Beruflich Dokumente
Kultur Dokumente
Gas Chromatography
Filters/Traps
Data system
H
RESET
Regulators
Syringe/Sampler
Inlets
Detectors
Gas Carrier
Hydrogen
Air
Column
gas
system
inlet
column
detector
data
system
DETECTORS
Exhaust
Chimney
Igniter
Hydrogen
Inlet
Collector Electrode
Polarizing Electrode
Column
Effluent
Detector electronics
- 220 volts
Flame
Chassis ground
Jet
Column
Signal output
Thermal Conductivity
Detector
Principal: The thermal balance of a heated
filament
Electrical power is converted to heat in a
resistant filament and the temperature will
climb until heat power loss form the
filament equals the electrical power input.
The filament may loose heat by radiation to
a cooler surface and by conduction to the
molecules coming into contact with it.
Flow
Flow
Carbon Tetrachloride
Benzene
Hexane
Argon
Methanol
Nitrogen
Helium
Hydrogen
Relative Thermal
Conductivity
0.05
0.11
0.12
0.12
0.13
0.17
1.00
1.28
H or
63
Gas Chromatography
Application
C18
C 16
8
6
C14
4
2
0.5
1.0
1.5
2.0
2.5
Retention Time
The content % of C14 fatty acids =
C
C + C + C
3.0
Response
Mixture of known compounds
Octane
1.6 min = RT
Hexane
Response
1.6 min = RT
Decane
Retention Times
Response
Response
GLC ADVANTAGES
1. Very good separation
2. Time (analysis is short)
3. Small sample is needed - l
4. Good detection system
5. Quantitatively analyzed
Methylester
Fatty Acids
R C OH + CH3 OH + H2 SO4
Reflux
R C O CH3
Volatile in Gas
Chromatography
O
CH2 O C R
O
CH
O C R
O
CH2
O C R
CH3 ONa
+
CH3 OH
O
3 R C O CH3
Volatile in Gas
Chromatography
4
OH
HO
3
6
CH2OH
O
5
2 OH 4
OH
OH
HO
3
1
2 OH
OH
4
OH
HO
3
CH
1 +
Si
5Cl
2 OH
OH
CH
Glucose
4
(CH3)3-Si-O
3
CH
Trimethylchlorosilane
6
CH2O-Si(CH3)3
O
5
O-Si(CH ) 1
3 3
2 O-Si(CH3)3
O-Si(CH3)3
5HCl
Effects of Derivation
1.Time
consumption
2.Side reaction
3.Loss of sample
SOLVENT
Solvent
Front
1.1 cm
5.5 cm
Origin
Rf =
= 0.5