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Figure 6.1
Copyright 2005 Pearson Education, Inc. publishing as Benjamin Cummings
10 m
Microscopy
Scientists use microscopes to visualize cells
too small to see with the naked eye
Unaided eye
Length of some
nerve and
muscle cells
Chicken egg
1 cm
100 m
10 m
1m
100 nm
Frog egg
Most plant
and Animal
cells
Nucleus
Most bacteria
Mitochondrion
Smallest bacteria
Viruses
10 nm
Ribosomes
Proteins
1 nm
Lipids
Small molecules
Figure 6.2
0.1 nm
Atoms
Electron microscope
1 mm
Electron microscope
0.1 m
Human height
Light microscope
1m
Measurements
1 centimeter (cm) = 102 meter (m) = 0.4 inch
1 millimeter (mm) = 103 m
1 micrometer (m) = 103 mm = 106 m
1 nanometer (nm) = 103 mm = 109 m
RESULT
Figure 6.3
Copyright 2005 Pearson Education, Inc. publishing as Benjamin Cummings
50 m
50 m
Copyright 2005 Pearson Education, Inc. publishing as Benjamin Cummings
RESULTS
1 m
Cilia
(a) Scanning electron microscopy (SEM). Micrographs taken
with a scanning electron microscope show a 3D image of the
surface of a specimen. This SEM
shows the surface of a cell from a
rabbit trachea (windpipe) covered
with motile organelles called cilia.
Beating of the cilia helps move
inhaled debris upward toward
the throat.
Cross section
of cilium
1 m
The centrifuge
Is used to fractionate cells into their
component parts
Figure 6.5
Copyright 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Homogenization
Tissue
cells
1000 g
Homogenate
(1000 times the
force of gravity)
Differential centrifugation
10 min
Supernatant poured
into next tube
20,000 g
20 min
Pellet rich in
nuclei and
cellular debris
Figure 6.5
80,000 g
60 min
150,000 g
3 hr
Pellet rich in
mitochondria
(and chloroplasts if cells
are from a Pellet rich in
plant)
microsomes
(pieces of
plasma membranes and
Pellet rich in
cells internal ribosomes
membranes)
Figure 6.5
Copyright 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Prokaryotic cells
Do not contain a nucleus
Have their DNA located in a region called
the nucleoid
Bacterial
chromosome
(a) A typical
rod-shaped bacterium
Figure 6.6 A, B
Copyright 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Eukaryotic cells
Contain a true nucleus, bounded by a
membranous nuclear envelope
Are generally quite a bit bigger than
prokaryotic cells
A smaller cell
Has a higher surface to volume ratio, which
facilitates the exchange of materials into and
out of the cell
Surface area increases while
total volume remains constant
5
1
1
Total surface area
(height width
number of sides
number of boxes)
150
750
Total volume
(height width length
number of boxes)
125
125
Surface-to-volume
ratio
(surface area volume)
12
Figure 6.7
Copyright 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Hydrophilic
region
Inside of cell
Figure 6.8 A, B
0.1 m
Hydrophobic
region
Hydrophilic
region
Phospholipid
Proteins
A animal cell
ENDOPLASMIC RETICULUM (ER)
Rough ER
Smooth ER
Nuclear envelope
Nucleolus
NUCLEUS
Chromatin
Flagelium
Plasma membrane
Centrosome
CYTOSKELETON
Microfilaments
Intermediate filaments
Ribosomes
Microtubules
Microvilli
Golgi apparatus
Peroxisome
Figure 6.9
Mitochondrion
Lysosome
A plant cell
Nuclear envelope
Nucleolus
Chromatin
NUCLEUS
Centrosome
Rough
endoplasmic
reticulum Smooth
endoplasmic
reticulum
Central vacuole
Tonoplast
Golgi apparatus
Microfilaments
Intermediate
filaments
CYTOSKELETON
Microtubules
Mitochondrion
Peroxisome
Plasma membrane
Chloroplast
Cell wall
Plasmodesmata
Wall of adjacent cell
Figure 6.9
Copyright 2005 Pearson Education, Inc. publishing as Benjamin Cummings
1 m
Nucleolus
Chromatin
Nucleus
Nuclear envelope:
Inner membrane
Outer membrane
Nuclear pore
Pore
complex
Rough ER
Surface of nuclear
envelope.
1 m
Ribosome
0.25 m
Close-up of
nuclear
envelope
Figure 6.10
ER
Cytosol
Endoplasmic reticulum (ER)
Free ribosomes
Bound ribosomes
Large
subunit
0.5 m
TEM showing ER and ribosomes
Figure 6.11
Copyright 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Small
subunit
Diagram of a ribosome
The ER membrane
Is continuous with the nuclear envelope
Smooth ER
Rough ER
Nuclear
envelope
ER lumen
Cisternae
Ribosomes
Transitional ER
Transport vesicle
Smooth ER
Rough ER 200 m
Figure 6.12
Copyright 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Functions of Smooth ER
The smooth ER
Synthesizes lipids
Metabolizes carbohydrates
Stores calcium
Detoxifies poison
Functions of Rough ER
The rough ER
Has bound ribosomes
Produces proteins and membranes, which are
distributed by transport vesicles
1 Vesicles move
2 Vesicles coalesce to
6 Vesicles also
form new cis Golgi cisternae
from ER to Golgi
transport certain
Cisternae
proteins back to ER
3 Cisternal
maturation:
Golgi cisternae
move in a cisto-trans
direction
Figure 6.13
5 Vesicles transport specific
proteins backward to newer
Golgi cisternae
trans face
(shipping side of
Golgi apparatus)
0.1 0 m
1 m
Lysosome
Lysosome contains
active hydrolytic
enzymes
Food vacuole
fuses with
lysosome
Hydrolytic
enzymes digest
food particles
Digestive
enzymes
Lysosome
Plasma membrane
Digestion
Food vacuole
Figure 6.14 A
Copyright 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Autophagy
Lysosome containing
two damaged organelles
1m
Mitochondrion
fragment
Peroxisome
fragment
Hydrolytic enzymes
digest organelle
components
Lysosome
Figure 6.14 B
Vesicle containing
damaged mitochondrion
Digestion
Food vacuoles
Are formed by phagocytosis
Contractile vacuoles
Pump excess water out of protist cells
Central vacuoles
Are found in plant cells
Hold reserves of important organic
compounds and water
Central vacuole
Cytosol
Tonoplast
Nucleus
Central
vacuole
Cell wall
Chloroplast
Figure 6.15
Copyright 2005 Pearson Education, Inc. publishing as Benjamin Cummings
5 m
Nucleus
Rough ER
Smooth ER
cis Golgi
Nuclear envelop
Plasma
membrane
trans Golgi
Lysosome available
for fusion with another
vesicle for digestion
Figure 6.16
Copyright 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Chloroplasts
Found only in plants, are the sites of
photosynthesis
Free
ribosomes
in the
mitochondrial
matrix
Inner
membrane
Cristae
Matrix
Figure 6.17
Mitochondrial
DNA
100 m
Chloroplasts
Are found in leaves and other green organs of
plants and in algae
Chloroplast
Ribosomes
Stroma
Chloroplast
DNA
Figure 6.18
Thylakoid
Peroxisomes: Oxidation
Peroxisomes
Produce hydrogen peroxide and convert it to
water
Chloroplast
Peroxisome
Mitochondrion
Figure 6.19
1 m
Copyright 2005 Pearson Education, Inc. publishing as Benjamin Cummings
The cytoskeleton
Is a network of fibers extending throughout the
cytoplasm
Microtubule
Figure 6.20
Copyright 2005 Pearson Education, Inc. publishing as Benjamin Cummings
0.25 m
Microfilaments
The cytoskeleton
Gives mechanical support to the cell
Vesicle
Receptor for
motor protein
Motor protein
Microtubule
(ATP powered)
of cytoskeleton
(a) Motor proteins that attach to receptors on organelles can walk
the organelles along microtubules or, in some cases, microfilaments.
Vesicles
Microtubule
0.25 m
Figure 6.21 A, B
Table 6.1
Copyright 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Microtubules
Microtubules
Shape the cell
Guide movement of organelles
Help separate the chromosome copies in
dividing cells
Microtubule
Centrioles
0.25 m
Figure 6.22
Longitudinal section
of one centriole
Microtubules
Cross section
of the other centriole
Direction of swimming
Figure 6.23 A
1 m
Ciliary motion
(b) Motion of cilia. Cilia have a backand-forth motion that moves the
cell in a direction perpendicular
to the axis of the cilium. A dense
nap of cilia, beating at a rate of
about 40 to 60 strokes a second,
covers this Colpidium, a
freshwater protozoan (SEM).
Figure 6.23 B
15 m
0.1 m
Central
microtubule
Outer doublets
cross-linking
proteins inside
Microtubules
Radial
spoke
Plasma
membrane
Basal body
0.5 m
(a)
(b)
0.1 m
Triplet
(c)
Plasma
membrane
ATP
Dynein arm
(a) Powered by ATP, the dynein arms of one microtubule doublet
grip the adjacent doublet, push it up, release, and then grip again.
If the two microtubule doublets were not attached, they would slide
relative to each other.
Figure 6.25 A
Copyright 2005 Pearson Education, Inc. publishing as Benjamin Cummings
ATP
Outer doublets
cross-linking
proteins
Anchorage
in cell
(b) In a cilium or flagellum, two adjacent doublets cannot slide far because
they are physically restrained by proteins, so they bend. (Only two of
the nine outer doublets in Figure 6.24b are shown here.)
Figure 6.25 B
Copyright 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Plasma membrane
Microfilaments (actin
filaments)
Intermediate filaments
Figure 6.26
Copyright 2005 Pearson Education, Inc. publishing as Benjamin Cummings
0.25 m
Muscle cell
Actin filament
Myosin filament
Myosin arm
Figure 6.27 A
Amoeboid movement
Involves the contraction of actin and myosin
filaments
Cortex (outer cytoplasm):
gel with actin network
Inner cytoplasm: sol
with actin subunits
Extending
pseudopodium
Figure 6.27 B
Cytoplasmic streaming
Is another form of locomotion created by
microfilaments
Nonmoving
cytoplasm (gel)
Chloroplast
Streaming
cytoplasm
(sol)
Parallel actin
filaments
Figure 6.27 C
Cell wall
Intermediate Filaments
Intermediate filaments
Support cell shape
Fix organelles in place
Plasma
membrane
Secondary
cell wall
Primary
cell wall
Central
vacuole
of cell
Middle
lamella
1 m
Central vacuole
Cytosol
Plasma membrane
Figure 6.28
Copyright 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Plasmodesmata
The ECM
Is made up of glycoproteins and other
macromolecules
EXTRACELLULAR FLUID
Collagen
A proteoglycan
complex
Polysaccharide
molecule
Carbohydrates
Core
protein
Fibronectin
Plasma
membrane
Integrin
Figure 6.29
Integrins
Microfilaments
CYTOPLASM
Proteoglycan
molecule
Intercellular Junctions
Plants: Plasmodesmata
Plasmodesmata
Are channels that perforate plant cell walls
Cell walls
Interior
of cell
Interior
of cell
Figure 6.30
0.5 m
Plasmodesmata
Plasma membranes
0.5 m
DESMOSOMES
Desmosomes (also called anchoring
junctions) function like rivets, fastening cells
Together into strong sheets. Intermediate
Filaments made of sturdy keratin proteins
Anchor desmosomes in the cytoplasm.
Tight junctions
Intermediate
filaments
Desmosome
Gap
junctions
Space
between Plasma membranes
cells
of adjacent cells
1 m
Extracellular
matrix
Gap junction
Figure 6.31
Copyright 2005 Pearson Education, Inc. publishing as Benjamin Cummings
0.1 m
GAP JUNCTIONS
Gap junctions (also called communicating
junctions) provide cytoplasmic channels from
one cell to an adjacent cell. Gap junctions
consist of special membrane proteins that
surround a pore through which ions, sugars,
amino acids, and other small molecules may
pass. Gap junctions are necessary for communication between cells in many types of tissues,
including heart muscle and animal embryos.
The Cell: A Living Unit Greater Than the Sum of Its Parts
5 m
Figure 6.32
Copyright 2005 Pearson Education, Inc. publishing as Benjamin Cummings