disruption of the gut bacteria
Increase in short chain fatty acids
Increase in ammonia
- Autism Spectrum Disorder
- Hepatic Encephalopathy
- Acute and Chronic Liver
Dysfunction
- Intestinal Diseases
DISCUSSION
Impairment of the sulfate transport
Disseminated Intravascular Coagulation
-> salting out of proteins
-> coagulation
Multiple Organ Failure
Autism
Elevated level of pyridoxal phosphate
(Vit.B6)
Colitis and Crohns Disease
-> sulfate depletion in the gut
DISCUSSION
Interference with the CYP enzyme
activity
-Inhibition of aromatase
decreases of 10 ppm HepG2 cells (in
human liver)
more potent effect due to adjuvants
-Enhancement of retinoic acid
increased RA is due to the inhibition of a
CYP enzyme
facial deformities
DISCUSSION
Interference with the CYP enzyme
activity
-Enhancement of retinoic acid
increased RA is due to the inhibition of a
CYP enzyme (noted among
organophosphates)
facial deformities

DISCUSSION
Developmental Immunotoxicity
Autism
Inflammatory Bowel Disease
Obesity
Cardiovascular Disease
Depression
Cancer
Cachexia
Alzheimers Disease
DISCUSSION
Parkinsons Disease
Multiple Sclerosis
Amyotropic Lateral Sclerosis (ALS)
CONCLUSION
CONCLUSION
Adverse effect on the gut microbiota
Established ability to inhibit the activity of
cytochrome P450 enzyme
Impairment of sulfate transport
-> can remarkably explain a great number of
the diseases and conditions that are prevalent in
the modern industrialized world
With insidious effect
Contrary to being nontoxic, it may in fact be the
most biologically disruptive chemical in our
environment