Microbiology laboratoric test

Gram Stain
Material
solutions of crystal violet, Grams
iodine (2 g potassium iodide in
300 ml distilled water plus 1 g
iodine crystals), 95% ethanol
and/or
isopropanol-acetone mixture
(3:1 v/v), and
safranin
Bismark brown stain (for color-
blind students)
clean glass slides
inoculating loop
Bunsen burner
bibulous paper
microscope
lens paper and lens cleaner
immersion oil
Hyphomonas (Hyphomicrobium)
neptunium (ATCC 15444) grown
in marine broth (Difco)
slide warmer
staining rack
Bacto Gram Stain Reagents from
Difco for the three-step Gram
stain

Gram Stain

Gram Stain

Gram Stain

Gram Stain

Gram Stain

Gram Stain

Gram Stain

Gram Stain

Gram Stain

Gram Stain
Examination the preparation under microscope ( x100) for confirmation
Microscopic result for Gram Stain
Microbiology laboratoric test
KOH staining
Direct microscopic examination of the specimens can
be performed using potassium hydroxide preparations.
Skin debris is placed on a glass slide
A drop of a 10%-30% KOH solution with ink (3 ml of
KOH solution mixed with 1 cartridge of ink) added.
After applying a cover-slip, the slide is placed in a moist
chamber for 10 15 minutes to permit clearing of the
keratin
It is then viewed under a microscope.
Specimen collection for KOH staining
Desinfectant with alcohol 70 %
Specimen collection for KOH staining
Scrape the scale from the edge of the scaling lesion
onto a microscopic slide


Specimen collection for KOH staining
Place no more than one or two drops of 10% KOH


Specimen collection for KOH staining
Covering with coverslip
Specimen collection for KOH staining
gentle warming will accelerate the dissolution of celluler debris
Specimen collection for KOH staining
Examination the preparation under microscope use low
illumination (x10 and x 40) for confirmation
Specimen collection for KOH staining
P.versicolor
Desinfectant with alcohol 70 %
Specimen collection for KOH staining
P.versicolor
Remove scale with tape
Specimen collection for KOH staining
P.versiclor
Covering the object glass with tape
Specimen collection for KOH staining - TV
Place no more than one or two drops of 10% KOH


Specimen collection for KOH staining - TV
Examination the preparation under microscope use low
illumination (x10 and x 40) for confirmation
Microscopic result for KOH staining

Microscopic result for KOH staining
Microscopic result for KOH staining