Progress toward developing nature-inspired and low-cost lignocelluloses

processing technologies
Shulin Chen, Innu Chaudhary, Shuai Zhang, Sujala Bhattarai, Mohammadali Azadfar, Xiao Fu, Allan Gao
Biomass Process and Bioproduct Engineering Laboratory (BBEL), Washington State University, Pullman, WA 99164, USA

Program Goal and Specific Aims

Goal
Develop more environmentally benign,
energy efficient, and downstream conversion
friendly processes for liberating sugars from
lignocellulosic biomass
Specific Aims
Investigating
plant
cell
wall
deconstruction process in termite, with
focus on the foregut and midgut;
Studying enzyme systems and processes
of novel white rot fungi for lignin
deconstruction;
Characterizing
lignin
modification,
degradation and derivatives in the above
processes;
Engineering nature-inspired pretreatment
processes for industrial applications.

Current Research Efforts

Investigating
radical
reaction
mechanisms in Termite
Proteomics studies of key enzyme
systems in termite for lignin
modification
Proteomics studies of key enzyme
systems in white rote fungi for lignin
modification
Modeling and simulation of chemical
bond disassociation of lignin by
radicals
High value utilization of lignin
derivatives (Session ???)
OASS process development and
optimization (Session ???)
OASS process development and
modeling (Session ???)

Results
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Conclusions
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Objectives
the natural biological systems have developed
various special systems for breaking down
plant cell walls. The systems include three
components: (1) the chemical environments
that maintain the conditions under which the
reaction occurs; (2) the enzyme systems that
create radicals to reaction with lignin and
modify the hydrogen bond system to allow
cellulytic enzyme to access the hemicellulos
and cellulose; (3) the enzyme systems that
process the cellulose and hemicellulose and
other components. The radical generation
system is a type of Fenton reaction system.
For different structures of substrate or
resulted structure after pretreatment, there is
an optimal enzyme system for the
deconstruction of the cell wall. The radical
process and the enzymatic process can be
mimicked with chemical reaction systems.

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Anxiety

Depression

-Anxiety is so physical
-Thought I was having a heart
attack
-Rapid breathing
-Sweating palms
-Cant sleep
-Nausea

-Tired
-Exhaustion
-Loss of appetite

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Cognitive

-Racing thoughts

-Downward spiral
-Lack of motivation
-Pessimism
-Worthlessness
-Suicidal thoughts

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Behavioral

-Restlessness
- Cant sit still
-Nail biting
-Hair pulling
-You do anything to get rid of it

-Tearfulness
-Isolation
-Poor personal hygiene

Affective

-Desperation
-Fear
-No peace
-Urgency

-Sadness
-Darkness
-Hopelessness

Physiological

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Acknowledgments
NSF
Washington State University
Jill McCluskey-School of Economic
Sciences, Washington State University
Cornelius Ivory- Voiland School of
Chemical Engineering and Bioengineering,
Washington State University
Lawrence Que, Jr.- Department of
Chemistry, University of Minnesota;
Joy Doran-Peterson, Department of
Microbiology, University of Georgia