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SIZE OF BACTERIA
Cocci: sphere, 1m
Bacilli: rods , 0.5-1 m in width -3 m in length
Spiral bacteria: 1~3 m in length and 0.3-0.6 m in width
Structure of Bacteria
Essential structures
cell wall
cell membrane
Cytoplasm
nuclear material
Particular structures
capsule
flagella
pili
spore
1884: Christian Gram: First publication for the Gram stain method)
Editor's note: I would like to testify that I have found the Gram method to be one of
the best and for many cases the best method which I have ever used for staining
Schizomycetes.
Flagellum
Cell membrane Nucleoid
Cell wall
Gram +
Pili
Gram Granule
Capsule
Cell (inner) membrane Outer membrane
Ribosomes
Cell wall
Cell wall
Situation:
outmost portion.
15-30nm in
thickness, 10%25% of dry
weight.
Special components of
Gram positive cell wall
Teichoic acid
SPA / M POTEIN
Special components of
Gram negative cell wall
When bacteria are treated with 1) enzymes that are lytic for
the cell wall e.g. lysozyme or 2) antibiotics that interfere
with biosynthesis of peptidoglycan, wall-less bacteria are
often produced.
Usually these treatments generate non-viable organisms.
Wall-less bacteria that can not replicate are referred to as
spheroplasts (when an outer membrane is present) or
protoplasts (if an outer membrane is not present).
Occasionally wall-less bacteria that can replicate are
generated by these treatments (L forms).
Cell
membrane
Site of biosynthesis of DNA, cell wall polymers and membrane lipids. Selective
permeability and transport of solutes into cells
Electron transport and oxidative phosphorylation
Excretion of hydrolytic exoenzymes
Mesosomes
Cytoplasm
Plasmids: extrachromosomal
genetic elements
Inclusions: sources of stored
energy, e,g volutin
Plasmid
granulose
Inclusions are
aggregates of various
compounds that are
normally involved in
storing energy
reserves or building
blocks for the cell.
Inclusions accumilate
when a cell is grown
in the presence of
excess nutrients and
they are often
observed under
laboratory conditions.
Inclusions of
Bacteria
Nucleus
Lacking nuclear
membrane, absence
of nucleoli, hence
known as nucleic
material or nucleoid,
one to several per
bacterium.
Attachment
Protection from phagocytic
engulfment.
Resistance to drying.
Depot for waste products.
Reservoir for certain
nutrients.
protection
These are structures surrounding the outside of the cell envelope. They
usually consist of polysaccharide; however, in certain bacilli they are
composed of a polypeptide (polyglutamic acid). They are not essential
to cell viability and some strains within a species will produce a capsule,
whilst others do not. Capsules are often lost during in vitro culture.
Some bacterial
Identification
of Bacteria
Pathogenesis
Motility of
bacteria
Monotrichate/Amphitrichate/Lophotrichate/Peritrichate
Flagella
Pili
Pili are hair-like projections of the cell , They are
known to be receptors for certain bacterial viruses.
Chemical nature is pilin
Classification and Function
a. Common pili or fimbriae: fine , rigid numerous,
related to bacterial adhesion
b. Sex pili: longer and coarser, only 1-4, related to
bacterial conjugation
Endospores
(spores)
Dormant cell
Resistant to adverse
conditions
- high temperatures
- organic solvents
Identification of
Bacteria
Pathogenesis
Resistance
Methods
Microscope
Light Microscope
Electron Microscope
Darkfield Microscope
Phase Contrast Microscope
Fluorescence Microscope
Cofocal Microscope
Staining Methods
Simple staining;
Differential staining ( Gram
stain, Acid-fast stain),
Special staining( Negative stain,
Spore stain, Flagella stain)