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Chapter 6

A Tour of the Cell

PowerPoint Lectures for


Biology, Seventh Edition
Neil Campbell and Jane Reece

Lectures by Chris Romero


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Overview: The Importance of Cells


All organisms are made of cells
The cell is the simplest collection of matter
that can live

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Cell structure is correlated to cellular function

Figure 6.1
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10 m

Concept 6.1: To study cells, biologists use


microscopes and the tools of biochemistry

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Microscopy
Scientists use microscopes to visualize cells
too small to see with the naked eye

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Light microscopes (LMs)


Pass visible light through a specimen
Magnify cellular structures with lenses

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Unaided eye

Different types of microscopes


Can be used to visualize different sized
cellular structures
10 m

Length of some
nerve and
muscle cells
Chicken egg

1 cm

100 m
10 m
1m
100 nm

Frog egg

Most plant
and Animal
cells
Nucleus
Most bacteria
Mitochondrion

Smallest bacteria
Viruses

10 nm

Ribosomes
Proteins

1 nm

Lipids
Small molecules

Figure 6.2

0.1 nm

Atoms

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Electron microscope

1 mm

Electron microscope

0.1 m

Human height

Light microscope

1m

Measurements
1 centimeter (cm) = 102 meter (m) = 0.4 inch
1 millimeter (mm) = 103 m
1 micrometer (m) = 103 mm = 106 m
1 nanometer (nm) = 103 mm = 109 m

Use different methods for enhancing


visualization of cellular structures
TECHNIQUE

RESULT

(a) Brightfield (unstained specimen).

Passes light directly through specimen.


Unless cell is naturally pigmented or
artificially stained, image has little
contrast. [Parts (a)(d) show a
human cheek epithelial cell.]
50 m

(b) Brightfield (stained specimen).


Staining with various dyes enhances
contrast, but most staining procedures
require that cells be fixed (preserved).

(c) Phase-contrast. Enhances contrast


in unstained cells by amplifying
variations in density within specimen;
especially useful for examining living,
unpigmented cells.

Figure 6.3
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(d) Differential-interference-contrast (Nomarski).


Like phase-contrast microscopy, it uses optical
modifications to exaggerate differences in
density, making the image appear almost 3D.
(e) Fluorescence. Shows the locations of specific
molecules in the cell by tagging the molecules
with fluorescent dyes or antibodies. These
fluorescent substances absorb ultraviolet
radiation and emit visible light, as shown
here in a cell from an artery.
(f) Confocal. Uses lasers and special optics for
optical sectioning of fluorescently-stained
specimens. Only a single plane of focus is
illuminated; out-of-focus fluorescence above
and below the plane is subtracted by a computer.
A sharp image results, as seen in stained nervous
tissue (top), where nerve cells are green, support
cells are red, and regions of overlap are yellow. A
standard fluorescence micrograph (bottom) of this
relatively thick tissue is blurry.

50 m

50 m
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Electron microscopes (EMs)


Focus a beam of electrons through a specimen
(TEM) or onto its surface (SEM)

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The scanning electron microscope (SEM)


Provides for detailed study of the surface of a
specimen
TECHNIQUE

RESULTS
1 m

Cilia
(a) Scanning electron microscopy (SEM). Micrographs taken
with a scanning electron microscope show a 3D image of the
surface of a specimen. This SEM
shows the surface of a cell from a
rabbit trachea (windpipe) covered
with motile organelles called cilia.
Beating of the cilia helps move
inhaled debris upward toward
the throat.

Figure 6.4 (a)


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The transmission electron microscope (TEM)


Provides for detailed study of the internal
ultrastructure of cells
Longitudinal
section of
cilium
(b) Transmission electron microscopy (TEM). A transmission electron
microscope profiles a thin section of a
specimen. Here we see a section through
a tracheal cell, revealing its ultrastructure.
In preparing the TEM, some cilia were cut
along their lengths, creating longitudinal
sections, while other cilia were cut straight
across, creating cross sections.

Figure 6.4 (b)


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Cross section
of cilium

1 m

Isolating Organelles by Cell Fractionation


Cell fractionation
Takes cells apart and separates the major
organelles from one another

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The centrifuge
Is used to fractionate cells into their
component parts

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The process of cell fractionation


APPLICATION

Cell fractionation is used to isolate


(fractionate) cell components, based on size and density.
TECHNIQUE

First, cells are homogenized in a blender to


break them up. The resulting mixture (cell homogenate) is then
centrifuged at various speeds and durations to fractionate the cell
components, forming a series of pellets.

Figure 6.5
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Homogenization

Tissue
cells

1000 g
Homogenate
(1000 times the
force of gravity)
Differential centrifugation
10 min
Supernatant poured
into next tube
20,000 g
20 min

Pellet rich in
nuclei and
cellular debris

Figure 6.5

80,000 g
60 min
150,000 g
3 hr

Pellet rich in
mitochondria
(and chloroplasts if cells
are from a Pellet rich in
plant)
microsomes
(pieces of
plasma membranes and
Pellet rich in
cells internal ribosomes
membranes)

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In the original experiments, the researchers


used microscopy to identify the organelles in each pellet,
establishing a baseline for further experiments. In the next series of
experiments, researchers used biochemical methods to determine
the metabolic functions associated with each type of organelle.
Researchers currently use cell fractionation to isolate particular
organelles in order to study further details of their function.
RESULTS

Figure 6.5
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Concept 6.2: Eukaryotic cells have internal


membranes that compartmentalize their functions
Two types of cells make up every organism
Prokaryotic
Eukaryotic

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Comparing Prokaryotic and Eukaryotic Cells


All cells have several basic features in common
They are bounded by a plasma membrane

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They contain a semifluid substance called the


cytosol
They contain chromosomes
They all have ribosomes

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Prokaryotic cells
Do not contain a nucleus
Have their DNA located in a region called
the nucleoid

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Pili: attachment structures on


the surface of some prokaryotes
Nucleoid: region where the
cells DNA is located (not
enclosed by a membrane)
Ribosomes: organelles that
synthesize proteins

Bacterial
chromosome
(a) A typical
rod-shaped bacterium

Plasma membrane: membrane


enclosing the cytoplasm
Cell wall: rigid structure outside
the plasma membrane
Capsule: jelly-like outer coating
of many prokaryotes
0.5 m
Flagella: locomotion
organelles of
some bacteria

Figure 6.6 A, B
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(b) A thin section through the


bacterium Bacillus coagulans
(TEM)

Eukaryotic cells
Contain a true nucleus, bounded by a
membranous nuclear envelope
Are generally quite a bit bigger than
prokaryotic cells

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The logistics of carrying out cellular


metabolism sets limits on the size of cells

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A smaller cell
Has a higher surface to volume ratio, which
facilitates the exchange of materials into and
out of the cell
Surface area increases while
total volume remains constant

5
1
1
Total surface area
(height width
number of sides
number of boxes)

150

750

Total volume
(height width length
number of boxes)

125

125

Surface-to-volume
ratio
(surface area volume)

12

Figure 6.7
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The plasma membrane


Functions as a selective barrier
Allows sufficient passage of nutrients
and waste
Outside of cell

Carbohydrate side chain

Hydrophilic
region
Inside of cell

Figure 6.8 A, B

0.1 m

(a) TEM of a plasma


membrane. The
plasma membrane,
here in a red blood
cell, appears as a
pair of dark bands
separated by a
light band.

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Hydrophobic
region
Hydrophilic
region

Phospholipid

Proteins

(b) Structure of the plasma membrane

A Panoramic View of the Eukaryotic Cell


Eukaryotic cells
Have extensive and elaborately arranged
internal membranes, which form organelles

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Plant and animal cells


Have most of the same organelles

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A animal cell
ENDOPLASMIC RETICULUM (ER)

Rough ER

Smooth ER

Nuclear envelope
Nucleolus

NUCLEUS

Chromatin

Flagelium

Plasma membrane

Centrosome

CYTOSKELETON
Microfilaments
Intermediate filaments
Ribosomes

Microtubules
Microvilli

Golgi apparatus
Peroxisome
Figure 6.9

Mitochondrion

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Lysosome

In animal cells but not plant cells:


Lysosomes
Centrioles
Flagella (in some plant sperm)

A plant cell

Nuclear envelope
Nucleolus
Chromatin

NUCLEUS

Centrosome

Rough
endoplasmic
reticulum Smooth
endoplasmic
reticulum

Ribosomes (small brwon dots)

Central vacuole
Tonoplast

Golgi apparatus

Microfilaments
Intermediate
filaments

CYTOSKELETON

Microtubules

Mitochondrion
Peroxisome
Plasma membrane
Chloroplast

Cell wall
Plasmodesmata
Wall of adjacent cell

Figure 6.9
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In plant cells but not animal cells:


Chloroplasts
Central vacuole and tonoplast
Cell wall
Plasmodesmata

Concept 6.3: The eukaryotic cells genetic


instructions are housed in the nucleus and
carried out by the ribosomes

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The Nucleus: Genetic Library of the Cell


The nucleus
Contains most of the genes in the
eukaryotic cell

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The nuclear envelope


Encloses the nucleus, separating its contents
from the cytoplasm
Nucleus

1 m

Nucleolus
Chromatin

Nucleus

Nuclear envelope:
Inner membrane
Outer membrane
Nuclear pore
Pore
complex
Rough ER
Surface of nuclear
envelope.

1 m

Ribosome

0.25 m

Close-up of
nuclear
envelope

Figure 6.10

Pore complexes (TEM).

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Nuclear lamina (TEM).

Ribosomes: Protein Factories in the Cell


Ribosomes
Are particles made of ribosomal RNA
and protein

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Carry out protein synthesis


Ribosomes

ER

Cytosol
Endoplasmic reticulum (ER)
Free ribosomes

Bound ribosomes
Large
subunit

0.5 m
TEM showing ER and ribosomes

Figure 6.11
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Small
subunit
Diagram of a ribosome

Concept 6.4: The endomembrane system


regulates protein traffic and performs metabolic
functions in the cell
The endomembrane system
Includes many different structures

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The Endoplasmic Reticulum: Biosynthetic Factory


The endoplasmic reticulum (ER)
Accounts for more than half the total
membrane in many eukaryotic cells

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The ER membrane
Is continuous with the nuclear envelope
Smooth ER
Rough ER

Nuclear
envelope

ER lumen
Cisternae
Ribosomes
Transitional ER
Transport vesicle
Smooth ER
Rough ER 200 m

Figure 6.12
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There are two distinct regions of ER


Smooth ER, which lacks ribosomes
Rough ER, which contains ribosomes

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Functions of Smooth ER
The smooth ER
Synthesizes lipids
Metabolizes carbohydrates
Stores calcium
Detoxifies poison

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Functions of Rough ER
The rough ER
Has bound ribosomes
Produces proteins and membranes, which are
distributed by transport vesicles

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The Golgi Apparatus: Shipping and


Receiving Center
The Golgi apparatus
Receives many of the transport vesicles
produced in the rough ER
Consists of flattened membranous sacs called
cisternae

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Functions of the Golgi apparatus include


Modification of the products of the rough ER
Manufacture of certain macromolecules

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Functions of the Golgi apparatus


Golgi
apparatus
cis face
(receiving side of
Golgi apparatus)

1 Vesicles move
2 Vesicles coalesce to
6 Vesicles also
form new cis Golgi cisternae
from ER to Golgi
transport certain
Cisternae
proteins back to ER
3 Cisternal
maturation:
Golgi cisternae
move in a cisto-trans
direction

Figure 6.13
5 Vesicles transport specific
proteins backward to newer
Golgi cisternae

4 Vesicles form and


leave Golgi, carrying
specific proteins to
other locations or to
the plasma membrane for secretion

trans face
(shipping side of
Golgi apparatus)

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0.1 0 m

TEM of Golgi apparatus

Lysosomes: Digestive Compartments


A lysosome
Is a membranous sac of hydrolytic enzymes
Can digest all kinds of macromolecules

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Lysosomes carry out intracellular digestion by


Phagocytosis
Nucleus

1 m

Lysosome

Lysosome contains
active hydrolytic
enzymes

Food vacuole
fuses with
lysosome

Hydrolytic
enzymes digest
food particles

Digestive
enzymes
Lysosome
Plasma membrane
Digestion
Food vacuole

Figure 6.14 A
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(a) Phagocytosis: lysosome digesting food

Autophagy
Lysosome containing
two damaged organelles

1m

Mitochondrion
fragment
Peroxisome
fragment

Lysosome fuses with


vesicle containing
damaged organelle

Hydrolytic enzymes
digest organelle
components

Lysosome

Figure 6.14 B

Vesicle containing
damaged mitochondrion

Digestion

(b) Autophagy: lysosome breaking down damaged organelle

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Vacuoles: Diverse Maintenance Compartments


A plant or fungal cell
May have one or several vacuoles

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Food vacuoles
Are formed by phagocytosis

Contractile vacuoles
Pump excess water out of protist cells

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Central vacuoles
Are found in plant cells
Hold reserves of important organic
compounds and water
Central vacuole
Cytosol
Tonoplast

Nucleus

Central
vacuole

Cell wall
Chloroplast
Figure 6.15
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5 m

The Endomembrane System: A Review


The endomembrane system
Is a complex and dynamic player in the cells
compartmental organization

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Relationships among organelles of the


endomembrane system
1 Nuclear envelope is
connected to rough ER,
which is also continuous
with smooth ER

Nucleus

Rough ER

Membranes and proteins


produced by the ER flow in
the form of transport vesicles
to the Golgi

Smooth ER

cis Golgi

Nuclear envelop

Golgi pinches off transport


Vesicles and other vesicles
that give rise to lysosomes and
Vacuoles

Plasma
membrane

trans Golgi

Lysosome available
for fusion with another
vesicle for digestion

Figure 6.16
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5 Transport vesicle carries 6


proteins to plasma
membrane for secretion

Plasma membrane expands


by fusion of vesicles; proteins
are secreted from cell

Concept 6.5: Mitochondria and chloroplasts


change energy from one form to another
Mitochondria
Are the sites of cellular respiration

Chloroplasts
Found only in plants, are the sites of
photosynthesis

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Mitochondria: Chemical Energy Conversion


Mitochondria
Are found in nearly all eukaryotic cells

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Mitochondria are enclosed by two membranes


A smooth outer membrane
An inner membrane folded into cristae
Mitochondrion
Intermembrane space
Outer
membrane

Free
ribosomes
in the
mitochondrial
matrix

Inner
membrane
Cristae
Matrix

Figure 6.17

Mitochondrial
DNA

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100 m

Chloroplasts: Capture of Light Energy


The chloroplast
Is a specialized member of a family of closely
related plant organelles called plastids
Contains chlorophyll

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Chloroplasts
Are found in leaves and other green organs of
plants and in algae

Chloroplast

Ribosomes
Stroma

Chloroplast
DNA

Inner and outer


membranes
Granum
1 m

Figure 6.18

Thylakoid

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Chloroplast structure includes


Thylakoids, membranous sacs
Stroma, the internal fluid

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Peroxisomes: Oxidation
Peroxisomes
Produce hydrogen peroxide and convert it to
water
Chloroplast
Peroxisome
Mitochondrion

Figure 6.19

1 m
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Concept 6.6: The cytoskeleton is a network of


fibers that organizes structures and activities in
the cell

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The cytoskeleton
Is a network of fibers extending throughout the
cytoplasm
Microtubule

Figure 6.20
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0.25 m

Microfilaments

Roles of the Cytoskeleton: Support, Motility, and Regulation

The cytoskeleton
Gives mechanical support to the cell

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Is involved in cell motility, which utilizes motor


proteins
ATP

Vesicle
Receptor for
motor protein

Motor protein
Microtubule
(ATP powered)
of cytoskeleton
(a) Motor proteins that attach to receptors on organelles can walk
the organelles along microtubules or, in some cases, microfilaments.
Vesicles
Microtubule
0.25 m

Figure 6.21 A, B

(b) Vesicles containing neurotransmitters migrate to the tips of nerve cell


axons via the mechanism in (a). In this SEM of a squid giant axon, two
vesicles can be seen moving along a microtubule. (A separate part of the
experiment provided the evidence that they were in fact moving.)

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Components of the Cytoskeleton

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There are three main types of fibers that make


up the cytoskeleton

Table 6.1
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Microtubules
Microtubules
Shape the cell
Guide movement of organelles
Help separate the chromosome copies in
dividing cells

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Centrosomes and Centrioles


The centrosome
Is considered to be a microtubule-organizing
center

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Contains a pair of centrioles


Centrosome

Microtubule
Centrioles
0.25 m

Figure 6.22

Longitudinal section
of one centriole

Microtubules

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Cross section
of the other centriole

Cilia and Flagella


Cilia and flagella
Contain specialized arrangements of
microtubules
Are locomotor appendages of some cells

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Flagella beating pattern

(a) Motion of flagella. A flagellum


usually undulates, its snakelike
motion driving a cell in the same
direction as the axis of the
flagellum. Propulsion of a human
sperm cell is an example of
flagellatelocomotion (LM).

Direction of swimming

Figure 6.23 A
1 m

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Ciliary motion

(b) Motion of cilia. Cilia have a backand-forth motion that moves the
cell in a direction perpendicular
to the axis of the cilium. A dense
nap of cilia, beating at a rate of
about 40 to 60 strokes a second,
covers this Colpidium, a
freshwater protozoan (SEM).

Figure 6.23 B
15 m

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Cilia and flagella share a common


ultrastructure
Outer microtubule
doublet
Dynein arms

0.1 m

Central
microtubule
Outer doublets
cross-linking
proteins inside

Microtubules

Radial
spoke

Plasma
membrane
Basal body

0.5 m

(a)

(b)

0.1 m

Triplet

(c)

Figure 6.24 A-C


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Cross section of basal body

Plasma
membrane

The protein dynein


Is responsible for the bending movement of
cilia and flagella
Microtubule
doublets

ATP

Dynein arm
(a) Powered by ATP, the dynein arms of one microtubule doublet
grip the adjacent doublet, push it up, release, and then grip again.
If the two microtubule doublets were not attached, they would slide
relative to each other.
Figure 6.25 A
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ATP

Outer doublets
cross-linking
proteins

Anchorage
in cell

(b) In a cilium or flagellum, two adjacent doublets cannot slide far because
they are physically restrained by proteins, so they bend. (Only two of
the nine outer doublets in Figure 6.24b are shown here.)
Figure 6.25 B
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(c) Localized, synchronized activation of many dynein arms


probably causes a bend to begin at the base of the Cilium or
flagellum and move outward toward the tip. Many successive
bends, such as the ones shown here to the left and right,
result in a wavelike motion. In this diagram, the two central
microtubules and the cross-linking proteins are not shown.
Figure 6.25 C

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Microfilaments (Actin Filaments)


Microfilaments
Are built from molecules of the protein actin

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Are found in microvilli


Microvillus

Plasma membrane

Microfilaments (actin
filaments)

Intermediate filaments
Figure 6.26
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0.25 m

Microfilaments that function in cellular motility


Contain the protein myosin in addition to actin

Muscle cell
Actin filament

Myosin filament
Myosin arm

Figure 6.27 A

(a) Myosin motors in muscle cell contraction.

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Amoeboid movement
Involves the contraction of actin and myosin
filaments
Cortex (outer cytoplasm):
gel with actin network
Inner cytoplasm: sol
with actin subunits
Extending
pseudopodium

Figure 6.27 B

(b) Amoeboid movement

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Cytoplasmic streaming
Is another form of locomotion created by
microfilaments
Nonmoving
cytoplasm (gel)
Chloroplast
Streaming
cytoplasm
(sol)

Parallel actin
filaments

Figure 6.27 C

(b) Cytoplasmic streaming in plant cells

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Cell wall

Intermediate Filaments
Intermediate filaments
Support cell shape
Fix organelles in place

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Concept 6.7: Extracellular components and


connections between cells help coordinate
cellular activities

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Cell Walls of Plants


The cell wall
Is an extracellular structure of plant cells that
distinguishes them from animal cells

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Plant cell walls


Are made of cellulose fibers embedded in
other polysaccharides and protein
May have multiple layers
Central
vacuole
of cell

Plasma
membrane
Secondary
cell wall
Primary
cell wall

Central
vacuole
of cell

Middle
lamella
1 m
Central vacuole

Cytosol
Plasma membrane

Plant cell walls

Figure 6.28
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Plasmodesmata

The Extracellular Matrix (ECM) of Animal Cells


Animal cells
Lack cell walls
Are covered by an elaborate matrix, the ECM

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The ECM
Is made up of glycoproteins and other
macromolecules
EXTRACELLULAR FLUID
Collagen

A proteoglycan
complex

Polysaccharide
molecule
Carbohydrates
Core
protein

Fibronectin

Plasma
membrane

Integrin

Figure 6.29

Integrins

Microfilaments

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CYTOPLASM

Proteoglycan
molecule

Functions of the ECM include


Support
Adhesion
Movement
Regulation

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Intercellular Junctions

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Plants: Plasmodesmata
Plasmodesmata
Are channels that perforate plant cell walls
Cell walls

Interior
of cell

Interior
of cell
Figure 6.30

0.5 m

Plasmodesmata

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Plasma membranes

Animals: Tight Junctions, Desmosomes, and Gap Junctions

In animals, there are three types of intercellular


junctions
Tight junctions
Desmosomes
Gap junctions

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Types of intercellular junctions in animals


TIGHT JUNCTIONS
Tight junction

Tight junctions prevent


fluid from moving
across a layer of cells

0.5 m

At tight junctions, the membranes of


neighboring cells are very tightly pressed
against each other, bound together by
specific proteins (purple). Forming continuous seals around the cells, tight junctions
prevent leakage of extracellular fluid across
A layer of epithelial cells.

DESMOSOMES
Desmosomes (also called anchoring
junctions) function like rivets, fastening cells
Together into strong sheets. Intermediate
Filaments made of sturdy keratin proteins
Anchor desmosomes in the cytoplasm.

Tight junctions
Intermediate
filaments
Desmosome
Gap
junctions

Space
between Plasma membranes
cells
of adjacent cells

1 m

Extracellular
matrix
Gap junction

Figure 6.31
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0.1 m

GAP JUNCTIONS
Gap junctions (also called communicating
junctions) provide cytoplasmic channels from
one cell to an adjacent cell. Gap junctions
consist of special membrane proteins that
surround a pore through which ions, sugars,
amino acids, and other small molecules may
pass. Gap junctions are necessary for communication between cells in many types of tissues,
including heart muscle and animal embryos.

The Cell: A Living Unit Greater Than the Sum of Its Parts

5 m

Cells rely on the integration of structures and


organelles in order to function

Figure 6.32
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